Puma concolor (Mountain lion)
Average proteome isoelectric point is 6.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 22836 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6P6HL58|A0A6P6HL58_PUMCO Isoform of A0A6P6HKZ6 contactin-associated protein-like 4 isoform X2 OS=Puma concolor OX=9696 GN=CNTNAP4 PE=3 SV=1
MM1 pKa = 7.22 EE2 pKa = 5.09 AVVLIPYY9 pKa = 9.99 VLGLLLLPLLAVVLCVRR26 pKa = 11.84 CRR28 pKa = 11.84 EE29 pKa = 4.27 LPGSYY34 pKa = 10.75 DD35 pKa = 3.3 NTASDD40 pKa = 3.94 SLAPSSIVIKK50 pKa = 10.5 RR51 pKa = 11.84 PPTLATWTPATSYY64 pKa = 10.76 PPVTSYY70 pKa = 11.28 PPLSQPDD77 pKa = 4.23 LLPIPRR83 pKa = 11.84 SPQPPGGSHH92 pKa = 6.82 RR93 pKa = 11.84 MPSSRR98 pKa = 11.84 QDD100 pKa = 2.92 SDD102 pKa = 3.47 GANSVASYY110 pKa = 10.11 EE111 pKa = 4.13 NEE113 pKa = 4.24 GASGAPGALVAGRR126 pKa = 11.84 LGPGLGPADD135 pKa = 3.58 RR136 pKa = 11.84 CVVTSEE142 pKa = 4.45 PACEE146 pKa = 5.39 DD147 pKa = 3.57 DD148 pKa = 6.66 DD149 pKa = 4.6 EE150 pKa = 6.88 DD151 pKa = 5.49 EE152 pKa = 4.94 EE153 pKa = 4.44 EE154 pKa = 4.91 DD155 pKa = 4.15 YY156 pKa = 11.46 PNEE159 pKa = 4.39 GYY161 pKa = 11.04 LEE163 pKa = 4.18 VLPDD167 pKa = 3.64 STPTTGTAVPPAPAPSNPGLRR188 pKa = 11.84 DD189 pKa = 3.14 SAFSMEE195 pKa = 4.23 SGEE198 pKa = 4.99 DD199 pKa = 3.63 YY200 pKa = 11.66 VNVPEE205 pKa = 4.61 SEE207 pKa = 4.19 EE208 pKa = 4.19 SADD211 pKa = 3.6 VSLDD215 pKa = 3.29 GSRR218 pKa = 11.84 EE219 pKa = 3.96 YY220 pKa = 11.72 VNVSQEE226 pKa = 4.24 LPPVARR232 pKa = 11.84 TEE234 pKa = 3.93 PAILSSQNDD243 pKa = 3.92 DD244 pKa = 4.02 EE245 pKa = 5.46 EE246 pKa = 5.21 EE247 pKa = 4.2 GAPDD251 pKa = 3.75 YY252 pKa = 11.56 EE253 pKa = 4.31 NLQGLNN259 pKa = 3.55
Molecular weight: 27.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.716
IPC2_protein 3.808
IPC_protein 3.783
Toseland 3.579
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.656
Rodwell 3.617
Grimsley 3.49
Solomon 3.745
Lehninger 3.694
Nozaki 3.859
DTASelect 4.037
Thurlkill 3.617
EMBOSS 3.668
Sillero 3.897
Patrickios 1.125
IPC_peptide 3.745
IPC2_peptide 3.884
IPC2.peptide.svr19 3.804
Protein with the highest isoelectric point:
>tr|A0A6P6HM51|A0A6P6HM51_PUMCO Thioredoxin-like protein OS=Puma concolor OX=9696 GN=TXNL4B PE=3 SV=1
MM1 pKa = 7.51 GPGSGPNLRR10 pKa = 11.84 AGVLLPSGNGPPNPRR25 pKa = 11.84 PVGLGPGPSPNLRR38 pKa = 11.84 SGFVGTNPAPRR49 pKa = 11.84 SGMFPGPGLGPNPRR63 pKa = 11.84 ANSLGPGLGPNPRR76 pKa = 11.84 AGGLGPGPNLDD87 pKa = 3.45 TRR89 pKa = 11.84 AGGLLGTGSGLNLRR103 pKa = 11.84 MAGPQGLDD111 pKa = 3.09 LAPILRR117 pKa = 11.84 AAGLLGANSAAFSQASGNMGTSPSSMARR145 pKa = 11.84 VPGPMGPNSGPGSRR159 pKa = 11.84 GIGLPGPNPSPMSRR173 pKa = 11.84 APGPIGPNSAHH184 pKa = 7.19 FSRR187 pKa = 11.84 PAGPMGVNASPFPRR201 pKa = 11.84 GTGSGGLNPAAFSQSSGTLASNPATFQRR229 pKa = 11.84 SAGLQGSSPAIFPRR243 pKa = 11.84 TSGPLGPNPANFPRR257 pKa = 11.84 ASGLQGPSPAAFPRR271 pKa = 11.84 STGPLGPGQVTFPRR285 pKa = 11.84 SAPGPLGSSPAGPVGINPAPFARR308 pKa = 11.84 PTGTLGLNPASFPRR322 pKa = 11.84 MNGPVSKK329 pKa = 9.32 TLVPFPRR336 pKa = 11.84 VGNLPGTNPAAFPRR350 pKa = 11.84 PGGPMAAMYY359 pKa = 9.22 PNGMLPPP366 pKa = 4.73
Molecular weight: 35.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.423
IPC2_protein 11.008
IPC_protein 12.515
Toseland 12.676
ProMoST 13.173
Dawson 12.676
Bjellqvist 12.676
Wikipedia 13.159
Rodwell 12.193
Grimsley 12.72
Solomon 13.173
Lehninger 13.071
Nozaki 12.676
DTASelect 12.676
Thurlkill 12.676
EMBOSS 13.173
Sillero 12.676
Patrickios 11.915
IPC_peptide 13.173
IPC2_peptide 12.164
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
17614
5222
22836
13162948
31
34365
576.4
64.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.857 ± 0.016
2.194 ± 0.015
4.837 ± 0.011
7.114 ± 0.023
3.691 ± 0.013
6.517 ± 0.023
2.57 ± 0.01
4.481 ± 0.016
5.812 ± 0.025
9.887 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.174 ± 0.008
3.659 ± 0.012
6.174 ± 0.022
4.699 ± 0.016
5.664 ± 0.017
8.312 ± 0.025
5.35 ± 0.018
6.117 ± 0.018
1.194 ± 0.006
2.675 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here