Polar bear mastadenovirus 1
Average proteome isoelectric point is 6.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 21 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z4QJ25|A0A2Z4QJ25_9ADEN PVI OS=Polar bear mastadenovirus 1 OX=2250215 PE=4 SV=1
MM1 pKa = 7.9 PLFSLRR7 pKa = 11.84 SPRR10 pKa = 11.84 CSRR13 pKa = 11.84 SAFRR17 pKa = 11.84 GGPSCLSFDD26 pKa = 4.07 FPIVMRR32 pKa = 11.84 YY33 pKa = 7.7 LTVPLDD39 pKa = 3.38 YY40 pKa = 11.74 AMIRR44 pKa = 11.84 IIDD47 pKa = 4.28 EE48 pKa = 3.83 VDD50 pKa = 2.87 MDD52 pKa = 4.8 DD53 pKa = 5.67 GVDD56 pKa = 3.82 AVDD59 pKa = 4.9 DD60 pKa = 3.82 SWDD63 pKa = 3.28 VSLYY67 pKa = 11.1 DD68 pKa = 4.88 LFDD71 pKa = 4.02 ISPEE75 pKa = 3.9 PQPAPIEE82 pKa = 4.19 EE83 pKa = 4.71 GEE85 pKa = 4.33 PEE87 pKa = 4.38 VLRR90 pKa = 11.84 TPSISPVPSPAILTPPLTPEE110 pKa = 4.47 SVPEE114 pKa = 4.01 MDD116 pKa = 4.07 LSCYY120 pKa = 10.5 EE121 pKa = 4.38 DD122 pKa = 3.94 MPPSSEE128 pKa = 4.76 DD129 pKa = 3.21 EE130 pKa = 4.32 SEE132 pKa = 4.82 PIVLDD137 pKa = 3.67 YY138 pKa = 11.4 PEE140 pKa = 5.42 VPGQQCSSCNQFRR153 pKa = 11.84 DD154 pKa = 3.7 RR155 pKa = 11.84 LGDD158 pKa = 3.72 PYY160 pKa = 11.1 VLCSLCYY167 pKa = 8.77 MRR169 pKa = 11.84 RR170 pKa = 11.84 ISTFVYY176 pKa = 9.39 SKK178 pKa = 10.83 YY179 pKa = 10.75 GAGII183 pKa = 3.72
Molecular weight: 20.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.781
IPC2_protein 3.897
IPC_protein 3.884
Toseland 3.668
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.795
Rodwell 3.706
Grimsley 3.579
Solomon 3.859
Lehninger 3.821
Nozaki 3.986
DTASelect 4.202
Thurlkill 3.719
EMBOSS 3.808
Sillero 3.999
Patrickios 0.629
IPC_peptide 3.859
IPC2_peptide 3.973
IPC2.peptide.svr19 3.897
Protein with the highest isoelectric point:
>tr|A0A2Z4QJE3|A0A2Z4QJE3_9ADEN PIIIa protein OS=Polar bear mastadenovirus 1 OX=2250215 PE=3 SV=1
MM1 pKa = 7.9 DD2 pKa = 3.83 VFYY5 pKa = 10.73 IAVDD9 pKa = 3.61 EE10 pKa = 4.46 KK11 pKa = 11.74 DD12 pKa = 3.23 MSKK15 pKa = 8.77 VTFRR19 pKa = 11.84 VRR21 pKa = 11.84 VPVVNKK27 pKa = 7.37 TEE29 pKa = 3.88 RR30 pKa = 11.84 GLRR33 pKa = 11.84 RR34 pKa = 11.84 KK35 pKa = 9.83 RR36 pKa = 11.84 RR37 pKa = 11.84 TKK39 pKa = 10.21 KK40 pKa = 10.18 LQGGFLPLLAPIIAAAIGAIPGIASVALAASQKK73 pKa = 10.36 HH74 pKa = 4.71
Molecular weight: 8.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.283
IPC2_protein 9.897
IPC_protein 10.833
Toseland 11.228
ProMoST 11.184
Dawson 11.272
Bjellqvist 11.038
Wikipedia 11.535
Rodwell 11.506
Grimsley 11.286
Solomon 11.52
Lehninger 11.462
Nozaki 11.213
DTASelect 11.038
Thurlkill 11.213
EMBOSS 11.652
Sillero 11.213
Patrickios 11.257
IPC_peptide 11.52
IPC2_peptide 10.014
IPC2.peptide.svr19 8.709
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
20
1
21
8008
74
913
381.3
43.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.857 ± 0.199
1.711 ± 0.319
5.519 ± 0.283
5.832 ± 0.428
4.608 ± 0.248
5.644 ± 0.442
2.485 ± 0.187
5.644 ± 0.318
4.92 ± 0.538
9.366 ± 0.453
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.473 ± 0.293
5.42 ± 0.324
6.056 ± 0.411
3.709 ± 0.292
6.406 ± 0.46
7.493 ± 0.354
5.869 ± 0.334
5.769 ± 0.367
1.324 ± 0.148
3.896 ± 0.34
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here