Jeotgalicoccus saudimassiliensis
Average proteome isoelectric point is 5.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2181 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A078MED2|A0A078MED2_9STAP D-alanyl-D-alanine carboxypeptidase OS=Jeotgalicoccus saudimassiliensis OX=1461582 GN=vanY PE=4 SV=1
MM1 pKa = 6.65 STKK4 pKa = 10.53 NFLIMVGGILAVLFFLLALAIRR26 pKa = 11.84 AEE28 pKa = 4.17 MGGDD32 pKa = 3.5 DD33 pKa = 4.07 TGTAGKK39 pKa = 8.04 TAGDD43 pKa = 3.64 TEE45 pKa = 4.34 EE46 pKa = 4.22 QQTEE50 pKa = 4.42 TEE52 pKa = 4.29 SQEE55 pKa = 3.98 TALNLNDD62 pKa = 3.58 SPLYY66 pKa = 10.29 AVHH69 pKa = 7.33 EE70 pKa = 4.29 DD71 pKa = 3.56 TAPVFQYY78 pKa = 10.97 QSGVDD83 pKa = 3.15 IPYY86 pKa = 10.02 PEE88 pKa = 4.69 EE89 pKa = 4.14 GVKK92 pKa = 10.55 GIYY95 pKa = 7.99 VTAYY99 pKa = 8.8 SAGGEE104 pKa = 4.08 RR105 pKa = 11.84 MPEE108 pKa = 4.6 LIDD111 pKa = 3.95 LVNNTGLNSMVIDD124 pKa = 3.89 VKK126 pKa = 11.23 EE127 pKa = 4.59 DD128 pKa = 2.87 IGDD131 pKa = 3.15 IMMPLDD137 pKa = 3.68 VDD139 pKa = 3.43 NDD141 pKa = 3.2 IVRR144 pKa = 11.84 NHH146 pKa = 6.35 MYY148 pKa = 10.58 DD149 pKa = 3.54 YY150 pKa = 11.19 VDD152 pKa = 4.32 PKK154 pKa = 11.61 ALMTTMEE161 pKa = 4.36 EE162 pKa = 4.04 NEE164 pKa = 4.39 IYY166 pKa = 10.22 PIARR170 pKa = 11.84 IVVFKK175 pKa = 10.57 DD176 pKa = 2.7 SRR178 pKa = 11.84 LAMEE182 pKa = 5.26 RR183 pKa = 11.84 PDD185 pKa = 4.91 LSYY188 pKa = 11.31 LNPDD192 pKa = 2.89 GSVWQNGSGEE202 pKa = 4.41 SFVNPFLKK210 pKa = 10.41 EE211 pKa = 3.71 VWDD214 pKa = 4.05 YY215 pKa = 11.61 NVDD218 pKa = 3.66 VAIEE222 pKa = 3.81 AAKK225 pKa = 10.72 LGFKK229 pKa = 9.94 EE230 pKa = 3.86 IQFDD234 pKa = 3.86 YY235 pKa = 11.38 VRR237 pKa = 11.84 FPEE240 pKa = 4.58 SFDD243 pKa = 3.63 TLSSGLTYY251 pKa = 11.02 DD252 pKa = 3.4 FGEE255 pKa = 4.5 YY256 pKa = 10.89 ANTEE260 pKa = 3.9 ADD262 pKa = 3.37 EE263 pKa = 4.23 VQQRR267 pKa = 11.84 VNAVTDD273 pKa = 4.36 FVAYY277 pKa = 10.1 ASEE280 pKa = 3.97 QLKK283 pKa = 10.61 PYY285 pKa = 10.5 DD286 pKa = 3.32 VDD288 pKa = 3.61 VSVDD292 pKa = 3.43 VFGYY296 pKa = 10.81 AATQRR301 pKa = 11.84 EE302 pKa = 4.44 APGIGQNFSQIADD315 pKa = 3.56 NVDD318 pKa = 3.9 IISSMIYY325 pKa = 8.5 PSHH328 pKa = 6.27 WGAYY332 pKa = 9.46 SFDD335 pKa = 3.15 IAAPDD340 pKa = 3.76 TEE342 pKa = 4.82 PYY344 pKa = 10.95 AVVDD348 pKa = 3.09 QYY350 pKa = 11.61 MKK352 pKa = 11.02 VEE354 pKa = 3.98 NEE356 pKa = 3.89 VLGVLEE362 pKa = 4.99 EE363 pKa = 4.52 PPEE366 pKa = 4.12 SRR368 pKa = 11.84 PWIQDD373 pKa = 2.74 FTAGDD378 pKa = 4.57 LGPGNYY384 pKa = 8.56 IEE386 pKa = 4.69 YY387 pKa = 10.06 NAPEE391 pKa = 4.25 VEE393 pKa = 4.2 AQIQALKK400 pKa = 11.1 DD401 pKa = 3.58 NGIDD405 pKa = 5.75 EE406 pKa = 4.46 YY407 pKa = 11.77 LLWNAQNEE415 pKa = 4.39 YY416 pKa = 11.03 SEE418 pKa = 4.27 GTEE421 pKa = 4.05 YY422 pKa = 10.86 QQ423 pKa = 3.2
Molecular weight: 47.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.71
IPC2_protein 3.808
IPC_protein 3.808
Toseland 3.605
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.681
Rodwell 3.643
Grimsley 3.516
Solomon 3.783
Lehninger 3.732
Nozaki 3.897
DTASelect 4.088
Thurlkill 3.643
EMBOSS 3.706
Sillero 3.923
Patrickios 1.1
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.826
Protein with the highest isoelectric point:
>tr|A0A078MCC8|A0A078MCC8_9STAP YciI-like protein OS=Jeotgalicoccus saudimassiliensis OX=1461582 GN=BN1048_01969 PE=3 SV=1
MM1 pKa = 7.49 VKK3 pKa = 9.1 RR4 pKa = 11.84 TYY6 pKa = 10.42 QPNKK10 pKa = 8.33 RR11 pKa = 11.84 KK12 pKa = 9.38 RR13 pKa = 11.84 AKK15 pKa = 9.1 VHH17 pKa = 5.5 GFRR20 pKa = 11.84 EE21 pKa = 4.12 RR22 pKa = 11.84 MSTKK26 pKa = 10.23 NGRR29 pKa = 11.84 KK30 pKa = 8.49 VLARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.05 GRR40 pKa = 11.84 KK41 pKa = 8.7 VLSAA45 pKa = 4.05
Molecular weight: 5.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.337
IPC2_protein 10.877
IPC_protein 12.281
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.223
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 11.959
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.025
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2181
0
2181
655108
30
1427
300.4
33.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.081 ± 0.061
0.512 ± 0.013
6.035 ± 0.048
7.802 ± 0.07
4.56 ± 0.038
6.869 ± 0.054
2.076 ± 0.025
8.044 ± 0.058
6.312 ± 0.054
9.3 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.984 ± 0.024
4.868 ± 0.04
3.334 ± 0.028
2.956 ± 0.031
3.933 ± 0.045
5.972 ± 0.039
5.79 ± 0.028
7.074 ± 0.044
0.739 ± 0.014
3.76 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here