Octadecabacter sp. SW4
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3350 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5B9ENM2|A0A5B9ENM2_9RHOB Molybdenum import ATP-binding protein ModC OS=Octadecabacter sp. SW4 OX=2602067 GN=modC PE=3 SV=1
MM1 pKa = 6.7 QAAAGFRR8 pKa = 11.84 RR9 pKa = 11.84 VWPSLLIKK17 pKa = 10.29 GIGMTAITTNVQGYY31 pKa = 5.82 GTQWFSAPGDD41 pKa = 3.83 NLHH44 pKa = 6.24 VLSTGLLGSWDD55 pKa = 4.03 TLTALASHH63 pKa = 7.45 DD64 pKa = 3.84 NLRR67 pKa = 11.84 VDD69 pKa = 4.55 NSGQMWSMASVLEE82 pKa = 4.1 VRR84 pKa = 11.84 GSDD87 pKa = 3.31 VRR89 pKa = 11.84 TANYY93 pKa = 10.01 EE94 pKa = 3.92 GGLMQSEE101 pKa = 4.57 SDD103 pKa = 3.57 SHH105 pKa = 7.83 LDD107 pKa = 3.21 AVIRR111 pKa = 11.84 YY112 pKa = 9.02 KK113 pKa = 11.21 GEE115 pKa = 3.83 AVGGQIFNFGTINAYY130 pKa = 8.39 WAVGVEE136 pKa = 3.97 IGTTGTTFDD145 pKa = 3.59 NRR147 pKa = 11.84 GLIDD151 pKa = 4.1 AGSALRR157 pKa = 11.84 MGLDD161 pKa = 3.48 GADD164 pKa = 3.45 GQVVRR169 pKa = 11.84 NFGTMISAGNASAAGDD185 pKa = 3.53 ATYY188 pKa = 11.33 GNGVYY193 pKa = 10.18 VEE195 pKa = 4.46 GSNNLFYY202 pKa = 11.11 NWTGSTLTVTSGGAGVAFGGAGTGNSVKK230 pKa = 10.57 NAGTITSDD238 pKa = 3.41 TGWGVDD244 pKa = 3.74 LSGLAVGVSADD255 pKa = 3.74 VINTRR260 pKa = 11.84 GGVIAGGAGAVRR272 pKa = 11.84 GNSAEE277 pKa = 3.89 NVIYY281 pKa = 10.75 NSGIMNGDD289 pKa = 2.77 VWLYY293 pKa = 11.28 GGDD296 pKa = 4.19 DD297 pKa = 3.91 YY298 pKa = 12.11 VSSGTYY304 pKa = 10.02 AQFNGDD310 pKa = 3.98 LYY312 pKa = 11.27 AGNGNDD318 pKa = 3.41 TLYY321 pKa = 11.33 GSGGDD326 pKa = 3.61 DD327 pKa = 2.92 SFYY330 pKa = 11.3 GQGQNDD336 pKa = 4.73 EE337 pKa = 3.88 IWGFDD342 pKa = 3.2 GDD344 pKa = 4.51 DD345 pKa = 3.83 FLFGGAGDD353 pKa = 4.29 DD354 pKa = 4.17 VIEE357 pKa = 5.03 GGDD360 pKa = 3.51 GADD363 pKa = 3.56 YY364 pKa = 11.23 LRR366 pKa = 11.84 GQGGSDD372 pKa = 3.28 TLNGGEE378 pKa = 4.78 GDD380 pKa = 3.72 DD381 pKa = 5.17 DD382 pKa = 4.31 IYY384 pKa = 11.52 GGVGADD390 pKa = 2.57 IFVFEE395 pKa = 4.41 QNGNFDD401 pKa = 5.54 IIWDD405 pKa = 3.91 FQNNVDD411 pKa = 5.46 QIDD414 pKa = 4.06 LSDD417 pKa = 4.52 LGITSYY423 pKa = 11.29 SALAAVMTTVGADD436 pKa = 3.39 VQIDD440 pKa = 4.18 LSSLSGVYY448 pKa = 10.64 GDD450 pKa = 4.2 IIEE453 pKa = 4.57 LRR455 pKa = 11.84 GSVTIGDD462 pKa = 4.51 LDD464 pKa = 4.63 AGDD467 pKa = 5.24 FILL470 pKa = 5.92
Molecular weight: 48.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.683
IPC2_protein 3.592
IPC_protein 3.656
Toseland 3.401
ProMoST 3.834
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.643
Rodwell 3.478
Grimsley 3.312
Solomon 3.656
Lehninger 3.617
Nozaki 3.77
DTASelect 4.088
Thurlkill 3.478
EMBOSS 3.643
Sillero 3.783
Patrickios 1.265
IPC_peptide 3.656
IPC2_peptide 3.745
IPC2.peptide.svr19 3.7
Protein with the highest isoelectric point:
>tr|A0A5B9ENQ5|A0A5B9ENQ5_9RHOB Cell division protein FtsQ OS=Octadecabacter sp. SW4 OX=2602067 GN=ftsQ PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNRR10 pKa = 11.84 VRR12 pKa = 11.84 KK13 pKa = 8.99 NRR15 pKa = 11.84 HH16 pKa = 3.77 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.56 GRR39 pKa = 11.84 KK40 pKa = 8.73 VLSAA44 pKa = 4.05
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3350
0
3350
1034289
32
2756
308.7
33.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.518 ± 0.058
0.882 ± 0.013
6.598 ± 0.044
5.205 ± 0.042
3.824 ± 0.03
8.757 ± 0.048
2.03 ± 0.023
5.481 ± 0.029
3.072 ± 0.038
9.767 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.907 ± 0.02
2.693 ± 0.026
4.882 ± 0.028
3.294 ± 0.02
6.42 ± 0.038
4.854 ± 0.025
5.755 ± 0.029
7.386 ± 0.037
1.414 ± 0.02
2.261 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here