Amino acid dipepetide frequency for Escherichia phage N15 (Bacteriophage N15)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.241AlaAla: 11.241 ± 1.323
1.047AlaCys: 1.047 ± 0.247
6.424AlaAsp: 6.424 ± 0.477
6.773AlaGlu: 6.773 ± 0.74
2.723AlaPhe: 2.723 ± 0.447
7.89AlaGly: 7.89 ± 0.839
1.327AlaHis: 1.327 ± 0.316
5.725AlaIle: 5.725 ± 0.569
4.538AlaLys: 4.538 ± 0.546
8.588AlaLeu: 8.588 ± 0.8
2.374AlaMet: 2.374 ± 0.356
3.561AlaAsn: 3.561 ± 0.573
2.863AlaPro: 2.863 ± 0.445
4.748AlaGln: 4.748 ± 0.839
6.005AlaArg: 6.005 ± 0.75
5.865AlaSer: 5.865 ± 0.699
5.097AlaThr: 5.097 ± 0.698
6.494AlaVal: 6.494 ± 0.641
2.234AlaTrp: 2.234 ± 0.358
3.142AlaTyr: 3.142 ± 0.459
0.0AlaXaa: 0.0 ± 0.0
Cys
0.908CysAla: 0.908 ± 0.24
0.14CysCys: 0.14 ± 0.094
0.838CysAsp: 0.838 ± 0.231
0.768CysGlu: 0.768 ± 0.233
0.419CysPhe: 0.419 ± 0.174
1.257CysGly: 1.257 ± 0.282
0.279CysHis: 0.279 ± 0.146
0.768CysIle: 0.768 ± 0.232
0.419CysLys: 0.419 ± 0.18
0.768CysLeu: 0.768 ± 0.243
0.209CysMet: 0.209 ± 0.113
0.419CysAsn: 0.419 ± 0.137
0.419CysPro: 0.419 ± 0.17
0.559CysGln: 0.559 ± 0.231
1.047CysArg: 1.047 ± 0.328
0.838CysSer: 0.838 ± 0.274
0.349CysThr: 0.349 ± 0.15
0.349CysVal: 0.349 ± 0.137
0.489CysTrp: 0.489 ± 0.178
0.279CysTyr: 0.279 ± 0.153
0.0CysXaa: 0.0 ± 0.0
Asp
5.935AspAla: 5.935 ± 0.583
0.838AspCys: 0.838 ± 0.257
4.538AspAsp: 4.538 ± 0.85
4.957AspGlu: 4.957 ± 0.817
2.234AspPhe: 2.234 ± 0.308
5.516AspGly: 5.516 ± 0.607
0.489AspHis: 0.489 ± 0.145
2.933AspIle: 2.933 ± 0.399
3.212AspLys: 3.212 ± 0.468
4.538AspLeu: 4.538 ± 0.741
1.466AspMet: 1.466 ± 0.316
1.606AspAsn: 1.606 ± 0.288
2.933AspPro: 2.933 ± 0.592
1.257AspGln: 1.257 ± 0.329
2.863AspArg: 2.863 ± 0.46
3.491AspSer: 3.491 ± 0.64
3.701AspThr: 3.701 ± 0.551
3.98AspVal: 3.98 ± 0.451
1.327AspTrp: 1.327 ± 0.328
2.304AspTyr: 2.304 ± 0.391
0.0AspXaa: 0.0 ± 0.0
Glu
6.075GluAla: 6.075 ± 0.741
0.978GluCys: 0.978 ± 0.251
2.793GluAsp: 2.793 ± 0.388
4.818GluGlu: 4.818 ± 0.676
2.304GluPhe: 2.304 ± 0.427
3.142GluGly: 3.142 ± 0.455
1.327GluHis: 1.327 ± 0.308
3.282GluIle: 3.282 ± 0.515
4.329GluLys: 4.329 ± 0.602
8.379GluLeu: 8.379 ± 0.748
1.885GluMet: 1.885 ± 0.366
2.723GluAsn: 2.723 ± 0.422
2.793GluPro: 2.793 ± 0.515
3.421GluGln: 3.421 ± 0.453
4.469GluArg: 4.469 ± 0.576
4.12GluSer: 4.12 ± 0.581
3.282GluThr: 3.282 ± 0.401
4.05GluVal: 4.05 ± 0.479
0.628GluTrp: 0.628 ± 0.253
2.095GluTyr: 2.095 ± 0.366
0.0GluXaa: 0.0 ± 0.0
Phe
3.002PheAla: 3.002 ± 0.444
0.698PheCys: 0.698 ± 0.249
2.653PheAsp: 2.653 ± 0.43
1.885PheGlu: 1.885 ± 0.404
1.047PhePhe: 1.047 ± 0.282
2.374PheGly: 2.374 ± 0.383
0.698PheHis: 0.698 ± 0.2
1.396PheIle: 1.396 ± 0.275
1.815PheLys: 1.815 ± 0.35
1.396PheLeu: 1.396 ± 0.294
1.187PheMet: 1.187 ± 0.251
1.606PheAsn: 1.606 ± 0.335
1.257PhePro: 1.257 ± 0.287
1.117PheGln: 1.117 ± 0.24
1.606PheArg: 1.606 ± 0.301
2.723PheSer: 2.723 ± 0.42
2.165PheThr: 2.165 ± 0.417
2.095PheVal: 2.095 ± 0.301
0.419PheTrp: 0.419 ± 0.135
1.396PheTyr: 1.396 ± 0.258
0.0PheXaa: 0.0 ± 0.0
Gly
5.307GlyAla: 5.307 ± 0.63
0.908GlyCys: 0.908 ± 0.289
5.446GlyAsp: 5.446 ± 0.648
4.189GlyGlu: 4.189 ± 0.679
2.514GlyPhe: 2.514 ± 0.429
5.376GlyGly: 5.376 ± 0.785
0.908GlyHis: 0.908 ± 0.225
4.957GlyIle: 4.957 ± 0.511
4.399GlyLys: 4.399 ± 0.542
4.888GlyLeu: 4.888 ± 0.492
2.095GlyMet: 2.095 ± 0.338
4.05GlyAsn: 4.05 ± 0.594
1.606GlyPro: 1.606 ± 0.367
3.072GlyGln: 3.072 ± 0.498
4.469GlyArg: 4.469 ± 0.414
5.376GlySer: 5.376 ± 0.686
4.748GlyThr: 4.748 ± 0.889
5.027GlyVal: 5.027 ± 0.635
1.257GlyTrp: 1.257 ± 0.319
2.863GlyTyr: 2.863 ± 0.406
0.0GlyXaa: 0.0 ± 0.0
His
1.117HisAla: 1.117 ± 0.3
0.209HisCys: 0.209 ± 0.113
1.327HisAsp: 1.327 ± 0.283
0.838HisGlu: 0.838 ± 0.231
0.838HisPhe: 0.838 ± 0.295
1.187HisGly: 1.187 ± 0.26
0.209HisHis: 0.209 ± 0.132
1.187HisIle: 1.187 ± 0.254
0.628HisLys: 0.628 ± 0.255
1.187HisLeu: 1.187 ± 0.29
0.14HisMet: 0.14 ± 0.092
0.489HisAsn: 0.489 ± 0.192
0.628HisPro: 0.628 ± 0.198
0.489HisGln: 0.489 ± 0.192
1.187HisArg: 1.187 ± 0.27
0.838HisSer: 0.838 ± 0.223
0.698HisThr: 0.698 ± 0.223
0.838HisVal: 0.838 ± 0.228
0.14HisTrp: 0.14 ± 0.103
0.838HisTyr: 0.838 ± 0.273
0.0HisXaa: 0.0 ± 0.0
Ile
4.748IleAla: 4.748 ± 0.537
0.838IleCys: 0.838 ± 0.229
3.561IleAsp: 3.561 ± 0.409
4.05IleGlu: 4.05 ± 0.482
1.327IlePhe: 1.327 ± 0.268
3.561IleGly: 3.561 ± 0.456
1.047IleHis: 1.047 ± 0.276
2.933IleIle: 2.933 ± 0.476
2.444IleLys: 2.444 ± 0.523
3.351IleLeu: 3.351 ± 0.506
1.396IleMet: 1.396 ± 0.345
2.583IleAsn: 2.583 ± 0.381
2.234IlePro: 2.234 ± 0.409
2.165IleGln: 2.165 ± 0.413
3.631IleArg: 3.631 ± 0.465
3.701IleSer: 3.701 ± 0.535
3.421IleThr: 3.421 ± 0.439
3.421IleVal: 3.421 ± 0.514
0.908IleTrp: 0.908 ± 0.268
1.606IleTyr: 1.606 ± 0.293
0.0IleXaa: 0.0 ± 0.0
Lys
4.329LysAla: 4.329 ± 0.459
0.628LysCys: 0.628 ± 0.234
2.095LysAsp: 2.095 ± 0.341
4.05LysGlu: 4.05 ± 0.602
1.327LysPhe: 1.327 ± 0.346
3.282LysGly: 3.282 ± 0.404
0.908LysHis: 0.908 ± 0.242
2.514LysIle: 2.514 ± 0.368
3.351LysLys: 3.351 ± 0.49
4.818LysLeu: 4.818 ± 0.776
1.606LysMet: 1.606 ± 0.384
2.234LysAsn: 2.234 ± 0.434
2.444LysPro: 2.444 ± 0.414
2.234LysGln: 2.234 ± 0.448
3.142LysArg: 3.142 ± 0.56
3.421LysSer: 3.421 ± 0.525
3.491LysThr: 3.491 ± 0.377
3.072LysVal: 3.072 ± 0.468
1.047LysTrp: 1.047 ± 0.244
1.746LysTyr: 1.746 ± 0.35
0.0LysXaa: 0.0 ± 0.0
Leu
8.099LeuAla: 8.099 ± 0.763
0.768LeuCys: 0.768 ± 0.235
5.237LeuAsp: 5.237 ± 0.472
5.097LeuGlu: 5.097 ± 0.583
2.374LeuPhe: 2.374 ± 0.352
4.608LeuGly: 4.608 ± 0.581
1.746LeuHis: 1.746 ± 0.363
3.351LeuIle: 3.351 ± 0.482
5.027LeuLys: 5.027 ± 0.657
6.424LeuLeu: 6.424 ± 0.75
1.606LeuMet: 1.606 ± 0.381
4.189LeuAsn: 4.189 ± 0.478
3.91LeuPro: 3.91 ± 0.514
4.818LeuGln: 4.818 ± 0.669
5.446LeuArg: 5.446 ± 0.704
6.005LeuSer: 6.005 ± 0.737
5.516LeuThr: 5.516 ± 0.667
4.818LeuVal: 4.818 ± 0.637
0.978LeuTrp: 0.978 ± 0.333
2.095LeuTyr: 2.095 ± 0.398
0.0LeuXaa: 0.0 ± 0.0
Met
2.723MetAla: 2.723 ± 0.411
0.07MetCys: 0.07 ± 0.07
0.838MetAsp: 0.838 ± 0.228
1.815MetGlu: 1.815 ± 0.353
0.978MetPhe: 0.978 ± 0.24
1.606MetGly: 1.606 ± 0.29
0.419MetHis: 0.419 ± 0.134
1.396MetIle: 1.396 ± 0.295
1.815MetLys: 1.815 ± 0.342
2.374MetLeu: 2.374 ± 0.377
0.628MetMet: 0.628 ± 0.187
0.838MetAsn: 0.838 ± 0.233
0.908MetPro: 0.908 ± 0.258
0.978MetGln: 0.978 ± 0.219
1.815MetArg: 1.815 ± 0.343
2.583MetSer: 2.583 ± 0.36
2.095MetThr: 2.095 ± 0.403
1.676MetVal: 1.676 ± 0.316
0.07MetTrp: 0.07 ± 0.056
0.559MetTyr: 0.559 ± 0.182
0.0MetXaa: 0.0 ± 0.0
Asn
4.189AsnAla: 4.189 ± 0.56
0.419AsnCys: 0.419 ± 0.177
2.514AsnAsp: 2.514 ± 0.527
3.002AsnGlu: 3.002 ± 0.529
0.908AsnPhe: 0.908 ± 0.222
4.12AsnGly: 4.12 ± 0.497
0.698AsnHis: 0.698 ± 0.157
2.444AsnIle: 2.444 ± 0.463
1.955AsnLys: 1.955 ± 0.336
1.885AsnLeu: 1.885 ± 0.318
1.047AsnMet: 1.047 ± 0.26
1.955AsnAsn: 1.955 ± 0.341
2.304AsnPro: 2.304 ± 0.356
1.396AsnGln: 1.396 ± 0.354
2.095AsnArg: 2.095 ± 0.373
2.444AsnSer: 2.444 ± 0.404
2.723AsnThr: 2.723 ± 0.328
1.885AsnVal: 1.885 ± 0.414
0.908AsnTrp: 0.908 ± 0.236
1.815AsnTyr: 1.815 ± 0.37
0.0AsnXaa: 0.0 ± 0.0
Pro
4.678ProAla: 4.678 ± 0.466
0.419ProCys: 0.419 ± 0.17
2.933ProAsp: 2.933 ± 0.473
2.444ProGlu: 2.444 ± 0.432
1.885ProPhe: 1.885 ± 0.344
3.701ProGly: 3.701 ± 0.527
0.628ProHis: 0.628 ± 0.233
1.257ProIle: 1.257 ± 0.327
1.676ProLys: 1.676 ± 0.365
2.863ProLeu: 2.863 ± 0.405
0.628ProMet: 0.628 ± 0.171
1.327ProAsn: 1.327 ± 0.277
1.466ProPro: 1.466 ± 0.372
1.466ProGln: 1.466 ± 0.357
1.606ProArg: 1.606 ± 0.397
3.421ProSer: 3.421 ± 0.548
2.793ProThr: 2.793 ± 0.44
4.469ProVal: 4.469 ± 0.495
0.559ProTrp: 0.559 ± 0.215
0.978ProTyr: 0.978 ± 0.234
0.0ProXaa: 0.0 ± 0.0
Gln
4.399GlnAla: 4.399 ± 0.685
0.279GlnCys: 0.279 ± 0.189
1.676GlnAsp: 1.676 ± 0.351
2.444GlnGlu: 2.444 ± 0.409
1.466GlnPhe: 1.466 ± 0.268
2.234GlnGly: 2.234 ± 0.391
0.419GlnHis: 0.419 ± 0.149
2.095GlnIle: 2.095 ± 0.526
2.025GlnLys: 2.025 ± 0.384
4.888GlnLeu: 4.888 ± 0.519
1.187GlnMet: 1.187 ± 0.301
1.606GlnAsn: 1.606 ± 0.33
2.025GlnPro: 2.025 ± 0.334
2.444GlnGln: 2.444 ± 0.562
3.002GlnArg: 3.002 ± 0.425
2.933GlnSer: 2.933 ± 0.531
2.304GlnThr: 2.304 ± 0.4
2.653GlnVal: 2.653 ± 0.388
0.838GlnTrp: 0.838 ± 0.248
1.396GlnTyr: 1.396 ± 0.434
0.0GlnXaa: 0.0 ± 0.0
Arg
5.446ArgAla: 5.446 ± 0.789
0.559ArgCys: 0.559 ± 0.207
3.212ArgAsp: 3.212 ± 0.476
4.12ArgGlu: 4.12 ± 0.612
1.746ArgPhe: 1.746 ± 0.336
4.259ArgGly: 4.259 ± 0.457
0.978ArgHis: 0.978 ± 0.276
4.608ArgIle: 4.608 ± 0.426
3.212ArgLys: 3.212 ± 0.404
5.865ArgLeu: 5.865 ± 0.605
2.304ArgMet: 2.304 ± 0.294
3.212ArgAsn: 3.212 ± 0.422
1.955ArgPro: 1.955 ± 0.265
2.095ArgGln: 2.095 ± 0.359
5.237ArgArg: 5.237 ± 0.766
2.444ArgSer: 2.444 ± 0.431
3.282ArgThr: 3.282 ± 0.371
4.189ArgVal: 4.189 ± 0.663
1.327ArgTrp: 1.327 ± 0.331
2.025ArgTyr: 2.025 ± 0.309
0.0ArgXaa: 0.0 ± 0.0
Ser
7.331SerAla: 7.331 ± 0.952
0.698SerCys: 0.698 ± 0.192
4.399SerAsp: 4.399 ± 0.654
3.631SerGlu: 3.631 ± 0.483
2.514SerPhe: 2.514 ± 0.392
7.262SerGly: 7.262 ± 0.714
1.257SerHis: 1.257 ± 0.307
3.142SerIle: 3.142 ± 0.514
2.653SerLys: 2.653 ± 0.442
5.027SerLeu: 5.027 ± 0.657
1.606SerMet: 1.606 ± 0.361
1.676SerAsn: 1.676 ± 0.307
2.863SerPro: 2.863 ± 0.406
2.583SerGln: 2.583 ± 0.325
4.608SerArg: 4.608 ± 0.469
5.935SerSer: 5.935 ± 0.682
3.98SerThr: 3.98 ± 0.552
4.888SerVal: 4.888 ± 0.462
1.117SerTrp: 1.117 ± 0.205
1.676SerTyr: 1.676 ± 0.32
0.0SerXaa: 0.0 ± 0.0
Thr
7.611ThrAla: 7.611 ± 0.864
0.768ThrCys: 0.768 ± 0.203
3.351ThrAsp: 3.351 ± 0.569
3.701ThrGlu: 3.701 ± 0.503
2.095ThrPhe: 2.095 ± 0.351
4.189ThrGly: 4.189 ± 0.573
0.489ThrHis: 0.489 ± 0.185
3.77ThrIle: 3.77 ± 0.502
1.536ThrLys: 1.536 ± 0.286
5.865ThrLeu: 5.865 ± 0.648
1.676ThrMet: 1.676 ± 0.373
2.025ThrAsn: 2.025 ± 0.356
2.863ThrPro: 2.863 ± 0.446
2.095ThrGln: 2.095 ± 0.374
4.05ThrArg: 4.05 ± 0.377
3.491ThrSer: 3.491 ± 0.368
3.631ThrThr: 3.631 ± 0.618
3.701ThrVal: 3.701 ± 0.592
1.257ThrTrp: 1.257 ± 0.232
1.955ThrTyr: 1.955 ± 0.427
0.0ThrXaa: 0.0 ± 0.0
Val
6.494ValAla: 6.494 ± 0.66
0.628ValCys: 0.628 ± 0.247
3.701ValAsp: 3.701 ± 0.469
5.446ValGlu: 5.446 ± 0.549
2.374ValPhe: 2.374 ± 0.335
4.259ValGly: 4.259 ± 0.579
0.419ValHis: 0.419 ± 0.152
3.351ValIle: 3.351 ± 0.463
4.05ValLys: 4.05 ± 0.572
4.469ValLeu: 4.469 ± 0.654
1.815ValMet: 1.815 ± 0.383
2.933ValAsn: 2.933 ± 0.463
2.723ValPro: 2.723 ± 0.458
2.234ValGln: 2.234 ± 0.437
3.002ValArg: 3.002 ± 0.453
5.446ValSer: 5.446 ± 0.711
3.91ValThr: 3.91 ± 0.52
3.91ValVal: 3.91 ± 0.512
1.047ValTrp: 1.047 ± 0.292
2.095ValTyr: 2.095 ± 0.472
0.0ValXaa: 0.0 ± 0.0
Trp
1.187TrpAla: 1.187 ± 0.236
0.279TrpCys: 0.279 ± 0.135
0.698TrpAsp: 0.698 ± 0.192
1.047TrpGlu: 1.047 ± 0.263
0.698TrpPhe: 0.698 ± 0.283
1.257TrpGly: 1.257 ± 0.322
0.279TrpHis: 0.279 ± 0.139
0.628TrpIle: 0.628 ± 0.241
0.628TrpLys: 0.628 ± 0.21
2.095TrpLeu: 2.095 ± 0.38
0.628TrpMet: 0.628 ± 0.231
0.628TrpAsn: 0.628 ± 0.258
1.047TrpPro: 1.047 ± 0.245
1.466TrpGln: 1.466 ± 0.3
1.187TrpArg: 1.187 ± 0.274
0.978TrpSer: 0.978 ± 0.235
0.698TrpThr: 0.698 ± 0.243
1.047TrpVal: 1.047 ± 0.267
0.279TrpTrp: 0.279 ± 0.134
0.349TrpTyr: 0.349 ± 0.127
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.84TyrAla: 3.84 ± 0.453
0.349TyrCys: 0.349 ± 0.154
1.885TyrAsp: 1.885 ± 0.363
1.815TyrGlu: 1.815 ± 0.33
0.768TyrPhe: 0.768 ± 0.239
2.374TyrGly: 2.374 ± 0.404
0.489TyrHis: 0.489 ± 0.175
1.187TyrIle: 1.187 ± 0.274
2.025TyrLys: 2.025 ± 0.516
2.444TyrLeu: 2.444 ± 0.446
0.628TyrMet: 0.628 ± 0.214
1.047TyrAsn: 1.047 ± 0.213
1.955TyrPro: 1.955 ± 0.375
1.676TyrGln: 1.676 ± 0.297
1.606TyrArg: 1.606 ± 0.293
2.793TyrSer: 2.793 ± 0.454
2.234TyrThr: 2.234 ± 0.445
1.815TyrVal: 1.815 ± 0.419
0.279TyrTrp: 0.279 ± 0.135
0.978TyrTyr: 0.978 ± 0.293
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 60 proteins (14323 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski