Canine adenovirus 1
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q96693|Q96693_9ADEN ORF30 OS=Canine adenovirus 1 OX=10512 GN=orf30 PE=4 SV=1
MM1 pKa = 7.47 AALGVSMGACFLLRR15 pKa = 11.84 VHH17 pKa = 6.9 KK18 pKa = 10.72 SLVEE22 pKa = 4.11 SVCAHH27 pKa = 7.04 LEE29 pKa = 4.08 LTDD32 pKa = 4.01 FLPTEE37 pKa = 4.04 LRR39 pKa = 11.84 HH40 pKa = 5.41 VLFRR44 pKa = 11.84 TIRR47 pKa = 11.84 AQEE50 pKa = 3.9 LRR52 pKa = 11.84 PWPTFAAAVSALDD65 pKa = 3.38 GDD67 pKa = 4.11 YY68 pKa = 11.18 LGFYY72 pKa = 10.13 IFIGSDD78 pKa = 3.33 TEE80 pKa = 4.62 GFGSVADD87 pKa = 4.37 LQATAADD94 pKa = 3.62 LQEE97 pKa = 4.36 AFRR100 pKa = 11.84 HH101 pKa = 6.17 DD102 pKa = 4.48 FGQLIPEE109 pKa = 4.67 DD110 pKa = 4.24 CAHH113 pKa = 7.02 LALGLSVTWEE123 pKa = 4.02 PNLLVTTEE131 pKa = 3.8
Molecular weight: 14.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.544
IPC2_protein 4.635
IPC_protein 4.533
Toseland 4.406
ProMoST 4.571
Dawson 4.495
Bjellqvist 4.698
Wikipedia 4.38
Rodwell 4.393
Grimsley 4.317
Solomon 4.495
Lehninger 4.444
Nozaki 4.609
DTASelect 4.774
Thurlkill 4.418
EMBOSS 4.406
Sillero 4.673
Patrickios 3.503
IPC_peptide 4.495
IPC2_peptide 4.66
IPC2.peptide.svr19 4.576
Protein with the highest isoelectric point:
>tr|Q65964|Q65964_9ADEN E4 orf3 OS=Canine adenovirus 1 OX=10512 GN=orf28 PE=4 SV=1
MM1 pKa = 7.64 ALVLVNGKK9 pKa = 10.18 LSGEE13 pKa = 4.18 TTLSFKK19 pKa = 10.57 HH20 pKa = 5.6 LRR22 pKa = 11.84 KK23 pKa = 9.41 LARR26 pKa = 11.84 THH28 pKa = 4.58 KK29 pKa = 9.46 WKK31 pKa = 9.78 YY32 pKa = 9.25 QSWEE36 pKa = 3.6 EE37 pKa = 3.97 GALVDD42 pKa = 6.01 IEE44 pKa = 4.46 TQDD47 pKa = 3.74 PEE49 pKa = 4.47 VLTKK53 pKa = 10.19 IRR55 pKa = 3.89
Molecular weight: 6.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.608
IPC2_protein 8.521
IPC_protein 8.653
Toseland 9.545
ProMoST 9.399
Dawson 9.663
Bjellqvist 9.224
Wikipedia 9.765
Rodwell 10.204
Grimsley 9.692
Solomon 9.823
Lehninger 9.809
Nozaki 9.443
DTASelect 9.253
Thurlkill 9.516
EMBOSS 9.897
Sillero 9.56
Patrickios 10.248
IPC_peptide 9.809
IPC2_peptide 7.746
IPC2.peptide.svr19 7.516
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
1740
55
977
217.5
24.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.839 ± 0.922
2.299 ± 0.42
5.0 ± 0.319
6.897 ± 0.809
5.0 ± 0.601
4.598 ± 0.473
2.644 ± 0.395
4.655 ± 0.616
6.034 ± 0.872
9.77 ± 1.107
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.471 ± 0.286
4.253 ± 0.835
4.828 ± 0.893
3.506 ± 0.377
4.943 ± 0.952
7.529 ± 1.193
6.724 ± 0.646
6.264 ± 0.97
1.609 ± 0.284
4.138 ± 0.995
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here