Aerococcus urinae (strain ACS-120-V-Col10a)
Average proteome isoelectric point is 5.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1684 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F2I8X0|F2I8X0_AERUA SCP-like protein OS=Aerococcus urinae (strain ACS-120-V-Col10a) OX=866775 GN=HMPREF9243_0722 PE=4 SV=1
MM1 pKa = 7.66 EE2 pKa = 5.08 FNKK5 pKa = 10.48 KK6 pKa = 10.04 LLLGLSAVGLGVFGLSDD23 pKa = 3.25 QVQADD28 pKa = 4.28 EE29 pKa = 4.6 YY30 pKa = 11.5 DD31 pKa = 4.27 DD32 pKa = 5.13 YY33 pKa = 12.19 DD34 pKa = 6.49 DD35 pKa = 5.16 IYY37 pKa = 11.45 EE38 pKa = 4.71 DD39 pKa = 4.27 YY40 pKa = 10.98 DD41 pKa = 4.1 YY42 pKa = 11.69 DD43 pKa = 6.27 DD44 pKa = 4.94 YY45 pKa = 12.13 DD46 pKa = 6.3 DD47 pKa = 5.38 YY48 pKa = 12.1 DD49 pKa = 6.03 DD50 pKa = 5.65 YY51 pKa = 12.14 DD52 pKa = 6.15 DD53 pKa = 6.6 YY54 pKa = 12.13 DD55 pKa = 5.68 DD56 pKa = 6.24 DD57 pKa = 5.06 WDD59 pKa = 5.29 DD60 pKa = 5.9 DD61 pKa = 4.39 DD62 pKa = 7.06 DD63 pKa = 5.2 IYY65 pKa = 11.76 YY66 pKa = 10.55 LSPSQNVAPAPAAYY80 pKa = 9.89 QEE82 pKa = 4.45 SKK84 pKa = 10.86 VVNTQYY90 pKa = 10.8 QVEE93 pKa = 4.31 IDD95 pKa = 4.35 DD96 pKa = 5.02 DD97 pKa = 3.9 HH98 pKa = 7.28 TDD100 pKa = 4.11 DD101 pKa = 4.12 YY102 pKa = 12.06 DD103 pKa = 3.78 EE104 pKa = 4.65 VHH106 pKa = 5.68 QQTPQVQALSDD117 pKa = 3.81 SDD119 pKa = 4.24 ALEE122 pKa = 4.2 ASLVHH127 pKa = 6.65 SGLAQAEE134 pKa = 4.42 IKK136 pKa = 10.63 DD137 pKa = 4.08 LEE139 pKa = 4.64 IEE141 pKa = 4.67 ADD143 pKa = 3.86 DD144 pKa = 3.76 EE145 pKa = 4.68 DD146 pKa = 4.11 GRR148 pKa = 11.84 LVYY151 pKa = 10.02 EE152 pKa = 4.3 VEE154 pKa = 4.03 FEE156 pKa = 4.39 YY157 pKa = 11.23 GDD159 pKa = 3.49 KK160 pKa = 10.53 EE161 pKa = 4.15 YY162 pKa = 10.73 EE163 pKa = 4.05 YY164 pKa = 11.02 LIDD167 pKa = 4.15 GLNAAVLDD175 pKa = 4.1 YY176 pKa = 11.35 EE177 pKa = 4.99 HH178 pKa = 7.72 DD179 pKa = 3.99 YY180 pKa = 11.94 DD181 pKa = 6.3 DD182 pKa = 6.7 DD183 pKa = 6.41 SDD185 pKa = 5.62 DD186 pKa = 5.74 DD187 pKa = 5.06 YY188 pKa = 12.19 DD189 pKa = 7.0 DD190 pKa = 5.61 IDD192 pKa = 5.33 DD193 pKa = 5.1 DD194 pKa = 3.88 QDD196 pKa = 3.35 EE197 pKa = 4.41 AQEE200 pKa = 4.23 YY201 pKa = 10.54 EE202 pKa = 5.02 DD203 pKa = 6.62 DD204 pKa = 4.69 IDD206 pKa = 6.26 DD207 pKa = 5.14 DD208 pKa = 4.75 LKK210 pKa = 11.33 QGLDD214 pKa = 3.51 NNLEE218 pKa = 3.92 DD219 pKa = 5.77 HH220 pKa = 7.14 YY221 pKa = 11.79 DD222 pKa = 3.96 DD223 pKa = 5.98 DD224 pKa = 6.51 LNDD227 pKa = 4.72 DD228 pKa = 4.33 FEE230 pKa = 7.17 DD231 pKa = 5.34 DD232 pKa = 3.69 NRR234 pKa = 11.84 QGQDD238 pKa = 3.33 DD239 pKa = 4.93 DD240 pKa = 5.45 LADD243 pKa = 4.88 DD244 pKa = 5.75 HH245 pKa = 7.88 DD246 pKa = 4.98 DD247 pKa = 4.19 LNNDD251 pKa = 4.83 KK252 pKa = 11.08 EE253 pKa = 4.57 DD254 pKa = 3.57 TNQAHH259 pKa = 7.12 DD260 pKa = 5.29 KK261 pKa = 10.32 KK262 pKa = 11.0 AQDD265 pKa = 3.48 QGQITPQPTGNPQSEE280 pKa = 4.42 EE281 pKa = 4.33 KK282 pKa = 10.1 PVKK285 pKa = 8.38 TKK287 pKa = 10.52 KK288 pKa = 9.09 VSKK291 pKa = 10.53 VKK293 pKa = 9.58 RR294 pKa = 11.84 TQQ296 pKa = 2.78
Molecular weight: 34.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.798
IPC2_protein 3.427
IPC_protein 3.49
Toseland 3.236
ProMoST 3.668
Dawson 3.503
Bjellqvist 3.656
Wikipedia 3.465
Rodwell 3.3
Grimsley 3.147
Solomon 3.49
Lehninger 3.452
Nozaki 3.605
DTASelect 3.91
Thurlkill 3.3
EMBOSS 3.465
Sillero 3.617
Patrickios 0.807
IPC_peptide 3.49
IPC2_peptide 3.579
IPC2.peptide.svr19 3.636
Protein with the highest isoelectric point:
>tr|F2I8V5|F2I8V5_AERUA Aminopeptidase OS=Aerococcus urinae (strain ACS-120-V-Col10a) OX=866775 GN=HMPREF9243_0702 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.3 QPNKK9 pKa = 9.14 RR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 QKK14 pKa = 10.64 KK15 pKa = 8.16 HH16 pKa = 4.35 GFRR19 pKa = 11.84 NRR21 pKa = 11.84 MSTKK25 pKa = 10.03 NGRR28 pKa = 11.84 HH29 pKa = 3.65 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 QKK37 pKa = 10.29 GRR39 pKa = 11.84 KK40 pKa = 8.74 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.45
IPC2_protein 11.169
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.427
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.149
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.074
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1684
0
1684
539492
37
3279
320.4
35.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.913 ± 0.07
0.622 ± 0.016
6.382 ± 0.063
6.843 ± 0.062
4.123 ± 0.049
6.716 ± 0.073
2.059 ± 0.025
7.133 ± 0.065
6.168 ± 0.074
9.928 ± 0.106
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.409 ± 0.034
4.479 ± 0.037
3.603 ± 0.047
5.028 ± 0.05
4.038 ± 0.045
6.128 ± 0.066
4.967 ± 0.063
6.672 ± 0.05
0.854 ± 0.019
3.934 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here