Avon-Heathcote Estuary associated circular virus 3

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 8.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5IM76|A0A0C5IM76_9CIRC Putative capsid protein OS=Avon-Heathcote Estuary associated circular virus 3 OX=1618254 PE=4 SV=1
MM1 pKa = 6.69NQARR5 pKa = 11.84FWILTIRR12 pKa = 11.84HH13 pKa = 6.39ADD15 pKa = 3.71FLPYY19 pKa = 10.48LPPTVDD25 pKa = 3.3YY26 pKa = 11.34LRR28 pKa = 11.84GQLEE32 pKa = 4.07RR33 pKa = 11.84GDD35 pKa = 4.06GGFLHH40 pKa = 6.48WQLVVHH46 pKa = 6.65FARR49 pKa = 11.84KK50 pKa = 9.04VRR52 pKa = 11.84LGGVKK57 pKa = 10.46GIFGDD62 pKa = 3.95STHH65 pKa = 7.16AEE67 pKa = 4.19PTRR70 pKa = 11.84SDD72 pKa = 3.15AARR75 pKa = 11.84EE76 pKa = 4.1YY77 pKa = 10.4VWKK80 pKa = 10.24EE81 pKa = 3.51DD82 pKa = 3.4SAVPNTRR89 pKa = 11.84FEE91 pKa = 4.36LGKK94 pKa = 9.74IPVRR98 pKa = 11.84RR99 pKa = 11.84GVSHH103 pKa = 7.2DD104 pKa = 2.91WDD106 pKa = 3.68AVRR109 pKa = 11.84EE110 pKa = 4.19SAKK113 pKa = 10.31RR114 pKa = 11.84GRR116 pKa = 11.84LDD118 pKa = 5.85DD119 pKa = 3.99IPADD123 pKa = 3.87IYY125 pKa = 11.23CRR127 pKa = 11.84LYY129 pKa = 11.48GNFKK133 pKa = 10.52RR134 pKa = 11.84IAVDD138 pKa = 3.03HH139 pKa = 5.89MVPLGIEE146 pKa = 4.06RR147 pKa = 11.84EE148 pKa = 4.34VNVYY152 pKa = 9.44WGVTGSGKK160 pKa = 8.79SRR162 pKa = 11.84RR163 pKa = 11.84AWNEE167 pKa = 3.19AGLDD171 pKa = 3.85AFPKK175 pKa = 10.49DD176 pKa = 3.69PRR178 pKa = 11.84TKK180 pKa = 10.17FWDD183 pKa = 4.42GYY185 pKa = 9.57RR186 pKa = 11.84GHH188 pKa = 6.83EE189 pKa = 3.85NVVIDD194 pKa = 4.25EE195 pKa = 4.26FRR197 pKa = 11.84GGIDD201 pKa = 3.25VAHH204 pKa = 7.59LLRR207 pKa = 11.84WFDD210 pKa = 3.74RR211 pKa = 11.84YY212 pKa = 10.05PVVVEE217 pKa = 4.03VKK219 pKa = 10.13GSSVVLSAKK228 pKa = 10.15HH229 pKa = 5.47IWITSNLDD237 pKa = 3.12PRR239 pKa = 11.84EE240 pKa = 4.04WYY242 pKa = 10.5ADD244 pKa = 3.54LDD246 pKa = 4.21AEE248 pKa = 4.38TLAALLRR255 pKa = 11.84RR256 pKa = 11.84LKK258 pKa = 9.24ITQFAII264 pKa = 3.58

Molecular weight: 30.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5IM76|A0A0C5IM76_9CIRC Putative capsid protein OS=Avon-Heathcote Estuary associated circular virus 3 OX=1618254 PE=4 SV=1
MM1 pKa = 7.28AGKK4 pKa = 10.09RR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84SFSGNVRR14 pKa = 11.84SVTRR18 pKa = 11.84VIPGPYY24 pKa = 8.91TPRR27 pKa = 11.84TTVGRR32 pKa = 11.84RR33 pKa = 11.84PATGFISGAASVARR47 pKa = 11.84QLAPQFARR55 pKa = 11.84IAAEE59 pKa = 3.95AATQTAINYY68 pKa = 6.36VTGGTQTKK76 pKa = 9.23TKK78 pKa = 9.4TKK80 pKa = 10.32SKK82 pKa = 9.84NKK84 pKa = 10.02SSTGMNVRR92 pKa = 11.84TSMSGGFFGNKK103 pKa = 5.8QKK105 pKa = 11.35KK106 pKa = 10.29NIFQKK111 pKa = 8.61YY112 pKa = 3.68TTRR115 pKa = 11.84GIVTTKK121 pKa = 8.62EE122 pKa = 3.74TANILIGGPTDD133 pKa = 3.64PDD135 pKa = 3.55LPVFFGLATHH145 pKa = 7.27ANFLLIGRR153 pKa = 11.84LFFRR157 pKa = 11.84TVVKK161 pKa = 10.69DD162 pKa = 3.49VMRR165 pKa = 11.84KK166 pKa = 9.51SGMNVSSMSDD176 pKa = 3.34TPQGGIFQLQWQIRR190 pKa = 11.84DD191 pKa = 3.17VDD193 pKa = 4.04DD194 pKa = 4.63AQSSGASVNTAGKK207 pKa = 7.38TLEE210 pKa = 4.33VIADD214 pKa = 3.95EE215 pKa = 5.54LYY217 pKa = 11.09ADD219 pKa = 3.72YY220 pKa = 11.39LSFFRR225 pKa = 11.84STLNAMEE232 pKa = 4.65LAYY235 pKa = 9.83ISSAQFWTPSFGTLLAQIDD254 pKa = 4.1LKK256 pKa = 10.69DD257 pKa = 3.6ARR259 pKa = 11.84IHH261 pKa = 6.03ILCKK265 pKa = 10.12SDD267 pKa = 5.09LKK269 pKa = 11.05AQNRR273 pKa = 11.84TLANGADD280 pKa = 4.02DD281 pKa = 5.81DD282 pKa = 4.98NNTTEE287 pKa = 4.7NIEE290 pKa = 3.94NQPLYY295 pKa = 10.5GKK297 pKa = 10.04QYY299 pKa = 8.75FGKK302 pKa = 10.6GVGLVTRR309 pKa = 11.84IGLNTTNASEE319 pKa = 4.39GKK321 pKa = 10.22SLICSQQTGLLSFAVKK337 pKa = 9.0KK338 pKa = 9.39TGTPGVNQEE347 pKa = 3.31PWLRR351 pKa = 11.84EE352 pKa = 3.84VPLPTLFCGKK362 pKa = 9.62IAYY365 pKa = 8.85GKK367 pKa = 10.56VSIEE371 pKa = 3.76PGQVKK376 pKa = 9.51TSSLTHH382 pKa = 6.34KK383 pKa = 10.83ASIKK387 pKa = 10.29QQDD390 pKa = 3.14FWNYY394 pKa = 9.5LRR396 pKa = 11.84EE397 pKa = 4.12NRR399 pKa = 11.84STAVKK404 pKa = 10.14GGRR407 pKa = 11.84PEE409 pKa = 3.42KK410 pKa = 10.15MGIFRR415 pKa = 11.84IFCVEE420 pKa = 4.71KK421 pKa = 10.07MICCTAEE428 pKa = 3.65DD429 pKa = 4.22RR430 pKa = 11.84APQLGIEE437 pKa = 4.2LNQRR441 pKa = 11.84IGLYY445 pKa = 9.29VAYY448 pKa = 9.26PRR450 pKa = 11.84VKK452 pKa = 9.57NTMEE456 pKa = 4.04MYY458 pKa = 10.46DD459 pKa = 3.38GHH461 pKa = 7.03EE462 pKa = 4.24YY463 pKa = 9.59TT464 pKa = 5.4

Molecular weight: 50.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

728

264

464

364.0

40.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.83 ± 0.068

0.962 ± 0.315

5.357 ± 1.405

4.396 ± 0.491

4.67 ± 0.068

8.791 ± 0.248

1.786 ± 0.878

5.632 ± 0.178

5.907 ± 0.736

7.555 ± 0.421

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.786 ± 0.556

4.258 ± 0.869

4.258 ± 0.155

3.846 ± 1.261

7.555 ± 1.446

5.907 ± 0.941

7.418 ± 1.964

7.005 ± 1.128

1.923 ± 1.009

3.159 ± 0.135
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski