Vibrio virus vB_VspP_SBP1
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 112 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3T0IIP4|A0A3T0IIP4_9CAUD HNH endonuclease OS=Vibrio virus vB_VspP_SBP1 OX=2500581 GN=SBP1_gp042 PE=4 SV=1
MM1 pKa = 7.33 TLLDD5 pKa = 3.81 VFNYY9 pKa = 10.18 LALGPLANTGDD20 pKa = 4.08 VVNGMIKK27 pKa = 9.29 TDD29 pKa = 3.64 SYY31 pKa = 11.76 QKK33 pKa = 10.4 FANFTQLGLRR43 pKa = 11.84 DD44 pKa = 3.43 IYY46 pKa = 11.29 TRR48 pKa = 11.84 FPLKK52 pKa = 10.59 DD53 pKa = 3.22 SEE55 pKa = 5.65 LILDD59 pKa = 3.77 QDD61 pKa = 4.46 DD62 pKa = 4.08 GTVIYY67 pKa = 10.16 YY68 pKa = 10.6 LNSKK72 pKa = 8.92 HH73 pKa = 6.62 AYY75 pKa = 9.54 SNTEE79 pKa = 3.69 STEE82 pKa = 3.68 QKK84 pKa = 11.0 YY85 pKa = 10.49 IDD87 pKa = 5.13 DD88 pKa = 4.83 DD89 pKa = 4.62 DD90 pKa = 6.08 GNEE93 pKa = 3.95 FTDD96 pKa = 5.67 DD97 pKa = 3.45 ILAITAFYY105 pKa = 11.06 DD106 pKa = 3.51 EE107 pKa = 5.4 AGCLVPINDD116 pKa = 3.57 NSASNSYY123 pKa = 7.69 YY124 pKa = 10.17 TPTPLSVQIPTPVTGNSTFVQYY146 pKa = 10.73 RR147 pKa = 11.84 ADD149 pKa = 3.72 HH150 pKa = 6.74 PKK152 pKa = 10.72 LSDD155 pKa = 3.35 VSDD158 pKa = 4.02 PSSVEE163 pKa = 3.81 LEE165 pKa = 4.18 LPEE168 pKa = 4.31 VALSALLCYY177 pKa = 7.86 VTWRR181 pKa = 11.84 AYY183 pKa = 9.56 MPNQSAEE190 pKa = 3.96 AQAISMNQKK199 pKa = 9.06 MMYY202 pKa = 9.95 EE203 pKa = 4.01 EE204 pKa = 4.18 QLLFLQKK211 pKa = 10.81 YY212 pKa = 7.11 ITVNNISPEE221 pKa = 4.05 ANNAFHH227 pKa = 7.3 SNGWVV232 pKa = 2.92
Molecular weight: 26.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.892
IPC2_protein 4.062
IPC_protein 4.05
Toseland 3.834
ProMoST 4.202
Dawson 4.024
Bjellqvist 4.177
Wikipedia 3.961
Rodwell 3.872
Grimsley 3.745
Solomon 4.024
Lehninger 3.986
Nozaki 4.139
DTASelect 4.368
Thurlkill 3.884
EMBOSS 3.961
Sillero 4.164
Patrickios 0.604
IPC_peptide 4.024
IPC2_peptide 4.139
IPC2.peptide.svr19 4.046
Protein with the highest isoelectric point:
>tr|A0A3T0IIF9|A0A3T0IIF9_9CAUD DNA binding protein OS=Vibrio virus vB_VspP_SBP1 OX=2500581 GN=SBP1_gp025 PE=4 SV=1
MM1 pKa = 7.69 LAATYY6 pKa = 11.01 ANRR9 pKa = 11.84 TVEE12 pKa = 3.82 AQEE15 pKa = 3.82 QVRR18 pKa = 11.84 CVITLSVHH26 pKa = 5.56 SASKK30 pKa = 10.66 SGINTVRR37 pKa = 11.84 VLMRR41 pKa = 11.84 TKK43 pKa = 9.81 LTRR46 pKa = 11.84 KK47 pKa = 9.61 IIVKK51 pKa = 10.27 SAVLYY56 pKa = 11.0 QNTLVNLMLTIRR68 pKa = 11.84 MVITT72 pKa = 3.83
Molecular weight: 8.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.292
IPC2_protein 10.072
IPC_protein 11.052
Toseland 11.14
ProMoST 11.155
Dawson 11.199
Bjellqvist 11.008
Wikipedia 11.491
Rodwell 11.345
Grimsley 11.242
Solomon 11.462
Lehninger 11.403
Nozaki 11.125
DTASelect 11.008
Thurlkill 11.14
EMBOSS 11.579
Sillero 11.155
Patrickios 11.125
IPC_peptide 11.462
IPC2_peptide 10.248
IPC2.peptide.svr19 8.361
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
112
0
112
24106
47
3548
215.2
24.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.326 ± 0.393
1.207 ± 0.234
6.48 ± 0.216
6.799 ± 0.301
3.576 ± 0.128
6.355 ± 0.231
2.161 ± 0.221
5.629 ± 0.206
6.438 ± 0.318
8.089 ± 0.233
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.02 ± 0.163
5.476 ± 0.185
3.622 ± 0.197
4.372 ± 0.228
4.663 ± 0.214
5.418 ± 0.2
6.604 ± 0.297
6.534 ± 0.187
1.286 ± 0.127
3.945 ± 0.186
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here