Kurthia sp. 11kri321

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Planococcaceae; Kurthia; unclassified Kurthia

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2893 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0X1S0Q1|A0A0X1S0Q1_9BACL Pseudouridine synthase OS=Kurthia sp. 11kri321 OX=1750719 GN=rluB PE=3 SV=1
MM1 pKa = 7.68EE2 pKa = 4.82IVIPEE7 pKa = 3.78QDD9 pKa = 4.11VINSICLYY17 pKa = 9.83HH18 pKa = 7.9ARR20 pKa = 11.84EE21 pKa = 4.04KK22 pKa = 11.17NIIPEE27 pKa = 4.14DD28 pKa = 3.82VEE30 pKa = 4.37VEE32 pKa = 4.13LMYY35 pKa = 11.08DD36 pKa = 3.52DD37 pKa = 4.71EE38 pKa = 6.27LGFEE42 pKa = 5.04AEE44 pKa = 4.23AFSNGMSTLYY54 pKa = 10.75KK55 pKa = 9.05EE56 pKa = 4.9GNMISALRR64 pKa = 11.84VWVEE68 pKa = 4.79DD69 pKa = 3.87YY70 pKa = 11.79LNMNPFSTSIRR81 pKa = 11.84LEE83 pKa = 4.11LDD85 pKa = 3.32DD86 pKa = 4.12EE87 pKa = 4.41QGIIAYY93 pKa = 9.63AAEE96 pKa = 4.07

Molecular weight: 11.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A109QDV7|A0A109QDV7_9BACL O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase family protein OS=Kurthia sp. 11kri321 OX=1750719 GN=ASO14_2684 PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.54QPKK8 pKa = 9.44KK9 pKa = 7.96RR10 pKa = 11.84KK11 pKa = 8.69HH12 pKa = 5.94SKK14 pKa = 8.54VHH16 pKa = 5.68GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSKK25 pKa = 9.98NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2893

0

2893

849731

29

1583

293.7

33.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.598 ± 0.051

0.69 ± 0.013

5.024 ± 0.038

7.534 ± 0.058

4.443 ± 0.04

6.392 ± 0.048

2.178 ± 0.024

7.725 ± 0.048

6.862 ± 0.051

9.563 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.862 ± 0.018

4.014 ± 0.031

3.439 ± 0.024

4.452 ± 0.04

4.011 ± 0.031

5.455 ± 0.035

5.951 ± 0.032

7.255 ± 0.039

0.896 ± 0.016

3.658 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski