Oidium neolycopersici

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Erysiphales; Erysiphaceae; Oidium

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6847 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A420HBL8|A0A420HBL8_9PEZI 3'(2') 5'-bisphosphate nucleotidase OS=Oidium neolycopersici OX=212602 GN=OnM2_094026 PE=3 SV=1
MM1 pKa = 7.56KK2 pKa = 10.54GLFAPLVAAVVLVDD16 pKa = 4.74ALTHH20 pKa = 6.0HH21 pKa = 7.32PIVYY25 pKa = 9.71YY26 pKa = 10.21PKK28 pKa = 9.1EE29 pKa = 3.85TDD31 pKa = 3.15AASLNNEE38 pKa = 4.17PGNVLKK44 pKa = 10.95SDD46 pKa = 3.43VVYY49 pKa = 11.24QNVPTNNQDD58 pKa = 3.4VEE60 pKa = 4.31PASAEE65 pKa = 3.84AVASKK70 pKa = 11.19NNISDD75 pKa = 3.51QPDD78 pKa = 3.61DD79 pKa = 4.39VDD81 pKa = 4.58EE82 pKa = 5.18LDD84 pKa = 4.42SNDD87 pKa = 3.82EE88 pKa = 4.05EE89 pKa = 6.5AEE91 pKa = 4.29TKK93 pKa = 10.43PNPTEE98 pKa = 5.19FIDD101 pKa = 5.2DD102 pKa = 3.9NQTSKK107 pKa = 9.98LTPPDD112 pKa = 3.85VKK114 pKa = 10.39PDD116 pKa = 4.34SIDD119 pKa = 4.15DD120 pKa = 4.21DD121 pKa = 4.39NLNKK125 pKa = 9.92PAGIDD130 pKa = 3.75FPDD133 pKa = 4.46GEE135 pKa = 4.25PAEE138 pKa = 4.36EE139 pKa = 4.5KK140 pKa = 10.22FDD142 pKa = 4.0SVEE145 pKa = 3.96IPNTTLYY152 pKa = 7.23TTSTVYY158 pKa = 9.74STVTSTVTDD167 pKa = 3.72CVTTVTDD174 pKa = 3.86CPEE177 pKa = 4.03IGSVVTKK184 pKa = 9.33TISMYY189 pKa = 8.68TTVCPVTTPTSVAPSSDD206 pKa = 3.04EE207 pKa = 3.94LTNEE211 pKa = 4.94DD212 pKa = 3.88SDD214 pKa = 4.92SPNSDD219 pKa = 2.15ISEE222 pKa = 4.12SDD224 pKa = 3.32EE225 pKa = 4.36PEE227 pKa = 3.77SDD229 pKa = 3.44KK230 pKa = 11.54PEE232 pKa = 4.22SDD234 pKa = 3.52EE235 pKa = 4.6PEE237 pKa = 3.9SDD239 pKa = 3.59EE240 pKa = 4.75PEE242 pKa = 4.33SEE244 pKa = 4.52SEE246 pKa = 4.45SDD248 pKa = 3.61MEE250 pKa = 5.46PDD252 pKa = 3.89TEE254 pKa = 4.92SLDD257 pKa = 3.69IPDD260 pKa = 4.58APEE263 pKa = 3.94PTQAPYY269 pKa = 8.5YY270 pKa = 8.82TKK272 pKa = 10.35TIEE275 pKa = 4.0TTVWYY280 pKa = 8.08TVTDD284 pKa = 3.98CTPKK288 pKa = 9.94EE289 pKa = 4.19TDD291 pKa = 3.66CPIGQEE297 pKa = 3.96TSSIHH302 pKa = 5.41TRR304 pKa = 11.84TEE306 pKa = 3.76IYY308 pKa = 9.96PVTDD312 pKa = 3.59IPHH315 pKa = 6.24FWSPNSTQTQPYY327 pKa = 8.16ATGSPSSYY335 pKa = 10.34PEE337 pKa = 3.74QPSNDD342 pKa = 3.14NVTIPTTPPNYY353 pKa = 9.64TINAGSAQKK362 pKa = 11.1ANALLMSFGLVAASAVAIFQGLII385 pKa = 3.31

Molecular weight:
41.46 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A420HKP3|A0A420HKP3_9PEZI Putative effector protein OS=Oidium neolycopersici OX=212602 GN=OnM2_070029 PE=4 SV=1
MM1 pKa = 7.24TALLLCTSLVMLCLRR16 pKa = 11.84SRR18 pKa = 11.84SSCGGSLSRR27 pKa = 11.84IYY29 pKa = 10.74SRR31 pKa = 11.84EE32 pKa = 3.61CLARR36 pKa = 11.84SRR38 pKa = 11.84AVWHH42 pKa = 6.53ILTARR47 pKa = 11.84DD48 pKa = 3.64HH49 pKa = 6.98NITATIVLEE58 pKa = 3.78QRR60 pKa = 11.84KK61 pKa = 8.84RR62 pKa = 11.84RR63 pKa = 11.84KK64 pKa = 10.06SCDD67 pKa = 2.63AYY69 pKa = 10.65APKK72 pKa = 10.47KK73 pKa = 10.05ILQQILEE80 pKa = 4.18GPRR83 pKa = 11.84RR84 pKa = 11.84YY85 pKa = 10.23RR86 pKa = 11.84MPLGFTNKK94 pKa = 9.67RR95 pKa = 11.84QPEE98 pKa = 4.08PQISPVLHH106 pKa = 6.51EE107 pKa = 5.2FIQQ110 pKa = 3.79

Molecular weight:
12.68 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6847

0

6847

3086985

31

4886

450.9

50.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.255 ± 0.022

1.317 ± 0.01

5.43 ± 0.019

6.522 ± 0.031

3.914 ± 0.02

5.471 ± 0.027

2.244 ± 0.011

6.666 ± 0.022

6.487 ± 0.031

9.264 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.002 ± 0.009

5.224 ± 0.02

4.973 ± 0.022

4.098 ± 0.021

5.616 ± 0.02

9.369 ± 0.033

5.745 ± 0.015

5.299 ± 0.021

1.201 ± 0.011

2.901 ± 0.016

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski