Pseudocercospora eumusae

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Dothideomycetidae; Mycosphaerellales; Mycosphaerellaceae; Pseudocercospora

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11956 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A139HHU4|A0A139HHU4_9PEZI Uncharacterized protein OS=Pseudocercospora eumusae OX=321146 GN=AC578_6514 PE=4 SV=1
MM1 pKa = 8.1DD2 pKa = 3.6EE3 pKa = 4.72TYY5 pKa = 11.24SHH7 pKa = 7.46DD8 pKa = 3.98LVALAVKK15 pKa = 9.43MYY17 pKa = 9.75NSKK20 pKa = 10.62IIFAVLAAAIAVVRR34 pKa = 11.84SEE36 pKa = 4.44DD37 pKa = 4.11LEE39 pKa = 4.72PNDD42 pKa = 3.98VPPEE46 pKa = 4.12CRR48 pKa = 11.84SICQPAIDD56 pKa = 5.25LEE58 pKa = 4.35NSCDD62 pKa = 3.71RR63 pKa = 11.84QFDD66 pKa = 5.06DD67 pKa = 6.16DD68 pKa = 6.45DD69 pKa = 4.89NDD71 pKa = 4.03NNNSDD76 pKa = 3.28QQYY79 pKa = 10.35RR80 pKa = 11.84DD81 pKa = 4.26CVCKK85 pKa = 10.54AQNANQALSACQNCVVQYY103 pKa = 10.1PNYY106 pKa = 10.52FIDD109 pKa = 5.58ADD111 pKa = 5.3DD112 pKa = 5.46NDD114 pKa = 4.21TDD116 pKa = 5.21DD117 pKa = 5.14NDD119 pKa = 3.57IEE121 pKa = 4.24EE122 pKa = 4.35WLRR125 pKa = 11.84DD126 pKa = 3.9CGFSTATNQTGTAGVTPSPTRR147 pKa = 11.84GPGATGIFSGSLTTATYY164 pKa = 9.05TYY166 pKa = 10.85TEE168 pKa = 4.72IDD170 pKa = 4.71DD171 pKa = 6.02DD172 pKa = 6.51DD173 pKa = 7.46DD174 pKa = 6.33DD175 pKa = 5.01DD176 pKa = 4.71QPEE179 pKa = 4.48TEE181 pKa = 4.06TRR183 pKa = 11.84TTVFPVGAWTSGTFTGSYY201 pKa = 10.93GSLTTSTATYY211 pKa = 7.9TVPDD215 pKa = 5.37DD216 pKa = 6.44DD217 pKa = 7.33DD218 pKa = 7.26DD219 pKa = 7.56DD220 pKa = 6.46DD221 pKa = 6.92DD222 pKa = 5.56NDD224 pKa = 4.1SDD226 pKa = 4.39TITRR230 pKa = 11.84TLVFPAGATPSVPGQNTVSPTTGSVSFTTGTTTDD264 pKa = 3.18AQGNTEE270 pKa = 3.84VRR272 pKa = 11.84LRR274 pKa = 11.84LQDD277 pKa = 3.87LFLVPVWLSCWRR289 pKa = 11.84AWLYY293 pKa = 10.88RR294 pKa = 11.84GIVCTSKK301 pKa = 10.82RR302 pKa = 11.84LSCGLGLDD310 pKa = 3.36ICSGRR315 pKa = 11.84GRR317 pKa = 11.84LL318 pKa = 3.59

Molecular weight: 34.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A139GWA0|A0A139GWA0_9PEZI Isoform of A0A139GW97 60S ribosomal protein L36 OS=Pseudocercospora eumusae OX=321146 GN=AC578_1714 PE=3 SV=1
MM1 pKa = 7.25GQPSHH6 pKa = 6.97PASNALIYY14 pKa = 10.39CSYY17 pKa = 11.37AAFLVFGLFIAWRR30 pKa = 11.84LRR32 pKa = 11.84GQCAGEE38 pKa = 3.79WLSANRR44 pKa = 11.84TQKK47 pKa = 10.93AFPLALNFIASGEE60 pKa = 4.21FGLDD64 pKa = 2.89APRR67 pKa = 11.84IIVSSGRR74 pKa = 11.84RR75 pKa = 11.84QAGWKK80 pKa = 10.13CPEE83 pKa = 4.22TTSSNTKK90 pKa = 9.71VRR92 pKa = 11.84THH94 pKa = 7.02AFRR97 pKa = 11.84DD98 pKa = 3.43IALSAMILGSFARR111 pKa = 11.84RR112 pKa = 11.84VMCSTAGAKK121 pKa = 10.48GNILQVLCSRR131 pKa = 11.84YY132 pKa = 8.52LTDD135 pKa = 3.77CCTKK139 pKa = 10.41LRR141 pKa = 11.84RR142 pKa = 11.84TAMTRR147 pKa = 11.84SRR149 pKa = 3.84

Molecular weight: 16.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10939

1017

11956

5580759

34

8267

466.8

51.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.072 ± 0.02

1.265 ± 0.008

5.749 ± 0.015

6.196 ± 0.023

3.584 ± 0.013

6.722 ± 0.022

2.556 ± 0.01

4.725 ± 0.015

5.005 ± 0.018

8.584 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.233 ± 0.008

3.699 ± 0.013

6.0 ± 0.025

4.304 ± 0.016

6.188 ± 0.02

8.109 ± 0.026

5.971 ± 0.016

5.802 ± 0.017

1.47 ± 0.007

2.764 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski