Bacillus sp. OK048

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5056 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H0BN61|A0A1H0BN61_9BACI Acetoacetyl-CoA synthetase OS=Bacillus sp. OK048 OX=1882761 GN=SAMN05443253_11293 PE=3 SV=1
MM1 pKa = 7.5SLEE4 pKa = 3.92WFDD7 pKa = 4.15RR8 pKa = 11.84VCSEE12 pKa = 4.49LQDD15 pKa = 3.73HH16 pKa = 6.83LEE18 pKa = 4.58SICEE22 pKa = 4.06EE23 pKa = 4.28YY24 pKa = 11.0NQVGQMSIEE33 pKa = 4.15RR34 pKa = 11.84ASKK37 pKa = 9.74HH38 pKa = 4.75PRR40 pKa = 11.84IEE42 pKa = 4.2FFVEE46 pKa = 4.12TEE48 pKa = 4.03DD49 pKa = 4.56VQTEE53 pKa = 4.42DD54 pKa = 2.75IDD56 pKa = 4.45RR57 pKa = 11.84DD58 pKa = 4.05YY59 pKa = 11.54FCTLFFDD66 pKa = 4.45PGNEE70 pKa = 3.63EE71 pKa = 4.51FYY73 pKa = 10.66IDD75 pKa = 4.07TFDD78 pKa = 3.5VDD80 pKa = 3.74SGHH83 pKa = 5.37TAKK86 pKa = 10.56IILSDD91 pKa = 3.23IEE93 pKa = 4.26EE94 pKa = 4.91IIDD97 pKa = 3.59EE98 pKa = 4.34VHH100 pKa = 6.94ISLHH104 pKa = 7.34DD105 pKa = 3.71YY106 pKa = 10.9MNDD109 pKa = 3.29DD110 pKa = 4.43DD111 pKa = 6.19HH112 pKa = 6.46YY113 pKa = 9.45TDD115 pKa = 4.69DD116 pKa = 4.79GVYY119 pKa = 8.61LTSNEE124 pKa = 4.09VYY126 pKa = 10.2EE127 pKa = 5.73DD128 pKa = 3.8SDD130 pKa = 3.47DD131 pKa = 4.1CYY133 pKa = 11.82EE134 pKa = 4.07MVEE137 pKa = 4.02VDD139 pKa = 4.32DD140 pKa = 6.33DD141 pKa = 4.12GVEE144 pKa = 4.31YY145 pKa = 10.87YY146 pKa = 10.15QAEE149 pKa = 4.39QVNSDD154 pKa = 4.77DD155 pKa = 3.48IFEE158 pKa = 5.14EE159 pKa = 5.19IDD161 pKa = 3.58VDD163 pKa = 3.77WNTPEE168 pKa = 3.74VTAFKK173 pKa = 10.4HH174 pKa = 5.13GNEE177 pKa = 4.16VEE179 pKa = 4.1VTYY182 pKa = 10.92QFGVVSEE189 pKa = 4.42TGDD192 pKa = 3.46GVLKK196 pKa = 10.46RR197 pKa = 11.84INRR200 pKa = 11.84IWTTNDD206 pKa = 2.63EE207 pKa = 4.75LIRR210 pKa = 11.84DD211 pKa = 3.73EE212 pKa = 4.36SHH214 pKa = 7.34FIFSKK219 pKa = 10.93EE220 pKa = 3.83EE221 pKa = 3.63ASTIIAMIASHH232 pKa = 6.76MDD234 pKa = 3.12QLSEE238 pKa = 4.74FNFDD242 pKa = 5.1DD243 pKa = 3.93MPP245 pKa = 6.42

Molecular weight: 28.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H0CZM9|A0A1H0CZM9_9BACI EthD domain-containing protein OS=Bacillus sp. OK048 OX=1882761 GN=SAMN05443253_11558 PE=4 SV=1
MM1 pKa = 7.79PFYY4 pKa = 10.32PPNPGRR10 pKa = 11.84RR11 pKa = 11.84PFPPGNRR18 pKa = 11.84QRR20 pKa = 11.84FGPSNQMGRR29 pKa = 11.84RR30 pKa = 11.84PRR32 pKa = 11.84PFNVPNQNQQAPTSRR47 pKa = 11.84FQGLKK52 pKa = 10.98AMMGHH57 pKa = 6.48VGTVRR62 pKa = 11.84SGINTLRR69 pKa = 11.84QVGSFLKK76 pKa = 10.53FFKK79 pKa = 10.82

Molecular weight: 8.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5056

0

5056

1448404

26

1961

286.5

32.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.731 ± 0.04

0.748 ± 0.011

4.961 ± 0.028

7.305 ± 0.048

4.718 ± 0.032

6.938 ± 0.042

2.038 ± 0.017

8.105 ± 0.039

7.12 ± 0.033

9.78 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.73 ± 0.017

4.724 ± 0.028

3.621 ± 0.021

3.604 ± 0.024

3.923 ± 0.028

6.021 ± 0.023

5.428 ± 0.027

6.942 ± 0.028

1.064 ± 0.014

3.502 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski