Amino acid dipepetide frequency for Bacillus sp. OK048

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.789AlaAla: 5.789 ± 0.089
0.57AlaCys: 0.57 ± 0.022
3.254AlaAsp: 3.254 ± 0.06
4.415AlaGlu: 4.415 ± 0.058
3.133AlaPhe: 3.133 ± 0.043
5.262AlaGly: 5.262 ± 0.072
1.214AlaHis: 1.214 ± 0.031
5.836AlaIle: 5.836 ± 0.065
4.668AlaLys: 4.668 ± 0.059
6.685AlaLeu: 6.685 ± 0.07
1.923AlaMet: 1.923 ± 0.039
2.88AlaAsn: 2.88 ± 0.046
2.028AlaPro: 2.028 ± 0.044
2.075AlaGln: 2.075 ± 0.043
2.477AlaArg: 2.477 ± 0.039
3.745AlaSer: 3.745 ± 0.052
3.368AlaThr: 3.368 ± 0.061
5.146AlaVal: 5.146 ± 0.078
0.617AlaTrp: 0.617 ± 0.021
2.22AlaTyr: 2.22 ± 0.037
0.0AlaXaa: 0.0 ± 0.0
Cys
0.444CysAla: 0.444 ± 0.019
0.101CysCys: 0.101 ± 0.009
0.372CysAsp: 0.372 ± 0.016
0.511CysGlu: 0.511 ± 0.021
0.345CysPhe: 0.345 ± 0.018
0.704CysGly: 0.704 ± 0.025
0.239CysHis: 0.239 ± 0.017
0.568CysIle: 0.568 ± 0.022
0.38CysLys: 0.38 ± 0.017
0.698CysLeu: 0.698 ± 0.024
0.185CysMet: 0.185 ± 0.01
0.32CysAsn: 0.32 ± 0.015
0.362CysPro: 0.362 ± 0.019
0.234CysGln: 0.234 ± 0.013
0.293CysArg: 0.293 ± 0.015
0.53CysSer: 0.53 ± 0.019
0.412CysThr: 0.412 ± 0.017
0.442CysVal: 0.442 ± 0.018
0.068CysTrp: 0.068 ± 0.008
0.268CysTyr: 0.268 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
2.891AspAla: 2.891 ± 0.044
0.392AspCys: 0.392 ± 0.017
2.273AspAsp: 2.273 ± 0.042
4.056AspGlu: 4.056 ± 0.055
2.556AspPhe: 2.556 ± 0.04
3.338AspGly: 3.338 ± 0.055
1.17AspHis: 1.17 ± 0.027
4.006AspIle: 4.006 ± 0.06
3.197AspLys: 3.197 ± 0.05
5.018AspLeu: 5.018 ± 0.059
1.179AspMet: 1.179 ± 0.029
1.922AspAsn: 1.922 ± 0.042
1.992AspPro: 1.992 ± 0.04
1.908AspGln: 1.908 ± 0.034
2.182AspArg: 2.182 ± 0.042
2.727AspSer: 2.727 ± 0.049
2.374AspThr: 2.374 ± 0.051
3.667AspVal: 3.667 ± 0.052
0.669AspTrp: 0.669 ± 0.022
2.092AspTyr: 2.092 ± 0.041
0.0AspXaa: 0.0 ± 0.0
Glu
4.985GluAla: 4.985 ± 0.073
0.416GluCys: 0.416 ± 0.018
3.473GluAsp: 3.473 ± 0.048
6.373GluGlu: 6.373 ± 0.077
2.822GluPhe: 2.822 ± 0.041
4.185GluGly: 4.185 ± 0.058
1.395GluHis: 1.395 ± 0.031
5.84GluIle: 5.84 ± 0.067
6.765GluLys: 6.765 ± 0.074
7.15GluLeu: 7.15 ± 0.071
2.218GluMet: 2.218 ± 0.038
3.912GluAsn: 3.912 ± 0.061
1.884GluPro: 1.884 ± 0.04
2.996GluGln: 2.996 ± 0.05
3.209GluArg: 3.209 ± 0.047
3.471GluSer: 3.471 ± 0.044
3.745GluThr: 3.745 ± 0.052
4.956GluVal: 4.956 ± 0.061
0.855GluTrp: 0.855 ± 0.024
2.394GluTyr: 2.394 ± 0.037
0.0GluXaa: 0.0 ± 0.0
Phe
3.016PheAla: 3.016 ± 0.049
0.389PheCys: 0.389 ± 0.018
2.418PheAsp: 2.418 ± 0.044
3.029PheGlu: 3.029 ± 0.05
2.454PhePhe: 2.454 ± 0.054
3.444PheGly: 3.444 ± 0.045
1.036PheHis: 1.036 ± 0.026
4.153PheIle: 4.153 ± 0.065
2.642PheLys: 2.642 ± 0.044
4.829PheLeu: 4.829 ± 0.062
1.169PheMet: 1.169 ± 0.033
2.104PheAsn: 2.104 ± 0.039
1.716PhePro: 1.716 ± 0.033
1.606PheGln: 1.606 ± 0.038
1.598PheArg: 1.598 ± 0.031
3.365PheSer: 3.365 ± 0.048
2.733PheThr: 2.733 ± 0.039
3.169PheVal: 3.169 ± 0.054
0.512PheTrp: 0.512 ± 0.021
1.796PheTyr: 1.796 ± 0.036
0.0PheXaa: 0.0 ± 0.0
Gly
4.708GlyAla: 4.708 ± 0.061
0.65GlyCys: 0.65 ± 0.026
3.132GlyAsp: 3.132 ± 0.046
4.376GlyGlu: 4.376 ± 0.056
3.548GlyPhe: 3.548 ± 0.061
4.941GlyGly: 4.941 ± 0.08
1.398GlyHis: 1.398 ± 0.034
6.216GlyIle: 6.216 ± 0.066
5.28GlyLys: 5.28 ± 0.067
6.62GlyLeu: 6.62 ± 0.069
2.115GlyMet: 2.115 ± 0.045
3.01GlyAsn: 3.01 ± 0.06
1.767GlyPro: 1.767 ± 0.043
2.256GlyGln: 2.256 ± 0.045
2.568GlyArg: 2.568 ± 0.042
4.056GlySer: 4.056 ± 0.052
4.072GlyThr: 4.072 ± 0.064
5.007GlyVal: 5.007 ± 0.06
0.788GlyTrp: 0.788 ± 0.026
2.869GlyTyr: 2.869 ± 0.045
0.0GlyXaa: 0.0 ± 0.0
His
1.188HisAla: 1.188 ± 0.032
0.213HisCys: 0.213 ± 0.012
1.072HisAsp: 1.072 ± 0.029
1.339HisGlu: 1.339 ± 0.03
1.059HisPhe: 1.059 ± 0.024
1.438HisGly: 1.438 ± 0.032
0.632HisHis: 0.632 ± 0.024
1.425HisIle: 1.425 ± 0.027
0.965HisLys: 0.965 ± 0.026
2.084HisLeu: 2.084 ± 0.041
0.467HisMet: 0.467 ± 0.018
0.82HisAsn: 0.82 ± 0.025
1.099HisPro: 1.099 ± 0.028
0.843HisGln: 0.843 ± 0.024
0.875HisArg: 0.875 ± 0.025
1.292HisSer: 1.292 ± 0.03
1.05HisThr: 1.05 ± 0.027
1.342HisVal: 1.342 ± 0.029
0.226HisTrp: 0.226 ± 0.014
0.951HisTyr: 0.951 ± 0.023
0.0HisXaa: 0.0 ± 0.0
Ile
5.868IleAla: 5.868 ± 0.06
0.665IleCys: 0.665 ± 0.021
4.333IleAsp: 4.333 ± 0.053
5.941IleGlu: 5.941 ± 0.069
3.587IlePhe: 3.587 ± 0.063
6.07IleGly: 6.07 ± 0.072
1.7IleHis: 1.7 ± 0.035
6.31IleIle: 6.31 ± 0.085
5.09IleLys: 5.09 ± 0.064
7.521IleLeu: 7.521 ± 0.092
1.864IleMet: 1.864 ± 0.037
3.806IleAsn: 3.806 ± 0.051
3.46IlePro: 3.46 ± 0.051
2.906IleGln: 2.906 ± 0.057
3.141IleArg: 3.141 ± 0.05
5.368IleSer: 5.368 ± 0.062
4.624IleThr: 4.624 ± 0.057
5.554IleVal: 5.554 ± 0.068
0.722IleTrp: 0.722 ± 0.023
2.52IleTyr: 2.52 ± 0.048
0.0IleXaa: 0.0 ± 0.0
Lys
4.631LysAla: 4.631 ± 0.069
0.376LysCys: 0.376 ± 0.019
3.847LysAsp: 3.847 ± 0.069
6.633LysGlu: 6.633 ± 0.09
2.218LysPhe: 2.218 ± 0.041
4.644LysGly: 4.644 ± 0.059
1.286LysHis: 1.286 ± 0.03
5.265LysIle: 5.265 ± 0.065
6.057LysLys: 6.057 ± 0.078
6.047LysLeu: 6.047 ± 0.073
2.329LysMet: 2.329 ± 0.042
3.821LysAsn: 3.821 ± 0.051
2.329LysPro: 2.329 ± 0.041
2.931LysGln: 2.931 ± 0.04
3.181LysArg: 3.181 ± 0.05
3.775LysSer: 3.775 ± 0.05
3.692LysThr: 3.692 ± 0.056
4.972LysVal: 4.972 ± 0.059
0.9LysTrp: 0.9 ± 0.027
2.265LysTyr: 2.265 ± 0.041
0.0LysXaa: 0.0 ± 0.0
Leu
7.036LeuAla: 7.036 ± 0.073
0.717LeuCys: 0.717 ± 0.023
4.787LeuAsp: 4.787 ± 0.065
6.736LeuGlu: 6.736 ± 0.077
4.919LeuPhe: 4.919 ± 0.072
6.437LeuGly: 6.437 ± 0.074
1.818LeuHis: 1.818 ± 0.032
7.54LeuIle: 7.54 ± 0.087
6.897LeuLys: 6.897 ± 0.076
10.208LeuLeu: 10.208 ± 0.117
2.568LeuMet: 2.568 ± 0.043
4.407LeuAsn: 4.407 ± 0.06
3.876LeuPro: 3.876 ± 0.053
3.359LeuGln: 3.359 ± 0.053
3.51LeuArg: 3.51 ± 0.05
6.711LeuSer: 6.711 ± 0.067
5.83LeuThr: 5.83 ± 0.061
6.491LeuVal: 6.491 ± 0.074
0.842LeuTrp: 0.842 ± 0.024
3.108LeuTyr: 3.108 ± 0.053
0.0LeuXaa: 0.0 ± 0.0
Met
2.037MetAla: 2.037 ± 0.034
0.153MetCys: 0.153 ± 0.01
1.499MetAsp: 1.499 ± 0.036
1.939MetGlu: 1.939 ± 0.036
1.116MetPhe: 1.116 ± 0.03
1.881MetGly: 1.881 ± 0.044
0.402MetHis: 0.402 ± 0.014
2.216MetIle: 2.216 ± 0.044
2.406MetLys: 2.406 ± 0.04
2.455MetLeu: 2.455 ± 0.043
0.818MetMet: 0.818 ± 0.026
1.556MetAsn: 1.556 ± 0.033
0.983MetPro: 0.983 ± 0.029
0.806MetGln: 0.806 ± 0.022
1.009MetArg: 1.009 ± 0.028
1.642MetSer: 1.642 ± 0.035
1.564MetThr: 1.564 ± 0.033
1.886MetVal: 1.886 ± 0.034
0.206MetTrp: 0.206 ± 0.012
0.721MetTyr: 0.721 ± 0.022
0.0MetXaa: 0.0 ± 0.0
Asn
2.76AsnAla: 2.76 ± 0.046
0.347AsnCys: 0.347 ± 0.017
2.352AsnAsp: 2.352 ± 0.04
3.625AsnGlu: 3.625 ± 0.055
1.903AsnPhe: 1.903 ± 0.042
3.605AsnGly: 3.605 ± 0.059
1.169AsnHis: 1.169 ± 0.03
3.475AsnIle: 3.475 ± 0.053
3.297AsnLys: 3.297 ± 0.054
4.367AsnLeu: 4.367 ± 0.05
1.205AsnMet: 1.205 ± 0.03
2.265AsnAsn: 2.265 ± 0.047
2.332AsnPro: 2.332 ± 0.047
2.155AsnGln: 2.155 ± 0.042
2.143AsnArg: 2.143 ± 0.038
2.606AsnSer: 2.606 ± 0.045
2.291AsnThr: 2.291 ± 0.038
3.091AsnVal: 3.091 ± 0.045
0.618AsnTrp: 0.618 ± 0.024
1.638AsnTyr: 1.638 ± 0.036
0.0AsnXaa: 0.0 ± 0.0
Pro
2.344ProAla: 2.344 ± 0.051
0.234ProCys: 0.234 ± 0.014
1.928ProAsp: 1.928 ± 0.039
2.965ProGlu: 2.965 ± 0.046
2.049ProPhe: 2.049 ± 0.041
2.404ProGly: 2.404 ± 0.048
0.736ProHis: 0.736 ± 0.024
3.03ProIle: 3.03 ± 0.046
2.245ProLys: 2.245 ± 0.042
3.486ProLeu: 3.486 ± 0.052
0.869ProMet: 0.869 ± 0.027
1.794ProAsn: 1.794 ± 0.041
1.007ProPro: 1.007 ± 0.027
1.172ProGln: 1.172 ± 0.031
1.126ProArg: 1.126 ± 0.031
2.207ProSer: 2.207 ± 0.042
2.077ProThr: 2.077 ± 0.041
2.771ProVal: 2.771 ± 0.047
0.376ProTrp: 0.376 ± 0.017
1.388ProTyr: 1.388 ± 0.036
0.0ProXaa: 0.0 ± 0.0
Gln
2.412GlnAla: 2.412 ± 0.039
0.226GlnCys: 0.226 ± 0.013
1.567GlnAsp: 1.567 ± 0.031
2.675GlnGlu: 2.675 ± 0.048
1.65GlnPhe: 1.65 ± 0.03
2.167GlnGly: 2.167 ± 0.046
0.744GlnHis: 0.744 ± 0.027
2.689GlnIle: 2.689 ± 0.042
2.711GlnLys: 2.711 ± 0.049
3.919GlnLeu: 3.919 ± 0.058
1.108GlnMet: 1.108 ± 0.031
1.74GlnAsn: 1.74 ± 0.034
1.202GlnPro: 1.202 ± 0.033
1.647GlnGln: 1.647 ± 0.037
1.473GlnArg: 1.473 ± 0.036
2.071GlnSer: 2.071 ± 0.044
1.908GlnThr: 1.908 ± 0.034
2.405GlnVal: 2.405 ± 0.042
0.435GlnTrp: 0.435 ± 0.017
1.288GlnTyr: 1.288 ± 0.032
0.0GlnXaa: 0.0 ± 0.0
Arg
2.332ArgAla: 2.332 ± 0.043
0.25ArgCys: 0.25 ± 0.014
1.959ArgAsp: 1.959 ± 0.039
3.103ArgGlu: 3.103 ± 0.05
1.948ArgPhe: 1.948 ± 0.038
2.309ArgGly: 2.309 ± 0.037
0.792ArgHis: 0.792 ± 0.024
3.199ArgIle: 3.199 ± 0.054
3.138ArgLys: 3.138 ± 0.048
3.848ArgLeu: 3.848 ± 0.063
1.281ArgMet: 1.281 ± 0.031
2.08ArgAsn: 2.08 ± 0.038
1.308ArgPro: 1.308 ± 0.033
1.392ArgGln: 1.392 ± 0.035
1.762ArgArg: 1.762 ± 0.041
2.128ArgSer: 2.128 ± 0.036
1.96ArgThr: 1.96 ± 0.033
2.553ArgVal: 2.553 ± 0.042
0.411ArgTrp: 0.411 ± 0.015
1.481ArgTyr: 1.481 ± 0.034
0.0ArgXaa: 0.0 ± 0.0
Ser
3.629SerAla: 3.629 ± 0.06
0.42SerCys: 0.42 ± 0.018
2.837SerAsp: 2.837 ± 0.04
3.869SerGlu: 3.869 ± 0.05
3.433SerPhe: 3.433 ± 0.054
4.496SerGly: 4.496 ± 0.054
1.216SerHis: 1.216 ± 0.03
5.279SerIle: 5.279 ± 0.061
4.03SerLys: 4.03 ± 0.057
6.144SerLeu: 6.144 ± 0.075
1.705SerMet: 1.705 ± 0.034
2.756SerAsn: 2.756 ± 0.052
2.16SerPro: 2.16 ± 0.038
1.986SerGln: 1.986 ± 0.041
2.286SerArg: 2.286 ± 0.04
3.858SerSer: 3.858 ± 0.054
3.168SerThr: 3.168 ± 0.054
4.062SerVal: 4.062 ± 0.062
0.655SerTrp: 0.655 ± 0.024
2.218SerTyr: 2.218 ± 0.043
0.0SerXaa: 0.0 ± 0.0
Thr
3.857ThrAla: 3.857 ± 0.061
0.356ThrCys: 0.356 ± 0.017
2.58ThrAsp: 2.58 ± 0.05
3.462ThrGlu: 3.462 ± 0.055
2.691ThrPhe: 2.691 ± 0.051
4.265ThrGly: 4.265 ± 0.06
1.085ThrHis: 1.085 ± 0.025
4.852ThrIle: 4.852 ± 0.065
3.536ThrLys: 3.536 ± 0.049
5.213ThrLeu: 5.213 ± 0.054
1.322ThrMet: 1.322 ± 0.03
2.633ThrAsn: 2.633 ± 0.045
2.295ThrPro: 2.295 ± 0.04
1.404ThrGln: 1.404 ± 0.035
1.851ThrArg: 1.851 ± 0.036
3.26ThrSer: 3.26 ± 0.05
2.987ThrThr: 2.987 ± 0.052
4.223ThrVal: 4.223 ± 0.06
0.562ThrTrp: 0.562 ± 0.023
1.844ThrTyr: 1.844 ± 0.042
0.0ThrXaa: 0.0 ± 0.0
Val
4.731ValAla: 4.731 ± 0.064
0.597ValCys: 0.597 ± 0.022
3.514ValAsp: 3.514 ± 0.046
4.921ValGlu: 4.921 ± 0.061
3.262ValPhe: 3.262 ± 0.049
4.598ValGly: 4.598 ± 0.056
1.279ValHis: 1.279 ± 0.03
5.834ValIle: 5.834 ± 0.057
4.977ValLys: 4.977 ± 0.058
6.676ValLeu: 6.676 ± 0.066
1.803ValMet: 1.803 ± 0.033
3.312ValAsn: 3.312 ± 0.051
2.773ValPro: 2.773 ± 0.044
2.33ValGln: 2.33 ± 0.039
2.579ValArg: 2.579 ± 0.04
4.503ValSer: 4.503 ± 0.062
4.118ValThr: 4.118 ± 0.052
4.83ValVal: 4.83 ± 0.066
0.609ValTrp: 0.609 ± 0.024
2.178ValTyr: 2.178 ± 0.037
0.0ValXaa: 0.0 ± 0.0
Trp
0.646TrpAla: 0.646 ± 0.024
0.086TrpCys: 0.086 ± 0.008
0.569TrpAsp: 0.569 ± 0.017
0.705TrpGlu: 0.705 ± 0.022
0.552TrpPhe: 0.552 ± 0.02
0.745TrpGly: 0.745 ± 0.025
0.2TrpHis: 0.2 ± 0.011
0.849TrpIle: 0.849 ± 0.027
0.769TrpLys: 0.769 ± 0.023
1.167TrpLeu: 1.167 ± 0.035
0.334TrpMet: 0.334 ± 0.015
0.601TrpAsn: 0.601 ± 0.021
0.233TrpPro: 0.233 ± 0.013
0.394TrpGln: 0.394 ± 0.016
0.422TrpArg: 0.422 ± 0.02
0.62TrpSer: 0.62 ± 0.021
0.509TrpThr: 0.509 ± 0.019
0.699TrpVal: 0.699 ± 0.023
0.148TrpTrp: 0.148 ± 0.01
0.388TrpTyr: 0.388 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.001TyrAla: 2.001 ± 0.038
0.32TyrCys: 0.32 ± 0.015
1.845TyrAsp: 1.845 ± 0.034
2.376TyrGlu: 2.376 ± 0.038
1.932TyrPhe: 1.932 ± 0.035
2.443TyrGly: 2.443 ± 0.042
0.872TyrHis: 0.872 ± 0.025
2.469TyrIle: 2.469 ± 0.042
2.146TyrLys: 2.146 ± 0.042
3.66TyrLeu: 3.66 ± 0.047
0.833TyrMet: 0.833 ± 0.022
1.504TyrAsn: 1.504 ± 0.031
1.4TyrPro: 1.4 ± 0.032
1.638TyrGln: 1.638 ± 0.038
1.547TyrArg: 1.547 ± 0.038
2.273TyrSer: 2.273 ± 0.045
1.794TyrThr: 1.794 ± 0.036
2.158TyrVal: 2.158 ± 0.035
0.416TyrTrp: 0.416 ± 0.016
1.398TyrTyr: 1.398 ± 0.038
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5056 proteins (1448404 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski