Lederbergia lentus (Bacillus lentus)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Lederbergia

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4072 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2X4WN03|A0A2X4WN03_LEDLE Alkylhydroperoxidase AhpD core OS=Lederbergia lentus OX=1467 GN=NCTC4824_02573 PE=4 SV=1
MM1 pKa = 7.53SIIKK5 pKa = 10.17GAILSVGLAFSFILVGCQNNGSSEE29 pKa = 4.21TTNDD33 pKa = 3.34DD34 pKa = 3.44SEE36 pKa = 4.65TRR38 pKa = 11.84LSSGNQDD45 pKa = 2.71STLPIEE51 pKa = 4.35DD52 pKa = 3.75TEE54 pKa = 4.37YY55 pKa = 11.46GRR57 pKa = 11.84GAQSDD62 pKa = 4.4GVDD65 pKa = 3.41TNAKK69 pKa = 9.73EE70 pKa = 4.03EE71 pKa = 4.08PTRR74 pKa = 11.84IISDD78 pKa = 3.76DD79 pKa = 3.67ANSIIIYY86 pKa = 8.72FSRR89 pKa = 11.84SGNTQNLARR98 pKa = 11.84MIYY101 pKa = 10.15NEE103 pKa = 4.17IGGDD107 pKa = 3.64MLEE110 pKa = 4.22LTVTNPYY117 pKa = 10.16PSDD120 pKa = 3.39YY121 pKa = 10.52SQAVEE126 pKa = 4.01RR127 pKa = 11.84ADD129 pKa = 3.6EE130 pKa = 4.1EE131 pKa = 4.54RR132 pKa = 11.84DD133 pKa = 3.51NQEE136 pKa = 3.78YY137 pKa = 10.61PEE139 pKa = 5.78IDD141 pKa = 3.14TDD143 pKa = 4.99IPDD146 pKa = 4.08LSQYY150 pKa = 9.58DD151 pKa = 4.16TVYY154 pKa = 10.86LGYY157 pKa = 8.22QTWSMTLSNPMISFLMDD174 pKa = 3.44HH175 pKa = 7.02GSNFSGKK182 pKa = 8.46TIYY185 pKa = 10.27PFSTNAGYY193 pKa = 11.2GEE195 pKa = 4.94GDD197 pKa = 3.35TIEE200 pKa = 5.0RR201 pKa = 11.84IEE203 pKa = 4.48EE204 pKa = 3.96FAPDD208 pKa = 3.46ASVATSFSIQDD219 pKa = 3.4EE220 pKa = 4.38DD221 pKa = 5.1LLDD224 pKa = 3.91NQEE227 pKa = 5.2QVTSWLNEE235 pKa = 3.77NN236 pKa = 3.95

Molecular weight: 26.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2X4ZGL1|A0A2X4ZGL1_LEDLE Methyltransferase OS=Lederbergia lentus OX=1467 GN=NCTC4824_02421 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.59RR3 pKa = 11.84TFQPNKK9 pKa = 8.24RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84SKK14 pKa = 9.83VHH16 pKa = 5.77GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSKK25 pKa = 9.28TGRR28 pKa = 11.84NILAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.84GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4072

0

4072

1216744

29

2438

298.8

33.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.814 ± 0.035

0.683 ± 0.011

5.153 ± 0.03

7.453 ± 0.041

4.673 ± 0.036

6.772 ± 0.034

2.053 ± 0.016

8.475 ± 0.04

6.932 ± 0.029

9.72 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.918 ± 0.017

4.654 ± 0.03

3.512 ± 0.02

3.65 ± 0.027

3.815 ± 0.025

6.001 ± 0.031

5.405 ± 0.028

6.643 ± 0.034

1.105 ± 0.016

3.569 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski