Escherichia phage N15 (Bacteriophage N15)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Ravinvirus

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|O64327|O64327_BPN15 Gp13 OS=Escherichia phage N15 OX=40631 GN=gene 13 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 10.26HH3 pKa = 6.21RR4 pKa = 11.84DD5 pKa = 3.08IRR7 pKa = 11.84KK8 pKa = 9.41VIIDD12 pKa = 3.91ALEE15 pKa = 4.06SAIGTDD21 pKa = 4.07AIYY24 pKa = 10.63FDD26 pKa = 4.16GRR28 pKa = 11.84PAVLEE33 pKa = 4.45EE34 pKa = 4.3GDD36 pKa = 4.1FPAVAVYY43 pKa = 8.52LTDD46 pKa = 3.48AEE48 pKa = 4.64YY49 pKa = 10.08TGEE52 pKa = 4.28EE53 pKa = 4.3LDD55 pKa = 3.65ADD57 pKa = 3.87TWQAILHH64 pKa = 6.05IEE66 pKa = 4.24VFLEE70 pKa = 3.98AQVPDD75 pKa = 4.15SEE77 pKa = 5.39LDD79 pKa = 3.07DD80 pKa = 3.65WMEE83 pKa = 3.9TRR85 pKa = 11.84VYY87 pKa = 10.09PVLAEE92 pKa = 4.16VPGLEE97 pKa = 4.18SLITTMVQQGYY108 pKa = 10.04DD109 pKa = 3.56YY110 pKa = 11.36QRR112 pKa = 11.84DD113 pKa = 3.61DD114 pKa = 6.45DD115 pKa = 3.94MALWSSADD123 pKa = 3.42LKK125 pKa = 11.49YY126 pKa = 11.03SITYY130 pKa = 10.99DD131 pKa = 3.22MM132 pKa = 5.82

Molecular weight: 15.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9T0R7|Q9T0R7_BPN15 Gp6 OS=Escherichia phage N15 OX=40631 GN=gene 6 PE=4 SV=1
MM1 pKa = 7.67GPTSVGPVSSCTGVEE16 pKa = 4.07NPVWATTPIEE26 pKa = 4.01ILNSGGSTLYY36 pKa = 10.56KK37 pKa = 9.98IGMHH41 pKa = 5.53TMFKK45 pKa = 10.43FKK47 pKa = 10.09FAAVVRR53 pKa = 11.84TDD55 pKa = 3.4KK56 pKa = 10.73KK57 pKa = 10.17SHH59 pKa = 4.65IHH61 pKa = 6.13RR62 pKa = 11.84LSTIASSEE70 pKa = 4.06RR71 pKa = 11.84EE72 pKa = 3.83ARR74 pKa = 11.84RR75 pKa = 11.84QFASRR80 pKa = 11.84FVLVLSARR88 pKa = 11.84IPVSEE93 pKa = 4.66VIAA96 pKa = 4.27

Molecular weight: 10.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

14323

18

1324

238.7

26.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.865 ± 0.461

1.152 ± 0.154

6.011 ± 0.238

6.689 ± 0.35

3.254 ± 0.17

7.345 ± 0.34

1.501 ± 0.188

5.292 ± 0.183

5.173 ± 0.299

8.211 ± 0.315

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.618 ± 0.156

4.07 ± 0.193

4.119 ± 0.207

4.231 ± 0.296

6.207 ± 0.315

6.884 ± 0.242

6.011 ± 0.362

6.437 ± 0.23

1.711 ± 0.166

3.219 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski