Mycoplasma haemocanis (strain Illinois)
Average proteome isoelectric point is 7.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1175 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H6N6P7|H6N6P7_MYCHN Uncharacterized protein OS=Mycoplasma haemocanis (strain Illinois) OX=1111676 GN=MHC_02265 PE=4 SV=1
MM1 pKa = 7.54 QDD3 pKa = 3.09 INRR6 pKa = 11.84 KK7 pKa = 9.55 GPDD10 pKa = 3.86 NIPEE14 pKa = 4.3 GLKK17 pKa = 9.75 QAYY20 pKa = 6.95 EE21 pKa = 4.35 WKK23 pKa = 9.71 DD24 pKa = 3.65 QPHH27 pKa = 5.39 MAEE30 pKa = 3.26 RR31 pKa = 11.84 WAALFCEE38 pKa = 5.57 DD39 pKa = 4.36 GPQDD43 pKa = 4.15 INEE46 pKa = 4.35 TNYY49 pKa = 10.83 KK50 pKa = 10.39 DD51 pKa = 3.63 YY52 pKa = 11.44 QEE54 pKa = 4.4 YY55 pKa = 10.49 CNGYY59 pKa = 9.65
Molecular weight: 7.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.106
IPC2_protein 4.457
IPC_protein 4.304
Toseland 4.126
ProMoST 4.431
Dawson 4.266
Bjellqvist 4.418
Wikipedia 4.164
Rodwell 4.139
Grimsley 4.05
Solomon 4.253
Lehninger 4.202
Nozaki 4.38
DTASelect 4.546
Thurlkill 4.164
EMBOSS 4.177
Sillero 4.418
Patrickios 1.99
IPC_peptide 4.253
IPC2_peptide 4.406
IPC2.peptide.svr19 4.323
Protein with the highest isoelectric point:
>tr|H6N5I4|H6N5I4_MYCHN Ribonucleotide reductase stimulatory protein OS=Mycoplasma haemocanis (strain Illinois) OX=1111676 GN=nrdI PE=4 SV=1
MM1 pKa = 7.32 AVSPRR6 pKa = 11.84 RR7 pKa = 11.84 VSKK10 pKa = 10.51 SRR12 pKa = 11.84 KK13 pKa = 8.17 GMRR16 pKa = 11.84 NSHH19 pKa = 6.21 SALKK23 pKa = 7.59 TTSLVVCHH31 pKa = 6.12 NCSRR35 pKa = 11.84 HH36 pKa = 5.29 IKK38 pKa = 8.63 SHH40 pKa = 5.2 SVCEE44 pKa = 3.76 CGYY47 pKa = 10.17 YY48 pKa = 10.09 KK49 pKa = 10.65 GRR51 pKa = 11.84 QIIKK55 pKa = 10.17 KK56 pKa = 9.9
Molecular weight: 6.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.328
IPC2_protein 9.78
IPC_protein 10.218
Toseland 10.789
ProMoST 10.423
Dawson 10.862
Bjellqvist 10.526
Wikipedia 11.008
Rodwell 11.316
Grimsley 10.891
Solomon 10.935
Lehninger 10.921
Nozaki 10.789
DTASelect 10.511
Thurlkill 10.774
EMBOSS 11.169
Sillero 10.789
Patrickios 11.082
IPC_peptide 10.95
IPC2_peptide 9.706
IPC2.peptide.svr19 8.509
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1175
0
1175
284213
29
2239
241.9
27.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.041 ± 0.057
1.487 ± 0.026
5.916 ± 0.053
7.257 ± 0.061
4.463 ± 0.078
6.102 ± 0.071
1.445 ± 0.025
6.331 ± 0.076
10.81 ± 0.084
9.324 ± 0.076
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.485 ± 0.024
5.711 ± 0.052
2.63 ± 0.033
2.769 ± 0.038
3.43 ± 0.049
9.683 ± 0.086
5.303 ± 0.067
5.352 ± 0.06
1.924 ± 0.031
3.539 ± 0.048
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here