Amino acid dipepetide frequency for Achromobacter sp. DMS1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.277AlaAla: 18.277 ± 0.222	1.422AlaCys: 1.422 ± 0.041	6.542AlaAsp: 6.542 ± 0.083	6.646AlaGlu: 6.646 ± 0.09	4.14AlaPhe: 4.14 ± 0.078	11.606AlaGly: 11.606 ± 0.129	2.618AlaHis: 2.618 ± 0.059	5.316AlaIle: 5.316 ± 0.079	3.093AlaLys: 3.093 ± 0.073	14.55AlaLeu: 14.55 ± 0.158	3.623AlaMet: 3.623 ± 0.06	2.704AlaAsn: 2.704 ± 0.054	6.211AlaPro: 6.211 ± 0.098	5.426AlaGln: 5.426 ± 0.083	10.464AlaArg: 10.464 ± 0.131	6.326AlaSer: 6.326 ± 0.07	5.231AlaThr: 5.231 ± 0.075	9.008AlaVal: 9.008 ± 0.111	1.793AlaTrp: 1.793 ± 0.048	2.88AlaTyr: 2.88 ± 0.048	0.0AlaXaa: 0.0 ± 0.0
Cys	1.25CysAla: 1.25 ± 0.04	0.144CysCys: 0.144 ± 0.013	0.52CysAsp: 0.52 ± 0.025	0.458CysGlu: 0.458 ± 0.025	0.327CysPhe: 0.327 ± 0.021	1.013CysGly: 1.013 ± 0.032	0.264CysHis: 0.264 ± 0.02	0.381CysIle: 0.381 ± 0.02	0.209CysLys: 0.209 ± 0.016	0.905CysLeu: 0.905 ± 0.031	0.23CysMet: 0.23 ± 0.015	0.24CysAsn: 0.24 ± 0.017	0.496CysPro: 0.496 ± 0.022	0.265CysGln: 0.265 ± 0.014	0.667CysArg: 0.667 ± 0.027	0.477CysSer: 0.477 ± 0.022	0.402CysThr: 0.402 ± 0.021	0.776CysVal: 0.776 ± 0.032	0.139CysTrp: 0.139 ± 0.012	0.22CysTyr: 0.22 ± 0.018	0.001CysXaa: 0.001 ± 0.001
Asp	6.736AspAla: 6.736 ± 0.098	0.475AspCys: 0.475 ± 0.022	2.649AspAsp: 2.649 ± 0.055	3.194AspGlu: 3.194 ± 0.056	2.038AspPhe: 2.038 ± 0.051	4.667AspGly: 4.667 ± 0.084	1.051AspHis: 1.051 ± 0.032	2.617AspIle: 2.617 ± 0.053	1.537AspLys: 1.537 ± 0.043	5.44AspLeu: 5.44 ± 0.087	1.237AspMet: 1.237 ± 0.037	1.199AspAsn: 1.199 ± 0.039	3.262AspPro: 3.262 ± 0.059	1.579AspGln: 1.579 ± 0.043	3.495AspArg: 3.495 ± 0.064	2.112AspSer: 2.112 ± 0.045	2.592AspThr: 2.592 ± 0.063	3.997AspVal: 3.997 ± 0.064	0.886AspTrp: 0.886 ± 0.034	1.555AspTyr: 1.555 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	6.595GluAla: 6.595 ± 0.086	0.388GluCys: 0.388 ± 0.019	2.449GluAsp: 2.449 ± 0.047	2.65GluGlu: 2.65 ± 0.057	1.849GluPhe: 1.849 ± 0.048	3.841GluGly: 3.841 ± 0.064	1.382GluHis: 1.382 ± 0.034	2.826GluIle: 2.826 ± 0.065	1.902GluLys: 1.902 ± 0.054	5.734GluLeu: 5.734 ± 0.089	1.259GluMet: 1.259 ± 0.039	1.513GluAsn: 1.513 ± 0.043	2.679GluPro: 2.679 ± 0.057	2.419GluGln: 2.419 ± 0.052	4.74GluArg: 4.74 ± 0.072	2.63GluSer: 2.63 ± 0.061	2.69GluThr: 2.69 ± 0.058	3.792GluVal: 3.792 ± 0.068	0.69GluTrp: 0.69 ± 0.026	1.257GluTyr: 1.257 ± 0.04	0.001GluXaa: 0.001 ± 0.001
Phe	4.096PheAla: 4.096 ± 0.062	0.367PheCys: 0.367 ± 0.019	2.311PheAsp: 2.311 ± 0.054	1.961PheGlu: 1.961 ± 0.048	1.398PhePhe: 1.398 ± 0.046	3.405PheGly: 3.405 ± 0.067	0.755PheHis: 0.755 ± 0.032	1.572PheIle: 1.572 ± 0.048	1.089PheLys: 1.089 ± 0.034	3.274PheLeu: 3.274 ± 0.079	0.884PheMet: 0.884 ± 0.033	1.063PheAsn: 1.063 ± 0.036	1.617PhePro: 1.617 ± 0.04	1.083PheGln: 1.083 ± 0.03	2.213PheArg: 2.213 ± 0.053	2.19PheSer: 2.19 ± 0.049	1.8PheThr: 1.8 ± 0.043	2.706PheVal: 2.706 ± 0.061	0.554PheTrp: 0.554 ± 0.029	0.983PheTyr: 0.983 ± 0.034	0.0PheXaa: 0.0 ± 0.0
Gly	9.688GlyAla: 9.688 ± 0.115	0.872GlyCys: 0.872 ± 0.035	3.731GlyAsp: 3.731 ± 0.06	4.475GlyGlu: 4.475 ± 0.076	3.298GlyPhe: 3.298 ± 0.06	7.104GlyGly: 7.104 ± 0.115	1.899GlyHis: 1.899 ± 0.051	4.266GlyIle: 4.266 ± 0.086	3.498GlyLys: 3.498 ± 0.065	9.311GlyLeu: 9.311 ± 0.126	2.669GlyMet: 2.669 ± 0.054	2.196GlyAsn: 2.196 ± 0.053	3.357GlyPro: 3.357 ± 0.065	3.46GlyGln: 3.46 ± 0.064	6.102GlyArg: 6.102 ± 0.088	4.492GlySer: 4.492 ± 0.074	4.122GlyThr: 4.122 ± 0.075	6.562GlyVal: 6.562 ± 0.1	1.39GlyTrp: 1.39 ± 0.038	2.637GlyTyr: 2.637 ± 0.057	0.001GlyXaa: 0.001 ± 0.001
His	2.884HisAla: 2.884 ± 0.062	0.262HisCys: 0.262 ± 0.017	1.163HisAsp: 1.163 ± 0.041	1.095HisGlu: 1.095 ± 0.033	0.803HisPhe: 0.803 ± 0.029	2.093HisGly: 2.093 ± 0.05	0.553HisHis: 0.553 ± 0.027	0.973HisIle: 0.973 ± 0.03	0.501HisLys: 0.501 ± 0.027	2.16HisLeu: 2.16 ± 0.047	0.526HisMet: 0.526 ± 0.025	0.524HisAsn: 0.524 ± 0.023	1.519HisPro: 1.519 ± 0.045	0.724HisGln: 0.724 ± 0.025	1.438HisArg: 1.438 ± 0.038	0.938HisSer: 0.938 ± 0.032	1.059HisThr: 1.059 ± 0.034	1.521HisVal: 1.521 ± 0.038	0.35HisTrp: 0.35 ± 0.017	0.611HisTyr: 0.611 ± 0.028	0.001HisXaa: 0.001 ± 0.001
Ile	6.028IleAla: 6.028 ± 0.093	0.435IleCys: 0.435 ± 0.023	2.923IleAsp: 2.923 ± 0.057	3.037IleGlu: 3.037 ± 0.063	1.377IlePhe: 1.377 ± 0.043	4.312IleGly: 4.312 ± 0.075	0.847IleHis: 0.847 ± 0.032	1.784IleIle: 1.784 ± 0.051	1.419IleLys: 1.419 ± 0.042	3.852IleLeu: 3.852 ± 0.066	0.927IleMet: 0.927 ± 0.032	1.352IleAsn: 1.352 ± 0.046	2.224IlePro: 2.224 ± 0.049	1.324IleGln: 1.324 ± 0.033	2.896IleArg: 2.896 ± 0.053	2.514IleSer: 2.514 ± 0.058	2.316IleThr: 2.316 ± 0.045	3.835IleVal: 3.835 ± 0.069	0.557IleTrp: 0.557 ± 0.025	1.08IleTyr: 1.08 ± 0.032	0.0IleXaa: 0.0 ± 0.0
Lys	3.43LysAla: 3.43 ± 0.071	0.152LysCys: 0.152 ± 0.014	1.472LysAsp: 1.472 ± 0.041	1.448LysGlu: 1.448 ± 0.046	0.911LysPhe: 0.911 ± 0.036	2.244LysGly: 2.244 ± 0.041	0.602LysHis: 0.602 ± 0.025	1.394LysIle: 1.394 ± 0.044	1.235LysLys: 1.235 ± 0.043	3.277LysLeu: 3.277 ± 0.071	0.761LysMet: 0.761 ± 0.033	0.903LysAsn: 0.903 ± 0.033	1.961LysPro: 1.961 ± 0.044	1.224LysGln: 1.224 ± 0.035	2.185LysArg: 2.185 ± 0.06	1.706LysSer: 1.706 ± 0.048	1.827LysThr: 1.827 ± 0.045	2.396LysVal: 2.396 ± 0.058	0.404LysTrp: 0.404 ± 0.024	0.709LysTyr: 0.709 ± 0.032	0.0LysXaa: 0.0 ± 0.0
Leu	15.488LeuAla: 15.488 ± 0.176	1.088LeuCys: 1.088 ± 0.032	6.137LeuAsp: 6.137 ± 0.087	5.289LeuGlu: 5.289 ± 0.079	3.623LeuPhe: 3.623 ± 0.062	8.984LeuGly: 8.984 ± 0.109	2.167LeuHis: 2.167 ± 0.058	4.516LeuIle: 4.516 ± 0.082	3.258LeuLys: 3.258 ± 0.069	10.691LeuLeu: 10.691 ± 0.157	2.452LeuMet: 2.452 ± 0.056	2.694LeuAsn: 2.694 ± 0.055	6.04LeuPro: 6.04 ± 0.096	3.413LeuGln: 3.413 ± 0.066	8.176LeuArg: 8.176 ± 0.117	6.238LeuSer: 6.238 ± 0.094	5.094LeuThr: 5.094 ± 0.073	7.702LeuVal: 7.702 ± 0.101	1.132LeuTrp: 1.132 ± 0.035	2.337LeuTyr: 2.337 ± 0.053	0.001LeuXaa: 0.001 ± 0.001
Met	3.16MetAla: 3.16 ± 0.062	0.2MetCys: 0.2 ± 0.014	1.159MetAsp: 1.159 ± 0.031	1.087MetGlu: 1.087 ± 0.035	0.793MetPhe: 0.793 ± 0.028	1.869MetGly: 1.869 ± 0.043	0.56MetHis: 0.56 ± 0.024	1.096MetIle: 1.096 ± 0.041	0.982MetLys: 0.982 ± 0.033	2.915MetLeu: 2.915 ± 0.051	0.642MetMet: 0.642 ± 0.022	0.856MetAsn: 0.856 ± 0.03	1.475MetPro: 1.475 ± 0.039	1.094MetGln: 1.094 ± 0.029	1.999MetArg: 1.999 ± 0.045	1.711MetSer: 1.711 ± 0.043	1.54MetThr: 1.54 ± 0.039	1.717MetVal: 1.717 ± 0.045	0.206MetTrp: 0.206 ± 0.016	0.491MetTyr: 0.491 ± 0.024	0.0MetXaa: 0.0 ± 0.0
Asn	3.143AsnAla: 3.143 ± 0.067	0.243AsnCys: 0.243 ± 0.015	1.19AsnAsp: 1.19 ± 0.04	1.173AsnGlu: 1.173 ± 0.036	0.865AsnPhe: 0.865 ± 0.03	2.218AsnGly: 2.218 ± 0.058	0.487AsnHis: 0.487 ± 0.022	1.255AsnIle: 1.255 ± 0.038	0.818AsnLys: 0.818 ± 0.032	2.742AsnLeu: 2.742 ± 0.057	0.606AsnMet: 0.606 ± 0.029	0.729AsnAsn: 0.729 ± 0.032	1.93AsnPro: 1.93 ± 0.045	0.834AsnGln: 0.834 ± 0.031	1.765AsnArg: 1.765 ± 0.049	1.129AsnSer: 1.129 ± 0.036	1.422AsnThr: 1.422 ± 0.037	2.025AsnVal: 2.025 ± 0.049	0.375AsnTrp: 0.375 ± 0.019	0.729AsnTyr: 0.729 ± 0.03	0.0AsnXaa: 0.0 ± 0.0
Pro	7.267ProAla: 7.267 ± 0.102	0.393ProCys: 0.393 ± 0.023	3.518ProAsp: 3.518 ± 0.059	3.467ProGlu: 3.467 ± 0.072	1.844ProPhe: 1.844 ± 0.046	5.126ProGly: 5.126 ± 0.082	1.125ProHis: 1.125 ± 0.034	1.914ProIle: 1.914 ± 0.035	1.298ProLys: 1.298 ± 0.042	5.143ProLeu: 5.143 ± 0.081	1.374ProMet: 1.374 ± 0.04	1.251ProAsn: 1.251 ± 0.042	2.769ProPro: 2.769 ± 0.063	2.12ProGln: 2.12 ± 0.049	3.566ProArg: 3.566 ± 0.067	2.678ProSer: 2.678 ± 0.052	2.236ProThr: 2.236 ± 0.049	4.397ProVal: 4.397 ± 0.07	0.808ProTrp: 0.808 ± 0.027	1.531ProTyr: 1.531 ± 0.044	0.002ProXaa: 0.002 ± 0.002
Gln	5.675GlnAla: 5.675 ± 0.085	0.281GlnCys: 0.281 ± 0.019	1.663GlnAsp: 1.663 ± 0.042	1.823GlnGlu: 1.823 ± 0.045	1.194GlnPhe: 1.194 ± 0.034	3.121GlnGly: 3.121 ± 0.06	0.865GlnHis: 0.865 ± 0.03	1.762GlnIle: 1.762 ± 0.045	1.066GlnLys: 1.066 ± 0.033	3.432GlnLeu: 3.432 ± 0.071	0.998GlnMet: 0.998 ± 0.035	0.876GlnAsn: 0.876 ± 0.032	2.131GlnPro: 2.131 ± 0.054	1.638GlnGln: 1.638 ± 0.049	3.034GlnArg: 3.034 ± 0.064	1.813GlnSer: 1.813 ± 0.041	1.768GlnThr: 1.768 ± 0.042	2.826GlnVal: 2.826 ± 0.056	0.61GlnTrp: 0.61 ± 0.02	0.948GlnTyr: 0.948 ± 0.033	0.001GlnXaa: 0.001 ± 0.001
Arg	8.826ArgAla: 8.826 ± 0.107	0.637ArgCys: 0.637 ± 0.029	3.81ArgAsp: 3.81 ± 0.068	4.668ArgGlu: 4.668 ± 0.075	2.674ArgPhe: 2.674 ± 0.051	5.154ArgGly: 5.154 ± 0.078	2.038ArgHis: 2.038 ± 0.051	3.994ArgIle: 3.994 ± 0.069	2.353ArgLys: 2.353 ± 0.059	8.258ArgLeu: 8.258 ± 0.107	2.092ArgMet: 2.092 ± 0.046	2.053ArgAsn: 2.053 ± 0.051	3.733ArgPro: 3.733 ± 0.069	3.238ArgGln: 3.238 ± 0.069	6.174ArgArg: 6.174 ± 0.091	3.44ArgSer: 3.44 ± 0.062	3.106ArgThr: 3.106 ± 0.057	5.408ArgVal: 5.408 ± 0.08	1.119ArgTrp: 1.119 ± 0.04	2.312ArgTyr: 2.312 ± 0.052	0.001ArgXaa: 0.001 ± 0.001
Ser	6.102SerAla: 6.102 ± 0.08	0.443SerCys: 0.443 ± 0.02	2.518SerAsp: 2.518 ± 0.056	2.435SerGlu: 2.435 ± 0.049	2.064SerPhe: 2.064 ± 0.048	5.129SerGly: 5.129 ± 0.081	1.149SerHis: 1.149 ± 0.037	2.32SerIle: 2.32 ± 0.048	1.461SerLys: 1.461 ± 0.045	5.659SerLeu: 5.659 ± 0.087	1.411SerMet: 1.411 ± 0.039	1.288SerAsn: 1.288 ± 0.042	2.908SerPro: 2.908 ± 0.054	1.803SerGln: 1.803 ± 0.048	3.859SerArg: 3.859 ± 0.067	2.887SerSer: 2.887 ± 0.057	2.613SerThr: 2.613 ± 0.049	4.013SerVal: 4.013 ± 0.067	0.788SerTrp: 0.788 ± 0.031	1.335SerTyr: 1.335 ± 0.041	0.001SerXaa: 0.001 ± 0.001
Thr	5.414ThrAla: 5.414 ± 0.066	0.372ThrCys: 0.372 ± 0.022	2.458ThrAsp: 2.458 ± 0.056	2.298ThrGlu: 2.298 ± 0.051	1.609ThrPhe: 1.609 ± 0.043	4.432ThrGly: 4.432 ± 0.068	1.092ThrHis: 1.092 ± 0.035	2.04ThrIle: 2.04 ± 0.05	0.949ThrLys: 0.949 ± 0.036	6.238ThrLeu: 6.238 ± 0.08	1.045ThrMet: 1.045 ± 0.038	1.08ThrAsn: 1.08 ± 0.039	3.319ThrPro: 3.319 ± 0.059	1.674ThrGln: 1.674 ± 0.041	3.413ThrArg: 3.413 ± 0.053	2.476ThrSer: 2.476 ± 0.047	2.233ThrThr: 2.233 ± 0.057	4.021ThrVal: 4.021 ± 0.065	0.625ThrTrp: 0.625 ± 0.027	1.15ThrTyr: 1.15 ± 0.04	0.0ThrXaa: 0.0 ± 0.0
Val	9.396ValAla: 9.396 ± 0.11	0.792ValCys: 0.792 ± 0.027	3.952ValAsp: 3.952 ± 0.069	4.159ValGlu: 4.159 ± 0.07	3.034ValPhe: 3.034 ± 0.06	5.461ValGly: 5.461 ± 0.079	1.465ValHis: 1.465 ± 0.038	3.472ValIle: 3.472 ± 0.066	2.31ValLys: 2.31 ± 0.056	8.272ValLeu: 8.272 ± 0.117	1.9ValMet: 1.9 ± 0.05	2.088ValAsn: 2.088 ± 0.053	4.073ValPro: 4.073 ± 0.067	2.657ValGln: 2.657 ± 0.048	5.537ValArg: 5.537 ± 0.08	4.461ValSer: 4.461 ± 0.066	3.801ValThr: 3.801 ± 0.07	6.296ValVal: 6.296 ± 0.092	0.913ValTrp: 0.913 ± 0.034	1.823ValTyr: 1.823 ± 0.051	0.0ValXaa: 0.0 ± 0.0
Trp	1.255TrpAla: 1.255 ± 0.038	0.137TrpCys: 0.137 ± 0.013	0.598TrpAsp: 0.598 ± 0.027	0.591TrpGlu: 0.591 ± 0.027	0.534TrpPhe: 0.534 ± 0.023	0.962TrpGly: 0.962 ± 0.034	0.341TrpHis: 0.341 ± 0.022	0.649TrpIle: 0.649 ± 0.028	0.515TrpLys: 0.515 ± 0.022	1.982TrpLeu: 1.982 ± 0.05	0.425TrpMet: 0.425 ± 0.019	0.443TrpAsn: 0.443 ± 0.022	0.713TrpPro: 0.713 ± 0.031	0.638TrpGln: 0.638 ± 0.027	1.291TrpArg: 1.291 ± 0.043	0.693TrpSer: 0.693 ± 0.026	0.696TrpThr: 0.696 ± 0.027	0.926TrpVal: 0.926 ± 0.035	0.212TrpTrp: 0.212 ± 0.015	0.336TrpTyr: 0.336 ± 0.02	0.001TrpXaa: 0.001 ± 0.001
Tyr	3.161TyrAla: 3.161 ± 0.067	0.285TyrCys: 0.285 ± 0.018	1.556TyrAsp: 1.556 ± 0.038	1.332TyrGlu: 1.332 ± 0.04	0.946TyrPhe: 0.946 ± 0.032	2.391TyrGly: 2.391 ± 0.055	0.439TyrHis: 0.439 ± 0.022	0.893TyrIle: 0.893 ± 0.031	0.743TyrLys: 0.743 ± 0.036	2.683TyrLeu: 2.683 ± 0.056	0.489TyrMet: 0.489 ± 0.022	0.624TyrAsn: 0.624 ± 0.026	1.411TyrPro: 1.411 ± 0.04	0.911TyrGln: 0.911 ± 0.031	2.072TyrArg: 2.072 ± 0.056	1.273TyrSer: 1.273 ± 0.04	1.391TyrThr: 1.391 ± 0.037	1.939TyrVal: 1.939 ± 0.053	0.385TyrTrp: 0.385 ± 0.02	0.656TyrTyr: 0.656 ± 0.027	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.004XaaAla: 0.004 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.001	0.001XaaLeu: 0.001 ± 0.001	0.001XaaMet: 0.001 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.002XaaSer: 0.002 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.004XaaXaa: 0.004 ± 0.003

Statistics based on 3366 proteins (969022 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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