Anaerotruncus sp. AF02-27
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3622 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A413G8V3|A0A413G8V3_9FIRM Urea ABC transporter substrate-binding protein OS=Anaerotruncus sp. AF02-27 OX=2292191 GN=DWV16_01135 PE=4 SV=1
MM1 pKa = 7.21 FTPKK5 pKa = 10.31 NIQGALEE12 pKa = 4.07 EE13 pKa = 5.47 LYY15 pKa = 10.79 DD16 pKa = 4.61 LCDD19 pKa = 3.77 PDD21 pKa = 5.15 YY22 pKa = 10.8 MVDD25 pKa = 3.24 MLVNYY30 pKa = 10.23 SEE32 pKa = 4.6 EE33 pKa = 4.12 FDD35 pKa = 5.3 DD36 pKa = 5.77 ISPALLAKK44 pKa = 10.25 SFQKK48 pKa = 9.71 NAEE51 pKa = 4.23 MISEE55 pKa = 4.04 YY56 pKa = 10.38 RR57 pKa = 11.84 VLSSAGEE64 pKa = 4.39 GIDD67 pKa = 3.7 YY68 pKa = 10.26 QGKK71 pKa = 9.06 VLLNSRR77 pKa = 11.84 AVRR80 pKa = 11.84 LLSYY84 pKa = 10.75 VEE86 pKa = 5.09 DD87 pKa = 3.48 MSGDD91 pKa = 3.5 EE92 pKa = 4.42 KK93 pKa = 11.44 VRR95 pKa = 11.84 TIQSKK100 pKa = 7.86 EE101 pKa = 3.44 LWLAEE106 pKa = 4.02 DD107 pKa = 3.22 MTFYY111 pKa = 11.0 VVSCMSTITMDD122 pKa = 3.5 KK123 pKa = 11.1 EE124 pKa = 3.65 EE125 pKa = 4.92 AICLNEE131 pKa = 3.85 HH132 pKa = 6.46 RR133 pKa = 11.84 SVVTTVEE140 pKa = 4.18 CEE142 pKa = 3.67 DD143 pKa = 5.83 DD144 pKa = 3.43 IFFDD148 pKa = 3.85 MGSLICEE155 pKa = 4.64 LDD157 pKa = 4.38 DD158 pKa = 3.63 ICLFEE163 pKa = 5.52 LLADD167 pKa = 4.03 VDD169 pKa = 3.72 ATIYY173 pKa = 10.61 EE174 pKa = 4.33 LL175 pKa = 4.55
Molecular weight: 19.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.725
IPC2_protein 3.884
IPC_protein 3.859
Toseland 3.656
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.745
Rodwell 3.694
Grimsley 3.567
Solomon 3.821
Lehninger 3.783
Nozaki 3.948
DTASelect 4.139
Thurlkill 3.694
EMBOSS 3.757
Sillero 3.973
Patrickios 1.125
IPC_peptide 3.834
IPC2_peptide 3.961
IPC2.peptide.svr19 3.875
Protein with the highest isoelectric point:
>tr|A0A413G5I7|A0A413G5I7_9FIRM Cob(I)yrinic acid a c-diamide adenosyltransferase OS=Anaerotruncus sp. AF02-27 OX=2292191 GN=DWV16_06465 PE=4 SV=1
MM1 pKa = 7.64 TNQFHH6 pKa = 7.11 PGNRR10 pKa = 11.84 PGRR13 pKa = 11.84 PDD15 pKa = 4.73 RR16 pKa = 11.84 PMQPNPQPANPNPQQAPQNQQAQLNQMRR44 pKa = 11.84 MQQNFQNRR52 pKa = 11.84 NGMRR56 pKa = 11.84 MQQNHH61 pKa = 5.36 GNMSRR66 pKa = 11.84 MNQNRR71 pKa = 11.84 QNPSGVQKK79 pKa = 10.62 ILQTALQNPVGTLSKK94 pKa = 10.83 VMNIIRR100 pKa = 11.84 LLGG103 pKa = 3.64
Molecular weight: 11.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.367
IPC2_protein 10.789
IPC_protein 12.384
Toseland 12.544
ProMoST 13.042
Dawson 12.544
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.149
Grimsley 12.588
Solomon 13.042
Lehninger 12.939
Nozaki 12.544
DTASelect 12.544
Thurlkill 12.544
EMBOSS 13.042
Sillero 12.544
Patrickios 11.901
IPC_peptide 13.042
IPC2_peptide 12.032
IPC2.peptide.svr19 9.138
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3622
0
3622
1085505
19
2927
299.7
33.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.496 ± 0.04
1.66 ± 0.017
5.54 ± 0.037
6.717 ± 0.043
4.057 ± 0.028
7.704 ± 0.036
1.731 ± 0.019
6.402 ± 0.039
5.349 ± 0.038
9.567 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.929 ± 0.023
3.508 ± 0.021
4.245 ± 0.023
3.381 ± 0.025
5.405 ± 0.042
5.679 ± 0.033
5.218 ± 0.028
7.037 ± 0.037
0.916 ± 0.014
3.461 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here