Amino acid dipepetide frequency for Bacillus phage Mgbh1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.592AlaAla: 3.592 ± 0.654
0.589AlaCys: 0.589 ± 0.193
4.475AlaAsp: 4.475 ± 0.532
6.3AlaGlu: 6.3 ± 0.648
2.12AlaPhe: 2.12 ± 0.277
4.18AlaGly: 4.18 ± 0.665
1.472AlaHis: 1.472 ± 0.305
3.415AlaIle: 3.415 ± 0.516
5.358AlaLys: 5.358 ± 0.567
5.829AlaLeu: 5.829 ± 0.641
2.296AlaMet: 2.296 ± 0.441
3.945AlaAsn: 3.945 ± 0.603
1.531AlaPro: 1.531 ± 0.283
2.473AlaGln: 2.473 ± 0.376
3.592AlaArg: 3.592 ± 0.455
3.179AlaSer: 3.179 ± 0.446
3.709AlaThr: 3.709 ± 0.6
6.418AlaVal: 6.418 ± 0.804
1.001AlaTrp: 1.001 ± 0.272
2.65AlaTyr: 2.65 ± 0.377
0.0AlaXaa: 0.0 ± 0.0
Cys
0.412CysAla: 0.412 ± 0.157
0.118CysCys: 0.118 ± 0.082
0.707CysAsp: 0.707 ± 0.213
0.648CysGlu: 0.648 ± 0.225
0.177CysPhe: 0.177 ± 0.101
0.883CysGly: 0.883 ± 0.357
0.059CysHis: 0.059 ± 0.065
0.589CysIle: 0.589 ± 0.146
0.589CysLys: 0.589 ± 0.214
0.53CysLeu: 0.53 ± 0.16
0.059CysMet: 0.059 ± 0.067
0.236CysAsn: 0.236 ± 0.116
0.648CysPro: 0.648 ± 0.189
0.118CysGln: 0.118 ± 0.08
0.294CysArg: 0.294 ± 0.144
0.471CysSer: 0.471 ± 0.206
0.236CysThr: 0.236 ± 0.141
0.765CysVal: 0.765 ± 0.191
0.236CysTrp: 0.236 ± 0.126
0.471CysTyr: 0.471 ± 0.175
0.0CysXaa: 0.0 ± 0.0
Asp
4.71AspAla: 4.71 ± 0.652
0.648AspCys: 0.648 ± 0.191
4.475AspAsp: 4.475 ± 0.632
6.359AspGlu: 6.359 ± 0.751
3.356AspPhe: 3.356 ± 0.474
6.065AspGly: 6.065 ± 0.779
0.824AspHis: 0.824 ± 0.208
4.769AspIle: 4.769 ± 0.662
3.886AspLys: 3.886 ± 0.569
5.77AspLeu: 5.77 ± 0.581
1.884AspMet: 1.884 ± 0.276
2.237AspAsn: 2.237 ± 0.367
2.355AspPro: 2.355 ± 0.478
2.12AspGln: 2.12 ± 0.397
3.886AspArg: 3.886 ± 0.478
3.179AspSer: 3.179 ± 0.326
3.65AspThr: 3.65 ± 0.457
5.122AspVal: 5.122 ± 0.575
0.942AspTrp: 0.942 ± 0.29
3.238AspTyr: 3.238 ± 0.559
0.0AspXaa: 0.0 ± 0.0
Glu
5.888GluAla: 5.888 ± 0.57
1.001GluCys: 1.001 ± 0.285
5.947GluAsp: 5.947 ± 0.657
7.89GluGlu: 7.89 ± 0.857
2.65GluPhe: 2.65 ± 0.447
4.71GluGly: 4.71 ± 0.643
1.59GluHis: 1.59 ± 0.236
6.653GluIle: 6.653 ± 0.732
5.476GluLys: 5.476 ± 0.541
6.653GluLeu: 6.653 ± 0.809
2.532GluMet: 2.532 ± 0.342
3.592GluAsn: 3.592 ± 0.441
2.355GluPro: 2.355 ± 0.428
2.414GluGln: 2.414 ± 0.444
5.476GluArg: 5.476 ± 0.642
4.534GluSer: 4.534 ± 0.545
4.769GluThr: 4.769 ± 0.573
5.888GluVal: 5.888 ± 0.664
1.236GluTrp: 1.236 ± 0.265
4.004GluTyr: 4.004 ± 0.617
0.0GluXaa: 0.0 ± 0.0
Phe
2.179PheAla: 2.179 ± 0.368
0.0PheCys: 0.0 ± 0.0
3.003PheAsp: 3.003 ± 0.506
2.826PheGlu: 2.826 ± 0.315
1.178PhePhe: 1.178 ± 0.258
3.121PheGly: 3.121 ± 0.415
0.942PheHis: 0.942 ± 0.27
2.532PheIle: 2.532 ± 0.38
2.65PheLys: 2.65 ± 0.356
2.355PheLeu: 2.355 ± 0.431
0.824PheMet: 0.824 ± 0.253
1.649PheAsn: 1.649 ± 0.371
1.06PhePro: 1.06 ± 0.25
0.883PheGln: 0.883 ± 0.238
2.473PheArg: 2.473 ± 0.451
3.179PheSer: 3.179 ± 0.475
2.414PheThr: 2.414 ± 0.4
2.237PheVal: 2.237 ± 0.313
0.471PheTrp: 0.471 ± 0.185
2.002PheTyr: 2.002 ± 0.444
0.0PheXaa: 0.0 ± 0.0
Gly
3.415GlyAla: 3.415 ± 0.566
0.471GlyCys: 0.471 ± 0.16
5.417GlyAsp: 5.417 ± 0.658
6.536GlyGlu: 6.536 ± 0.676
2.826GlyPhe: 2.826 ± 0.483
4.593GlyGly: 4.593 ± 0.54
0.765GlyHis: 0.765 ± 0.256
4.71GlyIle: 4.71 ± 0.625
4.18GlyLys: 4.18 ± 0.562
5.593GlyLeu: 5.593 ± 0.68
1.766GlyMet: 1.766 ± 0.505
3.179GlyAsn: 3.179 ± 0.375
0.942GlyPro: 0.942 ± 0.247
1.884GlyGln: 1.884 ± 0.309
3.886GlyArg: 3.886 ± 0.532
3.356GlySer: 3.356 ± 0.511
3.592GlyThr: 3.592 ± 0.65
6.123GlyVal: 6.123 ± 0.511
0.824GlyTrp: 0.824 ± 0.221
2.237GlyTyr: 2.237 ± 0.342
0.0GlyXaa: 0.0 ± 0.0
His
1.354HisAla: 1.354 ± 0.257
0.059HisCys: 0.059 ± 0.056
1.178HisAsp: 1.178 ± 0.285
1.59HisGlu: 1.59 ± 0.353
0.294HisPhe: 0.294 ± 0.148
1.295HisGly: 1.295 ± 0.257
0.648HisHis: 0.648 ± 0.18
1.119HisIle: 1.119 ± 0.227
1.119HisLys: 1.119 ± 0.262
1.295HisLeu: 1.295 ± 0.287
0.236HisMet: 0.236 ± 0.116
0.765HisAsn: 0.765 ± 0.194
0.471HisPro: 0.471 ± 0.194
0.412HisGln: 0.412 ± 0.131
1.119HisArg: 1.119 ± 0.282
0.765HisSer: 0.765 ± 0.183
1.119HisThr: 1.119 ± 0.319
1.001HisVal: 1.001 ± 0.249
0.236HisTrp: 0.236 ± 0.149
0.765HisTyr: 0.765 ± 0.18
0.0HisXaa: 0.0 ± 0.0
Ile
5.652IleAla: 5.652 ± 0.525
0.412IleCys: 0.412 ± 0.139
5.24IleAsp: 5.24 ± 0.718
6.594IleGlu: 6.594 ± 0.857
1.472IlePhe: 1.472 ± 0.351
4.298IleGly: 4.298 ± 0.629
1.178IleHis: 1.178 ± 0.291
3.415IleIle: 3.415 ± 0.448
3.886IleLys: 3.886 ± 0.439
3.886IleLeu: 3.886 ± 0.46
1.413IleMet: 1.413 ± 0.28
3.592IleAsn: 3.592 ± 0.641
2.179IlePro: 2.179 ± 0.299
2.355IleGln: 2.355 ± 0.301
4.828IleArg: 4.828 ± 0.559
4.122IleSer: 4.122 ± 0.588
4.239IleThr: 4.239 ± 0.545
4.887IleVal: 4.887 ± 0.596
0.53IleTrp: 0.53 ± 0.181
1.884IleTyr: 1.884 ± 0.368
0.0IleXaa: 0.0 ± 0.0
Lys
4.357LysAla: 4.357 ± 0.594
0.471LysCys: 0.471 ± 0.156
4.122LysAsp: 4.122 ± 0.583
4.593LysGlu: 4.593 ± 0.668
2.708LysPhe: 2.708 ± 0.401
3.886LysGly: 3.886 ± 0.514
1.236LysHis: 1.236 ± 0.218
4.71LysIle: 4.71 ± 0.869
3.827LysLys: 3.827 ± 0.583
4.828LysLeu: 4.828 ± 0.537
1.59LysMet: 1.59 ± 0.349
2.179LysAsn: 2.179 ± 0.302
2.296LysPro: 2.296 ± 0.362
2.65LysGln: 2.65 ± 0.347
5.829LysArg: 5.829 ± 0.726
3.886LysSer: 3.886 ± 0.508
4.357LysThr: 4.357 ± 0.745
4.357LysVal: 4.357 ± 0.752
0.883LysTrp: 0.883 ± 0.186
2.65LysTyr: 2.65 ± 0.449
0.0LysXaa: 0.0 ± 0.0
Leu
6.418LeuAla: 6.418 ± 0.544
1.001LeuCys: 1.001 ± 0.277
5.358LeuAsp: 5.358 ± 0.576
5.535LeuGlu: 5.535 ± 0.563
2.473LeuPhe: 2.473 ± 0.354
5.064LeuGly: 5.064 ± 0.688
1.06LeuHis: 1.06 ± 0.282
4.828LeuIle: 4.828 ± 0.516
4.239LeuLys: 4.239 ± 0.446
6.359LeuLeu: 6.359 ± 0.734
1.649LeuMet: 1.649 ± 0.291
3.768LeuAsn: 3.768 ± 0.545
2.473LeuPro: 2.473 ± 0.472
2.591LeuGln: 2.591 ± 0.328
5.005LeuArg: 5.005 ± 0.576
6.653LeuSer: 6.653 ± 0.529
5.005LeuThr: 5.005 ± 0.537
4.298LeuVal: 4.298 ± 0.516
0.765LeuTrp: 0.765 ± 0.235
2.767LeuTyr: 2.767 ± 0.34
0.0LeuXaa: 0.0 ± 0.0
Met
2.591MetAla: 2.591 ± 0.377
0.0MetCys: 0.0 ± 0.0
1.472MetAsp: 1.472 ± 0.346
2.355MetGlu: 2.355 ± 0.299
0.824MetPhe: 0.824 ± 0.226
1.766MetGly: 1.766 ± 0.386
0.236MetHis: 0.236 ± 0.135
1.472MetIle: 1.472 ± 0.313
1.825MetLys: 1.825 ± 0.354
1.531MetLeu: 1.531 ± 0.253
0.707MetMet: 0.707 ± 0.214
1.295MetAsn: 1.295 ± 0.357
0.765MetPro: 0.765 ± 0.216
0.707MetGln: 0.707 ± 0.214
1.413MetArg: 1.413 ± 0.309
2.414MetSer: 2.414 ± 0.446
1.707MetThr: 1.707 ± 0.313
1.236MetVal: 1.236 ± 0.255
0.294MetTrp: 0.294 ± 0.122
1.06MetTyr: 1.06 ± 0.288
0.0MetXaa: 0.0 ± 0.0
Asn
3.65AsnAla: 3.65 ± 0.572
0.471AsnCys: 0.471 ± 0.165
2.296AsnAsp: 2.296 ± 0.33
4.239AsnGlu: 4.239 ± 0.537
1.295AsnPhe: 1.295 ± 0.238
3.65AsnGly: 3.65 ± 0.605
0.765AsnHis: 0.765 ± 0.203
2.885AsnIle: 2.885 ± 0.344
2.885AsnLys: 2.885 ± 0.405
4.122AsnLeu: 4.122 ± 0.431
0.942AsnMet: 0.942 ± 0.241
1.825AsnAsn: 1.825 ± 0.386
1.472AsnPro: 1.472 ± 0.265
1.06AsnGln: 1.06 ± 0.223
3.415AsnArg: 3.415 ± 0.404
2.355AsnSer: 2.355 ± 0.384
2.826AsnThr: 2.826 ± 0.367
4.004AsnVal: 4.004 ± 0.56
0.53AsnTrp: 0.53 ± 0.175
1.649AsnTyr: 1.649 ± 0.307
0.0AsnXaa: 0.0 ± 0.0
Pro
1.766ProAla: 1.766 ± 0.318
0.236ProCys: 0.236 ± 0.121
2.002ProAsp: 2.002 ± 0.435
2.767ProGlu: 2.767 ± 0.509
1.001ProPhe: 1.001 ± 0.255
1.825ProGly: 1.825 ± 0.325
0.53ProHis: 0.53 ± 0.143
2.532ProIle: 2.532 ± 0.375
1.943ProLys: 1.943 ± 0.456
2.414ProLeu: 2.414 ± 0.417
0.53ProMet: 0.53 ± 0.174
1.649ProAsn: 1.649 ± 0.327
0.648ProPro: 0.648 ± 0.204
0.883ProGln: 0.883 ± 0.287
1.236ProArg: 1.236 ± 0.305
1.707ProSer: 1.707 ± 0.378
1.884ProThr: 1.884 ± 0.304
1.236ProVal: 1.236 ± 0.333
0.177ProTrp: 0.177 ± 0.088
1.295ProTyr: 1.295 ± 0.3
0.0ProXaa: 0.0 ± 0.0
Gln
2.179GlnAla: 2.179 ± 0.381
0.53GlnCys: 0.53 ± 0.187
1.766GlnAsp: 1.766 ± 0.342
2.179GlnGlu: 2.179 ± 0.415
1.649GlnPhe: 1.649 ± 0.346
1.354GlnGly: 1.354 ± 0.304
0.707GlnHis: 0.707 ± 0.201
2.355GlnIle: 2.355 ± 0.413
1.825GlnLys: 1.825 ± 0.347
2.65GlnLeu: 2.65 ± 0.324
1.472GlnMet: 1.472 ± 0.252
1.119GlnAsn: 1.119 ± 0.273
0.707GlnPro: 0.707 ± 0.198
1.06GlnGln: 1.06 ± 0.231
3.121GlnArg: 3.121 ± 0.446
1.825GlnSer: 1.825 ± 0.338
2.355GlnThr: 2.355 ± 0.471
1.825GlnVal: 1.825 ± 0.325
0.294GlnTrp: 0.294 ± 0.118
1.119GlnTyr: 1.119 ± 0.298
0.0GlnXaa: 0.0 ± 0.0
Arg
4.004ArgAla: 4.004 ± 0.483
0.589ArgCys: 0.589 ± 0.193
4.239ArgAsp: 4.239 ± 0.399
4.475ArgGlu: 4.475 ± 0.639
2.296ArgPhe: 2.296 ± 0.355
3.415ArgGly: 3.415 ± 0.508
1.354ArgHis: 1.354 ± 0.325
5.24ArgIle: 5.24 ± 0.484
5.652ArgLys: 5.652 ± 0.633
6.065ArgLeu: 6.065 ± 0.616
2.002ArgMet: 2.002 ± 0.3
3.592ArgAsn: 3.592 ± 0.506
1.119ArgPro: 1.119 ± 0.32
2.237ArgGln: 2.237 ± 0.41
4.122ArgArg: 4.122 ± 0.646
3.297ArgSer: 3.297 ± 0.463
3.121ArgThr: 3.121 ± 0.456
4.004ArgVal: 4.004 ± 0.496
0.883ArgTrp: 0.883 ± 0.18
2.473ArgTyr: 2.473 ± 0.446
0.0ArgXaa: 0.0 ± 0.0
Ser
3.827SerAla: 3.827 ± 0.521
0.471SerCys: 0.471 ± 0.188
3.827SerAsp: 3.827 ± 0.506
4.946SerGlu: 4.946 ± 0.758
3.768SerPhe: 3.768 ± 0.576
4.534SerGly: 4.534 ± 0.485
0.942SerHis: 0.942 ± 0.224
3.709SerIle: 3.709 ± 0.58
3.533SerLys: 3.533 ± 0.485
3.65SerLeu: 3.65 ± 0.452
1.649SerMet: 1.649 ± 0.385
2.296SerAsn: 2.296 ± 0.483
1.943SerPro: 1.943 ± 0.336
2.237SerGln: 2.237 ± 0.311
3.121SerArg: 3.121 ± 0.489
4.004SerSer: 4.004 ± 0.883
3.062SerThr: 3.062 ± 0.383
4.828SerVal: 4.828 ± 0.804
0.412SerTrp: 0.412 ± 0.127
2.591SerTyr: 2.591 ± 0.385
0.0SerXaa: 0.0 ± 0.0
Thr
4.593ThrAla: 4.593 ± 0.665
0.353ThrCys: 0.353 ± 0.132
3.65ThrAsp: 3.65 ± 0.646
4.651ThrGlu: 4.651 ± 0.666
2.65ThrPhe: 2.65 ± 0.379
4.593ThrGly: 4.593 ± 0.532
0.53ThrHis: 0.53 ± 0.211
3.709ThrIle: 3.709 ± 0.569
3.474ThrLys: 3.474 ± 0.568
4.357ThrLeu: 4.357 ± 0.551
1.119ThrMet: 1.119 ± 0.26
3.179ThrAsn: 3.179 ± 0.382
1.707ThrPro: 1.707 ± 0.364
2.296ThrGln: 2.296 ± 0.476
2.708ThrArg: 2.708 ± 0.324
3.65ThrSer: 3.65 ± 0.462
3.474ThrThr: 3.474 ± 0.56
4.651ThrVal: 4.651 ± 0.66
1.06ThrTrp: 1.06 ± 0.213
2.591ThrTyr: 2.591 ± 0.412
0.0ThrXaa: 0.0 ± 0.0
Val
3.592ValAla: 3.592 ± 0.439
0.353ValCys: 0.353 ± 0.157
6.241ValAsp: 6.241 ± 0.614
5.652ValGlu: 5.652 ± 0.721
2.885ValPhe: 2.885 ± 0.433
4.298ValGly: 4.298 ± 0.57
1.119ValHis: 1.119 ± 0.263
4.357ValIle: 4.357 ± 0.507
6.065ValLys: 6.065 ± 0.723
5.064ValLeu: 5.064 ± 0.659
1.884ValMet: 1.884 ± 0.385
3.945ValAsn: 3.945 ± 0.406
2.179ValPro: 2.179 ± 0.372
1.943ValGln: 1.943 ± 0.35
4.828ValArg: 4.828 ± 0.639
4.651ValSer: 4.651 ± 0.861
4.357ValThr: 4.357 ± 0.718
4.534ValVal: 4.534 ± 0.416
0.765ValTrp: 0.765 ± 0.261
2.767ValTyr: 2.767 ± 0.464
0.0ValXaa: 0.0 ± 0.0
Trp
0.942TrpAla: 0.942 ± 0.221
0.059TrpCys: 0.059 ± 0.067
0.589TrpAsp: 0.589 ± 0.218
0.942TrpGlu: 0.942 ± 0.39
0.53TrpPhe: 0.53 ± 0.183
0.883TrpGly: 0.883 ± 0.245
0.118TrpHis: 0.118 ± 0.088
0.294TrpIle: 0.294 ± 0.098
0.765TrpLys: 0.765 ± 0.269
1.354TrpLeu: 1.354 ± 0.214
0.294TrpMet: 0.294 ± 0.13
0.942TrpAsn: 0.942 ± 0.291
0.0TrpPro: 0.0 ± 0.0
0.471TrpGln: 0.471 ± 0.193
1.178TrpArg: 1.178 ± 0.218
0.765TrpSer: 0.765 ± 0.203
0.471TrpThr: 0.471 ± 0.161
0.648TrpVal: 0.648 ± 0.201
0.059TrpTrp: 0.059 ± 0.056
0.471TrpTyr: 0.471 ± 0.165
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.062TyrAla: 3.062 ± 0.471
0.412TyrCys: 0.412 ± 0.214
3.474TyrAsp: 3.474 ± 0.485
4.18TyrGlu: 4.18 ± 0.619
1.825TyrPhe: 1.825 ± 0.337
1.884TyrGly: 1.884 ± 0.334
0.765TyrHis: 0.765 ± 0.212
2.767TyrIle: 2.767 ± 0.387
2.237TyrLys: 2.237 ± 0.423
3.003TyrLeu: 3.003 ± 0.436
0.765TyrMet: 0.765 ± 0.186
1.354TyrAsn: 1.354 ± 0.284
1.413TyrPro: 1.413 ± 0.322
1.295TyrGln: 1.295 ± 0.305
2.65TyrArg: 2.65 ± 0.423
1.354TyrSer: 1.354 ± 0.291
2.65TyrThr: 2.65 ± 0.454
3.415TyrVal: 3.415 ± 0.437
0.177TyrTrp: 0.177 ± 0.095
2.179TyrTyr: 2.179 ± 0.485
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 80 proteins (16985 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski