Bacillus phage Mgbh1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Magadivirus; Bacillus virus Mgbh1

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A142F1S8|A0A142F1S8_9CAUD YorM-like protein OS=Bacillus phage Mgbh1 OX=1796993 PE=4 SV=1
MM1 pKa = 7.53TYY3 pKa = 10.43YY4 pKa = 10.81FEE6 pKa = 5.74FPDD9 pKa = 3.46HH10 pKa = 6.92EE11 pKa = 4.81YY12 pKa = 10.42YY13 pKa = 11.31ALVAVDD19 pKa = 4.07TNSFTQAAEE28 pKa = 4.0IYY30 pKa = 9.89AKK32 pKa = 10.72YY33 pKa = 10.19IADD36 pKa = 4.29DD37 pKa = 4.09GVLSVLEE44 pKa = 4.23EE45 pKa = 4.27GSPVFLTEE53 pKa = 4.14AEE55 pKa = 3.84AFMRR59 pKa = 11.84FMSANDD65 pKa = 3.72SQNMTVEE72 pKa = 3.83QATDD76 pKa = 2.84KK77 pKa = 11.24FYY79 pKa = 10.14DD80 pKa = 3.66TKK82 pKa = 11.36NGVLLIDD89 pKa = 3.91SALLL93 pKa = 3.68

Molecular weight: 10.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A142F1N8|A0A142F1N8_9CAUD HTH_17 domain-containing protein OS=Bacillus phage Mgbh1 OX=1796993 PE=4 SV=1
MM1 pKa = 7.82KK2 pKa = 10.28IHH4 pKa = 7.61DD5 pKa = 4.9LRR7 pKa = 11.84KK8 pKa = 8.04QTPKK12 pKa = 10.67RR13 pKa = 11.84STTRR17 pKa = 11.84PISAITKK24 pKa = 9.34IVRR27 pKa = 11.84HH28 pKa = 6.04HH29 pKa = 6.4SATTGGDD36 pKa = 4.14FWAFWNGRR44 pKa = 11.84WKK46 pKa = 10.97GSGWKK51 pKa = 8.48TGGYY55 pKa = 9.89HH56 pKa = 7.39EE57 pKa = 5.7IILRR61 pKa = 11.84DD62 pKa = 3.64GSVQLCYY69 pKa = 10.85DD70 pKa = 3.32PTMATNGVLDD80 pKa = 4.15HH81 pKa = 7.11NSYY84 pKa = 9.52TYY86 pKa = 9.98HH87 pKa = 6.38ICVVGNGQFTEE98 pKa = 4.28AQEE101 pKa = 4.0KK102 pKa = 8.67TFDD105 pKa = 3.78VRR107 pKa = 11.84AKK109 pKa = 10.62LAMEE113 pKa = 4.49RR114 pKa = 11.84FGLKK118 pKa = 10.26AGDD121 pKa = 3.67VVGHH125 pKa = 6.03RR126 pKa = 11.84EE127 pKa = 3.79IPGAATEE134 pKa = 4.31CPGINMNTVRR144 pKa = 11.84ARR146 pKa = 11.84LVGAKK151 pKa = 10.0VEE153 pKa = 4.32TPKK156 pKa = 10.67TEE158 pKa = 4.14IKK160 pKa = 10.88NEE162 pKa = 4.12TVKK165 pKa = 10.19TSKK168 pKa = 10.16PKK170 pKa = 10.73SSTSPYY176 pKa = 10.92DD177 pKa = 3.71LIRR180 pKa = 11.84RR181 pKa = 11.84YY182 pKa = 9.63FPNVSVLQRR191 pKa = 11.84QLIAVGEE198 pKa = 4.12NLSRR202 pKa = 11.84FRR204 pKa = 11.84DD205 pKa = 3.67DD206 pKa = 4.7GVPGDD211 pKa = 3.91EE212 pKa = 4.36TLNAIKK218 pKa = 10.47SFQRR222 pKa = 11.84KK223 pKa = 7.66EE224 pKa = 3.79RR225 pKa = 11.84LTVDD229 pKa = 4.87GIPGPKK235 pKa = 7.74TQARR239 pKa = 11.84LRR241 pKa = 11.84SKK243 pKa = 10.07IRR245 pKa = 11.84YY246 pKa = 8.53VRR248 pKa = 11.84LLRR251 pKa = 11.84LMNPRR256 pKa = 11.84LRR258 pKa = 11.84GNDD261 pKa = 2.7VRR263 pKa = 11.84LIQRR267 pKa = 11.84VLGVTADD274 pKa = 4.4GIFGPITQSAVRR286 pKa = 11.84EE287 pKa = 4.21YY288 pKa = 10.57QRR290 pKa = 11.84KK291 pKa = 8.42HH292 pKa = 5.43RR293 pKa = 11.84LKK295 pKa = 10.01VDD297 pKa = 4.62GIVGPQTWSHH307 pKa = 6.29LFGG310 pKa = 5.46

Molecular weight: 34.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

80

0

80

16985

45

1363

212.3

24.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.818 ± 0.359

0.824 ± 0.14

6.947 ± 0.329

8.525 ± 0.462

3.797 ± 0.266

6.629 ± 0.268

1.684 ± 0.136

6.606 ± 0.24

6.541 ± 0.309

7.73 ± 0.202

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.567 ± 0.192

4.634 ± 0.214

2.826 ± 0.166

3.209 ± 0.2

6.417 ± 0.27

5.935 ± 0.326

5.899 ± 0.272

7.147 ± 0.27

1.178 ± 0.106

4.086 ± 0.288
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski