Acidithiobacillus caldus (strain SM-1)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Acidithiobacillia; Acidithiobacillales; Acidithiobacillaceae; Acidithiobacillus; Acidithiobacillus caldus

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3062 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F9ZSB5|F9ZSB5_ACICS Uncharacterized protein OS=Acidithiobacillus caldus (strain SM-1) OX=990288 GN=Atc_0515 PE=4 SV=1
MM1 pKa = 6.84STATEE6 pKa = 4.26SMTNLSEE13 pKa = 4.4LPPVMTLTTNAAEE26 pKa = 4.71KK27 pKa = 10.03IKK29 pKa = 10.93SLIEE33 pKa = 4.04EE34 pKa = 4.47EE35 pKa = 4.83GSADD39 pKa = 3.1LKK41 pKa = 11.16LRR43 pKa = 11.84VFVTGGGCSGFQYY56 pKa = 11.01GFTFDD61 pKa = 4.54EE62 pKa = 4.51NVNDD66 pKa = 3.78GDD68 pKa = 4.39TEE70 pKa = 4.39VEE72 pKa = 4.05QHH74 pKa = 6.26GVTLLVDD81 pKa = 3.82PMSYY85 pKa = 10.34QYY87 pKa = 11.57LVGAEE92 pKa = 3.74IDD94 pKa = 3.65YY95 pKa = 11.51SEE97 pKa = 4.53GLEE100 pKa = 4.05GAQFVIKK107 pKa = 10.5NPNATTTCGCGSSFSAA123 pKa = 4.79

Molecular weight: 13.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F9ZU00|F9ZU00_ACICS Exodeoxyribonuclease III OS=Acidithiobacillus caldus (strain SM-1) OX=990288 GN=Atc_2854 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVVHH11 pKa = 6.89RR12 pKa = 11.84KK13 pKa = 7.38RR14 pKa = 11.84THH16 pKa = 5.56GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.39AGRR28 pKa = 11.84LVLKK32 pKa = 10.33RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.39GRR39 pKa = 11.84ARR41 pKa = 11.84LCPP44 pKa = 3.71

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3062

0

3062

904590

20

2005

295.4

32.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.581 ± 0.061

0.901 ± 0.015

5.021 ± 0.038

5.739 ± 0.051

3.553 ± 0.032

8.063 ± 0.043

2.539 ± 0.023

4.716 ± 0.035

2.918 ± 0.032

11.655 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.187 ± 0.023

2.471 ± 0.034

5.425 ± 0.037

4.29 ± 0.038

7.728 ± 0.057

5.37 ± 0.04

4.586 ± 0.029

6.953 ± 0.045

1.729 ± 0.025

2.577 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski