Fengkai orbivirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Orbivirus; unclassified Orbivirus; Tibet orbivirus

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K1TPZ3|A0A0K1TPZ3_9REOV Non-structural protein NS1 OS=Fengkai orbivirus OX=1692107 GN=NS1 PE=4 SV=1
MM1 pKa = 6.78EE2 pKa = 5.71HH3 pKa = 6.36KK4 pKa = 10.33PKK6 pKa = 10.73RR7 pKa = 11.84FTKK10 pKa = 10.31NVSVLDD16 pKa = 4.06GSGTTICGIIAKK28 pKa = 9.39SAHH31 pKa = 6.37KK32 pKa = 9.26PYY34 pKa = 10.79CNISIGRR41 pKa = 11.84NVSIKK46 pKa = 10.69AVDD49 pKa = 3.74NPPPKK54 pKa = 10.44AYY56 pKa = 10.33VLQIRR61 pKa = 11.84QEE63 pKa = 3.84GGYY66 pKa = 9.99RR67 pKa = 11.84IQDD70 pKa = 3.65GQDD73 pKa = 3.45TISLMITASGVEE85 pKa = 3.99ATVEE89 pKa = 4.14RR90 pKa = 11.84WEE92 pKa = 4.0EE93 pKa = 3.74WQFEE97 pKa = 4.57VLSAMPMPMAIMMQHH112 pKa = 6.01NGEE115 pKa = 4.19MVDD118 pKa = 5.2AEE120 pKa = 4.11IKK122 pKa = 8.51YY123 pKa = 9.55AKK125 pKa = 10.36GMGVVAPYY133 pKa = 10.46VKK135 pKa = 10.37NDD137 pKa = 3.08EE138 pKa = 4.07VDD140 pKa = 3.37RR141 pKa = 11.84RR142 pKa = 11.84EE143 pKa = 4.12LPKK146 pKa = 10.9LPGLSEE152 pKa = 3.85SQYY155 pKa = 11.24DD156 pKa = 3.91VKK158 pKa = 10.15TLRR161 pKa = 11.84QKK163 pKa = 10.44IKK165 pKa = 9.53EE166 pKa = 3.94EE167 pKa = 3.97RR168 pKa = 11.84EE169 pKa = 3.86RR170 pKa = 11.84GVTEE174 pKa = 3.82IRR176 pKa = 11.84SKK178 pKa = 10.4IQPLEE183 pKa = 3.99QEE185 pKa = 4.52TKK187 pKa = 10.59RR188 pKa = 11.84MEE190 pKa = 4.27SVEE193 pKa = 4.1KK194 pKa = 10.93SFAEE198 pKa = 4.37MKK200 pKa = 10.54AIWAAEE206 pKa = 3.99SKK208 pKa = 11.29ADD210 pKa = 3.7VKK212 pKa = 11.05EE213 pKa = 4.17EE214 pKa = 4.24NVGGTYY220 pKa = 9.34GTDD223 pKa = 3.49FFKK226 pKa = 10.82PFPIEE231 pKa = 4.01STKK234 pKa = 10.2MPEE237 pKa = 4.23LKK239 pKa = 10.5SLEE242 pKa = 3.75QRR244 pKa = 11.84EE245 pKa = 4.12QQKK248 pKa = 10.43EE249 pKa = 4.21KK250 pKa = 10.77EE251 pKa = 4.33VAQAQSFSVDD261 pKa = 3.26VEE263 pKa = 4.48SGSEE267 pKa = 4.13EE268 pKa = 4.03EE269 pKa = 4.39VEE271 pKa = 4.09EE272 pKa = 4.21PNYY275 pKa = 9.03ITQEE279 pKa = 4.21YY280 pKa = 9.35VDD282 pKa = 4.1KK283 pKa = 10.92ASKK286 pKa = 9.56VVKK289 pKa = 10.5GGEE292 pKa = 3.99KK293 pKa = 10.06KK294 pKa = 10.15IAGLAYY300 pKa = 10.63VMPKK304 pKa = 10.46SIGDD308 pKa = 3.52FSKK311 pKa = 10.45IIAVKK316 pKa = 8.67KK317 pKa = 9.6HH318 pKa = 5.14KK319 pKa = 9.47WSNVPLFTVDD329 pKa = 3.59EE330 pKa = 4.72KK331 pKa = 11.29GLKK334 pKa = 10.16YY335 pKa = 10.04EE336 pKa = 4.07LQTIGEE342 pKa = 4.74CEE344 pKa = 4.18TVVYY348 pKa = 10.02AARR351 pKa = 11.84GLNLLVLPVGII362 pKa = 4.83

Molecular weight: 40.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K1TPZ7|A0A0K1TPZ7_9REOV Non-structural protein NS3 OS=Fengkai orbivirus OX=1692107 GN=NS3 PE=3 SV=1
MM1 pKa = 7.72LSGLVRR7 pKa = 11.84RR8 pKa = 11.84FEE10 pKa = 4.15EE11 pKa = 4.26EE12 pKa = 4.12RR13 pKa = 11.84IEE15 pKa = 4.07MPQEE19 pKa = 3.92VKK21 pKa = 10.37PDD23 pKa = 3.52EE24 pKa = 4.57LSLVNYY30 pKa = 9.01YY31 pKa = 9.98PSEE34 pKa = 4.16SRR36 pKa = 11.84PPSYY40 pKa = 11.46APMAPTMTDD49 pKa = 2.4GMANVSLGILNQAMTNTTGATTASKK74 pKa = 10.72LEE76 pKa = 3.83KK77 pKa = 10.04AAYY80 pKa = 9.67GSYY83 pKa = 11.22AEE85 pKa = 4.89AFRR88 pKa = 11.84DD89 pKa = 3.9DD90 pKa = 3.47LRR92 pKa = 11.84LRR94 pKa = 11.84QVKK97 pKa = 9.85KK98 pKa = 10.12HH99 pKa = 6.07VNDD102 pKa = 3.67QIIPRR107 pKa = 11.84LSTEE111 pKa = 3.89LSHH114 pKa = 7.45LKK116 pKa = 9.9RR117 pKa = 11.84KK118 pKa = 9.95KK119 pKa = 10.35IIVGTIMLVAAIVALVTSAGTLTNDD144 pKa = 4.43LSISMKK150 pKa = 10.75AGDD153 pKa = 3.98VNITIPAWFKK163 pKa = 10.84GFSAVFGFANLGSTMIMMLCARR185 pKa = 11.84TEE187 pKa = 3.87RR188 pKa = 11.84MLASQIEE195 pKa = 4.58MIQKK199 pKa = 9.96EE200 pKa = 4.33LMKK203 pKa = 10.53KK204 pKa = 10.1RR205 pKa = 11.84SYY207 pKa = 11.28NEE209 pKa = 3.65AVRR212 pKa = 11.84MSTAEE217 pKa = 3.85LSGMSLEE224 pKa = 4.94GIIPNTGQLTT234 pKa = 3.68

Molecular weight: 25.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

6167

234

1304

616.7

70.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.297 ± 0.529

1.005 ± 0.298

5.919 ± 0.333

7.508 ± 0.752

3.616 ± 0.339

5.951 ± 0.363

2.416 ± 0.222

6.713 ± 0.445

6.535 ± 0.899

8.659 ± 0.455

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.892 ± 0.242

3.357 ± 0.206

3.859 ± 0.361

3.746 ± 0.299

6.502 ± 0.418

5.643 ± 0.419

5.254 ± 0.385

6.94 ± 0.349

1.297 ± 0.234

3.892 ± 0.383
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski