Enterobius vermicularis (Human pinworm)
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12840 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0N4VN62|A0A0N4VN62_ENTVE Uncharacterized protein OS=Enterobius vermicularis OX=51028 GN=EVEC_LOCUS11608 PE=4 SV=1
MM1 pKa = 7.65 GKK3 pKa = 9.07 GQKK6 pKa = 9.57 VEE8 pKa = 4.13 EE9 pKa = 4.28 EE10 pKa = 4.01 EE11 pKa = 4.1 GGIRR15 pKa = 11.84 SGCRR19 pKa = 11.84 DD20 pKa = 3.37 MMTTDD25 pKa = 3.64 LRR27 pKa = 11.84 IHH29 pKa = 7.3 RR30 pKa = 11.84 MTAVTTDD37 pKa = 3.15 IVPNTNNWISCKK49 pKa = 8.55 WFSVGIHH56 pKa = 5.27 WVCVCVSVGCCSACINDD73 pKa = 4.47 IYY75 pKa = 11.0 DD76 pKa = 3.73 CSVPLPTNDD85 pKa = 4.24 DD86 pKa = 3.2 GDD88 pKa = 4.23 YY89 pKa = 11.47 VDD91 pKa = 6.23 DD92 pKa = 6.89 DD93 pKa = 6.21 DD94 pKa = 7.7 GDD96 pKa = 4.27 DD97 pKa = 4.88 DD98 pKa = 5.1 NDD100 pKa = 4.34 GGGGDD105 pKa = 5.8 DD106 pKa = 6.41 DD107 pKa = 7.04 DD108 pKa = 7.8 DD109 pKa = 7.59 DD110 pKa = 7.6 DD111 pKa = 7.52 DD112 pKa = 7.38 DD113 pKa = 7.43 DD114 pKa = 7.45 GDD116 pKa = 4.09 DD117 pKa = 5.03 DD118 pKa = 4.66 YY119 pKa = 12.13 GGDD122 pKa = 3.16 HH123 pKa = 6.41 HH124 pKa = 7.3 HH125 pKa = 7.7 RR126 pKa = 11.84 YY127 pKa = 9.33 QSS129 pKa = 3.01
Molecular weight: 14.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.711
IPC2_protein 3.668
IPC_protein 3.719
Toseland 3.478
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.732
Rodwell 3.541
Grimsley 3.376
Solomon 3.732
Lehninger 3.694
Nozaki 3.859
DTASelect 4.19
Thurlkill 3.554
EMBOSS 3.732
Sillero 3.846
Patrickios 0.668
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.754
Protein with the highest isoelectric point:
>tr|A0A158Q9W8|A0A158Q9W8_ENTVE Diadenosine tetraphosphate synthetase OS=Enterobius vermicularis OX=51028 GN=EVEC_LOCUS3065 PE=3 SV=1
MM1 pKa = 7.48 RR2 pKa = 11.84 RR3 pKa = 11.84 RR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 RR7 pKa = 11.84 GRR9 pKa = 11.84 RR10 pKa = 11.84 GRR12 pKa = 11.84 RR13 pKa = 11.84 GRR15 pKa = 11.84 RR16 pKa = 11.84 RR17 pKa = 11.84 RR18 pKa = 11.84 RR19 pKa = 11.84 SRR21 pKa = 11.84 RR22 pKa = 11.84 KK23 pKa = 8.73 RR24 pKa = 11.84 RR25 pKa = 11.84 QKK27 pKa = 10.11 RR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 SINTVIISPPKK42 pKa = 10.06 SSALSFGNRR51 pKa = 11.84 HH52 pKa = 5.34 SQQQQRR58 pKa = 11.84 LITRR62 pKa = 11.84 KK63 pKa = 9.31 PYY65 pKa = 10.84 GIVVV69 pKa = 3.84
Molecular weight: 8.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.447
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.647
Grimsley 13.115
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.354
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.266
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12840
0
12840
4826947
29
7647
375.9
42.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.537 ± 0.017
2.3 ± 0.021
5.287 ± 0.017
6.815 ± 0.03
4.592 ± 0.017
5.525 ± 0.024
2.145 ± 0.009
5.59 ± 0.014
6.307 ± 0.024
9.401 ± 0.029
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.237 ± 0.01
4.605 ± 0.014
4.488 ± 0.026
3.864 ± 0.018
5.615 ± 0.02
8.124 ± 0.025
5.422 ± 0.022
6.644 ± 0.018
1.114 ± 0.008
3.293 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here