Zoogloea oleivorans
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4972 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6C2D181|A0A6C2D181_9RHOO 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase OS=Zoogloea oleivorans OX=1552750 GN=uraD PE=4 SV=1
MM1 pKa = 7.58 SGGGDD6 pKa = 3.3 VTRR9 pKa = 11.84 FTIDD13 pKa = 3.39 PDD15 pKa = 3.49 TGALSFLTAPDD26 pKa = 3.86 FEE28 pKa = 4.7 VPQDD32 pKa = 3.27 EE33 pKa = 4.83 GANNVYY39 pKa = 10.6 DD40 pKa = 4.36 VIVQVMDD47 pKa = 4.18 GANSTAIQAITVTVTAVNDD66 pKa = 3.6 NAPVLTSNGGGVMAAVTIAEE86 pKa = 4.41 STTLVTTLTATDD98 pKa = 3.7 SDD100 pKa = 4.53 LPGDD104 pKa = 3.66 TLYY107 pKa = 11.42 YY108 pKa = 10.34 SIVGGADD115 pKa = 2.98 AAKK118 pKa = 10.0 FAIDD122 pKa = 3.79 TFSGALNFALAPDD135 pKa = 3.96 YY136 pKa = 10.86 DD137 pKa = 3.97 LPGDD141 pKa = 3.65 VGGDD145 pKa = 3.46 NIYY148 pKa = 11.15 DD149 pKa = 3.69 VIVRR153 pKa = 11.84 VDD155 pKa = 3.45 DD156 pKa = 3.35 GTYY159 pKa = 10.97 AVNQSLAITVSNTNEE174 pKa = 3.89 APAVTITSTSYY185 pKa = 9.72 TATEE189 pKa = 3.81 QSALVLGGTGLSISDD204 pKa = 4.08 ADD206 pKa = 3.86 AGSHH210 pKa = 5.08 VVSARR215 pKa = 11.84 LNVVSGTLSATAGSSGVTITGSGTASLVFDD245 pKa = 3.97 GTLAQINDD253 pKa = 3.78 FLAGKK258 pKa = 9.94 LGASLTYY265 pKa = 10.55 LIDD268 pKa = 4.02 IDD270 pKa = 4.49 TPPASDD276 pKa = 3.86 TLTLAADD283 pKa = 4.26 DD284 pKa = 4.9 LGHH287 pKa = 6.6 TGSGTAASGSKK298 pKa = 10.23 SVLINITAVNDD309 pKa = 4.13 GPANTTPGTQVTLVNTPLVFGVSHH333 pKa = 6.63 GNALSVTDD341 pKa = 4.51 SDD343 pKa = 4.42 AAANPLQISLVATHH357 pKa = 6.43 GTLTLGNTTGLTFVSGDD374 pKa = 3.37 GAADD378 pKa = 3.25 AGMTFTGTQASINAALEE395 pKa = 4.28 GLSYY399 pKa = 10.79 TPTAAYY405 pKa = 8.09 TGTATISLGSADD417 pKa = 3.71 QGNTGGGVLSDD428 pKa = 3.82 SDD430 pKa = 4.38 TINIQIGASRR440 pKa = 11.84 YY441 pKa = 6.8 QQGLDD446 pKa = 3.62 GYY448 pKa = 9.17 TGMEE452 pKa = 4.13 DD453 pKa = 3.55 TYY455 pKa = 10.96 LDD457 pKa = 3.79 SVTPDD462 pKa = 2.9 SSYY465 pKa = 11.97 GNASTVLVDD474 pKa = 4.39 DD475 pKa = 5.53 GSPVSQGLLRR485 pKa = 11.84 FDD487 pKa = 5.25 GIFGDD492 pKa = 4.65 GVGQIPFGSTINSASLSFYY511 pKa = 10.96 VINQDD516 pKa = 3.35 AADD519 pKa = 3.99 SVSLHH524 pKa = 5.8 TMLTNWTEE532 pKa = 3.71 NATWNTLGAGIQTNGVEE549 pKa = 4.35 AVGTPVATFSAGASGWNTIGGLDD572 pKa = 3.69 ASVQIWANGGANYY585 pKa = 9.89 GWSLLTANPGADD597 pKa = 2.6 SWTFASSEE605 pKa = 4.09 YY606 pKa = 9.78 STLNLRR612 pKa = 11.84 PYY614 pKa = 10.85 LVITYY619 pKa = 7.4 TPPEE623 pKa = 4.19 APVITSNGGGASGVVSVPEE642 pKa = 3.99 NTTSVTTVTATDD654 pKa = 3.57 ADD656 pKa = 3.93 SAQTVLRR663 pKa = 11.84 YY664 pKa = 10.2 SIVGGADD671 pKa = 3.05 STRR674 pKa = 11.84 FSINEE679 pKa = 3.97 TTGVLSFLTAPDD691 pKa = 4.02 FEE693 pKa = 5.25 NPLDD697 pKa = 3.56 IGGNNIYY704 pKa = 10.43 DD705 pKa = 3.43 ITVQVSDD712 pKa = 4.52 GLLSSTQVLAVSVGAVNDD730 pKa = 3.72 NAPLITIPAAVWITEE745 pKa = 3.72 NSTAVATITVSDD757 pKa = 4.05 SDD759 pKa = 4.12 RR760 pKa = 11.84 PAQSFSYY767 pKa = 10.42 SLAGGVDD774 pKa = 3.16 AALFTINTSTGDD786 pKa = 3.76 LRR788 pKa = 11.84 FLSAPDD794 pKa = 3.76 FEE796 pKa = 5.3 SPLDD800 pKa = 3.84 AGGDD804 pKa = 3.45 NVYY807 pKa = 10.93 DD808 pKa = 3.47 VTVQVSDD815 pKa = 3.77 SDD817 pKa = 4.28 GSTSTQAIAVTVTSADD833 pKa = 3.33 DD834 pKa = 3.58 SAPVITSPSVVFVAEE849 pKa = 3.94 NTTEE853 pKa = 3.89 VMTITASDD861 pKa = 3.84 ADD863 pKa = 3.89 QPAQALSYY871 pKa = 10.47 SLAGGADD878 pKa = 3.44 VARR881 pKa = 11.84 FTIDD885 pKa = 3.25 PVSGILRR892 pKa = 11.84 FVRR895 pKa = 11.84 APGFGVPADD904 pKa = 3.71 ANRR907 pKa = 11.84 DD908 pKa = 3.36 NVYY911 pKa = 10.96 DD912 pKa = 3.96 VIVRR916 pKa = 11.84 VSDD919 pKa = 3.83 GVLTGTQALSVSVEE933 pKa = 4.0 NRR935 pKa = 11.84 NEE937 pKa = 3.89 MPQGRR942 pKa = 11.84 DD943 pKa = 2.86 GRR945 pKa = 11.84 VQMLEE950 pKa = 4.38 DD951 pKa = 3.7 GTHH954 pKa = 6.06 TFTLASFGFMDD965 pKa = 5.62 ARR967 pKa = 11.84 DD968 pKa = 3.83 TPANNLAAVQIIAGPAKK985 pKa = 10.22 GVLSLGGQVVANGQWITAGDD1005 pKa = 3.58 IAAGLLSYY1013 pKa = 10.37 TPAADD1018 pKa = 3.77 GTGAAYY1024 pKa = 9.73 AALTFRR1030 pKa = 11.84 VRR1032 pKa = 11.84 DD1033 pKa = 3.89 DD1034 pKa = 3.63 GGTAGGGSDD1043 pKa = 4.05 TEE1045 pKa = 4.68 TGSHH1049 pKa = 5.27 TLVIGVTGVNDD1060 pKa = 3.76 VPVGSADD1067 pKa = 3.42 IYY1069 pKa = 11.23 RR1070 pKa = 11.84 LDD1072 pKa = 4.0 EE1073 pKa = 4.89 DD1074 pKa = 4.07 SVLDD1078 pKa = 3.67 VLAPGVLANDD1088 pKa = 4.27 PDD1090 pKa = 4.51 LDD1092 pKa = 4.25 GDD1094 pKa = 3.95 MLVAEE1099 pKa = 4.95 LVSGPTNGTLVLAADD1114 pKa = 4.28 GSFRR1118 pKa = 11.84 YY1119 pKa = 7.94 TPSANWSGTEE1129 pKa = 3.96 SFSYY1133 pKa = 10.56 RR1134 pKa = 11.84 PFDD1137 pKa = 4.3 GIVYY1141 pKa = 10.48 GDD1143 pKa = 3.6 TTQVILVVEE1152 pKa = 4.63 AVNDD1156 pKa = 3.65 APEE1159 pKa = 4.87 IIHH1162 pKa = 7.12 ASFKK1166 pKa = 10.58 VPGGGVVVLNADD1178 pKa = 3.35 MMIASDD1184 pKa = 4.11 VDD1186 pKa = 3.9 SLPTSIHH1193 pKa = 5.51 FQVDD1197 pKa = 3.48 QVDD1200 pKa = 3.54 NGHH1203 pKa = 6.51 FEE1205 pKa = 4.49 LVSAPGQAIRR1215 pKa = 11.84 QFSYY1219 pKa = 11.55 AEE1221 pKa = 3.89 LAAGLVV1227 pKa = 3.63
Molecular weight: 124.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.716
IPC2_protein 3.528
IPC_protein 3.605
Toseland 3.35
ProMoST 3.783
Dawson 3.617
Bjellqvist 3.757
Wikipedia 3.579
Rodwell 3.414
Grimsley 3.249
Solomon 3.605
Lehninger 3.567
Nozaki 3.719
DTASelect 4.037
Thurlkill 3.414
EMBOSS 3.579
Sillero 3.719
Patrickios 1.507
IPC_peptide 3.605
IPC2_peptide 3.694
IPC2.peptide.svr19 3.695
Protein with the highest isoelectric point:
>tr|A0A6C2CYH3|A0A6C2CYH3_9RHOO Glutamate dehydrogenase OS=Zoogloea oleivorans OX=1552750 GN=ETQ85_10755 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 9.97 QPSVVRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.6 RR14 pKa = 11.84 THH16 pKa = 5.75 GFLVRR21 pKa = 11.84 MGTRR25 pKa = 11.84 GGRR28 pKa = 11.84 KK29 pKa = 8.93 VIAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.0 GRR39 pKa = 11.84 HH40 pKa = 4.92 RR41 pKa = 11.84 LAVV44 pKa = 3.37
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.429
IPC2_protein 11.111
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.31
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.047
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.084
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4972
0
4972
1663900
24
4131
334.7
36.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.985 ± 0.042
0.981 ± 0.012
5.494 ± 0.024
5.76 ± 0.032
3.638 ± 0.021
8.155 ± 0.044
2.247 ± 0.017
4.828 ± 0.023
3.495 ± 0.035
10.951 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.267 ± 0.019
2.768 ± 0.023
5.079 ± 0.027
3.575 ± 0.021
6.996 ± 0.04
5.61 ± 0.03
5.165 ± 0.043
7.391 ± 0.031
1.358 ± 0.015
2.256 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here