Persimmon cryptic virus
Average proteome isoelectric point is 5.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I4EPL9|I4EPL9_9VIRU Coat protein OS=Persimmon cryptic virus OX=1183241 GN=CP PE=4 SV=1
MM1 pKa = 7.9 ALRR4 pKa = 11.84 SITGYY9 pKa = 10.15 EE10 pKa = 3.73 FHH12 pKa = 7.74 DD13 pKa = 4.06 FQSSLEE19 pKa = 4.3 LLNQTHH25 pKa = 5.14 IHH27 pKa = 5.36 IVRR30 pKa = 11.84 RR31 pKa = 11.84 EE32 pKa = 4.06 SGVTYY37 pKa = 10.15 HH38 pKa = 7.71 DD39 pKa = 3.92 EE40 pKa = 4.1 FALCEE45 pKa = 4.03 LLVDD49 pKa = 3.6 NTRR52 pKa = 11.84 LYY54 pKa = 10.15 EE55 pKa = 3.97 QEE57 pKa = 3.94 LEE59 pKa = 4.18 GWSRR63 pKa = 11.84 SYY65 pKa = 10.01 YY66 pKa = 10.1 TGEE69 pKa = 3.75 QHH71 pKa = 6.39 MKK73 pKa = 10.8 AILQYY78 pKa = 10.62 SLPNTPIQDD87 pKa = 3.17 IDD89 pKa = 3.78 VGCYY93 pKa = 6.98 QQAMTNVQEE102 pKa = 4.56 RR103 pKa = 11.84 LSSLPIVRR111 pKa = 11.84 AFDD114 pKa = 3.43 VLTQLDD120 pKa = 3.83 QVSFEE125 pKa = 4.25 SSSAAGYY132 pKa = 10.44 DD133 pKa = 3.42 YY134 pKa = 11.25 TGAKK138 pKa = 9.43 GPKK141 pKa = 9.39 NEE143 pKa = 4.37 GNHH146 pKa = 4.54 EE147 pKa = 3.95 RR148 pKa = 11.84 AIRR151 pKa = 11.84 RR152 pKa = 11.84 AKK154 pKa = 9.99 AVLWSAIAQDD164 pKa = 4.06 GEE166 pKa = 4.64 GIEE169 pKa = 4.26 HH170 pKa = 6.53 VLRR173 pKa = 11.84 SSVPDD178 pKa = 3.17 VGYY181 pKa = 8.77 TRR183 pKa = 11.84 TQLTDD188 pKa = 3.48 LSEE191 pKa = 4.12 KK192 pKa = 9.74 TKK194 pKa = 10.6 VRR196 pKa = 11.84 GVWGRR201 pKa = 11.84 AFHH204 pKa = 6.63 YY205 pKa = 9.76 ILPEE209 pKa = 4.15 GTSADD214 pKa = 3.84 PLLQAFKK221 pKa = 10.87 EE222 pKa = 4.45 GGTFYY227 pKa = 10.77 HH228 pKa = 7.2 IGQDD232 pKa = 3.49 PTVSVPYY239 pKa = 10.01 ILSDD243 pKa = 3.6 TAGKK247 pKa = 9.67 CAWLYY252 pKa = 11.14 ALDD255 pKa = 3.85 WSSFDD260 pKa = 3.26 ATVSRR265 pKa = 11.84 FEE267 pKa = 3.48 IHH269 pKa = 7.07 AAFDD273 pKa = 3.93 LLKK276 pKa = 10.62 QRR278 pKa = 11.84 IEE280 pKa = 4.08 FPNFEE285 pKa = 4.2 TEE287 pKa = 3.56 QCYY290 pKa = 9.72 EE291 pKa = 3.47 ICRR294 pKa = 11.84 QLFIHH299 pKa = 6.58 KK300 pKa = 9.99 KK301 pKa = 8.91 IAAPDD306 pKa = 3.68 GKK308 pKa = 10.84 VYY310 pKa = 9.56 WAHH313 pKa = 6.77 KK314 pKa = 8.99 GIPSGSYY321 pKa = 6.92 YY322 pKa = 10.46 TSIIGSIINRR332 pKa = 11.84 LRR334 pKa = 11.84 IEE336 pKa = 4.37 YY337 pKa = 9.63 IWIKK341 pKa = 10.68 LRR343 pKa = 11.84 GHH345 pKa = 6.83 GPTICYY351 pKa = 7.61 TQGDD355 pKa = 4.29 DD356 pKa = 4.56 SLCGDD361 pKa = 4.47 DD362 pKa = 5.6 EE363 pKa = 5.59 RR364 pKa = 11.84 IEE366 pKa = 4.09 PEE368 pKa = 4.58 RR369 pKa = 11.84 IADD372 pKa = 3.46 IANPIGWLINPAKK385 pKa = 9.63 TATTRR390 pKa = 11.84 YY391 pKa = 9.02 PEE393 pKa = 4.52 YY394 pKa = 9.68 ITFLGRR400 pKa = 11.84 TCYY403 pKa = 10.48 GGLNHH408 pKa = 7.54 RR409 pKa = 11.84 DD410 pKa = 3.64 LIRR413 pKa = 11.84 CLRR416 pKa = 11.84 LLIYY420 pKa = 10.05 PEE422 pKa = 4.19 YY423 pKa = 9.12 PVPSGAISAYY433 pKa = 8.96 RR434 pKa = 11.84 ANSIAEE440 pKa = 4.24 DD441 pKa = 3.75 CGGTSSILNDD451 pKa = 2.94 IARR454 pKa = 11.84 RR455 pKa = 11.84 LTRR458 pKa = 11.84 KK459 pKa = 9.33 YY460 pKa = 10.34 GRR462 pKa = 11.84 VSHH465 pKa = 6.5 EE466 pKa = 4.1 EE467 pKa = 3.85 VPKK470 pKa = 9.45 EE471 pKa = 3.62 LRR473 pKa = 11.84 VYY475 pKa = 10.48 RR476 pKa = 11.84 HH477 pKa = 5.74
Molecular weight: 54.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.685
IPC2_protein 5.741
IPC_protein 5.792
Toseland 6.186
ProMoST 6.084
Dawson 6.071
Bjellqvist 6.071
Wikipedia 6.084
Rodwell 6.059
Grimsley 6.338
Solomon 6.071
Lehninger 6.071
Nozaki 6.338
DTASelect 6.532
Thurlkill 6.547
EMBOSS 6.504
Sillero 6.44
Patrickios 3.681
IPC_peptide 6.084
IPC2_peptide 6.44
IPC2.peptide.svr19 6.379
Protein with the highest isoelectric point:
>tr|I4EPL9|I4EPL9_9VIRU Coat protein OS=Persimmon cryptic virus OX=1183241 GN=CP PE=4 SV=1
MM1 pKa = 7.94 ADD3 pKa = 3.05 QGNNEE8 pKa = 4.22 TPLPVQATQPAANSDD23 pKa = 4.43 RR24 pKa = 11.84 IPITTLPAPRR34 pKa = 11.84 SQAPNKK40 pKa = 9.78 PEE42 pKa = 3.77 YY43 pKa = 10.89 ARR45 pKa = 11.84 IADD48 pKa = 4.0 SLEE51 pKa = 4.12 DD52 pKa = 3.71 EE53 pKa = 4.49 YY54 pKa = 11.5 QIGFQRR60 pKa = 11.84 INPRR64 pKa = 11.84 LHH66 pKa = 5.76 RR67 pKa = 11.84 RR68 pKa = 11.84 YY69 pKa = 10.4 VILRR73 pKa = 11.84 SQEE76 pKa = 3.73 LHH78 pKa = 7.43 DD79 pKa = 4.51 RR80 pKa = 11.84 LCNLYY85 pKa = 10.58 APYY88 pKa = 9.79 FQAGYY93 pKa = 10.23 RR94 pKa = 11.84 VFKK97 pKa = 10.53 HH98 pKa = 6.09 VIEE101 pKa = 5.3 AYY103 pKa = 9.25 PLPAGYY109 pKa = 10.54 ASQIAYY115 pKa = 7.69 ATACYY120 pKa = 9.86 VSSWCWDD127 pKa = 3.51 LYY129 pKa = 10.04 VSIRR133 pKa = 11.84 EE134 pKa = 4.27 SVKK137 pKa = 10.69 KK138 pKa = 10.84 LSQTAFLQQYY148 pKa = 6.6 QTEE151 pKa = 4.4 QFHH154 pKa = 6.07 SQDD157 pKa = 3.19 RR158 pKa = 11.84 YY159 pKa = 11.57 DD160 pKa = 4.73 PFLQHH165 pKa = 6.96 LNTVIRR171 pKa = 11.84 PTHH174 pKa = 5.84 IPGGLEE180 pKa = 3.76 DD181 pKa = 4.13 SLYY184 pKa = 10.52 IPLIANHH191 pKa = 5.71 VQWGNQSPFGINHH204 pKa = 5.36 YY205 pKa = 10.26 AINEE209 pKa = 4.24 SLVQGLLDD217 pKa = 5.32 VMDD220 pKa = 5.37 LKK222 pKa = 11.49 NNTWRR227 pKa = 11.84 TVPLSHH233 pKa = 7.27 DD234 pKa = 3.54 TLGRR238 pKa = 11.84 PMWLLDD244 pKa = 3.03 WRR246 pKa = 11.84 QNQAYY251 pKa = 9.98 AWFPQEE257 pKa = 3.7 NHH259 pKa = 6.09 FSKK262 pKa = 10.93 EE263 pKa = 3.84 DD264 pKa = 3.83 LIAAHH269 pKa = 7.09 ILGIPCSPRR278 pKa = 11.84 LGPRR282 pKa = 11.84 DD283 pKa = 3.32 VDD285 pKa = 3.13 DD286 pKa = 3.75 WQFFPGNVMPQHH298 pKa = 6.32 INIAAYY304 pKa = 9.43 EE305 pKa = 4.02 RR306 pKa = 11.84 AIPRR310 pKa = 11.84 RR311 pKa = 11.84 FFGAAEE317 pKa = 3.92 YY318 pKa = 8.56 RR319 pKa = 11.84 TISTRR324 pKa = 11.84 MWEE327 pKa = 4.44 LPFLSAVAGQMSGAKK342 pKa = 9.71 RR343 pKa = 11.84 PAPEE347 pKa = 4.11 SSATGSGYY355 pKa = 9.26 MEE357 pKa = 4.74 SPPAVEE363 pKa = 4.62 GQQTAAIMDD372 pKa = 4.47 PGIMQTRR379 pKa = 11.84 YY380 pKa = 9.48 CQYY383 pKa = 10.96 QIIDD387 pKa = 3.29 WCYY390 pKa = 8.43 HH391 pKa = 4.39 GRR393 pKa = 11.84 VILNLNAQKK402 pKa = 10.58 QNQALRR408 pKa = 11.84 SILFSPNN415 pKa = 2.57
Molecular weight: 47.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.209
IPC2_protein 6.224
IPC_protein 6.351
Toseland 6.649
ProMoST 6.751
Dawson 6.722
Bjellqvist 6.664
Wikipedia 6.737
Rodwell 6.707
Grimsley 6.795
Solomon 6.722
Lehninger 6.722
Nozaki 6.985
DTASelect 7.161
Thurlkill 7.19
EMBOSS 7.161
Sillero 7.102
Patrickios 3.63
IPC_peptide 6.737
IPC2_peptide 7.015
IPC2.peptide.svr19 6.902
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
892
415
477
446.0
50.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.072 ± 0.697
1.794 ± 0.224
5.045 ± 0.3
5.717 ± 0.887
3.363 ± 0.161
6.278 ± 0.783
3.251 ± 0.076
7.511 ± 0.491
2.803 ± 0.562
8.408 ± 0.138
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.345 ± 0.529
3.812 ± 0.802
6.054 ± 1.22
5.83 ± 1.364
6.839 ± 0.214
6.726 ± 0.297
5.157 ± 0.682
4.372 ± 0.177
2.018 ± 0.252
5.605 ± 0.196
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here