Amino acid dipepetide frequency for Nocardioides sp. HDW12B

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.978AlaAla: 18.978 ± 0.166	0.984AlaCys: 0.984 ± 0.028	8.572AlaAsp: 8.572 ± 0.095	8.307AlaGlu: 8.307 ± 0.093	3.529AlaPhe: 3.529 ± 0.069	12.517AlaGly: 12.517 ± 0.136	2.47AlaHis: 2.47 ± 0.052	3.562AlaIle: 3.562 ± 0.056	2.256AlaLys: 2.256 ± 0.059	13.5AlaLeu: 13.5 ± 0.12	2.564AlaMet: 2.564 ± 0.048	1.804AlaAsn: 1.804 ± 0.04	6.377AlaPro: 6.377 ± 0.083	3.386AlaGln: 3.386 ± 0.054	9.92AlaArg: 9.92 ± 0.092	6.474AlaSer: 6.474 ± 0.08	7.693AlaThr: 7.693 ± 0.078	12.043AlaVal: 12.043 ± 0.118	1.943AlaTrp: 1.943 ± 0.041	2.381AlaTyr: 2.381 ± 0.044	0.0AlaXaa: 0.0 ± 0.0
Cys	0.884CysAla: 0.884 ± 0.029	0.082CysCys: 0.082 ± 0.009	0.514CysAsp: 0.514 ± 0.019	0.401CysGlu: 0.401 ± 0.018	0.222CysPhe: 0.222 ± 0.013	0.842CysGly: 0.842 ± 0.024	0.174CysHis: 0.174 ± 0.012	0.182CysIle: 0.182 ± 0.013	0.101CysLys: 0.101 ± 0.01	0.649CysLeu: 0.649 ± 0.024	0.094CysMet: 0.094 ± 0.008	0.136CysAsn: 0.136 ± 0.011	0.43CysPro: 0.43 ± 0.021	0.16CysGln: 0.16 ± 0.012	0.599CysArg: 0.599 ± 0.024	0.433CysSer: 0.433 ± 0.018	0.449CysThr: 0.449 ± 0.02	0.634CysVal: 0.634 ± 0.022	0.112CysTrp: 0.112 ± 0.01	0.129CysTyr: 0.129 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	8.298AspAla: 8.298 ± 0.082	0.382AspCys: 0.382 ± 0.017	4.998AspAsp: 4.998 ± 0.083	4.648AspGlu: 4.648 ± 0.064	1.644AspPhe: 1.644 ± 0.037	6.71AspGly: 6.71 ± 0.086	1.562AspHis: 1.562 ± 0.037	1.635AspIle: 1.635 ± 0.035	1.005AspLys: 1.005 ± 0.031	7.866AspLeu: 7.866 ± 0.087	0.846AspMet: 0.846 ± 0.028	0.978AspAsn: 0.978 ± 0.029	4.934AspPro: 4.934 ± 0.078	1.718AspGln: 1.718 ± 0.034	5.372AspArg: 5.372 ± 0.074	2.545AspSer: 2.545 ± 0.043	3.131AspThr: 3.131 ± 0.053	6.513AspVal: 6.513 ± 0.078	0.996AspTrp: 0.996 ± 0.029	1.193AspTyr: 1.193 ± 0.03	0.0AspXaa: 0.0 ± 0.0
Glu	7.506GluAla: 7.506 ± 0.082	0.322GluCys: 0.322 ± 0.015	3.457GluAsp: 3.457 ± 0.058	3.676GluGlu: 3.676 ± 0.069	1.312GluPhe: 1.312 ± 0.031	4.679GluGly: 4.679 ± 0.065	1.7GluHis: 1.7 ± 0.043	2.19GluIle: 2.19 ± 0.043	1.381GluLys: 1.381 ± 0.038	6.444GluLeu: 6.444 ± 0.073	0.978GluMet: 0.978 ± 0.03	0.964GluAsn: 0.964 ± 0.029	3.523GluPro: 3.523 ± 0.05	2.434GluGln: 2.434 ± 0.052	5.615GluArg: 5.615 ± 0.082	2.797GluSer: 2.797 ± 0.049	3.231GluThr: 3.231 ± 0.05	6.059GluVal: 6.059 ± 0.078	0.73GluTrp: 0.73 ± 0.023	0.964GluTyr: 0.964 ± 0.028	0.0GluXaa: 0.0 ± 0.0
Phe	3.569PheAla: 3.569 ± 0.058	0.281PheCys: 0.281 ± 0.017	2.094PheAsp: 2.094 ± 0.047	1.583PheGlu: 1.583 ± 0.041	0.88PhePhe: 0.88 ± 0.035	2.895PheGly: 2.895 ± 0.057	0.559PheHis: 0.559 ± 0.024	0.684PheIle: 0.684 ± 0.026	0.442PheLys: 0.442 ± 0.019	2.642PheLeu: 2.642 ± 0.052	0.407PheMet: 0.407 ± 0.018	0.567PheAsn: 0.567 ± 0.024	1.264PhePro: 1.264 ± 0.034	0.626PheGln: 0.626 ± 0.022	1.74PheArg: 1.74 ± 0.045	1.474PheSer: 1.474 ± 0.036	1.846PheThr: 1.846 ± 0.047	2.571PheVal: 2.571 ± 0.05	0.415PheTrp: 0.415 ± 0.019	0.571PheTyr: 0.571 ± 0.022	0.0PheXaa: 0.0 ± 0.0
Gly	10.715GlyAla: 10.715 ± 0.114	0.787GlyCys: 0.787 ± 0.029	5.88GlyAsp: 5.88 ± 0.1	5.374GlyGlu: 5.374 ± 0.071	3.013GlyPhe: 3.013 ± 0.048	8.625GlyGly: 8.625 ± 0.121	2.118GlyHis: 2.118 ± 0.043	3.2GlyIle: 3.2 ± 0.062	1.874GlyLys: 1.874 ± 0.038	9.623GlyLeu: 9.623 ± 0.113	1.912GlyMet: 1.912 ± 0.04	1.579GlyAsn: 1.579 ± 0.041	5.065GlyPro: 5.065 ± 0.072	2.54GlyGln: 2.54 ± 0.052	7.645GlyArg: 7.645 ± 0.1	5.986GlySer: 5.986 ± 0.086	5.912GlyThr: 5.912 ± 0.08	8.471GlyVal: 8.471 ± 0.093	1.805GlyTrp: 1.805 ± 0.041	2.07GlyTyr: 2.07 ± 0.041	0.0GlyXaa: 0.0 ± 0.0
His	2.596HisAla: 2.596 ± 0.044	0.191HisCys: 0.191 ± 0.012	1.551HisAsp: 1.551 ± 0.043	1.305HisGlu: 1.305 ± 0.032	0.537HisPhe: 0.537 ± 0.021	2.255HisGly: 2.255 ± 0.043	0.701HisHis: 0.701 ± 0.025	0.429HisIle: 0.429 ± 0.019	0.299HisLys: 0.299 ± 0.018	2.488HisLeu: 2.488 ± 0.05	0.259HisMet: 0.259 ± 0.016	0.316HisAsn: 0.316 ± 0.015	1.574HisPro: 1.574 ± 0.039	0.619HisGln: 0.619 ± 0.023	1.905HisArg: 1.905 ± 0.042	0.815HisSer: 0.815 ± 0.028	1.011HisThr: 1.011 ± 0.028	2.113HisVal: 2.113 ± 0.045	0.301HisTrp: 0.301 ± 0.015	0.395HisTyr: 0.395 ± 0.015	0.0HisXaa: 0.0 ± 0.0
Ile	4.094IleAla: 4.094 ± 0.067	0.284IleCys: 0.284 ± 0.015	2.207IleAsp: 2.207 ± 0.043	2.038IleGlu: 2.038 ± 0.044	0.764IlePhe: 0.764 ± 0.028	3.238IleGly: 3.238 ± 0.062	0.531IleHis: 0.531 ± 0.022	0.899IleIle: 0.899 ± 0.033	0.614IleLys: 0.614 ± 0.023	2.233IleLeu: 2.233 ± 0.053	0.415IleMet: 0.415 ± 0.021	0.629IleAsn: 0.629 ± 0.024	1.528IlePro: 1.528 ± 0.038	0.622IleGln: 0.622 ± 0.023	1.948IleArg: 1.948 ± 0.043	1.625IleSer: 1.625 ± 0.042	1.87IleThr: 1.87 ± 0.042	2.764IleVal: 2.764 ± 0.055	0.33IleTrp: 0.33 ± 0.018	0.518IleTyr: 0.518 ± 0.024	0.0IleXaa: 0.0 ± 0.0
Lys	2.314LysAla: 2.314 ± 0.05	0.079LysCys: 0.079 ± 0.007	1.053LysAsp: 1.053 ± 0.033	0.954LysGlu: 0.954 ± 0.032	0.409LysPhe: 0.409 ± 0.02	1.494LysGly: 1.494 ± 0.038	0.41LysHis: 0.41 ± 0.02	0.68LysIle: 0.68 ± 0.025	0.688LysLys: 0.688 ± 0.037	1.638LysLeu: 1.638 ± 0.045	0.328LysMet: 0.328 ± 0.017	0.399LysAsn: 0.399 ± 0.019	1.078LysPro: 1.078 ± 0.032	0.649LysGln: 0.649 ± 0.024	1.408LysArg: 1.408 ± 0.033	0.94LysSer: 0.94 ± 0.03	1.032LysThr: 1.032 ± 0.026	1.838LysVal: 1.838 ± 0.045	0.185LysTrp: 0.185 ± 0.011	0.391LysTyr: 0.391 ± 0.018	0.0LysXaa: 0.0 ± 0.0
Leu	15.013LeuAla: 15.013 ± 0.133	0.71LeuCys: 0.71 ± 0.022	7.482LeuAsp: 7.482 ± 0.091	5.822LeuGlu: 5.822 ± 0.07	2.377LeuPhe: 2.377 ± 0.051	9.794LeuGly: 9.794 ± 0.098	2.088LeuHis: 2.088 ± 0.043	2.498LeuIle: 2.498 ± 0.06	1.622LeuLys: 1.622 ± 0.042	10.979LeuLeu: 10.979 ± 0.129	1.581LeuMet: 1.581 ± 0.036	1.494LeuAsn: 1.494 ± 0.036	5.794LeuPro: 5.794 ± 0.077	2.389LeuGln: 2.389 ± 0.046	7.907LeuArg: 7.907 ± 0.095	5.331LeuSer: 5.331 ± 0.065	6.593LeuThr: 6.593 ± 0.076	10.91LeuVal: 10.91 ± 0.116	1.181LeuTrp: 1.181 ± 0.034	1.481LeuTyr: 1.481 ± 0.036	0.0LeuXaa: 0.0 ± 0.0
Met	2.157MetAla: 2.157 ± 0.039	0.125MetCys: 0.125 ± 0.009	0.857MetAsp: 0.857 ± 0.026	0.761MetGlu: 0.761 ± 0.021	0.464MetPhe: 0.464 ± 0.022	1.339MetGly: 1.339 ± 0.033	0.36MetHis: 0.36 ± 0.017	0.615MetIle: 0.615 ± 0.024	0.43MetLys: 0.43 ± 0.019	1.653MetLeu: 1.653 ± 0.04	0.304MetMet: 0.304 ± 0.015	0.394MetAsn: 0.394 ± 0.018	1.092MetPro: 1.092 ± 0.029	0.466MetGln: 0.466 ± 0.021	1.42MetArg: 1.42 ± 0.035	1.458MetSer: 1.458 ± 0.034	1.677MetThr: 1.677 ± 0.038	1.528MetVal: 1.528 ± 0.036	0.189MetTrp: 0.189 ± 0.013	0.262MetTyr: 0.262 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	1.997AsnAla: 1.997 ± 0.043	0.121AsnCys: 0.121 ± 0.009	1.061AsnAsp: 1.061 ± 0.031	0.817AsnGlu: 0.817 ± 0.027	0.446AsnPhe: 0.446 ± 0.021	1.634AsnGly: 1.634 ± 0.041	0.378AsnHis: 0.378 ± 0.019	0.594AsnIle: 0.594 ± 0.024	0.36AsnLys: 0.36 ± 0.016	1.717AsnLeu: 1.717 ± 0.041	0.252AsnMet: 0.252 ± 0.013	0.36AsnAsn: 0.36 ± 0.019	1.258AsnPro: 1.258 ± 0.033	0.493AsnGln: 0.493 ± 0.024	1.235AsnArg: 1.235 ± 0.031	0.7AsnSer: 0.7 ± 0.023	0.918AsnThr: 0.918 ± 0.025	1.445AsnVal: 1.445 ± 0.034	0.204AsnTrp: 0.204 ± 0.013	0.35AsnTyr: 0.35 ± 0.02	0.0AsnXaa: 0.0 ± 0.0
Pro	7.413ProAla: 7.413 ± 0.096	0.286ProCys: 0.286 ± 0.018	4.818ProAsp: 4.818 ± 0.075	4.103ProGlu: 4.103 ± 0.057	1.524ProPhe: 1.524 ± 0.033	6.19ProGly: 6.19 ± 0.08	1.168ProHis: 1.168 ± 0.033	1.354ProIle: 1.354 ± 0.035	0.883ProLys: 0.883 ± 0.031	4.957ProLeu: 4.957 ± 0.066	0.998ProMet: 0.998 ± 0.029	0.798ProAsn: 0.798 ± 0.025	3.161ProPro: 3.161 ± 0.074	1.514ProGln: 1.514 ± 0.036	3.947ProArg: 3.947 ± 0.058	3.275ProSer: 3.275 ± 0.062	3.678ProThr: 3.678 ± 0.061	5.431ProVal: 5.431 ± 0.068	0.895ProTrp: 0.895 ± 0.027	1.084ProTyr: 1.084 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	3.469GlnAla: 3.469 ± 0.053	0.153GlnCys: 0.153 ± 0.01	1.363GlnAsp: 1.363 ± 0.035	1.487GlnGlu: 1.487 ± 0.037	0.608GlnPhe: 0.608 ± 0.022	2.272GlnGly: 2.272 ± 0.044	0.616GlnHis: 0.616 ± 0.022	0.965GlnIle: 0.965 ± 0.031	0.501GlnLys: 0.501 ± 0.02	2.788GlnLeu: 2.788 ± 0.047	0.56GlnMet: 0.56 ± 0.022	0.414GlnAsn: 0.414 ± 0.019	1.505GlnPro: 1.505 ± 0.042	1.135GlnGln: 1.135 ± 0.036	2.449GlnArg: 2.449 ± 0.054	1.207GlnSer: 1.207 ± 0.033	1.352GlnThr: 1.352 ± 0.033	2.965GlnVal: 2.965 ± 0.05	0.406GlnTrp: 0.406 ± 0.017	0.473GlnTyr: 0.473 ± 0.023	0.0GlnXaa: 0.0 ± 0.0
Arg	9.24ArgAla: 9.24 ± 0.118	0.588ArgCys: 0.588 ± 0.022	4.853ArgAsp: 4.853 ± 0.065	4.757ArgGlu: 4.757 ± 0.058	2.321ArgPhe: 2.321 ± 0.049	6.182ArgGly: 6.182 ± 0.078	1.942ArgHis: 1.942 ± 0.039	2.696ArgIle: 2.696 ± 0.052	1.332ArgLys: 1.332 ± 0.033	8.617ArgLeu: 8.617 ± 0.1	1.739ArgMet: 1.739 ± 0.03	1.23ArgAsn: 1.23 ± 0.032	4.7ArgPro: 4.7 ± 0.077	2.052ArgGln: 2.052 ± 0.037	8.026ArgArg: 8.026 ± 0.105	4.481ArgSer: 4.481 ± 0.07	5.045ArgThr: 5.045 ± 0.056	6.872ArgVal: 6.872 ± 0.082	1.374ArgTrp: 1.374 ± 0.034	1.511ArgTyr: 1.511 ± 0.034	0.0ArgXaa: 0.0 ± 0.0
Ser	6.448SerAla: 6.448 ± 0.067	0.356SerCys: 0.356 ± 0.017	3.296SerAsp: 3.296 ± 0.055	2.888SerGlu: 2.888 ± 0.051	1.67SerPhe: 1.67 ± 0.042	5.916SerGly: 5.916 ± 0.08	1.034SerHis: 1.034 ± 0.031	1.559SerIle: 1.559 ± 0.037	0.943SerLys: 0.943 ± 0.028	5.129SerLeu: 5.129 ± 0.061	1.166SerMet: 1.166 ± 0.031	0.916SerAsn: 0.916 ± 0.029	3.129SerPro: 3.129 ± 0.051	1.3SerGln: 1.3 ± 0.033	3.979SerArg: 3.979 ± 0.055	3.396SerSer: 3.396 ± 0.066	3.512SerThr: 3.512 ± 0.064	4.642SerVal: 4.642 ± 0.056	0.972SerTrp: 0.972 ± 0.032	1.251SerTyr: 1.251 ± 0.034	0.0SerXaa: 0.0 ± 0.0
Thr	7.431ThrAla: 7.431 ± 0.088	0.488ThrCys: 0.488 ± 0.022	3.797ThrAsp: 3.797 ± 0.071	3.285ThrGlu: 3.285 ± 0.056	1.851ThrPhe: 1.851 ± 0.039	6.389ThrGly: 6.389 ± 0.077	1.164ThrHis: 1.164 ± 0.033	1.813ThrIle: 1.813 ± 0.044	1.04ThrLys: 1.04 ± 0.031	5.97ThrLeu: 5.97 ± 0.085	1.041ThrMet: 1.041 ± 0.027	1.017ThrAsn: 1.017 ± 0.034	4.005ThrPro: 4.005 ± 0.066	1.446ThrGln: 1.446 ± 0.031	4.227ThrArg: 4.227 ± 0.061	3.795ThrSer: 3.795 ± 0.058	4.248ThrThr: 4.248 ± 0.065	6.061ThrVal: 6.061 ± 0.074	1.026ThrTrp: 1.026 ± 0.031	1.416ThrTyr: 1.416 ± 0.03	0.0ThrXaa: 0.0 ± 0.0
Val	13.11ValAla: 13.11 ± 0.132	0.719ValCys: 0.719 ± 0.028	6.659ValAsp: 6.659 ± 0.077	6.043ValGlu: 6.043 ± 0.079	2.431ValPhe: 2.431 ± 0.042	8.244ValGly: 8.244 ± 0.087	2.034ValHis: 2.034 ± 0.047	2.734ValIle: 2.734 ± 0.052	1.6ValLys: 1.6 ± 0.039	10.435ValLeu: 10.435 ± 0.103	1.619ValMet: 1.619 ± 0.036	1.697ValAsn: 1.697 ± 0.038	5.431ValPro: 5.431 ± 0.068	2.1ValGln: 2.1 ± 0.04	7.344ValArg: 7.344 ± 0.09	4.849ValSer: 4.849 ± 0.069	6.355ValThr: 6.355 ± 0.069	11.286ValVal: 11.286 ± 0.127	1.162ValTrp: 1.162 ± 0.034	1.499ValTyr: 1.499 ± 0.033	0.0ValXaa: 0.0 ± 0.0
Trp	1.546TrpAla: 1.546 ± 0.037	0.139TrpCys: 0.139 ± 0.01	0.87TrpAsp: 0.87 ± 0.024	0.689TrpGlu: 0.689 ± 0.023	0.507TrpPhe: 0.507 ± 0.025	1.07TrpGly: 1.07 ± 0.031	0.336TrpHis: 0.336 ± 0.016	0.466TrpIle: 0.466 ± 0.017	0.287TrpLys: 0.287 ± 0.016	1.701TrpLeu: 1.701 ± 0.04	0.302TrpMet: 0.302 ± 0.016	0.317TrpAsn: 0.317 ± 0.018	0.719TrpPro: 0.719 ± 0.024	0.543TrpGln: 0.543 ± 0.023	1.38TrpArg: 1.38 ± 0.034	1.056TrpSer: 1.056 ± 0.031	0.984TrpThr: 0.984 ± 0.03	1.298TrpVal: 1.298 ± 0.036	0.354TrpTrp: 0.354 ± 0.017	0.291TrpTyr: 0.291 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.482TyrAla: 2.482 ± 0.052	0.148TyrCys: 0.148 ± 0.01	1.59TyrAsp: 1.59 ± 0.032	1.023TyrGlu: 1.023 ± 0.029	0.602TyrPhe: 0.602 ± 0.022	1.909TyrGly: 1.909 ± 0.041	0.318TyrHis: 0.318 ± 0.016	0.398TyrIle: 0.398 ± 0.017	0.31TyrLys: 0.31 ± 0.017	2.015TyrLeu: 2.015 ± 0.043	0.185TyrMet: 0.185 ± 0.011	0.333TyrAsn: 0.333 ± 0.015	0.909TyrPro: 0.909 ± 0.029	0.494TyrGln: 0.494 ± 0.02	1.494TyrArg: 1.494 ± 0.042	0.866TyrSer: 0.866 ± 0.029	0.973TyrThr: 0.973 ± 0.03	1.908TyrVal: 1.908 ± 0.038	0.275TyrTrp: 0.275 ± 0.015	0.377TyrTyr: 0.377 ± 0.019	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3834 proteins (1259540 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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