Cordyceps sp. RAO-2017

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Cordycipitaceae; Cordyceps; unclassified Cordyceps

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11255 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2C5ZU16|A0A2C5ZU16_9HYPO NET domain-containing protein OS=Cordyceps sp. RAO-2017 OX=2004951 GN=CDD83_3006 PE=4 SV=1
MM1 pKa = 7.78SDD3 pKa = 2.64QGTPYY8 pKa = 10.86AHH10 pKa = 6.66TPGGMSSTNEE20 pKa = 3.96AFPSIDD26 pKa = 3.25SAGWPRR32 pKa = 11.84EE33 pKa = 3.98FQAPPQNGDD42 pKa = 2.55VDD44 pKa = 4.01SYY46 pKa = 11.75RR47 pKa = 11.84NVPFCDD53 pKa = 4.1GVPSPPMMRR62 pKa = 11.84GGPFQQPLLPQDD74 pKa = 4.43LFGLPATLDD83 pKa = 3.7WNWAEE88 pKa = 4.22MSGGAYY94 pKa = 9.66PSVEE98 pKa = 3.84NGNFGGDD105 pKa = 4.0GPSSMQSS112 pKa = 2.84

Molecular weight:
11.9 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2C5YY38|A0A2C5YY38_9HYPO Uncharacterized protein OS=Cordyceps sp. RAO-2017 OX=2004951 GN=CDD83_8268 PE=4 SV=1
MM1 pKa = 7.08QLLAARR7 pKa = 11.84LARR10 pKa = 11.84PLASSLSSAAAPSVSRR26 pKa = 11.84SLTILTPVRR35 pKa = 11.84PALSPLLQRR44 pKa = 11.84LSPGGSTSFSPVAAADD60 pKa = 3.67VVSPSTVSSHH70 pKa = 6.87PALAGQQLRR79 pKa = 11.84FGPRR83 pKa = 11.84NTMNGHH89 pKa = 5.32TRR91 pKa = 11.84LIQKK95 pKa = 9.37RR96 pKa = 11.84RR97 pKa = 11.84HH98 pKa = 5.13GFLFRR103 pKa = 11.84KK104 pKa = 9.59RR105 pKa = 11.84SKK107 pKa = 8.46TGRR110 pKa = 11.84RR111 pKa = 11.84ILQRR115 pKa = 11.84RR116 pKa = 11.84RR117 pKa = 11.84NKK119 pKa = 9.66GRR121 pKa = 11.84RR122 pKa = 11.84KK123 pKa = 9.56LASS126 pKa = 3.19

Molecular weight:
13.67 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11255

0

11255

4341397

66

4722

385.7

42.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.399 ± 0.028

1.408 ± 0.011

5.957 ± 0.02

5.725 ± 0.024

3.382 ± 0.015

7.517 ± 0.026

2.432 ± 0.01

3.801 ± 0.016

4.123 ± 0.023

9.001 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.134 ± 0.009

2.869 ± 0.013

6.675 ± 0.028

3.874 ± 0.021

7.541 ± 0.025

7.915 ± 0.027

5.211 ± 0.014

6.211 ± 0.018

1.435 ± 0.009

2.355 ± 0.011

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski