Jatrophihabitans endophyticus
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4181 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M5C0L1|A0A1M5C0L1_9ACTN Uncharacterized protein OS=Jatrophihabitans endophyticus OX=1206085 GN=SAMN05443575_0061 PE=4 SV=1
MM1 pKa = 7.38 NIWLKK6 pKa = 10.84 RR7 pKa = 11.84 SLQMGLLGAGVWLVGTGIAEE27 pKa = 4.74 ADD29 pKa = 3.74 TAPAVPPSQVSLAHH43 pKa = 6.19 VDD45 pKa = 2.83 VDD47 pKa = 4.05 VPVTVGHH54 pKa = 5.6 NHH56 pKa = 3.79 VTVANGSGDD65 pKa = 3.6 ASAHH69 pKa = 6.22 SDD71 pKa = 3.14 TSAKK75 pKa = 10.32 AGSSSGSSSRR85 pKa = 11.84 SSVVDD90 pKa = 3.37 VSVHH94 pKa = 4.96 VPVTVSDD101 pKa = 3.54 NDD103 pKa = 3.49 MAVADD108 pKa = 4.66 GSNSGGSNSGGSNSGGSNSGGSNSGGSNSGGSKK141 pKa = 9.55 DD142 pKa = 3.48 TDD144 pKa = 3.29 NGGSAKK150 pKa = 10.43 SGADD154 pKa = 3.08 NGGDD158 pKa = 3.41 EE159 pKa = 5.38 KK160 pKa = 11.45 GGADD164 pKa = 3.23 AKK166 pKa = 10.6 QRR168 pKa = 11.84 SLVSVPVSAPITVAGNAVGVLGDD191 pKa = 4.5 ADD193 pKa = 4.02 SSSTGAGSAGSGGASASDD211 pKa = 3.65 GAVEE215 pKa = 4.42 VPVNAPVTVCGNGVGVLGEE234 pKa = 4.22 GTADD238 pKa = 3.6 CGAGSTSSGASGDD251 pKa = 3.58 GSLVEE256 pKa = 4.43 VPVNAPVTVCGNGVGVAGDD275 pKa = 3.68 AAAGCGAGSAGSTSTGSGGASASDD299 pKa = 3.65 GAVEE303 pKa = 4.42 VPVNAPVTVCGNGVGVAGDD322 pKa = 3.68 AAAGCGAGSAGSTSTGSGGASASDD346 pKa = 3.75 GAVEE350 pKa = 4.42 APVNAPVTVCGNGVGVAGDD369 pKa = 3.68 AAAGCGAGSAGSTSTGSGGASASDD393 pKa = 3.65 GAVEE397 pKa = 4.42 VPVNAPVTVCGNGVGALGDD416 pKa = 3.85 ASADD420 pKa = 3.78 CASGSTGGATTGGSDD435 pKa = 3.44 GSTGGDD441 pKa = 3.38 DD442 pKa = 3.73 GSVAEE447 pKa = 4.7 VPVNAPVTVCGNGVGVAGDD466 pKa = 3.68 AAAGCGAGSTAGSSTGSGASTGNDD490 pKa = 2.84 GGVAEE495 pKa = 4.85 VPVNAPVTVCGNGVGALGDD514 pKa = 3.76 ASADD518 pKa = 3.78 CTSGSTGGATTGGSDD533 pKa = 3.44 GSTGGDD539 pKa = 3.38 DD540 pKa = 3.73 GSVAEE545 pKa = 4.7 VPVNAPVTVCGNGVGALGDD564 pKa = 3.76 ASADD568 pKa = 3.78 CTSGSTGGATTGGSDD583 pKa = 3.44 GSTGGDD589 pKa = 3.41 DD590 pKa = 4.12 TIVDD594 pKa = 4.01 VPVEE598 pKa = 4.26 VPVTVCGNAVGSADD612 pKa = 3.92 SSCSTTTTTGGGTDD626 pKa = 3.3 GGTDD630 pKa = 3.29 GGTVTVPVDD639 pKa = 3.63 TPVTVCGNGVGLLGTASASCSTGGDD664 pKa = 3.4 TPGTNDD670 pKa = 4.63 PGTNDD675 pKa = 4.35 PGSNDD680 pKa = 4.02 PGTNDD685 pKa = 4.44 PGSNDD690 pKa = 3.87 PGSDD694 pKa = 3.35 NPTVTDD700 pKa = 4.0 PVNAGSDD707 pKa = 3.73 TTVEE711 pKa = 4.39 AATSTPDD718 pKa = 3.01 SHH720 pKa = 9.44 SSDD723 pKa = 3.33 TVGGGRR729 pKa = 11.84 QLAQTGADD737 pKa = 3.28 TRR739 pKa = 11.84 GVLVGAVLLMLLGCVLGFAGRR760 pKa = 11.84 RR761 pKa = 11.84 RR762 pKa = 11.84 TNGG765 pKa = 2.91
Molecular weight: 68.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.788
IPC2_protein 3.465
IPC_protein 3.541
Toseland 3.287
ProMoST 3.732
Dawson 3.554
Bjellqvist 3.706
Wikipedia 3.528
Rodwell 3.35
Grimsley 3.185
Solomon 3.541
Lehninger 3.503
Nozaki 3.656
DTASelect 3.986
Thurlkill 3.35
EMBOSS 3.528
Sillero 3.668
Patrickios 0.54
IPC_peptide 3.541
IPC2_peptide 3.63
IPC2.peptide.svr19 3.629
Protein with the highest isoelectric point:
>tr|A0A1M5R2U3|A0A1M5R2U3_9ACTN YD repeat-containing protein OS=Jatrophihabitans endophyticus OX=1206085 GN=SAMN05443575_3423 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.99 KK16 pKa = 9.77 HH17 pKa = 5.81 RR18 pKa = 11.84 KK19 pKa = 8.51 LLKK22 pKa = 8.15 KK23 pKa = 9.25 TRR25 pKa = 11.84 VQRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.81 KK32 pKa = 9.63
Molecular weight: 4.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.473
IPC2_protein 11.082
IPC_protein 12.618
Toseland 12.808
ProMoST 13.276
Dawson 12.808
Bjellqvist 12.793
Wikipedia 13.276
Rodwell 12.735
Grimsley 12.837
Solomon 13.29
Lehninger 13.188
Nozaki 12.808
DTASelect 12.793
Thurlkill 12.808
EMBOSS 13.29
Sillero 12.808
Patrickios 12.457
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.03
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4181
0
4181
1383085
29
7281
330.8
35.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.472 ± 0.064
0.727 ± 0.009
6.523 ± 0.037
4.798 ± 0.042
2.693 ± 0.026
9.438 ± 0.04
2.165 ± 0.019
3.076 ± 0.027
1.697 ± 0.029
10.031 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.517 ± 0.014
1.717 ± 0.02
5.7 ± 0.029
2.634 ± 0.022
8.22 ± 0.051
5.117 ± 0.041
6.473 ± 0.048
9.652 ± 0.037
1.379 ± 0.014
1.971 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here