Zootermopsis nevadensis (Dampwood termite)
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 14539 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A067R0Z9|A0A067R0Z9_ZOONE PDZ domain-containing protein OS=Zootermopsis nevadensis OX=136037 GN=L798_08776 PE=4 SV=1
MM1 pKa = 7.13 VVLSNFPPPADD12 pKa = 4.5 GIKK15 pKa = 10.1 HH16 pKa = 5.53 EE17 pKa = 4.36 QPSTTVHH24 pKa = 5.87 INDD27 pKa = 3.77 RR28 pKa = 11.84 EE29 pKa = 4.15 IGIGTLFITEE39 pKa = 4.52 SRR41 pKa = 11.84 LSWVNSSSGQGFSLEE56 pKa = 4.15 YY57 pKa = 10.16 PHH59 pKa = 7.21 IALHH63 pKa = 5.9 AVSRR67 pKa = 11.84 DD68 pKa = 3.26 LQAYY72 pKa = 8.88 PSEE75 pKa = 4.1 CLYY78 pKa = 11.32 VLVDD82 pKa = 4.82 SEE84 pKa = 4.84 IDD86 pKa = 3.47 PDD88 pKa = 3.95 NQLHH92 pKa = 6.16 SSSGDD97 pKa = 3.23 EE98 pKa = 3.95 DD99 pKa = 4.76 SEE101 pKa = 5.27 VGVQDD106 pKa = 3.36 MTEE109 pKa = 3.86 MRR111 pKa = 11.84 FVPDD115 pKa = 3.98 DD116 pKa = 3.79 KK117 pKa = 11.91 GMLDD121 pKa = 3.61 AMFHH125 pKa = 6.44 AMSEE129 pKa = 4.52 CQSLHH134 pKa = 7.3 PDD136 pKa = 3.6 PQDD139 pKa = 3.24 SFSEE143 pKa = 4.18 DD144 pKa = 3.19 EE145 pKa = 5.09 EE146 pKa = 4.55 IFEE149 pKa = 5.47 DD150 pKa = 4.68 ADD152 pKa = 3.88 DD153 pKa = 4.55 EE154 pKa = 4.43 EE155 pKa = 6.46 GEE157 pKa = 4.32 YY158 pKa = 10.86 HH159 pKa = 6.84 LGEE162 pKa = 4.81 GDD164 pKa = 3.54 ASVVSHH170 pKa = 6.11 NNSYY174 pKa = 10.88 EE175 pKa = 3.91 VTANGNDD182 pKa = 3.83 AEE184 pKa = 4.45 DD185 pKa = 4.62 EE186 pKa = 4.16 PMEE189 pKa = 4.33 MDD191 pKa = 3.3 TGQFEE196 pKa = 4.67 DD197 pKa = 5.11 ADD199 pKa = 4.14 DD200 pKa = 3.75
Molecular weight: 22.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.737
IPC2_protein 3.757
IPC_protein 3.757
Toseland 3.554
ProMoST 3.884
Dawson 3.745
Bjellqvist 3.91
Wikipedia 3.656
Rodwell 3.579
Grimsley 3.452
Solomon 3.732
Lehninger 3.694
Nozaki 3.846
DTASelect 4.062
Thurlkill 3.592
EMBOSS 3.668
Sillero 3.872
Patrickios 1.85
IPC_peptide 3.732
IPC2_peptide 3.859
IPC2.peptide.svr19 3.803
Protein with the highest isoelectric point:
>tr|A0A067QFH3|A0A067QFH3_ZOONE Uncharacterized protein OS=Zootermopsis nevadensis OX=136037 GN=L798_04436 PE=4 SV=1
AA1 pKa = 7.73 LPRR4 pKa = 11.84 LAALPRR10 pKa = 11.84 LAALPRR16 pKa = 11.84 LAALPRR22 pKa = 11.84 LAALPRR28 pKa = 11.84 LAALPRR34 pKa = 11.84 LAALPRR40 pKa = 11.84 LAALPRR46 pKa = 11.84 LAALPRR52 pKa = 11.84 LAALPRR58 pKa = 11.84 LAALPRR64 pKa = 11.84 LAALPRR70 pKa = 11.84 LAALPRR76 pKa = 11.84 LAALPRR82 pKa = 11.84 LAALPRR88 pKa = 11.84 LAALPRR94 pKa = 11.84 LAALPRR100 pKa = 11.84 LAALPRR106 pKa = 11.84 LAALPRR112 pKa = 11.84 LAALPRR118 pKa = 11.84 LAALPRR124 pKa = 11.84 LAALPRR130 pKa = 11.84 LAALPRR136 pKa = 11.84 LAALPRR142 pKa = 11.84 LAYY145 pKa = 9.5 PP146 pKa = 4.41
Molecular weight: 15.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.525
IPC2_protein 11.418
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.544
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.266
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.254
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14539
0
14539
6045344
30
5553
415.8
46.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.151 ± 0.018
2.204 ± 0.016
5.281 ± 0.016
6.521 ± 0.026
3.738 ± 0.016
5.985 ± 0.028
2.871 ± 0.015
4.988 ± 0.019
5.861 ± 0.027
9.342 ± 0.026
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.35 ± 0.009
4.397 ± 0.015
5.11 ± 0.027
4.39 ± 0.021
5.566 ± 0.019
8.499 ± 0.029
5.814 ± 0.018
6.782 ± 0.018
1.148 ± 0.007
2.971 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here