Polymorphum gilvum (strain LMG 25793 / CGMCC 1.9160 / SL003B-26A1)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Polymorphum; Polymorphum gilvum

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4354 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F2J431|F2J431_POLGS Uncharacterized protein OS=Polymorphum gilvum (strain LMG 25793 / CGMCC 1.9160 / SL003B-26A1) OX=991905 GN=SL003B_1529 PE=4 SV=1
MM1 pKa = 6.54TQADD5 pKa = 4.1AVIDD9 pKa = 4.23GQTTFATTEE18 pKa = 4.15YY19 pKa = 8.68YY20 pKa = 9.98TRR22 pKa = 11.84AGDD25 pKa = 3.99FEE27 pKa = 5.68LNEE30 pKa = 4.65DD31 pKa = 3.94GYY33 pKa = 10.13MVNSAGYY40 pKa = 8.9YY41 pKa = 10.79LMGFPLDD48 pKa = 3.62ARR50 pKa = 11.84TGNAVGDD57 pKa = 4.05NPEE60 pKa = 4.26VIQIGSQPLPAQATSEE76 pKa = 3.99IKK78 pKa = 10.68YY79 pKa = 9.51QANLPSYY86 pKa = 9.1PQTNNADD93 pKa = 3.5TSVPGSQYY101 pKa = 11.09LDD103 pKa = 3.13TSGAITTTSDD113 pKa = 2.87IAVADD118 pKa = 3.44IDD120 pKa = 5.08DD121 pKa = 4.64FLNSSISGQSITVYY135 pKa = 10.23DD136 pKa = 3.95QNGTPLNVEE145 pKa = 4.12VRR147 pKa = 11.84WAQTAANTWNAYY159 pKa = 9.19YY160 pKa = 10.68SSGGDD165 pKa = 3.85AVTGWYY171 pKa = 10.46LIGEE175 pKa = 4.54VTFDD179 pKa = 3.25AAGVMDD185 pKa = 4.89TLTPAGTNTVGAGGASFVIDD205 pKa = 4.33DD206 pKa = 4.07LTVAGVNAGDD216 pKa = 3.92IEE218 pKa = 4.54FSFKK222 pKa = 10.47KK223 pKa = 10.77GSLTQYY229 pKa = 11.15ADD231 pKa = 3.56PKK233 pKa = 10.41GLASSVSLNQDD244 pKa = 2.8GYY246 pKa = 11.71AAGQVVGLSIDD257 pKa = 3.38GAGRR261 pKa = 11.84IIASYY266 pKa = 11.02SNQQTRR272 pKa = 11.84SLFEE276 pKa = 4.12IPLATFQADD285 pKa = 3.38QNLRR289 pKa = 11.84RR290 pKa = 11.84VDD292 pKa = 3.78GSAFAATPSSGKK304 pKa = 9.9AAYY307 pKa = 10.01GNGGTIVANALEE319 pKa = 4.44SSNADD324 pKa = 2.88IAAEE328 pKa = 3.99FSKK331 pKa = 11.23LIITQQAYY339 pKa = 9.4SANSKK344 pKa = 10.15VVTSADD350 pKa = 3.24QMLNDD355 pKa = 4.43ALNMVRR361 pKa = 5.43

Molecular weight: 37.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F2J217|F2J217_POLGS von Willebrand factor type A:Cobalt chelatase pCobT subunit OS=Polymorphum gilvum (strain LMG 25793 / CGMCC 1.9160 / SL003B-26A1) OX=991905 GN=SL003B_0341 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.2QPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 9.21RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.48GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.29NGRR28 pKa = 11.84QVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.99GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4354

0

4354

1377863

39

2444

316.5

34.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.342 ± 0.055

0.843 ± 0.011

5.997 ± 0.029

5.77 ± 0.031

3.647 ± 0.024

8.705 ± 0.042

1.979 ± 0.017

4.849 ± 0.027

2.836 ± 0.026

10.374 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.369 ± 0.018

2.221 ± 0.018

5.173 ± 0.028

2.829 ± 0.019

7.747 ± 0.045

4.966 ± 0.022

5.261 ± 0.025

7.68 ± 0.032

1.281 ± 0.014

2.131 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski