Sulfuritortus calidifontis
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2668 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R3JWV7|A0A4R3JWV7_9PROT Glycine oxidase OS=Sulfuritortus calidifontis OX=1914471 GN=EDC61_104125 PE=4 SV=1
MM1 pKa = 7.86 ASQLIGTTEE10 pKa = 3.84 IGWFNPSVTPPPFDD24 pKa = 3.25 MKK26 pKa = 10.94 LLLMVAGSRR35 pKa = 11.84 SEE37 pKa = 4.09 DD38 pKa = 3.37 CGRR41 pKa = 11.84 TFEE44 pKa = 4.83 PYY46 pKa = 8.78 TVVMTAYY53 pKa = 9.94 VQQQGPGDD61 pKa = 3.94 EE62 pKa = 4.76 YY63 pKa = 11.6 DD64 pKa = 4.77 DD65 pKa = 3.42 EE66 pKa = 4.76 TAFDD70 pKa = 4.6 EE71 pKa = 4.96 YY72 pKa = 10.95 MSGDD76 pKa = 3.29 QTDD79 pKa = 4.0 FLDD82 pKa = 4.16 FQFDD86 pKa = 3.8 LKK88 pKa = 11.43 DD89 pKa = 3.39 EE90 pKa = 4.9 DD91 pKa = 5.28 GEE93 pKa = 4.4 VLDD96 pKa = 4.93 WYY98 pKa = 10.74 SDD100 pKa = 4.15 SIVAWAYY107 pKa = 9.85 YY108 pKa = 9.59 PLGIAQTAVNVEE120 pKa = 4.29 RR121 pKa = 11.84 EE122 pKa = 3.95 RR123 pKa = 11.84 QAALVPNVMM132 pKa = 4.64
Molecular weight: 14.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.747
IPC2_protein 3.617
IPC_protein 3.592
Toseland 3.389
ProMoST 3.77
Dawson 3.592
Bjellqvist 3.757
Wikipedia 3.541
Rodwell 3.427
Grimsley 3.3
Solomon 3.579
Lehninger 3.528
Nozaki 3.719
DTASelect 3.935
Thurlkill 3.452
EMBOSS 3.554
Sillero 3.719
Patrickios 0.846
IPC_peptide 3.567
IPC2_peptide 3.694
IPC2.peptide.svr19 3.689
Protein with the highest isoelectric point:
>tr|A0A4R3JTH8|A0A4R3JTH8_9PROT ATP synthase protein I OS=Sulfuritortus calidifontis OX=1914471 GN=EDC61_11447 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.07 QPSKK9 pKa = 9.07 IRR11 pKa = 11.84 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.96 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 GGRR28 pKa = 11.84 AVINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.58 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2668
0
2668
822929
27
4205
308.4
33.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.383 ± 0.063
0.929 ± 0.016
5.247 ± 0.043
6.265 ± 0.045
3.464 ± 0.031
8.121 ± 0.057
2.318 ± 0.024
4.814 ± 0.036
3.839 ± 0.05
11.275 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.391 ± 0.024
2.701 ± 0.046
5.029 ± 0.038
3.877 ± 0.031
7.028 ± 0.063
4.645 ± 0.034
4.611 ± 0.044
7.113 ± 0.042
1.418 ± 0.022
2.531 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here