Ligilactobacillus animalis
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1844 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A062XBZ3|A0A062XBZ3_9LACO Ribosomal protein L11 methyltransferase OS=Ligilactobacillus animalis OX=1605 GN=prmA PE=3 SV=1
MM1 pKa = 7.73 YY2 pKa = 7.96 DD3 pKa = 3.07 TFLTGIYY10 pKa = 8.52 TKK12 pKa = 10.46 LRR14 pKa = 11.84 SAIEE18 pKa = 4.11 DD19 pKa = 3.98 KK20 pKa = 11.08 YY21 pKa = 11.67 DD22 pKa = 3.43 CQAWLDD28 pKa = 3.95 YY29 pKa = 10.57 IEE31 pKa = 5.66 KK32 pKa = 10.71 YY33 pKa = 10.84 GLLEE37 pKa = 3.8 QLEE40 pKa = 4.32 EE41 pKa = 4.28 SVLEE45 pKa = 4.23 IEE47 pKa = 5.43 FEE49 pKa = 4.1
Molecular weight: 5.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.854
IPC2_protein 4.088
IPC_protein 3.923
Toseland 3.77
ProMoST 3.999
Dawson 3.884
Bjellqvist 4.139
Wikipedia 3.783
Rodwell 3.77
Grimsley 3.681
Solomon 3.859
Lehninger 3.808
Nozaki 4.012
DTASelect 4.113
Thurlkill 3.808
EMBOSS 3.795
Sillero 4.037
Patrickios 0.693
IPC_peptide 3.859
IPC2_peptide 4.024
IPC2.peptide.svr19 3.998
Protein with the highest isoelectric point:
>tr|A0A062X7Z2|A0A062X7Z2_9LACO Ectoine/hydroxyectoine ABC transporter permease protein EhuD ehuD OS=Ligilactobacillus animalis OX=1605 GN=Lani381_1657 PE=3 SV=1
MM1 pKa = 7.92 PKK3 pKa = 10.14 QKK5 pKa = 7.59 THH7 pKa = 6.46 RR8 pKa = 11.84 ASAKK12 pKa = 9.12 RR13 pKa = 11.84 FKK15 pKa = 10.26 RR16 pKa = 11.84 TGNGGLKK23 pKa = 9.53 RR24 pKa = 11.84 AHH26 pKa = 7.01 AFTSHH31 pKa = 6.77 RR32 pKa = 11.84 FHH34 pKa = 7.48 GKK36 pKa = 6.37 TKK38 pKa = 9.57 KK39 pKa = 9.43 QRR41 pKa = 11.84 RR42 pKa = 11.84 QLRR45 pKa = 11.84 KK46 pKa = 8.04 PAMVSASDD54 pKa = 3.54 MKK56 pKa = 10.58 RR57 pKa = 11.84 IKK59 pKa = 10.73 QMLSQMKK66 pKa = 9.93
Molecular weight: 7.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.386
IPC2_protein 10.847
IPC_protein 12.384
Toseland 12.574
ProMoST 13.042
Dawson 12.574
Bjellqvist 12.559
Wikipedia 13.042
Rodwell 12.471
Grimsley 12.618
Solomon 13.056
Lehninger 12.954
Nozaki 12.574
DTASelect 12.559
Thurlkill 12.574
EMBOSS 13.056
Sillero 12.574
Patrickios 12.193
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.005
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1844
0
1844
542867
29
1959
294.4
32.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.318 ± 0.061
0.631 ± 0.017
5.663 ± 0.06
6.502 ± 0.063
4.261 ± 0.056
6.55 ± 0.056
1.81 ± 0.023
6.463 ± 0.063
7.322 ± 0.055
10.436 ± 0.093
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.484 ± 0.028
4.362 ± 0.039
3.598 ± 0.037
4.404 ± 0.051
3.893 ± 0.043
5.393 ± 0.056
6.054 ± 0.062
7.119 ± 0.06
0.959 ± 0.023
3.776 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here