Achromobacter sp. DMS1
Average proteome isoelectric point is 7.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3366 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0L8EN01|A0A0L8EN01_9BURK Uncharacterized protein OS=Achromobacter sp. DMS1 OX=1688405 GN=AD428_18105 PE=4 SV=1
MM1 pKa = 6.51 THH3 pKa = 6.28 VVTEE7 pKa = 3.94 NCIKK11 pKa = 10.53 CKK13 pKa = 10.04 YY14 pKa = 7.62 TDD16 pKa = 4.08 CVDD19 pKa = 3.61 VCPVDD24 pKa = 4.18 CFRR27 pKa = 11.84 EE28 pKa = 4.9 GPNFLVIDD36 pKa = 4.26 PDD38 pKa = 3.65 EE39 pKa = 5.79 CIDD42 pKa = 3.87 CAVCIPEE49 pKa = 4.56 CPANAIYY56 pKa = 10.74 AEE58 pKa = 4.15 EE59 pKa = 5.28 DD60 pKa = 3.67 VPQDD64 pKa = 3.28 QLKK67 pKa = 10.55 FIAINAEE74 pKa = 4.07 LSPEE78 pKa = 3.93 FPTISRR84 pKa = 11.84 AKK86 pKa = 10.34 KK87 pKa = 8.84 PLPDD91 pKa = 4.03 ADD93 pKa = 3.64 EE94 pKa = 4.39 WNGVQDD100 pKa = 3.49 KK101 pKa = 10.62 LQYY104 pKa = 10.41 LEE106 pKa = 4.29 KK107 pKa = 10.85
Molecular weight: 12.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.921
IPC2_protein 4.151
IPC_protein 4.088
Toseland 3.897
ProMoST 4.228
Dawson 4.062
Bjellqvist 4.215
Wikipedia 3.961
Rodwell 3.91
Grimsley 3.808
Solomon 4.05
Lehninger 3.999
Nozaki 4.177
DTASelect 4.368
Thurlkill 3.935
EMBOSS 3.973
Sillero 4.202
Patrickios 0.744
IPC_peptide 4.05
IPC2_peptide 4.177
IPC2.peptide.svr19 4.091
Protein with the highest isoelectric point:
>tr|A0A0L8EKP6|A0A0L8EKP6_9BURK ATP-dependent DNA helicase (Fragment) OS=Achromobacter sp. DMS1 OX=1688405 GN=AD428_23260 PE=4 SV=1
MM1 pKa = 7.37 TMPRR5 pKa = 11.84 TRR7 pKa = 11.84 RR8 pKa = 11.84 PAPLRR13 pKa = 11.84 RR14 pKa = 11.84 AWIRR18 pKa = 11.84 SRR20 pKa = 11.84 PGRR23 pKa = 11.84 GRR25 pKa = 11.84 PGGGPGRR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 TRR37 pKa = 11.84 WWPTWKK43 pKa = 10.33 SSPPPPTPLARR54 pKa = 11.84 PRR56 pKa = 11.84 IAALLPSRR64 pKa = 11.84 HH65 pKa = 4.88 WAVAARR71 pKa = 11.84 RR72 pKa = 11.84 AGCRR76 pKa = 11.84 AGRR79 pKa = 11.84 RR80 pKa = 3.66
Molecular weight: 9.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.521
IPC2_protein 11.228
IPC_protein 12.837
Toseland 12.998
ProMoST 13.495
Dawson 12.998
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.515
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.495
Sillero 12.998
Patrickios 12.237
IPC_peptide 13.51
IPC2_peptide 12.501
IPC2.peptide.svr19 9.217
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3366
0
3366
969022
29
1757
287.9
31.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.788 ± 0.051
0.939 ± 0.014
5.232 ± 0.033
5.329 ± 0.038
3.532 ± 0.028
8.513 ± 0.041
2.17 ± 0.022
4.504 ± 0.034
3.024 ± 0.034
10.652 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.555 ± 0.021
2.568 ± 0.024
5.383 ± 0.029
3.752 ± 0.032
7.508 ± 0.047
5.219 ± 0.029
4.794 ± 0.031
7.586 ± 0.035
1.394 ± 0.018
2.558 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here