Anaerotruncus sp. G3(2012)
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3406 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R9L9F1|R9L9F1_9FIRM Aminotransferase OS=Anaerotruncus sp. G3(2012) OX=1235835 GN=C814_03091 PE=3 SV=1
MM1 pKa = 8.21 DD2 pKa = 4.26 YY3 pKa = 11.41 EE4 pKa = 5.42 KK5 pKa = 10.13 MDD7 pKa = 3.7 SLQIDD12 pKa = 3.91 VLRR15 pKa = 11.84 EE16 pKa = 3.55 IGNIGSGNAATSLSEE31 pKa = 4.41 FLSCPVNIGVPSVQILDD48 pKa = 3.54 YY49 pKa = 10.61 DD50 pKa = 3.96 QVVEE54 pKa = 4.12 ALGGPEE60 pKa = 3.6 TMMVGLLLQLEE71 pKa = 4.37 DD72 pKa = 5.35 DD73 pKa = 3.93 IKK75 pKa = 11.36 GMIMFLLHH83 pKa = 7.12 KK84 pKa = 9.6 EE85 pKa = 4.14 FAHH88 pKa = 5.99 MALSALMGDD97 pKa = 4.51 MYY99 pKa = 11.58 NSFDD103 pKa = 3.76 EE104 pKa = 4.13 MDD106 pKa = 4.89 EE107 pKa = 4.09 ISASAMQEE115 pKa = 3.74 VGNIMAASYY124 pKa = 11.17 VNAIADD130 pKa = 3.74 MTGLSINISVPSICMDD146 pKa = 3.25 MAGAIVSVPAVYY158 pKa = 10.53 YY159 pKa = 11.12 ADD161 pKa = 3.93 ISDD164 pKa = 4.3 KK165 pKa = 10.84 IIFIGDD171 pKa = 3.36 DD172 pKa = 3.62 FVRR175 pKa = 11.84 DD176 pKa = 4.24 DD177 pKa = 3.75 SHH179 pKa = 7.31 HH180 pKa = 6.13 AANFNSHH187 pKa = 5.99 ILMIPEE193 pKa = 4.18 SDD195 pKa = 3.61 SLEE198 pKa = 4.84 KK199 pKa = 10.77 IMTSLGIEE207 pKa = 3.91 II208 pKa = 4.75
Molecular weight: 22.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.742
IPC2_protein 3.859
IPC_protein 3.846
Toseland 3.63
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.77
Rodwell 3.681
Grimsley 3.541
Solomon 3.834
Lehninger 3.783
Nozaki 3.948
DTASelect 4.177
Thurlkill 3.694
EMBOSS 3.783
Sillero 3.973
Patrickios 1.901
IPC_peptide 3.821
IPC2_peptide 3.948
IPC2.peptide.svr19 3.852
Protein with the highest isoelectric point:
>tr|R9LAJ6|R9LAJ6_9FIRM Uncharacterized protein (Fragment) OS=Anaerotruncus sp. G3(2012) OX=1235835 GN=C814_03085 PE=4 SV=1
MM1 pKa = 7.32 RR2 pKa = 11.84 RR3 pKa = 11.84 VYY5 pKa = 10.06 PVSLCFFPALVHH17 pKa = 6.87 FFTKK21 pKa = 10.65 DD22 pKa = 3.27 LVVPSASNRR31 pKa = 11.84 LGSEE35 pKa = 4.23 SSTHH39 pKa = 6.6 AYY41 pKa = 9.39 LKK43 pKa = 10.44 KK44 pKa = 10.21 GCLAAAHH51 pKa = 6.69 IFCGNKK57 pKa = 9.85 APEE60 pKa = 3.91 VWIPGCGAFWVPDD73 pKa = 3.32 QNAPTRR79 pKa = 11.84 GFLAQARR86 pKa = 11.84 SAAKK90 pKa = 9.8 AATAARR96 pKa = 11.84 RR97 pKa = 11.84 TPQEE101 pKa = 3.71 GLRR104 pKa = 11.84 ATEE107 pKa = 3.85 VWIPDD112 pKa = 3.62 QTSGAQAPFFGGLLGPAARR131 pKa = 11.84 RR132 pKa = 11.84 ALRR135 pKa = 11.84 ATLGQDD141 pKa = 3.07 APHH144 pKa = 6.26 GRR146 pKa = 11.84 GVKK149 pKa = 10.37 GSIRR153 pKa = 11.84 LAPDD157 pKa = 2.8 QTDD160 pKa = 3.01 RR161 pKa = 11.84 RR162 pKa = 11.84 RR163 pKa = 11.84 APSSGLLGG171 pKa = 3.77
Molecular weight: 18.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.575
IPC_protein 10.482
Toseland 10.804
ProMoST 10.584
Dawson 10.862
Bjellqvist 10.613
Wikipedia 11.111
Rodwell 10.994
Grimsley 10.906
Solomon 11.052
Lehninger 11.008
Nozaki 10.818
DTASelect 10.613
Thurlkill 10.804
EMBOSS 11.228
Sillero 10.818
Patrickios 10.745
IPC_peptide 11.067
IPC2_peptide 9.926
IPC2.peptide.svr19 8.687
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3406
0
3406
991676
22
2436
291.2
32.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.052 ± 0.062
1.611 ± 0.021
5.416 ± 0.035
6.916 ± 0.041
3.971 ± 0.03
7.682 ± 0.045
1.757 ± 0.021
6.03 ± 0.038
4.959 ± 0.043
9.735 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.774 ± 0.023
3.489 ± 0.027
4.269 ± 0.029
3.853 ± 0.029
5.794 ± 0.041
6.035 ± 0.038
5.251 ± 0.045
6.947 ± 0.034
0.975 ± 0.014
3.483 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here