Epichloe festucae virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes; Ghabrivirales; Totiviridae; Victorivirus

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q1M2S1|Q1M2S1_9VIRU Putative coat protein OS=Epichloe festucae virus 1 OX=382962 GN=CP PE=4 SV=1
MM1 pKa = 7.44MSTAQTQNFQVRR13 pKa = 11.84HH14 pKa = 5.85LAPLTGAVADD24 pKa = 4.11PVGGLLQRR32 pKa = 11.84GKK34 pKa = 10.56DD35 pKa = 3.51FRR37 pKa = 11.84RR38 pKa = 11.84YY39 pKa = 9.5RR40 pKa = 11.84SGLSSEE46 pKa = 4.03IPGPGRR52 pKa = 11.84TFVEE56 pKa = 4.27YY57 pKa = 10.45RR58 pKa = 11.84SIFYY62 pKa = 10.41EE63 pKa = 4.06VGRR66 pKa = 11.84HH67 pKa = 5.01YY68 pKa = 11.66GNISDD73 pKa = 5.38ALATPTPAAVRR84 pKa = 11.84IDD86 pKa = 3.62CSHH89 pKa = 7.65TINEE93 pKa = 4.08TQAANFEE100 pKa = 4.33GLARR104 pKa = 11.84QYY106 pKa = 11.73SNFASQWEE114 pKa = 4.18KK115 pKa = 10.7MDD117 pKa = 3.44LCGIAEE123 pKa = 4.08RR124 pKa = 11.84LARR127 pKa = 11.84AIATQSVFGGVTTTHH142 pKa = 5.66MRR144 pKa = 11.84AGQPLRR150 pKa = 11.84VVALGTLDD158 pKa = 3.82SPQTASTSSVFIPRR172 pKa = 11.84TVDD175 pKa = 3.29SVGSDD180 pKa = 2.89HH181 pKa = 7.03VFAVLCAAANGEE193 pKa = 4.31GASVSTDD200 pKa = 3.2VLRR203 pKa = 11.84LDD205 pKa = 3.95VSNNAPLVPAVAGAPLATACVEE227 pKa = 4.0ALRR230 pKa = 11.84VLGANMEE237 pKa = 4.12EE238 pKa = 4.62SGAGDD243 pKa = 4.1LFAYY247 pKa = 10.12AVTRR251 pKa = 11.84GIHH254 pKa = 5.2RR255 pKa = 11.84VVTVVSHH262 pKa = 5.84TDD264 pKa = 2.73EE265 pKa = 5.1GGILRR270 pKa = 11.84DD271 pKa = 4.06LLRR274 pKa = 11.84HH275 pKa = 5.8DD276 pKa = 4.73AFRR279 pKa = 11.84VPYY282 pKa = 10.31GGINASLRR290 pKa = 11.84HH291 pKa = 5.31YY292 pKa = 9.18PALPSAAGSHH302 pKa = 6.11EE303 pKa = 4.25SSIAAWVDD311 pKa = 4.47GIALKK316 pKa = 9.22TAAVAAHH323 pKa = 7.18CDD325 pKa = 3.59PLVPGVGGMYY335 pKa = 7.99PTVLVASKK343 pKa = 11.04GSISPPGTPEE353 pKa = 4.35GDD355 pKa = 3.47VSPDD359 pKa = 3.35DD360 pKa = 3.81ARR362 pKa = 11.84SLGRR366 pKa = 11.84QLSADD371 pKa = 3.07IGRR374 pKa = 11.84FAPNYY379 pKa = 9.59IRR381 pKa = 11.84GLSALFGLQGYY392 pKa = 6.15TAIAEE397 pKa = 4.15RR398 pKa = 11.84HH399 pKa = 5.06LCATAGHH406 pKa = 5.72YY407 pKa = 10.66LEE409 pKa = 5.6EE410 pKa = 4.41NSATNRR416 pKa = 11.84HH417 pKa = 5.95LLHH420 pKa = 6.18KK421 pKa = 9.49TIAPYY426 pKa = 10.09FWVEE430 pKa = 3.89PTSLIPRR437 pKa = 11.84DD438 pKa = 3.77FLGTSAEE445 pKa = 4.23SEE447 pKa = 4.04NYY449 pKa = 8.32AAKK452 pKa = 10.48VSPGCVAHH460 pKa = 6.93EE461 pKa = 3.8PLFEE465 pKa = 4.31KK466 pKa = 10.36FAPLAQGTTMTCATAGFKK484 pKa = 9.69MRR486 pKa = 11.84TARR489 pKa = 11.84TSGYY493 pKa = 9.99VCSQAAAPASLAMIQLKK510 pKa = 9.86QFDD513 pKa = 4.14TQSVVLAGDD522 pKa = 3.36QGPISGDD529 pKa = 3.15VVTKK533 pKa = 10.43HH534 pKa = 5.97RR535 pKa = 11.84ASSPLSSYY543 pKa = 10.78LWVRR547 pKa = 11.84GQSALPAPAEE557 pKa = 4.25FINTQGCYY565 pKa = 8.63GAKK568 pKa = 9.7IKK570 pKa = 10.94LVDD573 pKa = 3.86WTDD576 pKa = 3.21DD577 pKa = 3.38WDD579 pKa = 4.29PEE581 pKa = 4.47STDD584 pKa = 3.45VPRR587 pKa = 11.84PDD589 pKa = 3.24QFATGVVAWRR599 pKa = 11.84VSLPTGLPTGPSNAADD615 pKa = 3.79RR616 pKa = 11.84QARR619 pKa = 11.84RR620 pKa = 11.84ARR622 pKa = 11.84SRR624 pKa = 11.84GAIALAQALNRR635 pKa = 11.84ARR637 pKa = 11.84AFGEE641 pKa = 4.47GASPTMEE648 pKa = 4.03VSDD651 pKa = 4.24VPPDD655 pKa = 3.93LGLADD660 pKa = 4.84LGHH663 pKa = 6.59YY664 pKa = 10.12DD665 pKa = 3.11HH666 pKa = 7.23TGFRR670 pKa = 11.84EE671 pKa = 3.93HH672 pKa = 7.42RR673 pKa = 11.84SDD675 pKa = 3.39PGKK678 pKa = 10.57AQEE681 pKa = 4.62TGDD684 pKa = 4.24GNPSPTPTLLRR695 pKa = 11.84GAAMPPTEE703 pKa = 3.78QHH705 pKa = 6.31KK706 pKa = 9.45PQGGPRR712 pKa = 11.84LPAPPPTMGRR722 pKa = 11.84AAGPVGPPSTPAPPPPAPVIAPPTVSDD749 pKa = 4.28PPPMPEE755 pKa = 3.73AATGAEE761 pKa = 4.39PVPQAA766 pKa = 4.04

Molecular weight:
80.3 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q1M2S1|Q1M2S1_9VIRU Putative coat protein OS=Epichloe festucae virus 1 OX=382962 GN=CP PE=4 SV=1
MM1 pKa = 7.55IEE3 pKa = 3.94STVADD8 pKa = 4.17RR9 pKa = 11.84ANAAGSVGRR18 pKa = 11.84YY19 pKa = 9.4LEE21 pKa = 4.35ALLPSEE27 pKa = 4.2YY28 pKa = 10.09TRR30 pKa = 11.84VVSTLPFDD38 pKa = 3.8HH39 pKa = 6.37QVSFVYY45 pKa = 10.27RR46 pKa = 11.84PSWRR50 pKa = 11.84HH51 pKa = 5.14HH52 pKa = 6.54KK53 pKa = 8.77PTEE56 pKa = 4.0LQRR59 pKa = 11.84VAAAFLLPRR68 pKa = 11.84VPVQVSYY75 pKa = 11.13SAGSILTLLNATLPNLPPRR94 pKa = 11.84HH95 pKa = 6.11TYY97 pKa = 8.72HH98 pKa = 7.15AGWSCDD104 pKa = 2.97SDD106 pKa = 3.55ATRR109 pKa = 11.84KK110 pKa = 9.78AFPLKK115 pKa = 10.69ANPAASNKK123 pKa = 8.79VNVYY127 pKa = 10.31LSEE130 pKa = 3.87VCYY133 pKa = 10.4DD134 pKa = 3.9LRR136 pKa = 11.84RR137 pKa = 11.84TNPGEE142 pKa = 4.0AMAAEE147 pKa = 4.57AALQRR152 pKa = 11.84VRR154 pKa = 11.84SSGSVYY160 pKa = 10.56NDD162 pKa = 2.77QATAVVLYY170 pKa = 7.78GFCLARR176 pKa = 11.84NGLPGAYY183 pKa = 9.84DD184 pKa = 3.06IALALLLNPDD194 pKa = 3.73YY195 pKa = 11.1AKK197 pKa = 10.96SLTGVLKK204 pKa = 10.09ATGGNATAVGSLLVEE219 pKa = 4.5ANTLLGRR226 pKa = 11.84DD227 pKa = 3.85VAPVDD232 pKa = 3.83LGKK235 pKa = 10.38EE236 pKa = 4.14FNMRR240 pKa = 11.84TQLRR244 pKa = 11.84LARR247 pKa = 11.84EE248 pKa = 4.03NMAEE252 pKa = 4.09YY253 pKa = 9.98PPDD256 pKa = 3.5VLRR259 pKa = 11.84ATVRR263 pKa = 11.84RR264 pKa = 11.84VLLDD268 pKa = 3.2EE269 pKa = 4.07LTRR272 pKa = 11.84RR273 pKa = 11.84AGSYY277 pKa = 10.01EE278 pKa = 4.08LDD280 pKa = 3.63YY281 pKa = 11.2PSLEE285 pKa = 4.25EE286 pKa = 4.1HH287 pKa = 6.8WDD289 pKa = 3.81SRR291 pKa = 11.84WAWAVNGAQGGAITDD306 pKa = 3.9KK307 pKa = 10.91GLEE310 pKa = 4.18SAPRR314 pKa = 11.84PPGATRR320 pKa = 11.84EE321 pKa = 4.07YY322 pKa = 9.88RR323 pKa = 11.84RR324 pKa = 11.84SWLEE328 pKa = 3.65RR329 pKa = 11.84VEE331 pKa = 4.12EE332 pKa = 4.21DD333 pKa = 5.49PRR335 pKa = 11.84PQWDD339 pKa = 3.5GTTEE343 pKa = 3.89VSASSKK349 pKa = 10.62LEE351 pKa = 3.94HH352 pKa = 6.53GKK354 pKa = 7.98TRR356 pKa = 11.84AIFACDD362 pKa = 3.2TVNYY366 pKa = 10.14LAFEE370 pKa = 4.5HH371 pKa = 6.76LMSTVEE377 pKa = 4.06ANWRR381 pKa = 11.84GVRR384 pKa = 11.84VVLNPGKK391 pKa = 10.15GGNMGMAMRR400 pKa = 11.84VQAARR405 pKa = 11.84RR406 pKa = 11.84RR407 pKa = 11.84CGVSLMLDD415 pKa = 3.2YY416 pKa = 11.57DD417 pKa = 4.5DD418 pKa = 5.99FNSHH422 pKa = 5.4HH423 pKa = 7.22TISAMQIVVEE433 pKa = 4.56EE434 pKa = 4.46VCSITGYY441 pKa = 11.11DD442 pKa = 3.48PDD444 pKa = 3.59LAARR448 pKa = 11.84LVASLDD454 pKa = 3.42KK455 pKa = 11.09QYY457 pKa = 9.7ITLAGRR463 pKa = 11.84RR464 pKa = 11.84KK465 pKa = 10.21LSVGTLMSGHH475 pKa = 6.91RR476 pKa = 11.84CTTFFNSVLNAVYY489 pKa = 10.43VRR491 pKa = 11.84LEE493 pKa = 4.13LGEE496 pKa = 4.22PFYY499 pKa = 11.48SEE501 pKa = 4.26TVSLHH506 pKa = 5.96VGDD509 pKa = 4.41DD510 pKa = 3.37VYY512 pKa = 10.99MGVRR516 pKa = 11.84DD517 pKa = 4.23YY518 pKa = 11.86VSAAYY523 pKa = 9.86VIQRR527 pKa = 11.84LADD530 pKa = 3.29SPLRR534 pKa = 11.84MNALKK539 pKa = 10.59QSVGHH544 pKa = 6.11VSTEE548 pKa = 3.76FLRR551 pKa = 11.84IATGARR557 pKa = 11.84DD558 pKa = 3.57CYY560 pKa = 10.89GYY562 pKa = 10.97LSRR565 pKa = 11.84AVASIVSGNWVNDD578 pKa = 3.31TALAPFEE585 pKa = 4.52GLAAMVASARR595 pKa = 11.84SLANRR600 pKa = 11.84GKK602 pKa = 9.19TNLAPLLLVSSVLRR616 pKa = 11.84IAKK619 pKa = 9.69LGKK622 pKa = 8.53RR623 pKa = 11.84HH624 pKa = 5.43YY625 pKa = 10.56GKK627 pKa = 10.62VSSLLDD633 pKa = 3.32GTVALGNGPQYY644 pKa = 11.27SCGAHH649 pKa = 5.51MRR651 pKa = 11.84WCNPVVVRR659 pKa = 11.84DD660 pKa = 4.03TPDD663 pKa = 2.67PWGYY667 pKa = 9.12TEE669 pKa = 5.9LPLEE673 pKa = 4.36SVTAYY678 pKa = 10.69LSRR681 pKa = 11.84AASPLEE687 pKa = 3.91WEE689 pKa = 4.6VLGEE693 pKa = 4.01AGISVTEE700 pKa = 3.89QMAAASYY707 pKa = 9.53RR708 pKa = 11.84KK709 pKa = 8.18TYY711 pKa = 11.13ASLFWRR717 pKa = 11.84GEE719 pKa = 3.94RR720 pKa = 11.84LTLGPTLSKK729 pKa = 11.12APIGSASAEE738 pKa = 3.96DD739 pKa = 3.79LARR742 pKa = 11.84ATKK745 pKa = 9.9PHH747 pKa = 6.69GLLAQYY753 pKa = 10.24PLLLLARR760 pKa = 11.84DD761 pKa = 4.98RR762 pKa = 11.84IPEE765 pKa = 4.03TLVRR769 pKa = 11.84VALGKK774 pKa = 10.64AGGNPHH780 pKa = 6.59TSHH783 pKa = 7.5LAYY786 pKa = 10.29DD787 pKa = 3.29AWGEE791 pKa = 4.01YY792 pKa = 10.57DD793 pKa = 4.65HH794 pKa = 7.06GCQIQTVLGYY804 pKa = 11.03ADD806 pKa = 3.57AAAFGKK812 pKa = 8.69RR813 pKa = 11.84TSADD817 pKa = 3.13VLTSRR822 pKa = 11.84TLMYY826 pKa = 10.73VV827 pKa = 3.23

Molecular weight:
90.22 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1593

766

827

796.5

85.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.869 ± 0.576

1.318 ± 0.009

4.834 ± 0.087

4.52 ± 0.325

2.448 ± 0.381

8.349 ± 0.632

2.511 ± 0.158

2.637 ± 0.431

2.323 ± 0.34

9.605 ± 1.395

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.883 ± 0.128

3.013 ± 0.455

7.094 ± 1.583

2.825 ± 0.481

7.031 ± 0.525

7.596 ± 0.016

6.529 ± 0.358

7.847 ± 0.458

1.318 ± 0.278

3.453 ± 0.757

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski