Rhodoplanes roseus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Hyphomicrobiaceae; Rhodoplanes

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6350 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A327KGW3|A0A327KGW3_9RHIZ Carbonic anhydrase OS=Rhodoplanes roseus OX=29409 GN=CH341_29280 PE=3 SV=1
MM1 pKa = 7.59TDD3 pKa = 3.78VIVSADD9 pKa = 3.83DD10 pKa = 3.87GDD12 pKa = 4.09VEE14 pKa = 4.7IDD16 pKa = 3.77GEE18 pKa = 4.21ALPEE22 pKa = 3.95VSEE25 pKa = 4.32IAVPAQGPPGPLPFGAVTAWAAGTDD50 pKa = 3.89YY51 pKa = 11.38AADD54 pKa = 3.74SPASVVTFDD63 pKa = 4.02GEE65 pKa = 5.0TYY67 pKa = 10.95VCTADD72 pKa = 3.22HH73 pKa = 6.1TAVAFAADD81 pKa = 4.45LAAHH85 pKa = 6.87RR86 pKa = 11.84WLLIARR92 pKa = 11.84KK93 pKa = 9.34GNQGPTGTAGPQGVPGPFAFDD114 pKa = 4.19TIVPWTTATAYY125 pKa = 10.08HH126 pKa = 6.27ATPPASAVV134 pKa = 3.26

Molecular weight: 13.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A327K6U4|A0A327K6U4_9RHIZ Uncharacterized protein (Fragment) OS=Rhodoplanes roseus OX=29409 GN=CH341_31395 PE=4 SV=1
PP1 pKa = 7.52LATAPPGRR9 pKa = 11.84AALGRR14 pKa = 11.84AALRR18 pKa = 11.84TAALGRR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84TLAGALLRR34 pKa = 11.84CAASSRR40 pKa = 11.84PRR42 pKa = 11.84AIRR45 pKa = 11.84TGPLGALTLVARR57 pKa = 11.84RR58 pKa = 11.84RR59 pKa = 11.84RR60 pKa = 11.84VAAATLALLLALLAAVLAALGTFLPTIIGLVLVARR95 pKa = 11.84RR96 pKa = 11.84TAPVGIRR103 pKa = 11.84TGALALVVPRR113 pKa = 11.84IVAGVVPIASIRR125 pKa = 11.84ALAGIVAVPRR135 pKa = 11.84TGALASVVAITRR147 pKa = 11.84TGAFALRR154 pKa = 11.84RR155 pKa = 11.84GAVAPLARR163 pKa = 11.84GRR165 pKa = 11.84AALAVAGPGG174 pKa = 3.26

Molecular weight: 17.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6350

0

6350

1912495

25

3691

301.2

32.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.65 ± 0.049

0.855 ± 0.01

5.613 ± 0.029

5.208 ± 0.032

3.487 ± 0.019

8.809 ± 0.034

1.949 ± 0.016

4.676 ± 0.022

2.811 ± 0.024

10.062 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.26 ± 0.014

2.081 ± 0.017

5.744 ± 0.026

2.726 ± 0.023

7.743 ± 0.038

4.983 ± 0.027

5.634 ± 0.04

8.363 ± 0.028

1.317 ± 0.012

2.028 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski