Culex tritaeniorhynchus rhabdovirus
Average proteome isoelectric point is 7.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F7J0V0|F7J0V0_9RHAB Phosphoprotein OS=Culex tritaeniorhynchus rhabdovirus OX=936308 GN=P PE=4 SV=2
MM1 pKa = 7.74 SDD3 pKa = 3.51 PVCRR7 pKa = 11.84 HH8 pKa = 5.83 GNLEE12 pKa = 4.44 GYY14 pKa = 8.38 QVPSISLASTIKK26 pKa = 9.7 ATYY29 pKa = 9.19 PSEE32 pKa = 4.69 AFAKK36 pKa = 9.35 QEE38 pKa = 3.93 KK39 pKa = 9.89 PIVRR43 pKa = 11.84 GPAYY47 pKa = 9.64 TGDD50 pKa = 3.59 LAGFDD55 pKa = 4.75 AYY57 pKa = 8.48 MTSQLSQTTVTSLPIDD73 pKa = 3.52 YY74 pKa = 10.34 AICYY78 pKa = 8.02 LHH80 pKa = 7.16 AVACVTGVKK89 pKa = 10.37 LNEE92 pKa = 3.96 PWSSFGVNLTDD103 pKa = 4.6 AEE105 pKa = 4.43 NNVYY109 pKa = 9.98 IASLYY114 pKa = 10.27 KK115 pKa = 10.1 FQAVGDD121 pKa = 4.14 ALDD124 pKa = 3.91 LSGGAVQGDD133 pKa = 3.57 RR134 pKa = 11.84 LYY136 pKa = 11.66 NLFMLFAPCRR146 pKa = 11.84 LHH148 pKa = 7.17 SGLRR152 pKa = 11.84 PEE154 pKa = 4.26 YY155 pKa = 10.21 RR156 pKa = 11.84 PLLAEE161 pKa = 4.3 RR162 pKa = 11.84 YY163 pKa = 6.1 KK164 pKa = 10.89 TVMGDD169 pKa = 3.12 KK170 pKa = 10.61 KK171 pKa = 10.88 KK172 pKa = 10.75 GLEE175 pKa = 3.98 PSALAIKK182 pKa = 10.13 CAGWDD187 pKa = 3.3 QHH189 pKa = 5.95 PAYY192 pKa = 9.99 AALAAAYY199 pKa = 10.76 DD200 pKa = 3.54 MFLFKK205 pKa = 10.66 TEE207 pKa = 3.53 NHH209 pKa = 6.58 EE210 pKa = 4.13 YY211 pKa = 10.46 AKK213 pKa = 10.64 VRR215 pKa = 11.84 IGTTPMRR222 pKa = 11.84 FRR224 pKa = 11.84 DD225 pKa = 3.89 CTMLGVLEE233 pKa = 4.22 VMRR236 pKa = 11.84 NAMKK240 pKa = 10.51 VEE242 pKa = 4.05 SMIDD246 pKa = 3.06 ATYY249 pKa = 9.56 WIWNGRR255 pKa = 11.84 LADD258 pKa = 4.14 EE259 pKa = 4.55 FEE261 pKa = 4.85 KK262 pKa = 10.24 IYY264 pKa = 11.05 KK265 pKa = 10.05 EE266 pKa = 4.52 GEE268 pKa = 4.11 EE269 pKa = 4.02 LDD271 pKa = 4.12 KK272 pKa = 11.41 ADD274 pKa = 5.07 SYY276 pKa = 10.18 TPYY279 pKa = 10.76 YY280 pKa = 10.97 AGFKK284 pKa = 10.09 LGNRR288 pKa = 11.84 SPYY291 pKa = 9.36 SATMSPQLHH300 pKa = 5.92 YY301 pKa = 10.15 FVHH304 pKa = 6.99 TIGCLANSKK313 pKa = 10.42 RR314 pKa = 11.84 SINARR319 pKa = 11.84 AITEE323 pKa = 4.01 VGIEE327 pKa = 4.08 DD328 pKa = 3.91 ARR330 pKa = 11.84 DD331 pKa = 3.03 NAIIVGYY338 pKa = 10.51 VMAGRR343 pKa = 11.84 RR344 pKa = 11.84 RR345 pKa = 11.84 HH346 pKa = 5.19 RR347 pKa = 11.84 AQYY350 pKa = 9.35 FRR352 pKa = 11.84 PSEE355 pKa = 4.26 AEE357 pKa = 3.62 LWKK360 pKa = 10.83 APEE363 pKa = 3.92 PRR365 pKa = 11.84 PRR367 pKa = 11.84 ADD369 pKa = 4.03 CLPGANEE376 pKa = 3.93 AVRR379 pKa = 11.84 DD380 pKa = 3.82 RR381 pKa = 11.84 GQEE384 pKa = 3.91 DD385 pKa = 4.13 EE386 pKa = 4.44 EE387 pKa = 5.49 SSVSEE392 pKa = 4.1 VSSVCSRR399 pKa = 11.84 IARR402 pKa = 11.84 DD403 pKa = 3.44 FDD405 pKa = 3.86 GEE407 pKa = 4.47 EE408 pKa = 3.81 IPEE411 pKa = 4.37 PLSSRR416 pKa = 11.84 ASDD419 pKa = 3.64 WYY421 pKa = 10.89 QYY423 pKa = 9.03 MASQHH428 pKa = 6.43 FIMPRR433 pKa = 11.84 QMEE436 pKa = 3.99 IAACNILAALDD447 pKa = 3.49 KK448 pKa = 10.49 TRR450 pKa = 11.84 PDD452 pKa = 3.73 TIGDD456 pKa = 4.06 HH457 pKa = 6.35 LKK459 pKa = 8.79 MWAKK463 pKa = 10.67 AKK465 pKa = 10.49 LRR467 pKa = 11.84 TLQQTT472 pKa = 3.54
Molecular weight: 52.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.829
IPC2_protein 5.906
IPC_protein 5.97
Toseland 6.237
ProMoST 6.313
Dawson 6.249
Bjellqvist 6.249
Wikipedia 6.249
Rodwell 6.237
Grimsley 6.351
Solomon 6.249
Lehninger 6.237
Nozaki 6.517
DTASelect 6.693
Thurlkill 6.707
EMBOSS 6.664
Sillero 6.62
Patrickios 3.91
IPC_peptide 6.262
IPC2_peptide 6.561
IPC2.peptide.svr19 6.545
Protein with the highest isoelectric point:
>tr|F7J0V2|F7J0V2_9RHAB Glycoprotein OS=Culex tritaeniorhynchus rhabdovirus OX=936308 GN=G PE=4 SV=1
MM1 pKa = 7.1 STSQVGVKK9 pKa = 9.66 SFKK12 pKa = 10.51 ILFDD16 pKa = 3.96 LQLSSDD22 pKa = 3.48 TRR24 pKa = 11.84 FNNCLDD30 pKa = 4.17 LACGISEE37 pKa = 4.18 VRR39 pKa = 11.84 DD40 pKa = 3.62 AMRR43 pKa = 11.84 GTPTEE48 pKa = 3.89 VSFLLGVLGLGLYY61 pKa = 9.89 GSKK64 pKa = 10.66 AEE66 pKa = 4.26 KK67 pKa = 10.15 LAAGGSWSKK76 pKa = 10.53 RR77 pKa = 11.84 VEE79 pKa = 4.76 DD80 pKa = 3.49 IVKK83 pKa = 9.46 TKK85 pKa = 9.85 TAVALLKK92 pKa = 10.93 SPIARR97 pKa = 11.84 SFRR100 pKa = 11.84 YY101 pKa = 9.7 EE102 pKa = 3.44 NSLTLRR108 pKa = 11.84 GARR111 pKa = 11.84 TYY113 pKa = 11.05 IRR115 pKa = 11.84 MSVQISEE122 pKa = 4.31 TTLDD126 pKa = 3.1 GRR128 pKa = 11.84 RR129 pKa = 11.84 YY130 pKa = 9.86 RR131 pKa = 11.84 EE132 pKa = 3.87 VFGLMDD138 pKa = 4.35 KK139 pKa = 11.12 GKK141 pKa = 7.72 PTPKK145 pKa = 10.64 GPIKK149 pKa = 10.35 EE150 pKa = 4.15 VLAVFGVTLEE160 pKa = 4.37 SVGGLDD166 pKa = 3.98 EE167 pKa = 5.95 LSMISLL173 pKa = 4.34
Molecular weight: 18.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.003
IPC2_protein 8.741
IPC_protein 8.712
Toseland 9.531
ProMoST 9.209
Dawson 9.75
Bjellqvist 9.443
Wikipedia 9.882
Rodwell 10.116
Grimsley 9.794
Solomon 9.809
Lehninger 9.794
Nozaki 9.633
DTASelect 9.399
Thurlkill 9.604
EMBOSS 9.94
Sillero 9.692
Patrickios 7.644
IPC_peptide 9.809
IPC2_peptide 8.039
IPC2.peptide.svr19 7.871
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3535
173
2123
707.0
78.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.496 ± 0.995
1.754 ± 0.221
5.516 ± 0.612
5.941 ± 0.21
3.876 ± 0.534
7.666 ± 0.843
2.405 ± 0.367
5.969 ± 0.554
4.668 ± 0.432
9.901 ± 0.625
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.207 ± 0.28
3.338 ± 0.512
5.488 ± 0.691
2.744 ± 0.311
6.478 ± 0.201
7.61 ± 1.104
5.827 ± 0.301
5.912 ± 0.402
2.008 ± 0.269
3.197 ± 0.479
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here