Archaeoglobus veneficus (strain DSM 11195 / SNP6)
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2065 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F2KN46|F2KN46_ARCVS Uncharacterized protein OS=Archaeoglobus veneficus (strain DSM 11195 / SNP6) OX=693661 GN=Arcve_0106 PE=4 SV=1
MM1 pKa = 7.75 RR2 pKa = 11.84 NILVSLLLLGFAAALVGSGTLAYY25 pKa = 10.38 FSDD28 pKa = 4.19 TEE30 pKa = 4.15 TSEE33 pKa = 4.42 NNVFGAGMIDD43 pKa = 4.04 ISIDD47 pKa = 4.28 DD48 pKa = 4.17 EE49 pKa = 4.64 NPWISNYY56 pKa = 8.11 TMEE59 pKa = 5.17 DD60 pKa = 3.55 LKK62 pKa = 11.03 PCEE65 pKa = 4.14 TGWINFTIKK74 pKa = 10.52 NLEE77 pKa = 3.81 KK78 pKa = 10.42 DD79 pKa = 4.22 DD80 pKa = 3.82 PVKK83 pKa = 10.25 IYY85 pKa = 10.97 KK86 pKa = 9.9 HH87 pKa = 6.19 INITGYY93 pKa = 10.67 DD94 pKa = 3.56 GGLHH98 pKa = 6.53 PEE100 pKa = 4.13 SEE102 pKa = 4.36 EE103 pKa = 4.99 AEE105 pKa = 4.25 DD106 pKa = 6.0 LNDD109 pKa = 4.35 EE110 pKa = 4.6 INDD113 pKa = 3.16 IGKK116 pKa = 9.86 NITYY120 pKa = 9.38 DD121 pKa = 3.42 LHH123 pKa = 7.01 VAIYY127 pKa = 9.9 NEE129 pKa = 4.1 TGALVHH135 pKa = 6.17 EE136 pKa = 4.5 EE137 pKa = 4.09 WIIEE141 pKa = 3.9 EE142 pKa = 4.35 TEE144 pKa = 4.0 NVKK147 pKa = 10.41 VEE149 pKa = 5.83 DD150 pKa = 3.86 IDD152 pKa = 5.28 CYY154 pKa = 10.35 WIYY157 pKa = 11.02 LGDD160 pKa = 3.75 IPQNGTMVVNQSYY173 pKa = 10.74 HH174 pKa = 4.88 MQPEE178 pKa = 4.34 VTNWAQGDD186 pKa = 3.36 IMYY189 pKa = 10.5 FDD191 pKa = 4.37 IEE193 pKa = 4.34 VLATQTNAPAPSPLWPP209 pKa = 4.45
Molecular weight: 23.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.781
IPC2_protein 3.897
IPC_protein 3.872
Toseland 3.681
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.745
Rodwell 3.706
Grimsley 3.592
Solomon 3.834
Lehninger 3.795
Nozaki 3.961
DTASelect 4.139
Thurlkill 3.719
EMBOSS 3.757
Sillero 3.986
Patrickios 0.668
IPC_peptide 3.834
IPC2_peptide 3.973
IPC2.peptide.svr19 3.88
Protein with the highest isoelectric point:
>tr|F2KR41|F2KR41_ARCVS Other/FunK1 protein kinase OS=Archaeoglobus veneficus (strain DSM 11195 / SNP6) OX=693661 GN=Arcve_0658 PE=4 SV=1
MM1 pKa = 7.34 GKK3 pKa = 8.36 KK4 pKa = 8.33 TVGVKK9 pKa = 10.11 KK10 pKa = 10.52 RR11 pKa = 11.84 LAKK14 pKa = 10.07 FLKK17 pKa = 8.88 QNRR20 pKa = 11.84 RR21 pKa = 11.84 APVWITLKK29 pKa = 9.31 TKK31 pKa = 10.37 RR32 pKa = 11.84 RR33 pKa = 11.84 VFGSPKK39 pKa = 8.46 RR40 pKa = 11.84 RR41 pKa = 11.84 NWRR44 pKa = 11.84 VTKK47 pKa = 10.74 LKK49 pKa = 10.8 VV50 pKa = 2.92
Molecular weight: 5.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.492
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.749
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.486
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.066
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2065
0
2065
558359
31
2522
270.4
30.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.854 ± 0.066
1.275 ± 0.029
5.057 ± 0.034
8.696 ± 0.08
4.231 ± 0.044
7.088 ± 0.044
1.624 ± 0.023
7.756 ± 0.045
7.008 ± 0.06
9.382 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.52 ± 0.027
3.222 ± 0.049
3.872 ± 0.028
1.748 ± 0.024
5.752 ± 0.055
5.403 ± 0.052
4.35 ± 0.05
8.618 ± 0.046
0.981 ± 0.02
3.564 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here