Oidium neolycopersici
Average proteome isoelectric point is 6.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6847 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A420HBL8|A0A420HBL8_9PEZI 3'(2') 5'-bisphosphate nucleotidase OS=Oidium neolycopersici OX=212602 GN=OnM2_094026 PE=3 SV=1
MM1 pKa = 7.56 KK2 pKa = 10.54 GLFAPLVAAVVLVDD16 pKa = 4.74 ALTHH20 pKa = 6.0 HH21 pKa = 7.32 PIVYY25 pKa = 9.71 YY26 pKa = 10.21 PKK28 pKa = 9.1 EE29 pKa = 3.85 TDD31 pKa = 3.15 AASLNNEE38 pKa = 4.17 PGNVLKK44 pKa = 10.95 SDD46 pKa = 3.43 VVYY49 pKa = 11.24 QNVPTNNQDD58 pKa = 3.4 VEE60 pKa = 4.31 PASAEE65 pKa = 3.84 AVASKK70 pKa = 11.19 NNISDD75 pKa = 3.51 QPDD78 pKa = 3.61 DD79 pKa = 4.39 VDD81 pKa = 4.58 EE82 pKa = 5.18 LDD84 pKa = 4.42 SNDD87 pKa = 3.82 EE88 pKa = 4.05 EE89 pKa = 6.5 AEE91 pKa = 4.29 TKK93 pKa = 10.43 PNPTEE98 pKa = 5.19 FIDD101 pKa = 5.2 DD102 pKa = 3.9 NQTSKK107 pKa = 9.98 LTPPDD112 pKa = 3.85 VKK114 pKa = 10.39 PDD116 pKa = 4.34 SIDD119 pKa = 4.15 DD120 pKa = 4.21 DD121 pKa = 4.39 NLNKK125 pKa = 9.92 PAGIDD130 pKa = 3.75 FPDD133 pKa = 4.46 GEE135 pKa = 4.25 PAEE138 pKa = 4.36 EE139 pKa = 4.5 KK140 pKa = 10.22 FDD142 pKa = 4.0 SVEE145 pKa = 3.96 IPNTTLYY152 pKa = 7.23 TTSTVYY158 pKa = 9.74 STVTSTVTDD167 pKa = 3.72 CVTTVTDD174 pKa = 3.86 CPEE177 pKa = 4.03 IGSVVTKK184 pKa = 9.33 TISMYY189 pKa = 8.68 TTVCPVTTPTSVAPSSDD206 pKa = 3.04 EE207 pKa = 3.94 LTNEE211 pKa = 4.94 DD212 pKa = 3.88 SDD214 pKa = 4.92 SPNSDD219 pKa = 2.15 ISEE222 pKa = 4.12 SDD224 pKa = 3.32 EE225 pKa = 4.36 PEE227 pKa = 3.77 SDD229 pKa = 3.44 KK230 pKa = 11.54 PEE232 pKa = 4.22 SDD234 pKa = 3.52 EE235 pKa = 4.6 PEE237 pKa = 3.9 SDD239 pKa = 3.59 EE240 pKa = 4.75 PEE242 pKa = 4.33 SEE244 pKa = 4.52 SEE246 pKa = 4.45 SDD248 pKa = 3.61 MEE250 pKa = 5.46 PDD252 pKa = 3.89 TEE254 pKa = 4.92 SLDD257 pKa = 3.69 IPDD260 pKa = 4.58 APEE263 pKa = 3.94 PTQAPYY269 pKa = 8.5 YY270 pKa = 8.82 TKK272 pKa = 10.35 TIEE275 pKa = 4.0 TTVWYY280 pKa = 8.08 TVTDD284 pKa = 3.98 CTPKK288 pKa = 9.94 EE289 pKa = 4.19 TDD291 pKa = 3.66 CPIGQEE297 pKa = 3.96 TSSIHH302 pKa = 5.41 TRR304 pKa = 11.84 TEE306 pKa = 3.76 IYY308 pKa = 9.96 PVTDD312 pKa = 3.59 IPHH315 pKa = 6.24 FWSPNSTQTQPYY327 pKa = 8.16 ATGSPSSYY335 pKa = 10.34 PEE337 pKa = 3.74 QPSNDD342 pKa = 3.14 NVTIPTTPPNYY353 pKa = 9.64 TINAGSAQKK362 pKa = 11.1 ANALLMSFGLVAASAVAIFQGLII385 pKa = 3.31
Molecular weight: 41.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.723
IPC2_protein 3.63
IPC_protein 3.643
Toseland 3.427
ProMoST 3.795
Dawson 3.63
Bjellqvist 3.783
Wikipedia 3.554
Rodwell 3.465
Grimsley 3.338
Solomon 3.617
Lehninger 3.579
Nozaki 3.745
DTASelect 3.961
Thurlkill 3.478
EMBOSS 3.554
Sillero 3.757
Patrickios 0.985
IPC_peptide 3.617
IPC2_peptide 3.745
IPC2.peptide.svr19 3.72
Protein with the highest isoelectric point:
>tr|A0A420HKP3|A0A420HKP3_9PEZI Putative effector protein OS=Oidium neolycopersici OX=212602 GN=OnM2_070029 PE=4 SV=1
MM1 pKa = 7.24 TALLLCTSLVMLCLRR16 pKa = 11.84 SRR18 pKa = 11.84 SSCGGSLSRR27 pKa = 11.84 IYY29 pKa = 10.74 SRR31 pKa = 11.84 EE32 pKa = 3.61 CLARR36 pKa = 11.84 SRR38 pKa = 11.84 AVWHH42 pKa = 6.53 ILTARR47 pKa = 11.84 DD48 pKa = 3.64 HH49 pKa = 6.98 NITATIVLEE58 pKa = 3.78 QRR60 pKa = 11.84 KK61 pKa = 8.84 RR62 pKa = 11.84 RR63 pKa = 11.84 KK64 pKa = 10.06 SCDD67 pKa = 2.63 AYY69 pKa = 10.65 APKK72 pKa = 10.47 KK73 pKa = 10.05 ILQQILEE80 pKa = 4.18 GPRR83 pKa = 11.84 RR84 pKa = 11.84 YY85 pKa = 10.23 RR86 pKa = 11.84 MPLGFTNKK94 pKa = 9.67 RR95 pKa = 11.84 QPEE98 pKa = 4.08 PQISPVLHH106 pKa = 6.51 EE107 pKa = 5.2 FIQQ110 pKa = 3.79
Molecular weight: 12.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.354
IPC2_protein 9.589
IPC_protein 10.394
Toseland 10.57
ProMoST 10.277
Dawson 10.687
Bjellqvist 10.423
Wikipedia 10.891
Rodwell 10.847
Grimsley 10.73
Solomon 10.789
Lehninger 10.76
Nozaki 10.613
DTASelect 10.394
Thurlkill 10.584
EMBOSS 10.965
Sillero 10.628
Patrickios 10.599
IPC_peptide 10.789
IPC2_peptide 9.794
IPC2.peptide.svr19 8.472
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6847
0
6847
3086985
31
4886
450.9
50.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.255 ± 0.022
1.317 ± 0.01
5.43 ± 0.019
6.522 ± 0.031
3.914 ± 0.02
5.471 ± 0.027
2.244 ± 0.011
6.666 ± 0.022
6.487 ± 0.031
9.264 ± 0.031
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.002 ± 0.009
5.224 ± 0.02
4.973 ± 0.022
4.098 ± 0.021
5.616 ± 0.02
9.369 ± 0.033
5.745 ± 0.015
5.299 ± 0.021
1.201 ± 0.011
2.901 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here