Rotavirus D chicken/05V0049/DEU/2005

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Rotavirus; Rotavirus D

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|E2EBV0|E2EBV0_9REOV NSP5 OS=Rotavirus D chicken/05V0049/DEU/2005 OX=884200 PE=4 SV=1
MM1 pKa = 7.57MDD3 pKa = 4.62DD4 pKa = 4.51LDD6 pKa = 5.88FNFEE10 pKa = 4.51SNLPEE15 pKa = 4.44ISLISSRR22 pKa = 11.84AGTTYY27 pKa = 10.73TKK29 pKa = 9.8IDD31 pKa = 3.75YY32 pKa = 10.86DD33 pKa = 4.1EE34 pKa = 6.2DD35 pKa = 3.73MLLDD39 pKa = 5.8DD40 pKa = 4.82ITPSDD45 pKa = 3.88SASSQDD51 pKa = 3.19TNQRR55 pKa = 11.84TFRR58 pKa = 11.84EE59 pKa = 4.21KK60 pKa = 10.59SFKK63 pKa = 10.14SSSMVSQCDD72 pKa = 3.23EE73 pKa = 4.67DD74 pKa = 6.68DD75 pKa = 3.77IASQEE80 pKa = 4.1MNKK83 pKa = 10.39LEE85 pKa = 4.42TIVNSACADD94 pKa = 3.62EE95 pKa = 4.4QQNIDD100 pKa = 3.74DD101 pKa = 3.81WNEE104 pKa = 3.71YY105 pKa = 10.71LEE107 pKa = 4.55EE108 pKa = 4.14NSGIKK113 pKa = 9.89IMEE116 pKa = 4.53GKK118 pKa = 10.55VSTNEE123 pKa = 3.27VDD125 pKa = 3.57LNGVFEE131 pKa = 4.53SKK133 pKa = 10.22ILNRR137 pKa = 11.84NSIINKK143 pKa = 9.71DD144 pKa = 4.03NIDD147 pKa = 3.65SAVKK151 pKa = 10.32KK152 pKa = 10.01KK153 pKa = 11.21ANINKK158 pKa = 8.62MNMHH162 pKa = 6.67DD163 pKa = 3.49TSSDD167 pKa = 3.54EE168 pKa = 4.1EE169 pKa = 4.46CNRR172 pKa = 11.84NCKK175 pKa = 9.73CCKK178 pKa = 9.85KK179 pKa = 10.18LRR181 pKa = 11.84KK182 pKa = 9.04LRR184 pKa = 11.84KK185 pKa = 9.46RR186 pKa = 11.84MSILIAEE193 pKa = 4.76SYY195 pKa = 10.62

Molecular weight:
22.26 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|E2EBU0|E2EBU0_9REOV Inner capsid protein VP2 OS=Rotavirus D chicken/05V0049/DEU/2005 OX=884200 PE=3 SV=1
MM1 pKa = 7.01GTYY4 pKa = 10.78NLLLQKK10 pKa = 9.6YY11 pKa = 8.78LRR13 pKa = 11.84WLYY16 pKa = 11.11GKK18 pKa = 9.58DD19 pKa = 2.94ATVIPIFITNDD30 pKa = 3.14QNNQEE35 pKa = 4.26ILNSIVDD42 pKa = 3.79YY43 pKa = 11.04AKK45 pKa = 10.61QLAIDD50 pKa = 4.13DD51 pKa = 4.31KK52 pKa = 11.46YY53 pKa = 11.4DD54 pKa = 3.36YY55 pKa = 11.47KK56 pKa = 11.17LIEE59 pKa = 5.22DD60 pKa = 4.71KK61 pKa = 11.07LSKK64 pKa = 10.98CNFDD68 pKa = 3.66YY69 pKa = 11.2EE70 pKa = 4.41IVSFFTYY77 pKa = 10.63SDD79 pKa = 3.12QKK81 pKa = 10.83YY82 pKa = 11.14DD83 pKa = 3.6NVEE86 pKa = 3.94QKK88 pKa = 10.68LLKK91 pKa = 10.26YY92 pKa = 10.1VNKK95 pKa = 10.41KK96 pKa = 9.74KK97 pKa = 11.02VLVADD102 pKa = 4.05LTRR105 pKa = 11.84NKK107 pKa = 10.33LKK109 pKa = 11.05YY110 pKa = 8.77EE111 pKa = 3.95NNYY114 pKa = 7.37ITKK117 pKa = 10.34DD118 pKa = 3.29LYY120 pKa = 11.08TEE122 pKa = 4.97DD123 pKa = 5.74DD124 pKa = 3.94YY125 pKa = 11.89TDD127 pKa = 3.45TLMDD131 pKa = 4.3PSINTSLRR139 pKa = 11.84SNLNAAMFVIEE150 pKa = 4.73NIEE153 pKa = 4.03DD154 pKa = 3.44KK155 pKa = 10.71KK156 pKa = 9.84IQSRR160 pKa = 11.84YY161 pKa = 7.2RR162 pKa = 11.84HH163 pKa = 5.57LFEE166 pKa = 4.84YY167 pKa = 10.29VASTVSHH174 pKa = 5.99YY175 pKa = 10.49GIPVHH180 pKa = 5.21NQKK183 pKa = 9.88YY184 pKa = 8.84RR185 pKa = 11.84YY186 pKa = 9.29DD187 pKa = 3.71YY188 pKa = 11.44KK189 pKa = 11.35EE190 pKa = 4.35MYY192 pKa = 9.24NKK194 pKa = 10.14PYY196 pKa = 11.02YY197 pKa = 9.89LVPWANSSIEE207 pKa = 4.05MIFAVSSHH215 pKa = 5.47EE216 pKa = 4.15LYY218 pKa = 10.87LVAEE222 pKa = 4.21EE223 pKa = 5.11LIINSFSNRR232 pKa = 11.84STLAKK237 pKa = 9.32LTSSPMTTLTSKK249 pKa = 11.12LDD251 pKa = 3.7LYY253 pKa = 10.29SCYY256 pKa = 8.38MTTEE260 pKa = 4.21DD261 pKa = 6.13LEE263 pKa = 5.03LEE265 pKa = 4.24YY266 pKa = 10.84SDD268 pKa = 5.93KK269 pKa = 10.73IVKK272 pKa = 10.24SYY274 pKa = 9.05ITDD277 pKa = 3.53EE278 pKa = 4.36AQLEE282 pKa = 4.23LDD284 pKa = 3.7EE285 pKa = 5.51QIAEE289 pKa = 4.06LRR291 pKa = 11.84KK292 pKa = 10.28EE293 pKa = 4.22KK294 pKa = 8.37MTKK297 pKa = 8.47TADD300 pKa = 5.39IIDD303 pKa = 3.45EE304 pKa = 4.23WQRR307 pKa = 11.84NPSIDD312 pKa = 3.93TFPLMAQIYY321 pKa = 9.28SFSFHH326 pKa = 5.86VGYY329 pKa = 9.99RR330 pKa = 11.84KK331 pKa = 10.3QMMLDD336 pKa = 3.21AVTDD340 pKa = 3.74QLSIEE345 pKa = 4.23YY346 pKa = 10.38SDD348 pKa = 4.42TFDD351 pKa = 4.1KK352 pKa = 11.3DD353 pKa = 3.37MYY355 pKa = 11.77NMYY358 pKa = 9.54TNNIIKK364 pKa = 10.23IIDD367 pKa = 3.76DD368 pKa = 3.67MLRR371 pKa = 11.84DD372 pKa = 4.17HH373 pKa = 7.15IKK375 pKa = 10.79KK376 pKa = 9.31EE377 pKa = 4.37DD378 pKa = 3.55KK379 pKa = 10.18MLLNAEE385 pKa = 4.15IAGLLSMSSASNGEE399 pKa = 3.78SRR401 pKa = 11.84VIKK404 pKa = 10.36FGRR407 pKa = 11.84RR408 pKa = 11.84TVRR411 pKa = 11.84STKK414 pKa = 10.83KK415 pKa = 10.29NMHH418 pKa = 5.59VMDD421 pKa = 6.45DD422 pKa = 4.08IFNKK426 pKa = 10.18QYY428 pKa = 11.29DD429 pKa = 4.25LDD431 pKa = 4.18VPDD434 pKa = 4.1VNEE437 pKa = 4.4NNPIPLGRR445 pKa = 11.84RR446 pKa = 11.84DD447 pKa = 3.43VPGRR451 pKa = 11.84RR452 pKa = 11.84TRR454 pKa = 11.84VIFILPYY461 pKa = 9.41PYY463 pKa = 10.35FIAQHH468 pKa = 5.56SVVEE472 pKa = 4.38LYY474 pKa = 10.78LRR476 pKa = 11.84EE477 pKa = 4.2SKK479 pKa = 10.48HH480 pKa = 6.13IKK482 pKa = 9.82EE483 pKa = 4.0FSEE486 pKa = 5.59FYY488 pKa = 10.47SQSSQLLSYY497 pKa = 11.28GDD499 pKa = 3.43TNRR502 pKa = 11.84YY503 pKa = 9.11LNQSTIIVYY512 pKa = 10.72ADD514 pKa = 3.11VSQWDD519 pKa = 3.89SSKK522 pKa = 11.38HH523 pKa = 3.63NTTPLRR529 pKa = 11.84NAILKK534 pKa = 10.42AIEE537 pKa = 3.94QLKK540 pKa = 10.77SYY542 pKa = 7.08TTNEE546 pKa = 3.89KK547 pKa = 10.91VITALNNYY555 pKa = 9.97ALTQMKK561 pKa = 9.97LKK563 pKa = 10.79NSFVTIGNKK572 pKa = 8.58VLQYY576 pKa = 10.7GAVASGEE583 pKa = 4.2KK584 pKa = 7.8QTKK587 pKa = 9.65LMNSIANLALITTVINYY604 pKa = 9.8LNVGEE609 pKa = 4.57SYY611 pKa = 10.27RR612 pKa = 11.84IKK614 pKa = 10.41IIRR617 pKa = 11.84VDD619 pKa = 3.23GDD621 pKa = 3.57DD622 pKa = 4.13NYY624 pKa = 11.28FIMEE628 pKa = 4.75FDD630 pKa = 3.67RR631 pKa = 11.84KK632 pKa = 10.01VDD634 pKa = 3.53KK635 pKa = 11.18QLVTVVSNAVKK646 pKa = 9.42DD647 pKa = 3.53TYY649 pKa = 11.66GRR651 pKa = 11.84MDD653 pKa = 3.71VKK655 pKa = 11.12VKK657 pKa = 10.83ALTATTGLEE666 pKa = 3.7MAKK669 pKa = 10.03RR670 pKa = 11.84YY671 pKa = 9.19IAGGRR676 pKa = 11.84LFFRR680 pKa = 11.84AGVNILNNEE689 pKa = 3.97KK690 pKa = 9.28RR691 pKa = 11.84THH693 pKa = 4.91TTTYY697 pKa = 9.86DD698 pKa = 3.08QAAIIYY704 pKa = 10.39ANYY707 pKa = 9.82LVNKK711 pKa = 8.05MRR713 pKa = 11.84GYY715 pKa = 9.58NMEE718 pKa = 5.28RR719 pKa = 11.84IFILCKK725 pKa = 9.57IMQMTSVKK733 pKa = 8.62ITGSIRR739 pKa = 11.84LFPASLMLTTNSPYY753 pKa = 10.77KK754 pKa = 10.93VFDD757 pKa = 4.42DD758 pKa = 3.6VDD760 pKa = 4.4FVQTYY765 pKa = 9.8NSSTIAIHH773 pKa = 5.12LQKK776 pKa = 10.22MLISVSQVKK785 pKa = 10.7SNLADD790 pKa = 5.48DD791 pKa = 3.8IAKK794 pKa = 10.13SEE796 pKa = 4.05KK797 pKa = 8.34FTNYY801 pKa = 9.3VQFLTKK807 pKa = 10.39KK808 pKa = 10.68LLVHH812 pKa = 6.14NNKK815 pKa = 9.29IVEE818 pKa = 4.07MGIARR823 pKa = 11.84TEE825 pKa = 3.97KK826 pKa = 10.71AKK828 pKa = 10.93LNSYY832 pKa = 9.89PPIANEE838 pKa = 4.31KK839 pKa = 10.45RR840 pKa = 11.84NNQLKK845 pKa = 8.53TLMTFLQTPTYY856 pKa = 10.82KK857 pKa = 10.04MSTDD861 pKa = 3.27VTINDD866 pKa = 3.16MMKK869 pKa = 10.22IINEE873 pKa = 4.09YY874 pKa = 9.51TNYY877 pKa = 10.51TIITDD882 pKa = 3.59KK883 pKa = 11.41YY884 pKa = 9.48GIQPNPMPLLPEE896 pKa = 4.82NIQFAMSHH904 pKa = 4.95VGARR908 pKa = 11.84VYY910 pKa = 10.6QIEE913 pKa = 4.06EE914 pKa = 3.9SAARR918 pKa = 11.84SAISKK923 pKa = 9.84LISNYY928 pKa = 7.6TVYY931 pKa = 10.58KK932 pKa = 10.04PSVDD936 pKa = 3.37EE937 pKa = 4.38LYY939 pKa = 10.92KK940 pKa = 10.81VINSNEE946 pKa = 4.18RR947 pKa = 11.84ILFEE951 pKa = 4.25YY952 pKa = 8.62VTSFGVPTRR961 pKa = 11.84DD962 pKa = 2.92VTAYY966 pKa = 10.72LSAKK970 pKa = 9.98LYY972 pKa = 10.89KK973 pKa = 9.66KK974 pKa = 10.35DD975 pKa = 3.52RR976 pKa = 11.84NLILEE981 pKa = 4.23SYY983 pKa = 9.41IYY985 pKa = 10.65QIMSVNYY992 pKa = 9.74NAYY995 pKa = 10.29QLFNLNSEE1003 pKa = 4.85LFDD1006 pKa = 3.74KK1007 pKa = 10.85YY1008 pKa = 11.21INIVTYY1014 pKa = 9.49MKK1016 pKa = 10.18IPSVNFIIFTYY1027 pKa = 10.3LKK1029 pKa = 10.75LVILNKK1035 pKa = 9.5MLQEE1039 pKa = 4.09RR1040 pKa = 11.84KK1041 pKa = 9.62LYY1043 pKa = 10.19KK1044 pKa = 10.15AYY1046 pKa = 10.6CQIPKK1051 pKa = 10.03HH1052 pKa = 5.83LLHH1055 pKa = 6.89ILWKK1059 pKa = 9.92MSINLATISSPYY1071 pKa = 9.2TIANFFQEE1079 pKa = 4.12

Molecular weight:
125.44 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

5837

93

1079

486.4

56.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.985 ± 0.528

1.285 ± 0.432

6.476 ± 0.295

5.362 ± 0.333

4.095 ± 0.261

3.135 ± 0.332

1.559 ± 0.24

8.103 ± 0.382

6.39 ± 0.648

9.56 ± 0.247

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.964 ± 0.35

7.487 ± 0.335

2.861 ± 0.423

3.701 ± 0.23

4.934 ± 0.426

7.949 ± 0.432

6.664 ± 0.411

6.339 ± 0.29

0.685 ± 0.117

5.465 ± 0.447

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski