Amino acid dipepetide frequency for Acidithiobacillus caldus (strain SM-1)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	15.002AlaAla: 15.002 ± 0.19	1.062AlaCys: 1.062 ± 0.033	5.483AlaAsp: 5.483 ± 0.075	7.44AlaGlu: 7.44 ± 0.104	3.754AlaPhe: 3.754 ± 0.073	8.884AlaGly: 8.884 ± 0.112	2.628AlaHis: 2.628 ± 0.062	5.669AlaIle: 5.669 ± 0.075	3.424AlaLys: 3.424 ± 0.075	14.45AlaLeu: 14.45 ± 0.188	2.884AlaMet: 2.884 ± 0.06	2.556AlaAsn: 2.556 ± 0.062	5.043AlaPro: 5.043 ± 0.081	5.567AlaGln: 5.567 ± 0.087	8.595AlaArg: 8.595 ± 0.135	5.547AlaSer: 5.547 ± 0.08	5.043AlaThr: 5.043 ± 0.082	8.126AlaVal: 8.126 ± 0.106	1.901AlaTrp: 1.901 ± 0.046	2.747AlaTyr: 2.747 ± 0.063	0.0AlaXaa: 0.0 ± 0.0
Cys	1.086CysAla: 1.086 ± 0.035	0.124CysCys: 0.124 ± 0.015	0.364CysAsp: 0.364 ± 0.019	0.356CysGlu: 0.356 ± 0.021	0.305CysPhe: 0.305 ± 0.017	0.961CysGly: 0.961 ± 0.037	0.258CysHis: 0.258 ± 0.018	0.385CysIle: 0.385 ± 0.023	0.221CysLys: 0.221 ± 0.015	0.918CysLeu: 0.918 ± 0.037	0.169CysMet: 0.169 ± 0.015	0.25CysAsn: 0.25 ± 0.016	0.648CysPro: 0.648 ± 0.028	0.353CysGln: 0.353 ± 0.019	0.757CysArg: 0.757 ± 0.031	0.562CysSer: 0.562 ± 0.026	0.411CysThr: 0.411 ± 0.023	0.531CysVal: 0.531 ± 0.024	0.134CysTrp: 0.134 ± 0.013	0.217CysTyr: 0.217 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	5.647AspAla: 5.647 ± 0.095	0.491AspCys: 0.491 ± 0.027	2.389AspAsp: 2.389 ± 0.053	2.716AspGlu: 2.716 ± 0.063	2.208AspPhe: 2.208 ± 0.053	4.325AspGly: 4.325 ± 0.077	1.212AspHis: 1.212 ± 0.037	2.397AspIle: 2.397 ± 0.057	1.365AspLys: 1.365 ± 0.046	6.279AspLeu: 6.279 ± 0.089	1.036AspMet: 1.036 ± 0.033	1.089AspAsn: 1.089 ± 0.035	3.322AspPro: 3.322 ± 0.06	1.61AspGln: 1.61 ± 0.044	3.849AspArg: 3.849 ± 0.069	2.327AspSer: 2.327 ± 0.048	2.253AspThr: 2.253 ± 0.052	2.988AspVal: 2.988 ± 0.059	1.093AspTrp: 1.093 ± 0.035	1.612AspTyr: 1.612 ± 0.041	0.0AspXaa: 0.0 ± 0.0
Glu	7.023GluAla: 7.023 ± 0.115	0.402GluCys: 0.402 ± 0.021	2.963GluAsp: 2.963 ± 0.056	3.388GluGlu: 3.388 ± 0.072	1.642GluPhe: 1.642 ± 0.044	3.953GluGly: 3.953 ± 0.073	1.659GluHis: 1.659 ± 0.039	3.345GluIle: 3.345 ± 0.067	2.135GluLys: 2.135 ± 0.063	6.04GluLeu: 6.04 ± 0.098	1.304GluMet: 1.304 ± 0.045	1.502GluAsn: 1.502 ± 0.045	2.508GluPro: 2.508 ± 0.06	2.728GluGln: 2.728 ± 0.064	5.372GluArg: 5.372 ± 0.1	2.93GluSer: 2.93 ± 0.062	2.714GluThr: 2.714 ± 0.049	3.793GluVal: 3.793 ± 0.07	0.788GluTrp: 0.788 ± 0.03	1.204GluTyr: 1.204 ± 0.036	0.0GluXaa: 0.0 ± 0.0
Phe	4.105PheAla: 4.105 ± 0.079	0.467PheCys: 0.467 ± 0.02	1.757PheAsp: 1.757 ± 0.047	1.4PheGlu: 1.4 ± 0.038	1.595PhePhe: 1.595 ± 0.045	3.085PheGly: 3.085 ± 0.066	0.925PheHis: 0.925 ± 0.031	1.526PheIle: 1.526 ± 0.046	0.684PheLys: 0.684 ± 0.03	4.409PheLeu: 4.409 ± 0.08	0.689PheMet: 0.689 ± 0.027	0.932PheAsn: 0.932 ± 0.032	1.792PhePro: 1.792 ± 0.046	1.246PheGln: 1.246 ± 0.038	2.345PheArg: 2.345 ± 0.054	2.346PheSer: 2.346 ± 0.056	1.895PheThr: 1.895 ± 0.047	2.527PheVal: 2.527 ± 0.06	0.75PheTrp: 0.75 ± 0.035	1.062PheTyr: 1.062 ± 0.039	0.0PheXaa: 0.0 ± 0.0
Gly	7.759GlyAla: 7.759 ± 0.115	0.761GlyCys: 0.761 ± 0.032	3.782GlyAsp: 3.782 ± 0.065	4.403GlyGlu: 4.403 ± 0.074	3.26GlyPhe: 3.26 ± 0.066	6.372GlyGly: 6.372 ± 0.1	2.068GlyHis: 2.068 ± 0.05	4.621GlyIle: 4.621 ± 0.084	3.291GlyLys: 3.291 ± 0.061	8.91GlyLeu: 8.91 ± 0.103	2.309GlyMet: 2.309 ± 0.054	2.318GlyAsn: 2.318 ± 0.067	3.523GlyPro: 3.523 ± 0.066	3.427GlyGln: 3.427 ± 0.072	5.779GlyArg: 5.779 ± 0.09	4.707GlySer: 4.707 ± 0.087	3.793GlyThr: 3.793 ± 0.074	5.817GlyVal: 5.817 ± 0.094	1.383GlyTrp: 1.383 ± 0.046	2.346GlyTyr: 2.346 ± 0.06	0.0GlyXaa: 0.0 ± 0.0
His	2.444HisAla: 2.444 ± 0.054	0.355HisCys: 0.355 ± 0.019	1.103HisAsp: 1.103 ± 0.035	1.214HisGlu: 1.214 ± 0.036	1.144HisPhe: 1.144 ± 0.036	2.338HisGly: 2.338 ± 0.05	0.755HisHis: 0.755 ± 0.031	1.161HisIle: 1.161 ± 0.035	0.58HisLys: 0.58 ± 0.029	2.993HisLeu: 2.993 ± 0.063	0.537HisMet: 0.537 ± 0.027	0.526HisAsn: 0.526 ± 0.026	1.896HisPro: 1.896 ± 0.043	0.945HisGln: 0.945 ± 0.032	2.02HisArg: 2.02 ± 0.046	1.254HisSer: 1.254 ± 0.036	1.036HisThr: 1.036 ± 0.036	1.413HisVal: 1.413 ± 0.041	0.72HisTrp: 0.72 ± 0.03	0.954HisTyr: 0.954 ± 0.034	0.0HisXaa: 0.0 ± 0.0
Ile	5.945IleAla: 5.945 ± 0.088	0.41IleCys: 0.41 ± 0.022	2.534IleAsp: 2.534 ± 0.058	2.508IleGlu: 2.508 ± 0.054	1.87IlePhe: 1.87 ± 0.051	3.868IleGly: 3.868 ± 0.073	1.354IleHis: 1.354 ± 0.038	1.802IleIle: 1.802 ± 0.056	1.097IleLys: 1.097 ± 0.04	5.803IleLeu: 5.803 ± 0.081	0.681IleMet: 0.681 ± 0.031	1.234IleAsn: 1.234 ± 0.046	2.909IlePro: 2.909 ± 0.059	1.889IleGln: 1.889 ± 0.047	3.344IleArg: 3.344 ± 0.055	2.396IleSer: 2.396 ± 0.06	2.225IleThr: 2.225 ± 0.05	3.484IleVal: 3.484 ± 0.068	0.633IleTrp: 0.633 ± 0.03	1.174IleTyr: 1.174 ± 0.044	0.0IleXaa: 0.0 ± 0.0
Lys	3.693LysAla: 3.693 ± 0.079	0.148LysCys: 0.148 ± 0.013	1.648LysAsp: 1.648 ± 0.049	1.716LysGlu: 1.716 ± 0.055	0.642LysPhe: 0.642 ± 0.031	2.358LysGly: 2.358 ± 0.056	0.627LysHis: 0.627 ± 0.029	1.498LysIle: 1.498 ± 0.047	1.231LysLys: 1.231 ± 0.053	2.67LysLeu: 2.67 ± 0.056	0.659LysMet: 0.659 ± 0.03	0.957LysAsn: 0.957 ± 0.041	1.705LysPro: 1.705 ± 0.05	1.005LysGln: 1.005 ± 0.035	2.124LysArg: 2.124 ± 0.06	1.647LysSer: 1.647 ± 0.049	1.843LysThr: 1.843 ± 0.048	2.077LysVal: 2.077 ± 0.058	0.304LysTrp: 0.304 ± 0.019	0.625LysTyr: 0.625 ± 0.033	0.0LysXaa: 0.0 ± 0.0
Leu	14.572LeuAla: 14.572 ± 0.169	1.096LeuCys: 1.096 ± 0.035	6.174LeuAsp: 6.174 ± 0.088	6.909LeuGlu: 6.909 ± 0.108	3.875LeuPhe: 3.875 ± 0.076	9.556LeuGly: 9.556 ± 0.122	2.865LeuHis: 2.865 ± 0.066	4.451LeuIle: 4.451 ± 0.075	2.977LeuLys: 2.977 ± 0.072	13.391LeuLeu: 13.391 ± 0.187	2.205LeuMet: 2.205 ± 0.051	2.451LeuAsn: 2.451 ± 0.062	6.945LeuPro: 6.945 ± 0.097	5.3LeuGln: 5.3 ± 0.096	10.237LeuArg: 10.237 ± 0.15	6.637LeuSer: 6.637 ± 0.087	4.745LeuThr: 4.745 ± 0.071	7.641LeuVal: 7.641 ± 0.114	1.959LeuTrp: 1.959 ± 0.062	2.561LeuTyr: 2.561 ± 0.069	0.0LeuXaa: 0.0 ± 0.0
Met	2.841MetAla: 2.841 ± 0.058	0.098MetCys: 0.098 ± 0.011	1.317MetAsp: 1.317 ± 0.047	1.35MetGlu: 1.35 ± 0.041	0.476MetPhe: 0.476 ± 0.026	1.807MetGly: 1.807 ± 0.052	0.538MetHis: 0.538 ± 0.025	0.906MetIle: 0.906 ± 0.033	0.71MetLys: 0.71 ± 0.03	2.162MetLeu: 2.162 ± 0.051	0.443MetMet: 0.443 ± 0.021	0.713MetAsn: 0.713 ± 0.029	1.299MetPro: 1.299 ± 0.041	0.882MetGln: 0.882 ± 0.035	1.539MetArg: 1.539 ± 0.039	1.375MetSer: 1.375 ± 0.038	1.223MetThr: 1.223 ± 0.035	1.698MetVal: 1.698 ± 0.043	0.179MetTrp: 0.179 ± 0.016	0.307MetTyr: 0.307 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	2.776AsnAla: 2.776 ± 0.058	0.218AsnCys: 0.218 ± 0.017	1.193AsnAsp: 1.193 ± 0.04	0.967AsnGlu: 0.967 ± 0.042	0.954AsnPhe: 0.954 ± 0.036	2.136AsnGly: 2.136 ± 0.064	0.537AsnHis: 0.537 ± 0.023	1.26AsnIle: 1.26 ± 0.042	0.61AsnLys: 0.61 ± 0.034	2.922AsnLeu: 2.922 ± 0.061	0.49AsnMet: 0.49 ± 0.026	0.701AsnAsn: 0.701 ± 0.035	2.017AsnPro: 2.017 ± 0.056	0.894AsnGln: 0.894 ± 0.039	1.927AsnArg: 1.927 ± 0.047	1.12AsnSer: 1.12 ± 0.043	1.27AsnThr: 1.27 ± 0.041	1.566AsnVal: 1.566 ± 0.046	0.416AsnTrp: 0.416 ± 0.021	0.732AsnTyr: 0.732 ± 0.037	0.0AsnXaa: 0.0 ± 0.0
Pro	6.013ProAla: 6.013 ± 0.092	0.403ProCys: 0.403 ± 0.022	3.075ProAsp: 3.075 ± 0.063	4.287ProGlu: 4.287 ± 0.076	1.872ProPhe: 1.872 ± 0.051	4.703ProGly: 4.703 ± 0.078	1.27ProHis: 1.27 ± 0.044	2.328ProIle: 2.328 ± 0.061	1.778ProLys: 1.778 ± 0.056	5.536ProLeu: 5.536 ± 0.084	1.331ProMet: 1.331 ± 0.039	1.277ProAsn: 1.277 ± 0.041	2.677ProPro: 2.677 ± 0.085	2.34ProGln: 2.34 ± 0.048	3.239ProArg: 3.239 ± 0.062	2.991ProSer: 2.991 ± 0.065	2.397ProThr: 2.397 ± 0.055	4.084ProVal: 4.084 ± 0.069	1.104ProTrp: 1.104 ± 0.037	1.542ProTyr: 1.542 ± 0.046	0.0ProXaa: 0.0 ± 0.0
Gln	5.073GlnAla: 5.073 ± 0.085	0.319GlnCys: 0.319 ± 0.019	1.946GlnAsp: 1.946 ± 0.05	2.68GlnGlu: 2.68 ± 0.062	1.114GlnPhe: 1.114 ± 0.038	3.39GlnGly: 3.39 ± 0.069	1.117GlnHis: 1.117 ± 0.038	2.146GlnIle: 2.146 ± 0.056	1.547GlnLys: 1.547 ± 0.046	4.137GlnLeu: 4.137 ± 0.076	1.006GlnMet: 1.006 ± 0.033	1.101GlnAsn: 1.101 ± 0.041	1.942GlnPro: 1.942 ± 0.049	2.106GlnGln: 2.106 ± 0.06	4.036GlnArg: 4.036 ± 0.085	2.506GlnSer: 2.506 ± 0.055	1.907GlnThr: 1.907 ± 0.05	2.967GlnVal: 2.967 ± 0.063	0.857GlnTrp: 0.857 ± 0.031	1.005GlnTyr: 1.005 ± 0.037	0.0GlnXaa: 0.0 ± 0.0
Arg	7.944ArgAla: 7.944 ± 0.103	0.713ArgCys: 0.713 ± 0.033	3.995ArgAsp: 3.995 ± 0.074	5.17ArgGlu: 5.17 ± 0.091	3.007ArgPhe: 3.007 ± 0.063	5.11ArgGly: 5.11 ± 0.086	2.268ArgHis: 2.268 ± 0.057	4.042ArgIle: 4.042 ± 0.063	2.364ArgLys: 2.364 ± 0.053	9.316ArgLeu: 9.316 ± 0.15	1.647ArgMet: 1.647 ± 0.04	1.795ArgAsn: 1.795 ± 0.042	3.721ArgPro: 3.721 ± 0.061	3.71ArgGln: 3.71 ± 0.066	6.835ArgArg: 6.835 ± 0.121	4.048ArgSer: 4.048 ± 0.075	2.612ArgThr: 2.612 ± 0.058	5.16ArgVal: 5.16 ± 0.087	1.519ArgTrp: 1.519 ± 0.047	2.3ArgTyr: 2.3 ± 0.053	0.0ArgXaa: 0.0 ± 0.0
Ser	6.227SerAla: 6.227 ± 0.093	0.525SerCys: 0.525 ± 0.024	2.407SerAsp: 2.407 ± 0.055	2.562SerGlu: 2.562 ± 0.06	2.005SerPhe: 2.005 ± 0.048	5.095SerGly: 5.095 ± 0.092	1.256SerHis: 1.256 ± 0.038	2.449SerIle: 2.449 ± 0.053	1.384SerLys: 1.384 ± 0.038	6.4SerLeu: 6.4 ± 0.088	1.39SerMet: 1.39 ± 0.044	1.364SerAsn: 1.364 ± 0.049	3.009SerPro: 3.009 ± 0.058	2.011SerGln: 2.011 ± 0.054	3.639SerArg: 3.639 ± 0.071	3.168SerSer: 3.168 ± 0.085	2.662SerThr: 2.662 ± 0.067	3.787SerVal: 3.787 ± 0.066	0.992SerTrp: 0.992 ± 0.034	1.37SerTyr: 1.37 ± 0.041	0.0SerXaa: 0.0 ± 0.0
Thr	5.303ThrAla: 5.303 ± 0.082	0.354ThrCys: 0.354 ± 0.022	2.174ThrAsp: 2.174 ± 0.05	2.242ThrGlu: 2.242 ± 0.048	1.548ThrPhe: 1.548 ± 0.048	4.109ThrGly: 4.109 ± 0.078	1.105ThrHis: 1.105 ± 0.035	2.265ThrIle: 2.265 ± 0.057	1.121ThrLys: 1.121 ± 0.039	5.971ThrLeu: 5.971 ± 0.082	0.931ThrMet: 0.931 ± 0.041	1.02ThrAsn: 1.02 ± 0.042	3.053ThrPro: 3.053 ± 0.059	1.878ThrGln: 1.878 ± 0.047	2.886ThrArg: 2.886 ± 0.053	2.165ThrSer: 2.165 ± 0.058	2.356ThrThr: 2.356 ± 0.071	3.638ThrVal: 3.638 ± 0.07	0.698ThrTrp: 0.698 ± 0.032	1.046ThrTyr: 1.046 ± 0.039	0.0ThrXaa: 0.0 ± 0.0
Val	8.07ValAla: 8.07 ± 0.11	0.601ValCys: 0.601 ± 0.027	4.0ValAsp: 4.0 ± 0.069	4.167ValGlu: 4.167 ± 0.076	2.551ValPhe: 2.551 ± 0.051	5.174ValGly: 5.174 ± 0.083	1.762ValHis: 1.762 ± 0.049	3.065ValIle: 3.065 ± 0.063	1.674ValLys: 1.674 ± 0.051	8.606ValLeu: 8.606 ± 0.116	1.37ValMet: 1.37 ± 0.042	1.694ValAsn: 1.694 ± 0.045	3.817ValPro: 3.817 ± 0.073	2.875ValGln: 2.875 ± 0.063	4.827ValArg: 4.827 ± 0.081	3.706ValSer: 3.706 ± 0.07	3.454ValThr: 3.454 ± 0.066	5.426ValVal: 5.426 ± 0.096	0.994ValTrp: 0.994 ± 0.033	1.7ValTyr: 1.7 ± 0.043	0.0ValXaa: 0.0 ± 0.0
Trp	1.484TrpAla: 1.484 ± 0.046	0.176TrpCys: 0.176 ± 0.015	0.741TrpAsp: 0.741 ± 0.03	0.825TrpGlu: 0.825 ± 0.03	0.673TrpPhe: 0.673 ± 0.031	1.109TrpGly: 1.109 ± 0.043	0.453TrpHis: 0.453 ± 0.023	0.876TrpIle: 0.876 ± 0.034	0.476TrpLys: 0.476 ± 0.023	2.366TrpLeu: 2.366 ± 0.062	0.41TrpMet: 0.41 ± 0.022	0.475TrpAsn: 0.475 ± 0.025	0.873TrpPro: 0.873 ± 0.035	1.057TrpGln: 1.057 ± 0.038	1.52TrpArg: 1.52 ± 0.041	0.936TrpSer: 0.936 ± 0.03	0.839TrpThr: 0.839 ± 0.033	1.133TrpVal: 1.133 ± 0.037	0.397TrpTrp: 0.397 ± 0.027	0.472TrpTyr: 0.472 ± 0.024	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.8TyrAla: 2.8 ± 0.062	0.283TyrCys: 0.283 ± 0.019	1.163TyrAsp: 1.163 ± 0.039	1.094TyrGlu: 1.094 ± 0.037	1.038TyrPhe: 1.038 ± 0.038	2.298TyrGly: 2.298 ± 0.056	0.729TyrHis: 0.729 ± 0.028	0.971TyrIle: 0.971 ± 0.037	0.509TyrLys: 0.509 ± 0.024	3.27TyrLeu: 3.27 ± 0.062	0.373TyrMet: 0.373 ± 0.024	0.751TyrAsn: 0.751 ± 0.039	1.548TyrPro: 1.548 ± 0.047	1.078TyrGln: 1.078 ± 0.042	2.408TyrArg: 2.408 ± 0.058	1.334TyrSer: 1.334 ± 0.047	1.186TyrThr: 1.186 ± 0.042	1.674TyrVal: 1.674 ± 0.042	0.471TyrTrp: 0.471 ± 0.023	0.793TyrTyr: 0.793 ± 0.036	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3062 proteins (904590 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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