Amino acid dipepetide frequency for Puma concolor (Mountain lion)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.563AlaAla: 6.563 ± 0.042	1.402AlaCys: 1.402 ± 0.014	2.913AlaAsp: 2.913 ± 0.018	4.677AlaGlu: 4.677 ± 0.026	2.699AlaPhe: 2.699 ± 0.018	4.723AlaGly: 4.723 ± 0.026	1.509AlaHis: 1.509 ± 0.015	2.825AlaIle: 2.825 ± 0.017	3.39AlaLys: 3.39 ± 0.024	7.078AlaLeu: 7.078 ± 0.045	1.48AlaMet: 1.48 ± 0.012	2.018AlaAsn: 2.018 ± 0.015	4.094AlaPro: 4.094 ± 0.031	3.167AlaGln: 3.167 ± 0.023	3.657AlaArg: 3.657 ± 0.024	5.726AlaSer: 5.726 ± 0.032	3.57AlaThr: 3.57 ± 0.022	4.735AlaVal: 4.735 ± 0.026	0.791AlaTrp: 0.791 ± 0.009	1.541AlaTyr: 1.541 ± 0.014	0.012AlaXaa: 0.012 ± 0.001
Cys	1.252CysAla: 1.252 ± 0.013	0.626CysCys: 0.626 ± 0.013	1.017CysAsp: 1.017 ± 0.011	1.267CysGlu: 1.267 ± 0.015	0.851CysPhe: 0.851 ± 0.01	1.828CysGly: 1.828 ± 0.026	0.658CysHis: 0.658 ± 0.008	0.96CysIle: 0.96 ± 0.01	1.147CysLys: 1.147 ± 0.017	2.148CysLeu: 2.148 ± 0.022	0.415CysMet: 0.415 ± 0.007	0.818CysAsn: 0.818 ± 0.012	1.376CysPro: 1.376 ± 0.019	1.042CysGln: 1.042 ± 0.015	1.289CysArg: 1.289 ± 0.012	1.987CysSer: 1.987 ± 0.017	1.075CysThr: 1.075 ± 0.013	1.313CysVal: 1.313 ± 0.013	0.287CysTrp: 0.287 ± 0.005	0.581CysTyr: 0.581 ± 0.007	0.005CysXaa: 0.005 ± 0.001
Asp	2.804AspAla: 2.804 ± 0.016	1.055AspCys: 1.055 ± 0.011	2.618AspAsp: 2.618 ± 0.018	3.422AspGlu: 3.422 ± 0.021	2.137AspPhe: 2.137 ± 0.016	3.31AspGly: 3.31 ± 0.029	1.141AspHis: 1.141 ± 0.011	2.597AspIle: 2.597 ± 0.02	2.492AspLys: 2.492 ± 0.015	4.977AspLeu: 4.977 ± 0.023	1.103AspMet: 1.103 ± 0.009	1.67AspAsn: 1.67 ± 0.015	2.951AspPro: 2.951 ± 0.017	1.819AspGln: 1.819 ± 0.012	2.486AspArg: 2.486 ± 0.018	4.155AspSer: 4.155 ± 0.025	2.468AspThr: 2.468 ± 0.017	3.083AspVal: 3.083 ± 0.024	0.616AspTrp: 0.616 ± 0.008	1.457AspTyr: 1.457 ± 0.013	0.006AspXaa: 0.006 ± 0.001
Glu	5.17GluAla: 5.17 ± 0.028	1.494GluCys: 1.494 ± 0.031	4.387GluAsp: 4.387 ± 0.024	7.912GluGlu: 7.912 ± 0.055	2.068GluPhe: 2.068 ± 0.016	4.234GluGly: 4.234 ± 0.026	1.507GluHis: 1.507 ± 0.013	3.193GluIle: 3.193 ± 0.026	5.386GluLys: 5.386 ± 0.04	6.308GluLeu: 6.308 ± 0.035	1.648GluMet: 1.648 ± 0.013	3.167GluAsn: 3.167 ± 0.026	3.255GluPro: 3.255 ± 0.031	3.096GluGln: 3.096 ± 0.024	3.987GluArg: 3.987 ± 0.027	4.456GluSer: 4.456 ± 0.026	3.402GluThr: 3.402 ± 0.021	4.165GluVal: 4.165 ± 0.028	0.706GluTrp: 0.706 ± 0.008	1.594GluTyr: 1.594 ± 0.024	0.008GluXaa: 0.008 ± 0.001
Phe	2.007PheAla: 2.007 ± 0.013	0.98PheCys: 0.98 ± 0.01	1.667PheAsp: 1.667 ± 0.012	2.02PheGlu: 2.02 ± 0.015	1.654PhePhe: 1.654 ± 0.016	2.231PheGly: 2.231 ± 0.018	1.046PheHis: 1.046 ± 0.01	1.907PheIle: 1.907 ± 0.015	1.784PheLys: 1.784 ± 0.016	4.207PheLeu: 4.207 ± 0.027	0.796PheMet: 0.796 ± 0.007	1.34PheAsn: 1.34 ± 0.013	2.023PhePro: 2.023 ± 0.017	1.764PheGln: 1.764 ± 0.014	2.0PheArg: 2.0 ± 0.023	3.49PheSer: 3.49 ± 0.021	2.05PheThr: 2.05 ± 0.017	2.228PheVal: 2.228 ± 0.017	0.498PheTrp: 0.498 ± 0.007	1.206PheTyr: 1.206 ± 0.011	0.008PheXaa: 0.008 ± 0.001
Gly	4.486GlyAla: 4.486 ± 0.031	1.3GlyCys: 1.3 ± 0.015	3.137GlyAsp: 3.137 ± 0.02	4.228GlyGlu: 4.228 ± 0.031	2.435GlyPhe: 2.435 ± 0.019	5.073GlyGly: 5.073 ± 0.044	1.702GlyHis: 1.702 ± 0.015	2.813GlyIle: 2.813 ± 0.018	3.882GlyLys: 3.882 ± 0.031	5.923GlyLeu: 5.923 ± 0.032	1.295GlyMet: 1.295 ± 0.012	2.413GlyAsn: 2.413 ± 0.016	4.329GlyPro: 4.329 ± 0.05	2.779GlyGln: 2.779 ± 0.017	3.876GlyArg: 3.876 ± 0.021	5.782GlySer: 5.782 ± 0.031	3.621GlyThr: 3.621 ± 0.026	3.572GlyVal: 3.572 ± 0.018	0.782GlyTrp: 0.782 ± 0.009	1.73GlyTyr: 1.73 ± 0.014	0.014GlyXaa: 0.014 ± 0.001
His	1.32HisAla: 1.32 ± 0.013	0.692HisCys: 0.692 ± 0.009	0.878HisAsp: 0.878 ± 0.009	1.294HisGlu: 1.294 ± 0.011	1.093HisPhe: 1.093 ± 0.009	1.547HisGly: 1.547 ± 0.014	0.874HisHis: 0.874 ± 0.013	1.254HisIle: 1.254 ± 0.011	1.237HisLys: 1.237 ± 0.012	2.93HisLeu: 2.93 ± 0.02	0.585HisMet: 0.585 ± 0.008	0.84HisAsn: 0.84 ± 0.009	1.623HisPro: 1.623 ± 0.014	1.375HisGln: 1.375 ± 0.02	1.634HisArg: 1.634 ± 0.016	2.264HisSer: 2.264 ± 0.017	1.587HisThr: 1.587 ± 0.02	1.516HisVal: 1.516 ± 0.012	0.343HisTrp: 0.343 ± 0.005	0.814HisTyr: 0.814 ± 0.009	0.004HisXaa: 0.004 ± 0.001
Ile	2.606IleAla: 2.606 ± 0.017	1.111IleCys: 1.111 ± 0.01	1.991IleAsp: 1.991 ± 0.016	2.614IleGlu: 2.614 ± 0.025	1.957IlePhe: 1.957 ± 0.017	2.23IleGly: 2.23 ± 0.016	1.412IleHis: 1.412 ± 0.019	2.455IleIle: 2.455 ± 0.021	2.626IleLys: 2.626 ± 0.026	4.711IleLeu: 4.711 ± 0.023	1.026IleMet: 1.026 ± 0.01	1.83IleAsn: 1.83 ± 0.013	2.65IlePro: 2.65 ± 0.018	2.311IleGln: 2.311 ± 0.016	2.397IleArg: 2.397 ± 0.017	3.763IleSer: 3.763 ± 0.022	2.602IleThr: 2.602 ± 0.029	2.565IleVal: 2.565 ± 0.024	0.518IleTrp: 0.518 ± 0.007	1.428IleTyr: 1.428 ± 0.011	0.006IleXaa: 0.006 ± 0.001
Lys	4.021LysAla: 4.021 ± 0.026	1.134LysCys: 1.134 ± 0.014	3.17LysAsp: 3.17 ± 0.025	5.132LysGlu: 5.132 ± 0.035	1.704LysPhe: 1.704 ± 0.014	3.256LysGly: 3.256 ± 0.03	1.388LysHis: 1.388 ± 0.013	2.811LysIle: 2.811 ± 0.023	4.697LysLys: 4.697 ± 0.037	5.152LysLeu: 5.152 ± 0.026	1.475LysMet: 1.475 ± 0.012	2.414LysAsn: 2.414 ± 0.018	3.192LysPro: 3.192 ± 0.034	2.592LysGln: 2.592 ± 0.02	3.247LysArg: 3.247 ± 0.023	3.954LysSer: 3.954 ± 0.023	3.131LysThr: 3.131 ± 0.022	3.482LysVal: 3.482 ± 0.035	0.608LysTrp: 0.608 ± 0.009	1.551LysTyr: 1.551 ± 0.015	0.007LysXaa: 0.007 ± 0.001
Leu	6.787LeuAla: 6.787 ± 0.044	2.175LeuCys: 2.175 ± 0.02	4.71LeuAsp: 4.71 ± 0.023	7.12LeuGlu: 7.12 ± 0.035	3.476LeuPhe: 3.476 ± 0.022	5.94LeuGly: 5.94 ± 0.037	2.722LeuHis: 2.722 ± 0.017	4.002LeuIle: 4.002 ± 0.025	5.805LeuLys: 5.805 ± 0.028	10.674LeuLeu: 10.674 ± 0.064	2.023LeuMet: 2.023 ± 0.015	3.521LeuAsn: 3.521 ± 0.022	5.954LeuPro: 5.954 ± 0.038	5.609LeuGln: 5.609 ± 0.037	5.962LeuArg: 5.962 ± 0.032	7.995LeuSer: 7.995 ± 0.034	5.125LeuThr: 5.125 ± 0.022	5.579LeuVal: 5.579 ± 0.031	1.142LeuTrp: 1.142 ± 0.012	2.536LeuTyr: 2.536 ± 0.019	0.014LeuXaa: 0.014 ± 0.001
Met	1.978MetAla: 1.978 ± 0.015	0.404MetCys: 0.404 ± 0.006	1.251MetAsp: 1.251 ± 0.011	1.806MetGlu: 1.806 ± 0.015	0.752MetPhe: 0.752 ± 0.009	1.323MetGly: 1.323 ± 0.011	0.462MetHis: 0.462 ± 0.006	0.851MetIle: 0.851 ± 0.01	1.429MetLys: 1.429 ± 0.011	1.977MetLeu: 1.977 ± 0.013	0.581MetMet: 0.581 ± 0.008	0.905MetAsn: 0.905 ± 0.01	1.083MetPro: 1.083 ± 0.012	0.898MetGln: 0.898 ± 0.01	1.035MetArg: 1.035 ± 0.01	1.562MetSer: 1.562 ± 0.011	1.151MetThr: 1.151 ± 0.01	1.408MetVal: 1.408 ± 0.012	0.237MetTrp: 0.237 ± 0.004	0.643MetTyr: 0.643 ± 0.008	0.003MetXaa: 0.003 ± 0.001
Asn	2.046AsnAla: 2.046 ± 0.014	0.827AsnCys: 0.827 ± 0.01	1.534AsnAsp: 1.534 ± 0.014	2.214AsnGlu: 2.214 ± 0.019	1.478AsnPhe: 1.478 ± 0.012	2.414AsnGly: 2.414 ± 0.02	0.949AsnHis: 0.949 ± 0.009	2.14AsnIle: 2.14 ± 0.018	2.227AsnLys: 2.227 ± 0.019	3.746AsnLeu: 3.746 ± 0.022	0.908AsnMet: 0.908 ± 0.01	1.522AsnAsn: 1.522 ± 0.015	2.232AsnPro: 2.232 ± 0.016	1.633AsnGln: 1.633 ± 0.014	1.842AsnArg: 1.842 ± 0.011	3.128AsnSer: 3.128 ± 0.02	1.948AsnThr: 1.948 ± 0.012	2.239AsnVal: 2.239 ± 0.017	0.449AsnTrp: 0.449 ± 0.006	1.107AsnTyr: 1.107 ± 0.012	0.005AsnXaa: 0.005 ± 0.001
Pro	4.757ProAla: 4.757 ± 0.034	1.132ProCys: 1.132 ± 0.016	2.774ProAsp: 2.774 ± 0.022	4.478ProGlu: 4.478 ± 0.029	1.965ProPhe: 1.965 ± 0.015	5.327ProGly: 5.327 ± 0.065	1.44ProHis: 1.44 ± 0.012	1.896ProIle: 1.896 ± 0.021	2.833ProLys: 2.833 ± 0.027	5.278ProLeu: 5.278 ± 0.029	1.092ProMet: 1.092 ± 0.012	1.809ProAsn: 1.809 ± 0.015	5.935ProPro: 5.935 ± 0.062	2.806ProGln: 2.806 ± 0.023	3.397ProArg: 3.397 ± 0.025	5.613ProSer: 5.613 ± 0.036	3.049ProThr: 3.049 ± 0.023	3.907ProVal: 3.907 ± 0.028	0.687ProTrp: 0.687 ± 0.008	1.554ProTyr: 1.554 ± 0.021	0.016ProXaa: 0.016 ± 0.001
Gln	3.424GlnAla: 3.424 ± 0.024	0.903GlnCys: 0.903 ± 0.011	2.289GlnAsp: 2.289 ± 0.015	3.82GlnGlu: 3.82 ± 0.026	1.338GlnPhe: 1.338 ± 0.011	2.831GlnGly: 2.831 ± 0.024	1.268GlnHis: 1.268 ± 0.013	2.008GlnIle: 2.008 ± 0.014	2.975GlnLys: 2.975 ± 0.02	4.647GlnLeu: 4.647 ± 0.03	1.128GlnMet: 1.128 ± 0.01	1.847GlnAsn: 1.847 ± 0.012	2.73GlnPro: 2.73 ± 0.025	2.913GlnGln: 2.913 ± 0.038	2.992GlnArg: 2.992 ± 0.023	3.087GlnSer: 3.087 ± 0.023	2.329GlnThr: 2.329 ± 0.015	2.785GlnVal: 2.785 ± 0.018	0.53GlnTrp: 0.53 ± 0.006	1.14GlnTyr: 1.14 ± 0.01	0.006GlnXaa: 0.006 ± 0.001
Arg	3.864ArgAla: 3.864 ± 0.026	1.193ArgCys: 1.193 ± 0.014	2.731ArgAsp: 2.731 ± 0.02	4.034ArgGlu: 4.034 ± 0.024	1.852ArgPhe: 1.852 ± 0.012	3.695ArgGly: 3.695 ± 0.031	1.547ArgHis: 1.547 ± 0.013	2.459ArgIle: 2.459 ± 0.018	3.708ArgLys: 3.708 ± 0.02	5.392ArgLeu: 5.392 ± 0.028	1.214ArgMet: 1.214 ± 0.01	2.145ArgAsn: 2.145 ± 0.014	3.308ArgPro: 3.308 ± 0.025	2.581ArgGln: 2.581 ± 0.017	4.336ArgArg: 4.336 ± 0.033	4.312ArgSer: 4.312 ± 0.036	2.863ArgThr: 2.863 ± 0.016	3.225ArgVal: 3.225 ± 0.026	0.686ArgTrp: 0.686 ± 0.008	1.485ArgTyr: 1.485 ± 0.011	0.013ArgXaa: 0.013 ± 0.001
Ser	5.248SerAla: 5.248 ± 0.027	1.837SerCys: 1.837 ± 0.02	3.921SerAsp: 3.921 ± 0.027	5.209SerGlu: 5.209 ± 0.03	3.095SerPhe: 3.095 ± 0.017	5.667SerGly: 5.667 ± 0.032	2.107SerHis: 2.107 ± 0.016	3.216SerIle: 3.216 ± 0.018	4.084SerLys: 4.084 ± 0.026	8.224SerLeu: 8.224 ± 0.039	1.586SerMet: 1.586 ± 0.011	2.669SerAsn: 2.669 ± 0.017	5.918SerPro: 5.918 ± 0.042	3.875SerGln: 3.875 ± 0.027	4.605SerArg: 4.605 ± 0.029	9.24SerSer: 9.24 ± 0.063	4.462SerThr: 4.462 ± 0.029	4.973SerVal: 4.973 ± 0.023	1.078SerTrp: 1.078 ± 0.011	2.094SerTyr: 2.094 ± 0.015	0.014SerXaa: 0.014 ± 0.001
Thr	3.74ThrAla: 3.74 ± 0.02	1.324ThrCys: 1.324 ± 0.015	2.488ThrAsp: 2.488 ± 0.016	3.563ThrGlu: 3.563 ± 0.024	2.159ThrPhe: 2.159 ± 0.015	3.641ThrGly: 3.641 ± 0.033	1.324ThrHis: 1.324 ± 0.013	2.429ThrIle: 2.429 ± 0.02	2.678ThrLys: 2.678 ± 0.028	5.329ThrLeu: 5.329 ± 0.022	1.112ThrMet: 1.112 ± 0.01	1.747ThrAsn: 1.747 ± 0.014	3.531ThrPro: 3.531 ± 0.026	2.262ThrGln: 2.262 ± 0.015	2.525ThrArg: 2.525 ± 0.016	4.603ThrSer: 4.603 ± 0.029	2.971ThrThr: 2.971 ± 0.036	3.973ThrVal: 3.973 ± 0.031	0.695ThrTrp: 0.695 ± 0.012	1.398ThrTyr: 1.398 ± 0.011	0.01ThrXaa: 0.01 ± 0.001
Val	4.308ValAla: 4.308 ± 0.021	1.435ValCys: 1.435 ± 0.013	2.93ValAsp: 2.93 ± 0.02	3.811ValGlu: 3.811 ± 0.032	2.468ValPhe: 2.468 ± 0.019	3.458ValGly: 3.458 ± 0.02	1.581ValHis: 1.581 ± 0.014	3.031ValIle: 3.031 ± 0.023	3.438ValLys: 3.438 ± 0.031	6.32ValLeu: 6.32 ± 0.027	1.357ValMet: 1.357 ± 0.012	2.278ValAsn: 2.278 ± 0.017	3.741ValPro: 3.741 ± 0.029	2.698ValGln: 2.698 ± 0.017	3.07ValArg: 3.07 ± 0.014	4.948ValSer: 4.948 ± 0.029	3.885ValThr: 3.885 ± 0.04	4.07ValVal: 4.07 ± 0.025	0.731ValTrp: 0.731 ± 0.008	1.607ValTyr: 1.607 ± 0.013	0.01ValXaa: 0.01 ± 0.001
Trp	0.778TrpAla: 0.778 ± 0.009	0.24TrpCys: 0.24 ± 0.004	0.652TrpAsp: 0.652 ± 0.008	0.797TrpGlu: 0.797 ± 0.009	0.45TrpPhe: 0.45 ± 0.008	0.734TrpGly: 0.734 ± 0.009	0.317TrpHis: 0.317 ± 0.005	0.546TrpIle: 0.546 ± 0.009	0.819TrpLys: 0.819 ± 0.01	1.201TrpLeu: 1.201 ± 0.012	0.305TrpMet: 0.305 ± 0.005	0.548TrpAsn: 0.548 ± 0.007	0.523TrpPro: 0.523 ± 0.007	0.525TrpGln: 0.525 ± 0.007	0.722TrpArg: 0.722 ± 0.008	0.857TrpSer: 0.857 ± 0.01	0.674TrpThr: 0.674 ± 0.011	0.7TrpVal: 0.7 ± 0.008	0.191TrpTrp: 0.191 ± 0.004	0.354TrpTyr: 0.354 ± 0.006	0.003TrpXaa: 0.003 ± 0.001
Tyr	1.395TyrAla: 1.395 ± 0.011	0.672TyrCys: 0.672 ± 0.007	1.302TyrAsp: 1.302 ± 0.012	1.717TyrGlu: 1.717 ± 0.015	1.269TyrPhe: 1.269 ± 0.012	1.695TyrGly: 1.695 ± 0.016	0.746TyrHis: 0.746 ± 0.007	1.413TyrIle: 1.413 ± 0.017	1.47TyrLys: 1.47 ± 0.02	2.636TyrLeu: 2.636 ± 0.018	0.607TyrMet: 0.607 ± 0.007	1.083TyrAsn: 1.083 ± 0.011	1.28TyrPro: 1.28 ± 0.013	1.238TyrGln: 1.238 ± 0.01	1.573TyrArg: 1.573 ± 0.012	2.189TyrSer: 2.189 ± 0.016	1.532TyrThr: 1.532 ± 0.016	1.644TyrVal: 1.644 ± 0.015	0.358TyrTrp: 0.358 ± 0.006	0.93TyrTyr: 0.93 ± 0.009	0.004TyrXaa: 0.004 ± 0.001
Xaa	0.013XaaAla: 0.013 ± 0.001	0.006XaaCys: 0.006 ± 0.001	0.006XaaAsp: 0.006 ± 0.001	0.009XaaGlu: 0.009 ± 0.001	0.007XaaPhe: 0.007 ± 0.001	0.016XaaGly: 0.016 ± 0.001	0.004XaaHis: 0.004 ± 0.001	0.005XaaIle: 0.005 ± 0.001	0.008XaaLys: 0.008 ± 0.001	0.014XaaLeu: 0.014 ± 0.001	0.003XaaMet: 0.003 ± 0.001	0.004XaaAsn: 0.004 ± 0.001	0.014XaaPro: 0.014 ± 0.001	0.006XaaGln: 0.006 ± 0.001	0.011XaaArg: 0.011 ± 0.001	0.013XaaSer: 0.013 ± 0.001	0.008XaaThr: 0.008 ± 0.001	0.012XaaVal: 0.012 ± 0.001	0.003XaaTrp: 0.003 ± 0.001	0.003XaaTyr: 0.003 ± 0.001	0.038XaaXaa: 0.038 ± 0.007

Statistics based on 22836 proteins (13162948 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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