Pseudoarcobacter caeni
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2336 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A363D5Z3|A0A363D5Z3_9PROT Nitrogen fixation protein NifU OS=Pseudoarcobacter caeni OX=1912877 GN=B0174_01270 PE=3 SV=1
MM1 pKa = 7.73 EE2 pKa = 4.62 PTFDD6 pKa = 4.64 LKK8 pKa = 11.03 EE9 pKa = 4.1 IEE11 pKa = 4.37 NKK13 pKa = 10.58 LIEE16 pKa = 4.43 HH17 pKa = 6.86 LKK19 pKa = 10.43 KK20 pKa = 11.01 VYY22 pKa = 10.5 DD23 pKa = 3.86 PEE25 pKa = 5.44 IPTNIYY31 pKa = 10.8 DD32 pKa = 4.55 LGLIYY37 pKa = 10.73 NIEE40 pKa = 3.82 FDD42 pKa = 3.9 VNNNYY47 pKa = 10.38 LYY49 pKa = 9.94 CTVTMTLTSPTCPVADD65 pKa = 3.95 SLLEE69 pKa = 3.8 QVKK72 pKa = 9.69 YY73 pKa = 9.11 VTLAVDD79 pKa = 4.81 EE80 pKa = 4.41 IDD82 pKa = 3.51 EE83 pKa = 4.57 AFVKK87 pKa = 10.61 LVFSPPWDD95 pKa = 3.6 PSMMSEE101 pKa = 4.15 DD102 pKa = 3.49 AKK104 pKa = 11.09 EE105 pKa = 3.79 IMGASGAAMAFF116 pKa = 3.66
Molecular weight: 13.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.823
IPC2_protein 4.062
IPC_protein 3.986
Toseland 3.795
ProMoST 4.113
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.846
Rodwell 3.821
Grimsley 3.706
Solomon 3.935
Lehninger 3.897
Nozaki 4.062
DTASelect 4.228
Thurlkill 3.834
EMBOSS 3.859
Sillero 4.101
Patrickios 1.914
IPC_peptide 3.935
IPC2_peptide 4.075
IPC2.peptide.svr19 3.986
Protein with the highest isoelectric point:
>tr|A0A363D2N3|A0A363D2N3_9PROT DNA-binding response regulator OS=Pseudoarcobacter caeni OX=1912877 GN=B0174_04425 PE=4 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.04 QPHH8 pKa = 4.86 NTPRR12 pKa = 11.84 KK13 pKa = 7.31 RR14 pKa = 11.84 THH16 pKa = 5.83 GFRR19 pKa = 11.84 VRR21 pKa = 11.84 MATKK25 pKa = 9.98 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VINRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.09 GRR39 pKa = 11.84 HH40 pKa = 5.01 KK41 pKa = 10.78 LAVV44 pKa = 3.35
Molecular weight: 5.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2336
0
2336
746209
24
8556
319.4
36.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.812 ± 0.062
0.9 ± 0.021
5.69 ± 0.046
7.097 ± 0.073
5.325 ± 0.048
5.368 ± 0.11
1.531 ± 0.023
9.899 ± 0.052
9.348 ± 0.084
9.698 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.288 ± 0.029
6.707 ± 0.069
2.649 ± 0.035
2.815 ± 0.023
2.77 ± 0.039
6.612 ± 0.046
5.175 ± 0.069
5.697 ± 0.043
0.693 ± 0.015
3.925 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here