Rotavirus D chicken/05V0049/DEU/2005
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E2EBV0|E2EBV0_9REOV NSP5 OS=Rotavirus D chicken/05V0049/DEU/2005 OX=884200 PE=4 SV=1
MM1 pKa = 7.57 MDD3 pKa = 4.62 DD4 pKa = 4.51 LDD6 pKa = 5.88 FNFEE10 pKa = 4.51 SNLPEE15 pKa = 4.44 ISLISSRR22 pKa = 11.84 AGTTYY27 pKa = 10.73 TKK29 pKa = 9.8 IDD31 pKa = 3.75 YY32 pKa = 10.86 DD33 pKa = 4.1 EE34 pKa = 6.2 DD35 pKa = 3.73 MLLDD39 pKa = 5.8 DD40 pKa = 4.82 ITPSDD45 pKa = 3.88 SASSQDD51 pKa = 3.19 TNQRR55 pKa = 11.84 TFRR58 pKa = 11.84 EE59 pKa = 4.21 KK60 pKa = 10.59 SFKK63 pKa = 10.14 SSSMVSQCDD72 pKa = 3.23 EE73 pKa = 4.67 DD74 pKa = 6.68 DD75 pKa = 3.77 IASQEE80 pKa = 4.1 MNKK83 pKa = 10.39 LEE85 pKa = 4.42 TIVNSACADD94 pKa = 3.62 EE95 pKa = 4.4 QQNIDD100 pKa = 3.74 DD101 pKa = 3.81 WNEE104 pKa = 3.71 YY105 pKa = 10.71 LEE107 pKa = 4.55 EE108 pKa = 4.14 NSGIKK113 pKa = 9.89 IMEE116 pKa = 4.53 GKK118 pKa = 10.55 VSTNEE123 pKa = 3.27 VDD125 pKa = 3.57 LNGVFEE131 pKa = 4.53 SKK133 pKa = 10.22 ILNRR137 pKa = 11.84 NSIINKK143 pKa = 9.71 DD144 pKa = 4.03 NIDD147 pKa = 3.65 SAVKK151 pKa = 10.32 KK152 pKa = 10.01 KK153 pKa = 11.21 ANINKK158 pKa = 8.62 MNMHH162 pKa = 6.67 DD163 pKa = 3.49 TSSDD167 pKa = 3.54 EE168 pKa = 4.1 EE169 pKa = 4.46 CNRR172 pKa = 11.84 NCKK175 pKa = 9.73 CCKK178 pKa = 9.85 KK179 pKa = 10.18 LRR181 pKa = 11.84 KK182 pKa = 9.04 LRR184 pKa = 11.84 KK185 pKa = 9.46 RR186 pKa = 11.84 MSILIAEE193 pKa = 4.76 SYY195 pKa = 10.62
Molecular weight: 22.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.61
IPC2_protein 4.533
IPC_protein 4.482
Toseland 4.291
ProMoST 4.596
Dawson 4.431
Bjellqvist 4.584
Wikipedia 4.329
Rodwell 4.304
Grimsley 4.202
Solomon 4.431
Lehninger 4.38
Nozaki 4.533
DTASelect 4.736
Thurlkill 4.317
EMBOSS 4.342
Sillero 4.584
Patrickios 4.037
IPC_peptide 4.431
IPC2_peptide 4.571
IPC2.peptide.svr19 4.538
Protein with the highest isoelectric point:
>tr|E2EBU0|E2EBU0_9REOV Inner capsid protein VP2 OS=Rotavirus D chicken/05V0049/DEU/2005 OX=884200 PE=3 SV=1
MM1 pKa = 7.01 GTYY4 pKa = 10.78 NLLLQKK10 pKa = 9.6 YY11 pKa = 8.78 LRR13 pKa = 11.84 WLYY16 pKa = 11.11 GKK18 pKa = 9.58 DD19 pKa = 2.94 ATVIPIFITNDD30 pKa = 3.14 QNNQEE35 pKa = 4.26 ILNSIVDD42 pKa = 3.79 YY43 pKa = 11.04 AKK45 pKa = 10.61 QLAIDD50 pKa = 4.13 DD51 pKa = 4.31 KK52 pKa = 11.46 YY53 pKa = 11.4 DD54 pKa = 3.36 YY55 pKa = 11.47 KK56 pKa = 11.17 LIEE59 pKa = 5.22 DD60 pKa = 4.71 KK61 pKa = 11.07 LSKK64 pKa = 10.98 CNFDD68 pKa = 3.66 YY69 pKa = 11.2 EE70 pKa = 4.41 IVSFFTYY77 pKa = 10.63 SDD79 pKa = 3.12 QKK81 pKa = 10.83 YY82 pKa = 11.14 DD83 pKa = 3.6 NVEE86 pKa = 3.94 QKK88 pKa = 10.68 LLKK91 pKa = 10.26 YY92 pKa = 10.1 VNKK95 pKa = 10.41 KK96 pKa = 9.74 KK97 pKa = 11.02 VLVADD102 pKa = 4.05 LTRR105 pKa = 11.84 NKK107 pKa = 10.33 LKK109 pKa = 11.05 YY110 pKa = 8.77 EE111 pKa = 3.95 NNYY114 pKa = 7.37 ITKK117 pKa = 10.34 DD118 pKa = 3.29 LYY120 pKa = 11.08 TEE122 pKa = 4.97 DD123 pKa = 5.74 DD124 pKa = 3.94 YY125 pKa = 11.89 TDD127 pKa = 3.45 TLMDD131 pKa = 4.3 PSINTSLRR139 pKa = 11.84 SNLNAAMFVIEE150 pKa = 4.73 NIEE153 pKa = 4.03 DD154 pKa = 3.44 KK155 pKa = 10.71 KK156 pKa = 9.84 IQSRR160 pKa = 11.84 YY161 pKa = 7.2 RR162 pKa = 11.84 HH163 pKa = 5.57 LFEE166 pKa = 4.84 YY167 pKa = 10.29 VASTVSHH174 pKa = 5.99 YY175 pKa = 10.49 GIPVHH180 pKa = 5.21 NQKK183 pKa = 9.88 YY184 pKa = 8.84 RR185 pKa = 11.84 YY186 pKa = 9.29 DD187 pKa = 3.71 YY188 pKa = 11.44 KK189 pKa = 11.35 EE190 pKa = 4.35 MYY192 pKa = 9.24 NKK194 pKa = 10.14 PYY196 pKa = 11.02 YY197 pKa = 9.89 LVPWANSSIEE207 pKa = 4.05 MIFAVSSHH215 pKa = 5.47 EE216 pKa = 4.15 LYY218 pKa = 10.87 LVAEE222 pKa = 4.21 EE223 pKa = 5.11 LIINSFSNRR232 pKa = 11.84 STLAKK237 pKa = 9.32 LTSSPMTTLTSKK249 pKa = 11.12 LDD251 pKa = 3.7 LYY253 pKa = 10.29 SCYY256 pKa = 8.38 MTTEE260 pKa = 4.21 DD261 pKa = 6.13 LEE263 pKa = 5.03 LEE265 pKa = 4.24 YY266 pKa = 10.84 SDD268 pKa = 5.93 KK269 pKa = 10.73 IVKK272 pKa = 10.24 SYY274 pKa = 9.05 ITDD277 pKa = 3.53 EE278 pKa = 4.36 AQLEE282 pKa = 4.23 LDD284 pKa = 3.7 EE285 pKa = 5.51 QIAEE289 pKa = 4.06 LRR291 pKa = 11.84 KK292 pKa = 10.28 EE293 pKa = 4.22 KK294 pKa = 8.37 MTKK297 pKa = 8.47 TADD300 pKa = 5.39 IIDD303 pKa = 3.45 EE304 pKa = 4.23 WQRR307 pKa = 11.84 NPSIDD312 pKa = 3.93 TFPLMAQIYY321 pKa = 9.28 SFSFHH326 pKa = 5.86 VGYY329 pKa = 9.99 RR330 pKa = 11.84 KK331 pKa = 10.3 QMMLDD336 pKa = 3.21 AVTDD340 pKa = 3.74 QLSIEE345 pKa = 4.23 YY346 pKa = 10.38 SDD348 pKa = 4.42 TFDD351 pKa = 4.1 KK352 pKa = 11.3 DD353 pKa = 3.37 MYY355 pKa = 11.77 NMYY358 pKa = 9.54 TNNIIKK364 pKa = 10.23 IIDD367 pKa = 3.76 DD368 pKa = 3.67 MLRR371 pKa = 11.84 DD372 pKa = 4.17 HH373 pKa = 7.15 IKK375 pKa = 10.79 KK376 pKa = 9.31 EE377 pKa = 4.37 DD378 pKa = 3.55 KK379 pKa = 10.18 MLLNAEE385 pKa = 4.15 IAGLLSMSSASNGEE399 pKa = 3.78 SRR401 pKa = 11.84 VIKK404 pKa = 10.36 FGRR407 pKa = 11.84 RR408 pKa = 11.84 TVRR411 pKa = 11.84 STKK414 pKa = 10.83 KK415 pKa = 10.29 NMHH418 pKa = 5.59 VMDD421 pKa = 6.45 DD422 pKa = 4.08 IFNKK426 pKa = 10.18 QYY428 pKa = 11.29 DD429 pKa = 4.25 LDD431 pKa = 4.18 VPDD434 pKa = 4.1 VNEE437 pKa = 4.4 NNPIPLGRR445 pKa = 11.84 RR446 pKa = 11.84 DD447 pKa = 3.43 VPGRR451 pKa = 11.84 RR452 pKa = 11.84 TRR454 pKa = 11.84 VIFILPYY461 pKa = 9.41 PYY463 pKa = 10.35 FIAQHH468 pKa = 5.56 SVVEE472 pKa = 4.38 LYY474 pKa = 10.78 LRR476 pKa = 11.84 EE477 pKa = 4.2 SKK479 pKa = 10.48 HH480 pKa = 6.13 IKK482 pKa = 9.82 EE483 pKa = 4.0 FSEE486 pKa = 5.59 FYY488 pKa = 10.47 SQSSQLLSYY497 pKa = 11.28 GDD499 pKa = 3.43 TNRR502 pKa = 11.84 YY503 pKa = 9.11 LNQSTIIVYY512 pKa = 10.72 ADD514 pKa = 3.11 VSQWDD519 pKa = 3.89 SSKK522 pKa = 11.38 HH523 pKa = 3.63 NTTPLRR529 pKa = 11.84 NAILKK534 pKa = 10.42 AIEE537 pKa = 3.94 QLKK540 pKa = 10.77 SYY542 pKa = 7.08 TTNEE546 pKa = 3.89 KK547 pKa = 10.91 VITALNNYY555 pKa = 9.97 ALTQMKK561 pKa = 9.97 LKK563 pKa = 10.79 NSFVTIGNKK572 pKa = 8.58 VLQYY576 pKa = 10.7 GAVASGEE583 pKa = 4.2 KK584 pKa = 7.8 QTKK587 pKa = 9.65 LMNSIANLALITTVINYY604 pKa = 9.8 LNVGEE609 pKa = 4.57 SYY611 pKa = 10.27 RR612 pKa = 11.84 IKK614 pKa = 10.41 IIRR617 pKa = 11.84 VDD619 pKa = 3.23 GDD621 pKa = 3.57 DD622 pKa = 4.13 NYY624 pKa = 11.28 FIMEE628 pKa = 4.75 FDD630 pKa = 3.67 RR631 pKa = 11.84 KK632 pKa = 10.01 VDD634 pKa = 3.53 KK635 pKa = 11.18 QLVTVVSNAVKK646 pKa = 9.42 DD647 pKa = 3.53 TYY649 pKa = 11.66 GRR651 pKa = 11.84 MDD653 pKa = 3.71 VKK655 pKa = 11.12 VKK657 pKa = 10.83 ALTATTGLEE666 pKa = 3.7 MAKK669 pKa = 10.03 RR670 pKa = 11.84 YY671 pKa = 9.19 IAGGRR676 pKa = 11.84 LFFRR680 pKa = 11.84 AGVNILNNEE689 pKa = 3.97 KK690 pKa = 9.28 RR691 pKa = 11.84 THH693 pKa = 4.91 TTTYY697 pKa = 9.86 DD698 pKa = 3.08 QAAIIYY704 pKa = 10.39 ANYY707 pKa = 9.82 LVNKK711 pKa = 8.05 MRR713 pKa = 11.84 GYY715 pKa = 9.58 NMEE718 pKa = 5.28 RR719 pKa = 11.84 IFILCKK725 pKa = 9.57 IMQMTSVKK733 pKa = 8.62 ITGSIRR739 pKa = 11.84 LFPASLMLTTNSPYY753 pKa = 10.77 KK754 pKa = 10.93 VFDD757 pKa = 4.42 DD758 pKa = 3.6 VDD760 pKa = 4.4 FVQTYY765 pKa = 9.8 NSSTIAIHH773 pKa = 5.12 LQKK776 pKa = 10.22 MLISVSQVKK785 pKa = 10.7 SNLADD790 pKa = 5.48 DD791 pKa = 3.8 IAKK794 pKa = 10.13 SEE796 pKa = 4.05 KK797 pKa = 8.34 FTNYY801 pKa = 9.3 VQFLTKK807 pKa = 10.39 KK808 pKa = 10.68 LLVHH812 pKa = 6.14 NNKK815 pKa = 9.29 IVEE818 pKa = 4.07 MGIARR823 pKa = 11.84 TEE825 pKa = 3.97 KK826 pKa = 10.71 AKK828 pKa = 10.93 LNSYY832 pKa = 9.89 PPIANEE838 pKa = 4.31 KK839 pKa = 10.45 RR840 pKa = 11.84 NNQLKK845 pKa = 8.53 TLMTFLQTPTYY856 pKa = 10.82 KK857 pKa = 10.04 MSTDD861 pKa = 3.27 VTINDD866 pKa = 3.16 MMKK869 pKa = 10.22 IINEE873 pKa = 4.09 YY874 pKa = 9.51 TNYY877 pKa = 10.51 TIITDD882 pKa = 3.59 KK883 pKa = 11.41 YY884 pKa = 9.48 GIQPNPMPLLPEE896 pKa = 4.82 NIQFAMSHH904 pKa = 4.95 VGARR908 pKa = 11.84 VYY910 pKa = 10.6 QIEE913 pKa = 4.06 EE914 pKa = 3.9 SAARR918 pKa = 11.84 SAISKK923 pKa = 9.84 LISNYY928 pKa = 7.6 TVYY931 pKa = 10.58 KK932 pKa = 10.04 PSVDD936 pKa = 3.37 EE937 pKa = 4.38 LYY939 pKa = 10.92 KK940 pKa = 10.81 VINSNEE946 pKa = 4.18 RR947 pKa = 11.84 ILFEE951 pKa = 4.25 YY952 pKa = 8.62 VTSFGVPTRR961 pKa = 11.84 DD962 pKa = 2.92 VTAYY966 pKa = 10.72 LSAKK970 pKa = 9.98 LYY972 pKa = 10.89 KK973 pKa = 9.66 KK974 pKa = 10.35 DD975 pKa = 3.52 RR976 pKa = 11.84 NLILEE981 pKa = 4.23 SYY983 pKa = 9.41 IYY985 pKa = 10.65 QIMSVNYY992 pKa = 9.74 NAYY995 pKa = 10.29 QLFNLNSEE1003 pKa = 4.85 LFDD1006 pKa = 3.74 KK1007 pKa = 10.85 YY1008 pKa = 11.21 INIVTYY1014 pKa = 9.49 MKK1016 pKa = 10.18 IPSVNFIIFTYY1027 pKa = 10.3 LKK1029 pKa = 10.75 LVILNKK1035 pKa = 9.5 MLQEE1039 pKa = 4.09 RR1040 pKa = 11.84 KK1041 pKa = 9.62 LYY1043 pKa = 10.19 KK1044 pKa = 10.15 AYY1046 pKa = 10.6 CQIPKK1051 pKa = 10.03 HH1052 pKa = 5.83 LLHH1055 pKa = 6.89 ILWKK1059 pKa = 9.92 MSINLATISSPYY1071 pKa = 9.2 TIANFFQEE1079 pKa = 4.12
Molecular weight: 125.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.062
IPC2_protein 8.229
IPC_protein 8.083
Toseland 8.58
ProMoST 8.551
Dawson 8.96
Bjellqvist 8.785
Wikipedia 9.165
Rodwell 9.077
Grimsley 9.048
Solomon 9.004
Lehninger 8.946
Nozaki 8.697
DTASelect 8.741
Thurlkill 8.799
EMBOSS 9.063
Sillero 8.946
Patrickios 4.05
IPC_peptide 8.99
IPC2_peptide 7.366
IPC2.peptide.svr19 7.563
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12
0
12
5837
93
1079
486.4
56.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.985 ± 0.528
1.285 ± 0.432
6.476 ± 0.295
5.362 ± 0.333
4.095 ± 0.261
3.135 ± 0.332
1.559 ± 0.24
8.103 ± 0.382
6.39 ± 0.648
9.56 ± 0.247
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.964 ± 0.35
7.487 ± 0.335
2.861 ± 0.423
3.701 ± 0.23
4.934 ± 0.426
7.949 ± 0.432
6.664 ± 0.411
6.339 ± 0.29
0.685 ± 0.117
5.465 ± 0.447
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here