Rhodobacteraceae bacterium KLH11

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; unclassified Rhodobacteraceae

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4229 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B9NLX0|B9NLX0_9RHOB Uncharacterized protein OS=Rhodobacteraceae bacterium KLH11 OX=467661 GN=RKLH11_2143 PE=4 SV=1
MM1 pKa = 7.7IFDD4 pKa = 4.05VDD6 pKa = 4.0PEE8 pKa = 4.24FSNSEE13 pKa = 3.61EE14 pKa = 4.25WYY16 pKa = 10.0QAIPEE21 pKa = 4.2DD22 pKa = 4.36SRR24 pKa = 11.84PLKK27 pKa = 10.28DD28 pKa = 3.22QPFYY32 pKa = 11.33HH33 pKa = 7.11LLAEE37 pKa = 4.59NDD39 pKa = 3.15QSFYY43 pKa = 10.49VAYY46 pKa = 10.55VSEE49 pKa = 4.27QNLVEE54 pKa = 4.46DD55 pKa = 3.91QSGEE59 pKa = 4.19PVEE62 pKa = 5.56HH63 pKa = 7.26PDD65 pKa = 3.13IPNMFGPFQDD75 pKa = 3.4GSYY78 pKa = 10.29PLHH81 pKa = 6.17FQLNN85 pKa = 3.9

Molecular weight: 9.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B9NLC0|B9NLC0_9RHOB Binding-protein-dependent transport systems inner membrane component OS=Rhodobacteraceae bacterium KLH11 OX=467661 GN=RKLH11_836 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4229

0

4229

1272411

29

12584

300.9

32.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.276 ± 0.056

0.919 ± 0.016

6.293 ± 0.053

5.964 ± 0.039

3.819 ± 0.025

8.583 ± 0.078

2.089 ± 0.02

5.437 ± 0.023

3.43 ± 0.034

9.793 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.676 ± 0.034

3.004 ± 0.033

4.813 ± 0.037

3.638 ± 0.024

6.313 ± 0.042

5.52 ± 0.048

5.599 ± 0.042

7.141 ± 0.034

1.352 ± 0.019

2.33 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski