Epichloe festucae virus 1
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q1M2S1|Q1M2S1_9VIRU Putative coat protein OS=Epichloe festucae virus 1 OX=382962 GN=CP PE=4 SV=1
MM1 pKa = 7.44 MSTAQTQNFQVRR13 pKa = 11.84 HH14 pKa = 5.85 LAPLTGAVADD24 pKa = 4.11 PVGGLLQRR32 pKa = 11.84 GKK34 pKa = 10.56 DD35 pKa = 3.51 FRR37 pKa = 11.84 RR38 pKa = 11.84 YY39 pKa = 9.5 RR40 pKa = 11.84 SGLSSEE46 pKa = 4.03 IPGPGRR52 pKa = 11.84 TFVEE56 pKa = 4.27 YY57 pKa = 10.45 RR58 pKa = 11.84 SIFYY62 pKa = 10.41 EE63 pKa = 4.06 VGRR66 pKa = 11.84 HH67 pKa = 5.01 YY68 pKa = 11.66 GNISDD73 pKa = 5.38 ALATPTPAAVRR84 pKa = 11.84 IDD86 pKa = 3.62 CSHH89 pKa = 7.65 TINEE93 pKa = 4.08 TQAANFEE100 pKa = 4.33 GLARR104 pKa = 11.84 QYY106 pKa = 11.73 SNFASQWEE114 pKa = 4.18 KK115 pKa = 10.7 MDD117 pKa = 3.44 LCGIAEE123 pKa = 4.08 RR124 pKa = 11.84 LARR127 pKa = 11.84 AIATQSVFGGVTTTHH142 pKa = 5.66 MRR144 pKa = 11.84 AGQPLRR150 pKa = 11.84 VVALGTLDD158 pKa = 3.82 SPQTASTSSVFIPRR172 pKa = 11.84 TVDD175 pKa = 3.29 SVGSDD180 pKa = 2.89 HH181 pKa = 7.03 VFAVLCAAANGEE193 pKa = 4.31 GASVSTDD200 pKa = 3.2 VLRR203 pKa = 11.84 LDD205 pKa = 3.95 VSNNAPLVPAVAGAPLATACVEE227 pKa = 4.0 ALRR230 pKa = 11.84 VLGANMEE237 pKa = 4.12 EE238 pKa = 4.62 SGAGDD243 pKa = 4.1 LFAYY247 pKa = 10.12 AVTRR251 pKa = 11.84 GIHH254 pKa = 5.2 RR255 pKa = 11.84 VVTVVSHH262 pKa = 5.84 TDD264 pKa = 2.73 EE265 pKa = 5.1 GGILRR270 pKa = 11.84 DD271 pKa = 4.06 LLRR274 pKa = 11.84 HH275 pKa = 5.8 DD276 pKa = 4.73 AFRR279 pKa = 11.84 VPYY282 pKa = 10.31 GGINASLRR290 pKa = 11.84 HH291 pKa = 5.31 YY292 pKa = 9.18 PALPSAAGSHH302 pKa = 6.11 EE303 pKa = 4.25 SSIAAWVDD311 pKa = 4.47 GIALKK316 pKa = 9.22 TAAVAAHH323 pKa = 7.18 CDD325 pKa = 3.59 PLVPGVGGMYY335 pKa = 7.99 PTVLVASKK343 pKa = 11.04 GSISPPGTPEE353 pKa = 4.35 GDD355 pKa = 3.47 VSPDD359 pKa = 3.35 DD360 pKa = 3.81 ARR362 pKa = 11.84 SLGRR366 pKa = 11.84 QLSADD371 pKa = 3.07 IGRR374 pKa = 11.84 FAPNYY379 pKa = 9.59 IRR381 pKa = 11.84 GLSALFGLQGYY392 pKa = 6.15 TAIAEE397 pKa = 4.15 RR398 pKa = 11.84 HH399 pKa = 5.06 LCATAGHH406 pKa = 5.72 YY407 pKa = 10.66 LEE409 pKa = 5.6 EE410 pKa = 4.41 NSATNRR416 pKa = 11.84 HH417 pKa = 5.95 LLHH420 pKa = 6.18 KK421 pKa = 9.49 TIAPYY426 pKa = 10.09 FWVEE430 pKa = 3.89 PTSLIPRR437 pKa = 11.84 DD438 pKa = 3.77 FLGTSAEE445 pKa = 4.23 SEE447 pKa = 4.04 NYY449 pKa = 8.32 AAKK452 pKa = 10.48 VSPGCVAHH460 pKa = 6.93 EE461 pKa = 3.8 PLFEE465 pKa = 4.31 KK466 pKa = 10.36 FAPLAQGTTMTCATAGFKK484 pKa = 9.69 MRR486 pKa = 11.84 TARR489 pKa = 11.84 TSGYY493 pKa = 9.99 VCSQAAAPASLAMIQLKK510 pKa = 9.86 QFDD513 pKa = 4.14 TQSVVLAGDD522 pKa = 3.36 QGPISGDD529 pKa = 3.15 VVTKK533 pKa = 10.43 HH534 pKa = 5.97 RR535 pKa = 11.84 ASSPLSSYY543 pKa = 10.78 LWVRR547 pKa = 11.84 GQSALPAPAEE557 pKa = 4.25 FINTQGCYY565 pKa = 8.63 GAKK568 pKa = 9.7 IKK570 pKa = 10.94 LVDD573 pKa = 3.86 WTDD576 pKa = 3.21 DD577 pKa = 3.38 WDD579 pKa = 4.29 PEE581 pKa = 4.47 STDD584 pKa = 3.45 VPRR587 pKa = 11.84 PDD589 pKa = 3.24 QFATGVVAWRR599 pKa = 11.84 VSLPTGLPTGPSNAADD615 pKa = 3.79 RR616 pKa = 11.84 QARR619 pKa = 11.84 RR620 pKa = 11.84 ARR622 pKa = 11.84 SRR624 pKa = 11.84 GAIALAQALNRR635 pKa = 11.84 ARR637 pKa = 11.84 AFGEE641 pKa = 4.47 GASPTMEE648 pKa = 4.03 VSDD651 pKa = 4.24 VPPDD655 pKa = 3.93 LGLADD660 pKa = 4.84 LGHH663 pKa = 6.59 YY664 pKa = 10.12 DD665 pKa = 3.11 HH666 pKa = 7.23 TGFRR670 pKa = 11.84 EE671 pKa = 3.93 HH672 pKa = 7.42 RR673 pKa = 11.84 SDD675 pKa = 3.39 PGKK678 pKa = 10.57 AQEE681 pKa = 4.62 TGDD684 pKa = 4.24 GNPSPTPTLLRR695 pKa = 11.84 GAAMPPTEE703 pKa = 3.78 QHH705 pKa = 6.31 KK706 pKa = 9.45 PQGGPRR712 pKa = 11.84 LPAPPPTMGRR722 pKa = 11.84 AAGPVGPPSTPAPPPPAPVIAPPTVSDD749 pKa = 4.28 PPPMPEE755 pKa = 3.73 AATGAEE761 pKa = 4.39 PVPQAA766 pKa = 4.04
Molecular weight: 80.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.015
IPC2_protein 5.919
IPC_protein 6.008
Toseland 6.427
ProMoST 6.415
Dawson 6.326
Bjellqvist 6.326
Wikipedia 6.351
Rodwell 6.326
Grimsley 6.59
Solomon 6.326
Lehninger 6.326
Nozaki 6.62
DTASelect 6.795
Thurlkill 6.825
EMBOSS 6.795
Sillero 6.722
Patrickios 4.202
IPC_peptide 6.351
IPC2_peptide 6.707
IPC2.peptide.svr19 6.612
Protein with the highest isoelectric point:
>tr|Q1M2S1|Q1M2S1_9VIRU Putative coat protein OS=Epichloe festucae virus 1 OX=382962 GN=CP PE=4 SV=1
MM1 pKa = 7.55 IEE3 pKa = 3.94 STVADD8 pKa = 4.17 RR9 pKa = 11.84 ANAAGSVGRR18 pKa = 11.84 YY19 pKa = 9.4 LEE21 pKa = 4.35 ALLPSEE27 pKa = 4.2 YY28 pKa = 10.09 TRR30 pKa = 11.84 VVSTLPFDD38 pKa = 3.8 HH39 pKa = 6.37 QVSFVYY45 pKa = 10.27 RR46 pKa = 11.84 PSWRR50 pKa = 11.84 HH51 pKa = 5.14 HH52 pKa = 6.54 KK53 pKa = 8.77 PTEE56 pKa = 4.0 LQRR59 pKa = 11.84 VAAAFLLPRR68 pKa = 11.84 VPVQVSYY75 pKa = 11.13 SAGSILTLLNATLPNLPPRR94 pKa = 11.84 HH95 pKa = 6.11 TYY97 pKa = 8.72 HH98 pKa = 7.15 AGWSCDD104 pKa = 2.97 SDD106 pKa = 3.55 ATRR109 pKa = 11.84 KK110 pKa = 9.78 AFPLKK115 pKa = 10.69 ANPAASNKK123 pKa = 8.79 VNVYY127 pKa = 10.31 LSEE130 pKa = 3.87 VCYY133 pKa = 10.4 DD134 pKa = 3.9 LRR136 pKa = 11.84 RR137 pKa = 11.84 TNPGEE142 pKa = 4.0 AMAAEE147 pKa = 4.57 AALQRR152 pKa = 11.84 VRR154 pKa = 11.84 SSGSVYY160 pKa = 10.56 NDD162 pKa = 2.77 QATAVVLYY170 pKa = 7.78 GFCLARR176 pKa = 11.84 NGLPGAYY183 pKa = 9.84 DD184 pKa = 3.06 IALALLLNPDD194 pKa = 3.73 YY195 pKa = 11.1 AKK197 pKa = 10.96 SLTGVLKK204 pKa = 10.09 ATGGNATAVGSLLVEE219 pKa = 4.5 ANTLLGRR226 pKa = 11.84 DD227 pKa = 3.85 VAPVDD232 pKa = 3.83 LGKK235 pKa = 10.38 EE236 pKa = 4.14 FNMRR240 pKa = 11.84 TQLRR244 pKa = 11.84 LARR247 pKa = 11.84 EE248 pKa = 4.03 NMAEE252 pKa = 4.09 YY253 pKa = 9.98 PPDD256 pKa = 3.5 VLRR259 pKa = 11.84 ATVRR263 pKa = 11.84 RR264 pKa = 11.84 VLLDD268 pKa = 3.2 EE269 pKa = 4.07 LTRR272 pKa = 11.84 RR273 pKa = 11.84 AGSYY277 pKa = 10.01 EE278 pKa = 4.08 LDD280 pKa = 3.63 YY281 pKa = 11.2 PSLEE285 pKa = 4.25 EE286 pKa = 4.1 HH287 pKa = 6.8 WDD289 pKa = 3.81 SRR291 pKa = 11.84 WAWAVNGAQGGAITDD306 pKa = 3.9 KK307 pKa = 10.91 GLEE310 pKa = 4.18 SAPRR314 pKa = 11.84 PPGATRR320 pKa = 11.84 EE321 pKa = 4.07 YY322 pKa = 9.88 RR323 pKa = 11.84 RR324 pKa = 11.84 SWLEE328 pKa = 3.65 RR329 pKa = 11.84 VEE331 pKa = 4.12 EE332 pKa = 4.21 DD333 pKa = 5.49 PRR335 pKa = 11.84 PQWDD339 pKa = 3.5 GTTEE343 pKa = 3.89 VSASSKK349 pKa = 10.62 LEE351 pKa = 3.94 HH352 pKa = 6.53 GKK354 pKa = 7.98 TRR356 pKa = 11.84 AIFACDD362 pKa = 3.2 TVNYY366 pKa = 10.14 LAFEE370 pKa = 4.5 HH371 pKa = 6.76 LMSTVEE377 pKa = 4.06 ANWRR381 pKa = 11.84 GVRR384 pKa = 11.84 VVLNPGKK391 pKa = 10.15 GGNMGMAMRR400 pKa = 11.84 VQAARR405 pKa = 11.84 RR406 pKa = 11.84 RR407 pKa = 11.84 CGVSLMLDD415 pKa = 3.2 YY416 pKa = 11.57 DD417 pKa = 4.5 DD418 pKa = 5.99 FNSHH422 pKa = 5.4 HH423 pKa = 7.22 TISAMQIVVEE433 pKa = 4.56 EE434 pKa = 4.46 VCSITGYY441 pKa = 11.11 DD442 pKa = 3.48 PDD444 pKa = 3.59 LAARR448 pKa = 11.84 LVASLDD454 pKa = 3.42 KK455 pKa = 11.09 QYY457 pKa = 9.7 ITLAGRR463 pKa = 11.84 RR464 pKa = 11.84 KK465 pKa = 10.21 LSVGTLMSGHH475 pKa = 6.91 RR476 pKa = 11.84 CTTFFNSVLNAVYY489 pKa = 10.43 VRR491 pKa = 11.84 LEE493 pKa = 4.13 LGEE496 pKa = 4.22 PFYY499 pKa = 11.48 SEE501 pKa = 4.26 TVSLHH506 pKa = 5.96 VGDD509 pKa = 4.41 DD510 pKa = 3.37 VYY512 pKa = 10.99 MGVRR516 pKa = 11.84 DD517 pKa = 4.23 YY518 pKa = 11.86 VSAAYY523 pKa = 9.86 VIQRR527 pKa = 11.84 LADD530 pKa = 3.29 SPLRR534 pKa = 11.84 MNALKK539 pKa = 10.59 QSVGHH544 pKa = 6.11 VSTEE548 pKa = 3.76 FLRR551 pKa = 11.84 IATGARR557 pKa = 11.84 DD558 pKa = 3.57 CYY560 pKa = 10.89 GYY562 pKa = 10.97 LSRR565 pKa = 11.84 AVASIVSGNWVNDD578 pKa = 3.31 TALAPFEE585 pKa = 4.52 GLAAMVASARR595 pKa = 11.84 SLANRR600 pKa = 11.84 GKK602 pKa = 9.19 TNLAPLLLVSSVLRR616 pKa = 11.84 IAKK619 pKa = 9.69 LGKK622 pKa = 8.53 RR623 pKa = 11.84 HH624 pKa = 5.43 YY625 pKa = 10.56 GKK627 pKa = 10.62 VSSLLDD633 pKa = 3.32 GTVALGNGPQYY644 pKa = 11.27 SCGAHH649 pKa = 5.51 MRR651 pKa = 11.84 WCNPVVVRR659 pKa = 11.84 DD660 pKa = 4.03 TPDD663 pKa = 2.67 PWGYY667 pKa = 9.12 TEE669 pKa = 5.9 LPLEE673 pKa = 4.36 SVTAYY678 pKa = 10.69 LSRR681 pKa = 11.84 AASPLEE687 pKa = 3.91 WEE689 pKa = 4.6 VLGEE693 pKa = 4.01 AGISVTEE700 pKa = 3.89 QMAAASYY707 pKa = 9.53 RR708 pKa = 11.84 KK709 pKa = 8.18 TYY711 pKa = 11.13 ASLFWRR717 pKa = 11.84 GEE719 pKa = 3.94 RR720 pKa = 11.84 LTLGPTLSKK729 pKa = 11.12 APIGSASAEE738 pKa = 3.96 DD739 pKa = 3.79 LARR742 pKa = 11.84 ATKK745 pKa = 9.9 PHH747 pKa = 6.69 GLLAQYY753 pKa = 10.24 PLLLLARR760 pKa = 11.84 DD761 pKa = 4.98 RR762 pKa = 11.84 IPEE765 pKa = 4.03 TLVRR769 pKa = 11.84 VALGKK774 pKa = 10.64 AGGNPHH780 pKa = 6.59 TSHH783 pKa = 7.5 LAYY786 pKa = 10.29 DD787 pKa = 3.29 AWGEE791 pKa = 4.01 YY792 pKa = 10.57 DD793 pKa = 4.65 HH794 pKa = 7.06 GCQIQTVLGYY804 pKa = 11.03 ADD806 pKa = 3.57 AAAFGKK812 pKa = 8.69 RR813 pKa = 11.84 TSADD817 pKa = 3.13 VLTSRR822 pKa = 11.84 TLMYY826 pKa = 10.73 VV827 pKa = 3.23
Molecular weight: 90.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.496
IPC2_protein 7.819
IPC_protein 7.673
Toseland 7.366
ProMoST 8.185
Dawson 8.331
Bjellqvist 8.653
Wikipedia 8.287
Rodwell 8.346
Grimsley 7.439
Solomon 8.404
Lehninger 8.404
Nozaki 8.697
DTASelect 8.419
Thurlkill 8.448
EMBOSS 8.521
Sillero 8.741
Patrickios 4.19
IPC_peptide 8.39
IPC2_peptide 7.717
IPC2.peptide.svr19 7.831
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1593
766
827
796.5
85.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.869 ± 0.576
1.318 ± 0.009
4.834 ± 0.087
4.52 ± 0.325
2.448 ± 0.381
8.349 ± 0.632
2.511 ± 0.158
2.637 ± 0.431
2.323 ± 0.34
9.605 ± 1.395
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.883 ± 0.128
3.013 ± 0.455
7.094 ± 1.583
2.825 ± 0.481
7.031 ± 0.525
7.596 ± 0.016
6.529 ± 0.358
7.847 ± 0.458
1.318 ± 0.278
3.453 ± 0.757
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here