Ornithinimicrobium humiphilum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Ornithinimicrobiaceae; Ornithinimicrobium

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3155 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A543KKB5|A0A543KKB5_9MICO Uncharacterized protein OS=Ornithinimicrobium humiphilum OX=125288 GN=FB476_0366 PE=4 SV=1
MM1 pKa = 7.36SRR3 pKa = 11.84TAGVVSGVAFGAMALTGCGGGATDD27 pKa = 4.27EE28 pKa = 4.44AAQAAYY34 pKa = 9.34DD35 pKa = 3.6ACADD39 pKa = 4.02SEE41 pKa = 4.27ADD43 pKa = 3.35APLFRR48 pKa = 11.84LDD50 pKa = 3.21GDD52 pKa = 4.32TVHH55 pKa = 6.99VEE57 pKa = 4.15LKK59 pKa = 10.61GDD61 pKa = 3.66NARR64 pKa = 11.84QLGNMPDD71 pKa = 3.07IAGQIGTDD79 pKa = 3.78PQDD82 pKa = 3.15MDD84 pKa = 4.0LSGFGVGLALLAGLDD99 pKa = 4.04CMSEE103 pKa = 4.02HH104 pKa = 6.54TGFPGASSDD113 pKa = 5.16LEE115 pKa = 5.86AGDD118 pKa = 3.91EE119 pKa = 4.37WEE121 pKa = 4.32GWRR124 pKa = 11.84YY125 pKa = 7.93TYY127 pKa = 10.91EE128 pKa = 4.09SGAGSEE134 pKa = 4.36EE135 pKa = 4.3THH137 pKa = 6.62SFTATSS143 pKa = 3.32

Molecular weight: 14.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A543KJE6|A0A543KJE6_9MICO DNA gyrase subunit B OS=Ornithinimicrobium humiphilum OX=125288 GN=gyrB PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.97KK16 pKa = 9.33HH17 pKa = 4.25GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.63GRR40 pKa = 11.84SSISAA45 pKa = 3.4

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3155

0

3155

1044644

29

2071

331.1

35.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.043 ± 0.053

0.636 ± 0.01

6.331 ± 0.036

6.265 ± 0.045

2.458 ± 0.028

9.476 ± 0.039

2.197 ± 0.022

3.03 ± 0.035

1.515 ± 0.029

10.644 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.844 ± 0.018

1.478 ± 0.024

5.978 ± 0.036

2.785 ± 0.024

8.001 ± 0.055

4.917 ± 0.027

6.098 ± 0.031

9.92 ± 0.047

1.578 ± 0.018

1.807 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski