Torque teno mini virus 8
Average proteome isoelectric point is 6.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q91C94|Q91C94_9VIRU ORF2 OS=Torque teno mini virus 8 OX=687376 PE=4 SV=1
MM1 pKa = 6.56 STRR4 pKa = 11.84 YY5 pKa = 9.75 IPTKK9 pKa = 10.29 DD10 pKa = 2.9 SHH12 pKa = 5.69 RR13 pKa = 11.84 QKK15 pKa = 10.86 NLKK18 pKa = 8.19 WLNFVTHH25 pKa = 6.29 AHH27 pKa = 7.53 DD28 pKa = 4.22 IFCDD32 pKa = 3.76 CDD34 pKa = 3.82 SPLQHH39 pKa = 5.8 TVANILIQEE48 pKa = 4.2 PNIKK52 pKa = 10.44 FNTQEE57 pKa = 3.75 KK58 pKa = 10.72 DD59 pKa = 3.46 LLKK62 pKa = 10.67 KK63 pKa = 10.76 CLGEE67 pKa = 4.12 TTTTEE72 pKa = 4.1 GAGDD76 pKa = 3.76 PEE78 pKa = 4.62 GFGDD82 pKa = 4.61 GDD84 pKa = 4.02 LEE86 pKa = 4.58 RR87 pKa = 11.84 LFTEE91 pKa = 5.07 DD92 pKa = 4.61 FGEE95 pKa = 4.33 EE96 pKa = 3.96 DD97 pKa = 3.19 TGG99 pKa = 4.08
Molecular weight: 11.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.682
IPC2_protein 4.685
IPC_protein 4.571
Toseland 4.444
ProMoST 4.711
Dawson 4.546
Bjellqvist 4.698
Wikipedia 4.431
Rodwell 4.431
Grimsley 4.355
Solomon 4.546
Lehninger 4.495
Nozaki 4.66
DTASelect 4.838
Thurlkill 4.457
EMBOSS 4.457
Sillero 4.711
Patrickios 3.935
IPC_peptide 4.546
IPC2_peptide 4.698
IPC2.peptide.svr19 4.652
Protein with the highest isoelectric point:
>tr|Q91C94|Q91C94_9VIRU ORF2 OS=Torque teno mini virus 8 OX=687376 PE=4 SV=1
MM1 pKa = 7.79 PWRR4 pKa = 11.84 NYY6 pKa = 8.37 YY7 pKa = 8.77 YY8 pKa = 10.24 RR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 PRR15 pKa = 11.84 RR16 pKa = 11.84 FWRR19 pKa = 11.84 RR20 pKa = 11.84 RR21 pKa = 11.84 FRR23 pKa = 11.84 TPIYY27 pKa = 9.42 RR28 pKa = 11.84 RR29 pKa = 11.84 FWRR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 YY35 pKa = 9.38 RR36 pKa = 11.84 VRR38 pKa = 11.84 KK39 pKa = 8.82 RR40 pKa = 11.84 HH41 pKa = 5.88 FKK43 pKa = 10.65 RR44 pKa = 11.84 KK45 pKa = 9.28 LSKK48 pKa = 10.04 LTIQEE53 pKa = 4.02 WQPPSIRR60 pKa = 11.84 KK61 pKa = 8.27 LTIKK65 pKa = 10.42 GYY67 pKa = 10.22 YY68 pKa = 9.53 PLFQSTRR75 pKa = 11.84 DD76 pKa = 3.43 RR77 pKa = 11.84 LSNNMITYY85 pKa = 10.1 LEE87 pKa = 4.88 SIAPHH92 pKa = 5.51 YY93 pKa = 10.59 VPGGGGFSISCFSLNTLYY111 pKa = 10.67 KK112 pKa = 10.53 EE113 pKa = 4.07 HH114 pKa = 7.13 LKK116 pKa = 10.5 LHH118 pKa = 5.54 NWWTKK123 pKa = 10.15 TNDD126 pKa = 3.19 NYY128 pKa = 11.09 PLIRR132 pKa = 11.84 YY133 pKa = 7.46 NGCTLKK139 pKa = 10.73 LFKK142 pKa = 10.43 SEE144 pKa = 3.83 KK145 pKa = 9.1 VDD147 pKa = 3.57 YY148 pKa = 10.31 IFYY151 pKa = 9.01 YY152 pKa = 9.07 QRR154 pKa = 11.84 HH155 pKa = 5.38 YY156 pKa = 10.69 PMKK159 pKa = 10.46 ASALMYY165 pKa = 10.04 TSTQPQILLLNNRR178 pKa = 11.84 KK179 pKa = 9.94 RR180 pKa = 11.84 IVKK183 pKa = 10.15 CKK185 pKa = 10.64 ANNKK189 pKa = 6.93 KK190 pKa = 7.91 TKK192 pKa = 9.55 PYY194 pKa = 9.32 IRR196 pKa = 11.84 LRR198 pKa = 11.84 IPPPSQLKK206 pKa = 7.91 TKK208 pKa = 9.47 WYY210 pKa = 8.27 FQRR213 pKa = 11.84 DD214 pKa = 3.53 IANLPLLLTIATACSLDD231 pKa = 3.41 RR232 pKa = 11.84 PYY234 pKa = 11.39 LNSQSISNTVGFKK247 pKa = 9.26 TLNYY251 pKa = 9.94 EE252 pKa = 3.83 AFLNRR257 pKa = 11.84 GFKK260 pKa = 10.59 NIPTTGYY267 pKa = 9.66 FPKK270 pKa = 10.47 PNTRR274 pKa = 11.84 YY275 pKa = 10.08 FITQTHH281 pKa = 5.14 STDD284 pKa = 3.51 PKK286 pKa = 9.94 NVPIGSLIFLGNTNLLTLGTKK307 pKa = 9.45 IQDD310 pKa = 3.57 ACPSSTEE317 pKa = 4.15 NEE319 pKa = 3.92 WKK321 pKa = 10.34 NQFEE325 pKa = 5.16 KK326 pKa = 11.16 YY327 pKa = 10.43 LQTEE331 pKa = 4.55 KK332 pKa = 10.88 NWGNPFTPEE341 pKa = 3.91 YY342 pKa = 10.66 LDD344 pKa = 3.71 EE345 pKa = 5.11 DD346 pKa = 4.56 NYY348 pKa = 11.32 NIWITNKK355 pKa = 9.19 TPQEE359 pKa = 3.82 LKK361 pKa = 10.81 AKK363 pKa = 10.1 YY364 pKa = 9.86 KK365 pKa = 11.02 SNTDD369 pKa = 3.45 KK370 pKa = 11.18 LHH372 pKa = 7.29 SSDD375 pKa = 4.78 TDD377 pKa = 3.76 TFWTLKK383 pKa = 10.1 RR384 pKa = 11.84 VPSVLDD390 pKa = 3.13 CRR392 pKa = 11.84 YY393 pKa = 10.83 NPFRR397 pKa = 11.84 DD398 pKa = 3.58 DD399 pKa = 3.55 NRR401 pKa = 11.84 NEE403 pKa = 3.88 VYY405 pKa = 10.52 LLKK408 pKa = 9.76 ITEE411 pKa = 4.15 QNSYY415 pKa = 10.94 DD416 pKa = 3.53 YY417 pKa = 11.04 HH418 pKa = 6.73 EE419 pKa = 4.76 PPKK422 pKa = 10.6 PEE424 pKa = 5.76 LIAKK428 pKa = 8.13 DD429 pKa = 3.97 LPLWACLWGYY439 pKa = 10.57 IDD441 pKa = 4.06 YY442 pKa = 10.5 QKK444 pKa = 11.26 KK445 pKa = 9.23 AATYY449 pKa = 8.67 TEE451 pKa = 4.3 IDD453 pKa = 3.62 TTCICVILSHH463 pKa = 6.98 SLDD466 pKa = 3.86 PTTHH470 pKa = 5.27 TTFIPLDD477 pKa = 3.59 EE478 pKa = 5.69 DD479 pKa = 3.73 FLKK482 pKa = 10.93 GQSSFRR488 pKa = 11.84 PEE490 pKa = 4.09 HH491 pKa = 4.88 QTTRR495 pKa = 11.84 PDD497 pKa = 3.26 EE498 pKa = 4.87 LNWHH502 pKa = 6.7 PKK504 pKa = 10.12 VSFQIQSLNNIACTGPYY521 pKa = 7.83 TAKK524 pKa = 10.59 LPAGTSIEE532 pKa = 3.67 GHH534 pKa = 4.97 MQYY537 pKa = 10.94 KK538 pKa = 9.31 FHH540 pKa = 7.15 FKK542 pKa = 10.65 LGGSPPPMATLTNPEE557 pKa = 4.24 NQPKK561 pKa = 9.78 YY562 pKa = 9.77 PNPDD566 pKa = 2.85 NFLQTTSLQSPTMPIEE582 pKa = 4.45 HH583 pKa = 6.64 YY584 pKa = 10.44 LYY586 pKa = 10.69 NFDD589 pKa = 3.83 EE590 pKa = 4.55 RR591 pKa = 11.84 RR592 pKa = 11.84 HH593 pKa = 5.61 ILTKK597 pKa = 10.51 KK598 pKa = 9.95 AIKK601 pKa = 10.32 RR602 pKa = 11.84 MQTDD606 pKa = 3.4 KK607 pKa = 10.29 EE608 pKa = 4.72 TQSSLLQITDD618 pKa = 3.49 SASTDD623 pKa = 3.04 VPTPYY628 pKa = 10.36 TEE630 pKa = 4.4 TPTSEE635 pKa = 4.67 SEE637 pKa = 4.05 DD638 pKa = 3.62 SEE640 pKa = 4.93 KK641 pKa = 11.19 EE642 pKa = 4.1 EE643 pKa = 3.81 EE644 pKa = 4.25 TLQQRR649 pKa = 11.84 LLKK652 pKa = 9.11 QRR654 pKa = 11.84 KK655 pKa = 6.38 YY656 pKa = 10.73 QKK658 pKa = 9.99 QLQRR662 pKa = 11.84 GINKK666 pKa = 9.67 LLNRR670 pKa = 11.84 LANIQQ675 pKa = 3.37
Molecular weight: 79.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.855
IPC2_protein 9.209
IPC_protein 9.121
Toseland 9.677
ProMoST 9.487
Dawson 9.97
Bjellqvist 9.692
Wikipedia 10.16
Rodwell 10.248
Grimsley 10.058
Solomon 9.984
Lehninger 9.926
Nozaki 9.736
DTASelect 9.663
Thurlkill 9.794
EMBOSS 10.116
Sillero 9.882
Patrickios 4.914
IPC_peptide 9.97
IPC2_peptide 8.375
IPC2.peptide.svr19 8.096
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
774
99
675
387.0
45.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.101 ± 0.034
1.68 ± 0.645
5.039 ± 2.417
5.297 ± 1.812
4.393 ± 0.796
3.747 ± 2.07
2.584 ± 0.695
5.685 ± 0.303
8.01 ± 0.449
9.69 ± 0.286
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.163 ± 0.073
6.46 ± 0.673
7.106 ± 1.464
4.91 ± 0.415
6.46 ± 1.638
5.814 ± 1.329
9.69 ± 0.679
1.809 ± 0.101
1.938 ± 0.443
5.426 ± 2.109
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here