Amino acid dipepetide frequency for Rhodobacteraceae bacterium KLH11

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.779AlaAla: 13.779 ± 0.196	1.068AlaCys: 1.068 ± 0.033	6.77AlaAsp: 6.77 ± 0.104	7.659AlaGlu: 7.659 ± 0.127	4.053AlaPhe: 4.053 ± 0.066	9.462AlaGly: 9.462 ± 0.124	2.303AlaHis: 2.303 ± 0.05	5.758AlaIle: 5.758 ± 0.076	3.801AlaLys: 3.801 ± 0.075	12.209AlaLeu: 12.209 ± 0.163	3.353AlaMet: 3.353 ± 0.07	2.987AlaAsn: 2.987 ± 0.055	4.886AlaPro: 4.886 ± 0.071	4.818AlaGln: 4.818 ± 0.066	7.571AlaArg: 7.571 ± 0.105	5.53AlaSer: 5.53 ± 0.089	5.628AlaThr: 5.628 ± 0.08	7.467AlaVal: 7.467 ± 0.084	1.184AlaTrp: 1.184 ± 0.035	2.474AlaTyr: 2.474 ± 0.042	0.002AlaXaa: 0.002 ± 0.001
Cys	1.065CysAla: 1.065 ± 0.037	0.127CysCys: 0.127 ± 0.01	0.655CysAsp: 0.655 ± 0.027	0.484CysGlu: 0.484 ± 0.02	0.361CysPhe: 0.361 ± 0.016	0.917CysGly: 0.917 ± 0.035	0.262CysHis: 0.262 ± 0.016	0.457CysIle: 0.457 ± 0.02	0.268CysLys: 0.268 ± 0.014	0.846CysLeu: 0.846 ± 0.027	0.182CysMet: 0.182 ± 0.012	0.263CysAsn: 0.263 ± 0.014	0.5CysPro: 0.5 ± 0.022	0.263CysGln: 0.263 ± 0.015	0.555CysArg: 0.555 ± 0.022	0.523CysSer: 0.523 ± 0.021	0.482CysThr: 0.482 ± 0.019	0.626CysVal: 0.626 ± 0.025	0.133CysTrp: 0.133 ± 0.01	0.224CysTyr: 0.224 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	6.848AspAla: 6.848 ± 0.088	0.571AspCys: 0.571 ± 0.021	3.623AspAsp: 3.623 ± 0.087	3.739AspGlu: 3.739 ± 0.062	2.427AspPhe: 2.427 ± 0.048	5.883AspGly: 5.883 ± 0.18	1.507AspHis: 1.507 ± 0.039	3.566AspIle: 3.566 ± 0.065	1.962AspLys: 1.962 ± 0.041	6.654AspLeu: 6.654 ± 0.101	1.633AspMet: 1.633 ± 0.041	1.582AspAsn: 1.582 ± 0.047	3.476AspPro: 3.476 ± 0.054	2.438AspGln: 2.438 ± 0.046	4.078AspArg: 4.078 ± 0.053	2.382AspSer: 2.382 ± 0.058	3.321AspThr: 3.321 ± 0.111	4.501AspVal: 4.501 ± 0.072	1.116AspTrp: 1.116 ± 0.033	1.619AspTyr: 1.619 ± 0.039	0.001AspXaa: 0.001 ± 0.001
Glu	7.381GluAla: 7.381 ± 0.125	0.403GluCys: 0.403 ± 0.021	3.482GluAsp: 3.482 ± 0.062	3.406GluGlu: 3.406 ± 0.071	2.08GluPhe: 2.08 ± 0.039	4.585GluGly: 4.585 ± 0.072	1.224GluHis: 1.224 ± 0.03	3.751GluIle: 3.751 ± 0.057	2.299GluLys: 2.299 ± 0.049	5.56GluLeu: 5.56 ± 0.072	1.906GluMet: 1.906 ± 0.042	2.105GluAsn: 2.105 ± 0.049	2.526GluPro: 2.526 ± 0.054	2.375GluGln: 2.375 ± 0.05	3.994GluArg: 3.994 ± 0.064	2.318GluSer: 2.318 ± 0.041	3.944GluThr: 3.944 ± 0.063	4.311GluVal: 4.311 ± 0.068	0.754GluTrp: 0.754 ± 0.026	1.239GluTyr: 1.239 ± 0.034	0.001GluXaa: 0.001 ± 0.001
Phe	4.213PheAla: 4.213 ± 0.061	0.464PheCys: 0.464 ± 0.02	3.043PheAsp: 3.043 ± 0.061	2.426PheGlu: 2.426 ± 0.041	1.464PhePhe: 1.464 ± 0.04	3.751PheGly: 3.751 ± 0.068	0.769PheHis: 0.769 ± 0.028	1.769PheIle: 1.769 ± 0.043	1.025PheLys: 1.025 ± 0.028	3.456PheLeu: 3.456 ± 0.068	0.904PheMet: 0.904 ± 0.032	1.219PheAsn: 1.219 ± 0.035	1.511PhePro: 1.511 ± 0.041	1.213PheGln: 1.213 ± 0.032	2.126PheArg: 2.126 ± 0.047	2.318PheSer: 2.318 ± 0.057	2.116PheThr: 2.116 ± 0.04	2.777PheVal: 2.777 ± 0.049	0.602PheTrp: 0.602 ± 0.024	1.018PheTyr: 1.018 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	8.977GlyAla: 8.977 ± 0.118	0.861GlyCys: 0.861 ± 0.032	5.243GlyAsp: 5.243 ± 0.177	4.651GlyGlu: 4.651 ± 0.07	3.761GlyPhe: 3.761 ± 0.063	7.63GlyGly: 7.63 ± 0.402	1.875GlyHis: 1.875 ± 0.045	4.577GlyIle: 4.577 ± 0.076	3.391GlyLys: 3.391 ± 0.054	8.687GlyLeu: 8.687 ± 0.102	2.393GlyMet: 2.393 ± 0.051	2.57GlyAsn: 2.57 ± 0.106	3.414GlyPro: 3.414 ± 0.061	3.441GlyGln: 3.441 ± 0.058	5.016GlyArg: 5.016 ± 0.059	4.547GlySer: 4.547 ± 0.125	4.756GlyThr: 4.756 ± 0.101	6.223GlyVal: 6.223 ± 0.085	1.42GlyTrp: 1.42 ± 0.038	2.396GlyTyr: 2.396 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	2.107HisAla: 2.107 ± 0.047	0.253HisCys: 0.253 ± 0.015	1.295HisAsp: 1.295 ± 0.036	1.085HisGlu: 1.085 ± 0.027	0.859HisPhe: 0.859 ± 0.025	1.833HisGly: 1.833 ± 0.046	0.582HisHis: 0.582 ± 0.026	1.109HisIle: 1.109 ± 0.034	0.648HisLys: 0.648 ± 0.021	2.143HisLeu: 2.143 ± 0.047	0.593HisMet: 0.593 ± 0.024	0.533HisAsn: 0.533 ± 0.023	1.341HisPro: 1.341 ± 0.035	0.692HisGln: 0.692 ± 0.026	1.339HisArg: 1.339 ± 0.041	1.121HisSer: 1.121 ± 0.031	0.89HisThr: 0.89 ± 0.025	1.515HisVal: 1.515 ± 0.038	0.358HisTrp: 0.358 ± 0.018	0.591HisTyr: 0.591 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	6.764IleAla: 6.764 ± 0.09	0.639IleCys: 0.639 ± 0.024	3.668IleAsp: 3.668 ± 0.066	3.869IleGlu: 3.869 ± 0.065	1.773IlePhe: 1.773 ± 0.043	4.919IleGly: 4.919 ± 0.081	0.947IleHis: 0.947 ± 0.028	2.436IleIle: 2.436 ± 0.052	1.746IleLys: 1.746 ± 0.048	4.989IleLeu: 4.989 ± 0.075	1.112IleMet: 1.112 ± 0.034	1.665IleAsn: 1.665 ± 0.041	2.524IlePro: 2.524 ± 0.043	1.478IleGln: 1.478 ± 0.034	3.412IleArg: 3.412 ± 0.051	3.482IleSer: 3.482 ± 0.072	3.131IleThr: 3.131 ± 0.048	3.79IleVal: 3.79 ± 0.057	0.785IleTrp: 0.785 ± 0.028	1.24IleTyr: 1.24 ± 0.034	0.0IleXaa: 0.0 ± 0.0
Lys	4.022LysAla: 4.022 ± 0.072	0.226LysCys: 0.226 ± 0.015	1.94LysAsp: 1.94 ± 0.048	1.76LysGlu: 1.76 ± 0.044	1.026LysPhe: 1.026 ± 0.033	2.825LysGly: 2.825 ± 0.051	0.686LysHis: 0.686 ± 0.028	1.889LysIle: 1.889 ± 0.043	1.429LysLys: 1.429 ± 0.046	3.275LysLeu: 3.275 ± 0.059	0.89LysMet: 0.89 ± 0.031	1.003LysAsn: 1.003 ± 0.028	2.017LysPro: 2.017 ± 0.051	1.206LysGln: 1.206 ± 0.035	2.311LysArg: 2.311 ± 0.041	2.025LysSer: 2.025 ± 0.046	2.161LysThr: 2.161 ± 0.042	2.415LysVal: 2.415 ± 0.047	0.442LysTrp: 0.442 ± 0.021	0.753LysTyr: 0.753 ± 0.028	0.001LysXaa: 0.001 ± 0.001
Leu	11.273LeuAla: 11.273 ± 0.129	0.953LeuCys: 0.953 ± 0.033	6.036LeuAsp: 6.036 ± 0.087	5.431LeuGlu: 5.431 ± 0.071	3.609LeuPhe: 3.609 ± 0.071	7.988LeuGly: 7.988 ± 0.111	1.881LeuHis: 1.881 ± 0.039	5.509LeuIle: 5.509 ± 0.079	3.494LeuLys: 3.494 ± 0.056	8.446LeuLeu: 8.446 ± 0.142	2.486LeuMet: 2.486 ± 0.059	3.261LeuAsn: 3.261 ± 0.06	5.128LeuPro: 5.128 ± 0.082	3.181LeuGln: 3.181 ± 0.049	6.581LeuArg: 6.581 ± 0.091	6.917LeuSer: 6.917 ± 0.096	6.099LeuThr: 6.099 ± 0.065	6.415LeuVal: 6.415 ± 0.076	1.217LeuTrp: 1.217 ± 0.037	2.018LeuTyr: 2.018 ± 0.046	0.0LeuXaa: 0.0 ± 0.0
Met	3.129MetAla: 3.129 ± 0.071	0.194MetCys: 0.194 ± 0.014	1.368MetAsp: 1.368 ± 0.039	1.279MetGlu: 1.279 ± 0.036	0.868MetPhe: 0.868 ± 0.027	2.122MetGly: 2.122 ± 0.051	0.457MetHis: 0.457 ± 0.023	1.59MetIle: 1.59 ± 0.044	1.07MetLys: 1.07 ± 0.029	2.554MetLeu: 2.554 ± 0.058	0.773MetMet: 0.773 ± 0.025	0.877MetAsn: 0.877 ± 0.027	1.381MetPro: 1.381 ± 0.037	1.101MetGln: 1.101 ± 0.033	1.813MetArg: 1.813 ± 0.049	1.728MetSer: 1.728 ± 0.042	1.966MetThr: 1.966 ± 0.045	1.877MetVal: 1.877 ± 0.041	0.237MetTrp: 0.237 ± 0.014	0.376MetTyr: 0.376 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	3.429AsnAla: 3.429 ± 0.06	0.313AsnCys: 0.313 ± 0.018	1.812AsnAsp: 1.812 ± 0.083	1.382AsnGlu: 1.382 ± 0.038	1.107AsnPhe: 1.107 ± 0.03	2.888AsnGly: 2.888 ± 0.085	0.604AsnHis: 0.604 ± 0.022	1.588AsnIle: 1.588 ± 0.046	0.872AsnLys: 0.872 ± 0.028	2.957AsnLeu: 2.957 ± 0.052	0.772AsnMet: 0.772 ± 0.026	0.865AsnAsn: 0.865 ± 0.032	2.014AsnPro: 2.014 ± 0.037	0.996AsnGln: 0.996 ± 0.031	1.97AsnArg: 1.97 ± 0.042	1.523AsnSer: 1.523 ± 0.053	1.623AsnThr: 1.623 ± 0.04	2.047AsnVal: 2.047 ± 0.052	0.526AsnTrp: 0.526 ± 0.022	0.751AsnTyr: 0.751 ± 0.027	0.0AsnXaa: 0.0 ± 0.0
Pro	4.867ProAla: 4.867 ± 0.075	0.381ProCys: 0.381 ± 0.019	4.009ProAsp: 4.009 ± 0.068	4.01ProGlu: 4.01 ± 0.074	1.899ProPhe: 1.899 ± 0.042	4.237ProGly: 4.237 ± 0.064	1.017ProHis: 1.017 ± 0.03	2.358ProIle: 2.358 ± 0.05	1.683ProLys: 1.683 ± 0.045	4.146ProLeu: 4.146 ± 0.066	1.294ProMet: 1.294 ± 0.037	1.505ProAsn: 1.505 ± 0.038	1.93ProPro: 1.93 ± 0.05	1.73ProGln: 1.73 ± 0.044	2.506ProArg: 2.506 ± 0.05	2.505ProSer: 2.505 ± 0.052	2.339ProThr: 2.339 ± 0.044	3.939ProVal: 3.939 ± 0.071	0.628ProTrp: 0.628 ± 0.025	1.145ProTyr: 1.145 ± 0.033	0.001ProXaa: 0.001 ± 0.001
Gln	4.405GlnAla: 4.405 ± 0.069	0.237GlnCys: 0.237 ± 0.015	1.988GlnAsp: 1.988 ± 0.043	1.851GlnGlu: 1.851 ± 0.043	1.308GlnPhe: 1.308 ± 0.034	2.828GlnGly: 2.828 ± 0.045	0.703GlnHis: 0.703 ± 0.024	2.441GlnIle: 2.441 ± 0.051	1.295GlnLys: 1.295 ± 0.037	3.284GlnLeu: 3.284 ± 0.064	1.177GlnMet: 1.177 ± 0.037	1.21GlnAsn: 1.21 ± 0.034	1.775GlnPro: 1.775 ± 0.041	1.452GlnGln: 1.452 ± 0.036	2.384GlnArg: 2.384 ± 0.051	2.189GlnSer: 2.189 ± 0.07	2.138GlnThr: 2.138 ± 0.052	2.627GlnVal: 2.627 ± 0.047	0.398GlnTrp: 0.398 ± 0.02	0.692GlnTyr: 0.692 ± 0.025	0.001GlnXaa: 0.001 ± 0.001
Arg	6.983ArgAla: 6.983 ± 0.093	0.499ArgCys: 0.499 ± 0.022	4.029ArgAsp: 4.029 ± 0.06	3.568ArgGlu: 3.568 ± 0.066	2.663ArgPhe: 2.663 ± 0.051	4.188ArgGly: 4.188 ± 0.061	1.456ArgHis: 1.456 ± 0.041	3.798ArgIle: 3.798 ± 0.062	2.509ArgLys: 2.509 ± 0.057	6.693ArgLeu: 6.693 ± 0.085	1.856ArgMet: 1.856 ± 0.048	1.925ArgAsn: 1.925 ± 0.038	3.015ArgPro: 3.015 ± 0.065	2.419ArgGln: 2.419 ± 0.048	4.348ArgArg: 4.348 ± 0.081	3.321ArgSer: 3.321 ± 0.052	2.979ArgThr: 2.979 ± 0.054	4.383ArgVal: 4.383 ± 0.062	0.934ArgTrp: 0.934 ± 0.031	1.564ArgTyr: 1.564 ± 0.043	0.0ArgXaa: 0.0 ± 0.0
Ser	5.938SerAla: 5.938 ± 0.074	0.449SerCys: 0.449 ± 0.02	3.842SerAsp: 3.842 ± 0.087	3.291SerGlu: 3.291 ± 0.062	2.417SerPhe: 2.417 ± 0.053	5.817SerGly: 5.817 ± 0.139	1.148SerHis: 1.148 ± 0.032	2.767SerIle: 2.767 ± 0.065	1.815SerLys: 1.815 ± 0.04	4.998SerLeu: 4.998 ± 0.088	1.363SerMet: 1.363 ± 0.033	1.66SerAsn: 1.66 ± 0.052	2.454SerPro: 2.454 ± 0.045	1.861SerGln: 1.861 ± 0.043	3.24SerArg: 3.24 ± 0.05	2.998SerSer: 2.998 ± 0.087	2.838SerThr: 2.838 ± 0.082	4.181SerVal: 4.181 ± 0.098	0.696SerTrp: 0.696 ± 0.024	1.425SerTyr: 1.425 ± 0.035	0.001SerXaa: 0.001 ± 0.001
Thr	5.926ThrAla: 5.926 ± 0.081	0.482ThrCys: 0.482 ± 0.019	3.312ThrAsp: 3.312 ± 0.06	3.189ThrGlu: 3.189 ± 0.054	1.999ThrPhe: 1.999 ± 0.037	5.501ThrGly: 5.501 ± 0.097	1.212ThrHis: 1.212 ± 0.033	3.063ThrIle: 3.063 ± 0.061	1.575ThrLys: 1.575 ± 0.031	5.924ThrLeu: 5.924 ± 0.082	1.251ThrMet: 1.251 ± 0.037	1.556ThrAsn: 1.556 ± 0.047	3.289ThrPro: 3.289 ± 0.052	1.881ThrGln: 1.881 ± 0.043	3.405ThrArg: 3.405 ± 0.054	3.071ThrSer: 3.071 ± 0.088	3.131ThrThr: 3.131 ± 0.11	4.296ThrVal: 4.296 ± 0.085	0.63ThrTrp: 0.63 ± 0.022	1.301ThrTyr: 1.301 ± 0.032	0.001ThrXaa: 0.001 ± 0.001
Val	7.867ValAla: 7.867 ± 0.08	0.675ValCys: 0.675 ± 0.024	4.351ValAsp: 4.351 ± 0.085	4.565ValGlu: 4.565 ± 0.065	2.913ValPhe: 2.913 ± 0.051	5.276ValGly: 5.276 ± 0.092	1.342ValHis: 1.342 ± 0.035	4.283ValIle: 4.283 ± 0.059	2.249ValLys: 2.249 ± 0.046	7.209ValLeu: 7.209 ± 0.097	1.956ValMet: 1.956 ± 0.047	2.092ValAsn: 2.092 ± 0.041	3.267ValPro: 3.267 ± 0.065	2.386ValGln: 2.386 ± 0.044	3.931ValArg: 3.931 ± 0.061	4.546ValSer: 4.546 ± 0.092	4.601ValThr: 4.601 ± 0.088	5.469ValVal: 5.469 ± 0.081	0.869ValTrp: 0.869 ± 0.031	1.565ValTyr: 1.565 ± 0.038	0.001ValXaa: 0.001 ± 0.001
Trp	1.282TrpAla: 1.282 ± 0.042	0.141TrpCys: 0.141 ± 0.011	0.729TrpAsp: 0.729 ± 0.028	0.657TrpGlu: 0.657 ± 0.025	0.595TrpPhe: 0.595 ± 0.021	1.008TrpGly: 1.008 ± 0.032	0.341TrpHis: 0.341 ± 0.017	0.672TrpIle: 0.672 ± 0.027	0.479TrpLys: 0.479 ± 0.021	1.549TrpLeu: 1.549 ± 0.044	0.397TrpMet: 0.397 ± 0.018	0.466TrpAsn: 0.466 ± 0.02	0.649TrpPro: 0.649 ± 0.027	0.625TrpGln: 0.625 ± 0.023	0.964TrpArg: 0.964 ± 0.032	0.826TrpSer: 0.826 ± 0.026	0.711TrpThr: 0.711 ± 0.024	0.937TrpVal: 0.937 ± 0.03	0.207TrpTrp: 0.207 ± 0.015	0.282TrpTyr: 0.282 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.508TyrAla: 2.508 ± 0.048	0.257TyrCys: 0.257 ± 0.015	1.734TyrAsp: 1.734 ± 0.045	1.341TyrGlu: 1.341 ± 0.035	1.006TyrPhe: 1.006 ± 0.027	2.171TyrGly: 2.171 ± 0.047	0.568TyrHis: 0.568 ± 0.023	0.988TyrIle: 0.988 ± 0.026	0.692TyrLys: 0.692 ± 0.025	2.344TyrLeu: 2.344 ± 0.043	0.47TyrMet: 0.47 ± 0.021	0.692TyrAsn: 0.692 ± 0.02	1.033TyrPro: 1.033 ± 0.033	0.828TyrGln: 0.828 ± 0.029	1.58TyrArg: 1.58 ± 0.034	1.327TyrSer: 1.327 ± 0.036	1.14TyrThr: 1.14 ± 0.028	1.616TyrVal: 1.616 ± 0.038	0.38TyrTrp: 0.38 ± 0.019	0.627TyrTyr: 0.627 ± 0.026	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.001XaaPhe: 0.001 ± 0.001	0.0XaaGly: 0.0 ± 0.0	0.001XaaHis: 0.001 ± 0.001	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.002XaaAsn: 0.002 ± 0.001	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.002XaaArg: 0.002 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.112XaaXaa: 0.112 ± 0.051

Statistics based on 4229 proteins (1272411 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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