Aerococcus urinae (strain ACS-120-V-Col10a)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Aerococcaceae; Aerococcus; Aerococcus urinae

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1684 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|F2I8X0|F2I8X0_AERUA SCP-like protein OS=Aerococcus urinae (strain ACS-120-V-Col10a) OX=866775 GN=HMPREF9243_0722 PE=4 SV=1
MM1 pKa = 7.66EE2 pKa = 5.08FNKK5 pKa = 10.48KK6 pKa = 10.04LLLGLSAVGLGVFGLSDD23 pKa = 3.25QVQADD28 pKa = 4.28EE29 pKa = 4.6YY30 pKa = 11.5DD31 pKa = 4.27DD32 pKa = 5.13YY33 pKa = 12.19DD34 pKa = 6.49DD35 pKa = 5.16IYY37 pKa = 11.45EE38 pKa = 4.71DD39 pKa = 4.27YY40 pKa = 10.98DD41 pKa = 4.1YY42 pKa = 11.69DD43 pKa = 6.27DD44 pKa = 4.94YY45 pKa = 12.13DD46 pKa = 6.3DD47 pKa = 5.38YY48 pKa = 12.1DD49 pKa = 6.03DD50 pKa = 5.65YY51 pKa = 12.14DD52 pKa = 6.15DD53 pKa = 6.6YY54 pKa = 12.13DD55 pKa = 5.68DD56 pKa = 6.24DD57 pKa = 5.06WDD59 pKa = 5.29DD60 pKa = 5.9DD61 pKa = 4.39DD62 pKa = 7.06DD63 pKa = 5.2IYY65 pKa = 11.76YY66 pKa = 10.55LSPSQNVAPAPAAYY80 pKa = 9.89QEE82 pKa = 4.45SKK84 pKa = 10.86VVNTQYY90 pKa = 10.8QVEE93 pKa = 4.31IDD95 pKa = 4.35DD96 pKa = 5.02DD97 pKa = 3.9HH98 pKa = 7.28TDD100 pKa = 4.11DD101 pKa = 4.12YY102 pKa = 12.06DD103 pKa = 3.78EE104 pKa = 4.65VHH106 pKa = 5.68QQTPQVQALSDD117 pKa = 3.81SDD119 pKa = 4.24ALEE122 pKa = 4.2ASLVHH127 pKa = 6.65SGLAQAEE134 pKa = 4.42IKK136 pKa = 10.63DD137 pKa = 4.08LEE139 pKa = 4.64IEE141 pKa = 4.67ADD143 pKa = 3.86DD144 pKa = 3.76EE145 pKa = 4.68DD146 pKa = 4.11GRR148 pKa = 11.84LVYY151 pKa = 10.02EE152 pKa = 4.3VEE154 pKa = 4.03FEE156 pKa = 4.39YY157 pKa = 11.23GDD159 pKa = 3.49KK160 pKa = 10.53EE161 pKa = 4.15YY162 pKa = 10.73EE163 pKa = 4.05YY164 pKa = 11.02LIDD167 pKa = 4.15GLNAAVLDD175 pKa = 4.1YY176 pKa = 11.35EE177 pKa = 4.99HH178 pKa = 7.72DD179 pKa = 3.99YY180 pKa = 11.94DD181 pKa = 6.3DD182 pKa = 6.7DD183 pKa = 6.41SDD185 pKa = 5.62DD186 pKa = 5.74DD187 pKa = 5.06YY188 pKa = 12.19DD189 pKa = 7.0DD190 pKa = 5.61IDD192 pKa = 5.33DD193 pKa = 5.1DD194 pKa = 3.88QDD196 pKa = 3.35EE197 pKa = 4.41AQEE200 pKa = 4.23YY201 pKa = 10.54EE202 pKa = 5.02DD203 pKa = 6.62DD204 pKa = 4.69IDD206 pKa = 6.26DD207 pKa = 5.14DD208 pKa = 4.75LKK210 pKa = 11.33QGLDD214 pKa = 3.51NNLEE218 pKa = 3.92DD219 pKa = 5.77HH220 pKa = 7.14YY221 pKa = 11.79DD222 pKa = 3.96DD223 pKa = 5.98DD224 pKa = 6.51LNDD227 pKa = 4.72DD228 pKa = 4.33FEE230 pKa = 7.17DD231 pKa = 5.34DD232 pKa = 3.69NRR234 pKa = 11.84QGQDD238 pKa = 3.33DD239 pKa = 4.93DD240 pKa = 5.45LADD243 pKa = 4.88DD244 pKa = 5.75HH245 pKa = 7.88DD246 pKa = 4.98DD247 pKa = 4.19LNNDD251 pKa = 4.83KK252 pKa = 11.08EE253 pKa = 4.57DD254 pKa = 3.57TNQAHH259 pKa = 7.12DD260 pKa = 5.29KK261 pKa = 10.32KK262 pKa = 11.0AQDD265 pKa = 3.48QGQITPQPTGNPQSEE280 pKa = 4.42EE281 pKa = 4.33KK282 pKa = 10.1PVKK285 pKa = 8.38TKK287 pKa = 10.52KK288 pKa = 9.09VSKK291 pKa = 10.53VKK293 pKa = 9.58RR294 pKa = 11.84TQQ296 pKa = 2.78

Molecular weight:
34.12 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|F2I8V5|F2I8V5_AERUA Aminopeptidase OS=Aerococcus urinae (strain ACS-120-V-Col10a) OX=866775 GN=HMPREF9243_0702 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.3QPNKK9 pKa = 9.14RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84QKK14 pKa = 10.64KK15 pKa = 8.16HH16 pKa = 4.35GFRR19 pKa = 11.84NRR21 pKa = 11.84MSTKK25 pKa = 10.03NGRR28 pKa = 11.84HH29 pKa = 3.65VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.29GRR39 pKa = 11.84KK40 pKa = 8.74RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight:
5.48 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1684

0

1684

539492

37

3279

320.4

35.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.913 ± 0.07

0.622 ± 0.016

6.382 ± 0.063

6.843 ± 0.062

4.123 ± 0.049

6.716 ± 0.073

2.059 ± 0.025

7.133 ± 0.065

6.168 ± 0.074

9.928 ± 0.106

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.409 ± 0.034

4.479 ± 0.037

3.603 ± 0.047

5.028 ± 0.05

4.038 ± 0.045

6.128 ± 0.066

4.967 ± 0.063

6.672 ± 0.05

0.854 ± 0.019

3.934 ± 0.042

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski