Sphingorhabdus soli
Average proteome isoelectric point is 6.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2468 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C6UN05|A0A5C6UN05_9SPHN DNA gyrase inhibitor YacG OS=Sphingorhabdus soli OX=2601267 GN=yacG PE=3 SV=1
MM1 pKa = 6.69 PTIYY5 pKa = 9.79 VTDD8 pKa = 3.59 RR9 pKa = 11.84 AGNEE13 pKa = 3.82 RR14 pKa = 11.84 AVEE17 pKa = 4.02 AKK19 pKa = 9.82 PGYY22 pKa = 10.39 SVMEE26 pKa = 4.63 TIRR29 pKa = 11.84 DD30 pKa = 3.51 AGFDD34 pKa = 4.3 DD35 pKa = 6.0 LLALCGGCCSCATCHH50 pKa = 5.99 VVVDD54 pKa = 4.43 DD55 pKa = 4.79 AQLSLLPAMSEE66 pKa = 4.86 DD67 pKa = 4.28 EE68 pKa = 5.69 DD69 pKa = 5.04 DD70 pKa = 5.46 LLDD73 pKa = 4.51 SSDD76 pKa = 3.26 HH77 pKa = 5.61 RR78 pKa = 11.84 TAGSRR83 pKa = 11.84 LSCQIDD89 pKa = 3.5 VSDD92 pKa = 4.81 ALDD95 pKa = 3.82 GLKK98 pKa = 8.98 VTIAPEE104 pKa = 3.91 DD105 pKa = 3.37
Molecular weight: 11.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.747
IPC2_protein 3.91
IPC_protein 3.884
Toseland 3.668
ProMoST 4.012
Dawson 3.897
Bjellqvist 4.088
Wikipedia 3.859
Rodwell 3.719
Grimsley 3.579
Solomon 3.884
Lehninger 3.846
Nozaki 4.012
DTASelect 4.279
Thurlkill 3.732
EMBOSS 3.859
Sillero 4.012
Patrickios 1.926
IPC_peptide 3.872
IPC2_peptide 3.986
IPC2.peptide.svr19 3.932
Protein with the highest isoelectric point:
>tr|A0A5C6U973|A0A5C6U973_9SPHN Recombinase family protein OS=Sphingorhabdus soli OX=2601267 GN=FSZ31_11895 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.89 GFFARR21 pKa = 11.84 KK22 pKa = 7.44 ATPGGRR28 pKa = 11.84 NILRR32 pKa = 11.84 ARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.02 KK41 pKa = 10.61 LSAA44 pKa = 4.03
Molecular weight: 5.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.647
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.51
Sillero 12.998
Patrickios 12.369
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.185
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2468
0
2468
779872
31
1584
316.0
34.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.591 ± 0.077
0.781 ± 0.014
6.576 ± 0.042
5.223 ± 0.05
3.45 ± 0.034
8.846 ± 0.052
1.952 ± 0.024
5.423 ± 0.033
3.184 ± 0.041
9.525 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.416 ± 0.023
2.539 ± 0.03
5.13 ± 0.034
2.887 ± 0.028
7.147 ± 0.053
5.352 ± 0.041
5.323 ± 0.036
7.113 ± 0.038
1.34 ± 0.02
2.202 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here