Hallerella succinigenes
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2694 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2M9A7Q5|A0A2M9A7Q5_9BACT Thioredoxin reductase (NADPH) OS=Hallerella succinigenes OX=1896222 GN=BGX16_1651 PE=4 SV=1
MM1 pKa = 7.26 NLKK4 pKa = 10.33 LLAGVSAFAFASAFAQDD21 pKa = 4.32 DD22 pKa = 5.02 YY23 pKa = 11.37 YY24 pKa = 11.72 AEE26 pKa = 5.32 DD27 pKa = 4.08 PAQEE31 pKa = 4.02 ATPVAEE37 pKa = 4.92 APAAEE42 pKa = 4.73 PAPSPYY48 pKa = 8.89 EE49 pKa = 4.11 TEE51 pKa = 4.16 PVAQAPAAVAPATVAPAQNSSSLLAQLDD79 pKa = 3.86 VLRR82 pKa = 11.84 GNAYY86 pKa = 10.31 NFVGSEE92 pKa = 3.9 LAATTVADD100 pKa = 3.82 QLDD103 pKa = 4.55 YY104 pKa = 10.79 PYY106 pKa = 11.04 MMALGQFLYY115 pKa = 10.97 VEE117 pKa = 4.38 PTNALGYY124 pKa = 9.67 VAFNKK129 pKa = 10.53 GLTFYY134 pKa = 11.11 GGLDD138 pKa = 3.36 NSSADD143 pKa = 3.26 VGLFTAGIATAGFGLGLDD161 pKa = 3.37 IGLSKK166 pKa = 11.08 SFVSDD171 pKa = 3.4 GDD173 pKa = 3.9 DD174 pKa = 3.61 DD175 pKa = 4.14 ATVVNAGDD183 pKa = 3.82 DD184 pKa = 3.87 LGVTFAMPLGAYY196 pKa = 9.65 ALSLEE201 pKa = 4.64 AGWLTIDD208 pKa = 5.72 DD209 pKa = 4.44 EE210 pKa = 4.99 VSTDD214 pKa = 3.75 NVEE217 pKa = 3.64 NDD219 pKa = 3.09 YY220 pKa = 11.29 WEE222 pKa = 4.7 LSLIARR228 pKa = 11.84 FTNSPSATTLGWTAGALLLRR248 pKa = 11.84 HH249 pKa = 6.47 AEE251 pKa = 4.21 TTEE254 pKa = 3.62 MTMAGQSSTSASGNSRR270 pKa = 11.84 VEE272 pKa = 3.21 IDD274 pKa = 3.88 PYY276 pKa = 11.31 FNLAAQILGNDD287 pKa = 4.0 MARR290 pKa = 11.84 VMIGSNNTLGIQIYY304 pKa = 10.39 DD305 pKa = 3.85 GGDD308 pKa = 3.05 AGIKK312 pKa = 9.6 SHH314 pKa = 6.45 TEE316 pKa = 3.26 FGLQLEE322 pKa = 4.42 PNIWADD328 pKa = 3.36 FALNEE333 pKa = 4.02 NFLFFGGVSHH343 pKa = 6.96 NVLLFGYY350 pKa = 9.84 EE351 pKa = 3.98 SVKK354 pKa = 10.9 YY355 pKa = 10.83 VDD357 pKa = 3.91 GDD359 pKa = 3.92 KK360 pKa = 10.77 ISAIQLHH367 pKa = 5.24 TEE369 pKa = 3.85 RR370 pKa = 11.84 TRR372 pKa = 11.84 AQLGLRR378 pKa = 11.84 AQYY381 pKa = 11.55 KK382 pKa = 8.41 MVAVEE387 pKa = 4.5 ASLTNEE393 pKa = 4.16 VYY395 pKa = 10.0 TKK397 pKa = 10.22 TVAAIFNGDD406 pKa = 4.18 NIVADD411 pKa = 4.64 LGIFLNFF418 pKa = 3.64
Molecular weight: 44.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.757
IPC2_protein 3.91
IPC_protein 3.91
Toseland 3.706
ProMoST 4.062
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.808
Rodwell 3.745
Grimsley 3.617
Solomon 3.884
Lehninger 3.846
Nozaki 3.999
DTASelect 4.228
Thurlkill 3.745
EMBOSS 3.821
Sillero 4.037
Patrickios 1.901
IPC_peptide 3.884
IPC2_peptide 4.012
IPC2.peptide.svr19 3.909
Protein with the highest isoelectric point:
>tr|A0A2M9AAU5|A0A2M9AAU5_9BACT Motility quorum-sensing regulator/GCU-specific mRNA interferase toxin OS=Hallerella succinigenes OX=1896222 GN=BGX16_2781 PE=4 SV=1
MM1 pKa = 7.51 GAPLRR6 pKa = 11.84 RR7 pKa = 11.84 GLLCFVRR14 pKa = 11.84 HH15 pKa = 5.36 IKK17 pKa = 9.74 MYY19 pKa = 9.55 FCIHH23 pKa = 5.16 FRR25 pKa = 11.84 IRR27 pKa = 11.84 IHH29 pKa = 6.42 QHH31 pKa = 4.8 FQPCFSTLRR40 pKa = 11.84 FLLKK44 pKa = 10.14 KK45 pKa = 9.36 QHH47 pKa = 6.21 FPFGTGFAYY56 pKa = 10.36 SCMFEE61 pKa = 4.74 KK62 pKa = 10.55 ISDD65 pKa = 3.83 NKK67 pKa = 10.46 PLTKK71 pKa = 10.24 EE72 pKa = 3.74 KK73 pKa = 10.67 SLCSHH78 pKa = 6.52 YY79 pKa = 10.76 NSSSPASPPSTTSSNTSTATNNPPHH104 pKa = 7.09 PNQKK108 pKa = 9.58 KK109 pKa = 8.3 PRR111 pKa = 11.84 YY112 pKa = 7.32 MRR114 pKa = 11.84 NRR116 pKa = 11.84 TRR118 pKa = 11.84 IPRR121 pKa = 11.84 QILLNPQLSYY131 pKa = 11.14 LPPEE135 pKa = 4.46 TNPGATRR142 pKa = 11.84 IGDD145 pKa = 3.87 FSLFRR150 pKa = 11.84 VKK152 pKa = 10.79 LCDD155 pKa = 4.23 FSRR158 pKa = 11.84 KK159 pKa = 8.78
Molecular weight: 18.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.343
IPC2_protein 9.765
IPC_protein 10.335
Toseland 10.657
ProMoST 10.335
Dawson 10.76
Bjellqvist 10.452
Wikipedia 10.935
Rodwell 11.125
Grimsley 10.818
Solomon 10.833
Lehninger 10.818
Nozaki 10.672
DTASelect 10.438
Thurlkill 10.657
EMBOSS 11.052
Sillero 10.701
Patrickios 10.847
IPC_peptide 10.847
IPC2_peptide 9.604
IPC2.peptide.svr19 8.499
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2694
0
2694
925557
29
3890
343.6
38.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.316 ± 0.046
1.249 ± 0.018
5.724 ± 0.035
6.735 ± 0.049
4.853 ± 0.035
6.815 ± 0.05
1.833 ± 0.019
6.043 ± 0.041
6.771 ± 0.048
9.249 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.447 ± 0.021
4.417 ± 0.031
3.92 ± 0.027
3.075 ± 0.025
4.731 ± 0.037
6.975 ± 0.053
5.243 ± 0.045
6.748 ± 0.039
1.251 ± 0.017
3.604 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here