Arcobacter butzleri (strain RM4018)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; Campylobacterales; Campylobacteraceae; Arcobacter group; Aliarcobacter; Aliarcobacter butzleri

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2254 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A8EU35|A8EU35_ARCB4 Aspartokinase OS=Arcobacter butzleri (strain RM4018) OX=367737 GN=lysC PE=3 SV=1
MM1 pKa = 7.32IVSIDD6 pKa = 3.38KK7 pKa = 10.85NYY9 pKa = 10.38LFNIFGVNNFSSLEE23 pKa = 4.1TIIDD27 pKa = 3.56SMAPSLVEE35 pKa = 3.51YY36 pKa = 9.98HH37 pKa = 6.66LSSFCEE43 pKa = 4.3DD44 pKa = 3.46NSDD47 pKa = 3.53TIYY50 pKa = 10.7FNKK53 pKa = 9.95RR54 pKa = 11.84DD55 pKa = 3.68VEE57 pKa = 4.19KK58 pKa = 11.12SFLAGDD64 pKa = 3.43YY65 pKa = 9.94TLYY68 pKa = 11.04LDD70 pKa = 3.99YY71 pKa = 11.46SEE73 pKa = 5.35NLYY76 pKa = 11.14LEE78 pKa = 5.59LIEE81 pKa = 5.3SDD83 pKa = 3.44IDD85 pKa = 4.04EE86 pKa = 4.45MTQSFWW92 pKa = 3.96

Molecular weight: 10.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A8EVA6|A8EVA6_ARCB4 Peptide-methionine (R)-S-oxide reductase OS=Arcobacter butzleri (strain RM4018) OX=367737 GN=msrB PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.04QPHH8 pKa = 4.86NTPRR12 pKa = 11.84KK13 pKa = 7.31RR14 pKa = 11.84THH16 pKa = 5.83GFRR19 pKa = 11.84VRR21 pKa = 11.84MATKK25 pKa = 9.98NGRR28 pKa = 11.84RR29 pKa = 11.84VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2254

0

2254

722876

30

3033

320.7

36.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.784 ± 0.053

0.844 ± 0.018

5.649 ± 0.042

7.151 ± 0.048

5.443 ± 0.052

5.319 ± 0.056

1.43 ± 0.021

9.729 ± 0.062

9.528 ± 0.056

9.732 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.21 ± 0.026

6.713 ± 0.067

2.651 ± 0.029

2.819 ± 0.026

2.74 ± 0.033

6.641 ± 0.043

5.172 ± 0.06

5.788 ± 0.041

0.688 ± 0.015

3.969 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski